Rroxscaffold_4G00324090

DNAJ heat shock N-terminal domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001405
Physical Location & Seq
Forward (+)
55395298 .. 55395907
610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324090.1

Sequence Viewer

Length: 582 bp
ATGGCCTCTGCATCGTCCCGGAATAATCAAGAGGCTGCTACCAAGGGACGAGACGCAGCGAGAAGCCTATTTCAAGCTCGGAAACTTGGGCTCGCAATCAATGAGGCCATGGAAGCTAAAAACCACGACCCGGATTTGCCCTATATCGACAATTTCATCACGGCTTACGGGATTCACCAAGCCGTGTCGGACAAGAAAAGGCGTGACTCTCAAACCATTAAGAAACAATACAAGGAATTGGCACTAGTTGTGCACCCCGACAAGAACAGCTCTCTCGCCGCCGACGGTGCTTTCGAGCATGTCAAAACCGCCTTGGACATTCTCTCCGACCCCCAACAAAAGGAGGATTACGACAAATCATTGAATCGTCGTGGTTACGGTAAGTCTTTTTCCGACGGGCATCGTACACGGTTTCATAGGTTTAATGATGATGATGATGATTATGTTCGAAAGTCGGGTGCAAGCTCACTACACCAATTTTGTTATCGGACAACGGCGAGAAAACCGTTGTGTGATTTGAAGACCCACCGTTGTAGGAGTGAGCCGTTGTCGATGAAAAATCGGACAACGGTGGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

193

Amino Acids

21.97

Weight (kDa)

9.3

Isoelectric Point (pI)

29.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 66 - 118 1.8e-14 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 279, 309
AfaI GTAC 1 cut(s) 406
AfiI CCNNNNNNNGG 2 cut(s) 130, 340
AgsI TTSAA 3 cut(s) 74, 364, 520
AhlI ACTAGT 1 cut(s) 244
AluBI AGCT 4 cut(s) 77, 116, 270, 465
AluI AGCT 4 cut(s) 77, 116, 270, 465
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 45
Alw44I GTGCAC 1 cut(s) 251
AoxI GGCC 2 cut(s) 3, 105
ApaLI GTGCAC 1 cut(s) 251
ApeKI GCWGC 2 cut(s) 35, 56
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AsuC2I CCSGG 2 cut(s) 19, 131
AsuHPI GGTGA 1 cut(s) 167
AsuII TTCGAA 1 cut(s) 448
BaeGI GKGCMC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 22, 68
BceAI ACGGC 4 cut(s) 167, 177, 510, 529
BcnI CCSGG 2 cut(s) 19, 131
BcoDI GTCTC 1 cut(s) 45
BcuI ACTAGT 1 cut(s) 244
BfaI CTAG 1 cut(s) 245
BisI GCNGC 3 cut(s) 36, 57, 279
BlsI GCNGC 3 cut(s) 37, 58, 280
Bme1390I CCNGG 2 cut(s) 19, 131
BmrFI CCNGG 2 cut(s) 19, 131
BmsI GCATC 2 cut(s) 20, 409
BpiI GAAGAC 1 cut(s) 527
Bpu14I TTCGAA 1 cut(s) 448
BpuMI CCSGG 2 cut(s) 19, 131
BsaJI CCNNGG 3 cut(s) 42, 108, 312
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 340
BseDI CCNNGG 3 cut(s) 42, 108, 312
BseLI CCNNNNNNNGG 2 cut(s) 130, 340
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 2 cut(s) 22, 68
BshFI GGCC 2 cut(s) 5, 107
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 2 cut(s) 19, 131
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 130, 340
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 2 cut(s) 5, 107
Bsp119I TTCGAA 1 cut(s) 448
Bsp1286I GDGCHC 2 cut(s) 93, 255
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 2 cut(s) 279, 309
BspANI GGCC 2 cut(s) 5, 107
BspT104I TTCGAA 1 cut(s) 448
BssECI CCNNGG 3 cut(s) 42, 108, 312
BssT1I CCWWGG 3 cut(s) 42, 108, 312
Bst4CI ACNGT 6 cut(s) 287, 380, 411, 507, 530, 571
BstBI TTCGAA 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 93, 463
BstDSI CCRYGG 1 cut(s) 108
BstMAI GTCTC 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 113, 287
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 2 cut(s) 17, 129
BstSLI GKGCMC 1 cut(s) 255
BstV1I GCAGC 2 cut(s) 22, 68
BstV2I GAAGAC 1 cut(s) 527
BsuRI GGCC 2 cut(s) 5, 107
BtgI CCRYGG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 93, 463
CseI GACGC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 405
CviAII CATG 2 cut(s) 109, 299
CviQI GTAC 1 cut(s) 405
Eco130I CCWWGG 3 cut(s) 42, 108, 312
Eco24I GRGCYC 1 cut(s) 93
EcoT14I CCWWGG 3 cut(s) 42, 108, 312
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 3 cut(s) 42, 108, 312
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 2 cut(s) 112, 302
FaiI YATR 5 cut(s) 110, 144, 300, 417, 444
FaqI GGGAC 1 cut(s) 60
FatI CATG 2 cut(s) 108, 298
Fnu4HI GCNGC 3 cut(s) 36, 57, 279
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 3 cut(s) 36, 57, 279
FspBI CTAG 1 cut(s) 245
GluI GCNGC 3 cut(s) 36, 57, 279
HaeIII GGCC 2 cut(s) 5, 107
HapII CCGG 2 cut(s) 19, 131
HgaI GACGC 1 cut(s) 62
Hin1II CATG 2 cut(s) 112, 302
HinfI GANTC 3 cut(s) 172, 206, 364
HpaII CCGG 2 cut(s) 19, 131
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 2 cut(s) 253, 407
Hpy188I TCNGA 6 cut(s) 81, 190, 328, 394, 489, 564
Hpy188III TCNNGA 1 cut(s) 29
Hpy8I GTNNAC 2 cut(s) 253, 407
Hpy99I CGWCG 3 cut(s) 287, 372, 398
HpyCH4III ACNGT 6 cut(s) 287, 380, 411, 507, 530, 571
HpyCH4V TGCA 3 cut(s) 11, 253, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 287
Hsp92II CATG 2 cut(s) 112, 302
LpnPI CCDG 2 cut(s) 32, 144
Lsp1109I GCAGC 2 cut(s) 22, 68
LweI GCATC 2 cut(s) 20, 409
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 2 cut(s) 203, 374
MboII GAAGA 1 cut(s) 532
MhlI GDGCHC 2 cut(s) 93, 255
MluCI AATT 3 cut(s) 151, 236, 476
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 3 cut(s) 168, 351, 417
MnlI CCTC 4 cut(s) 16, 25, 97, 337
MseI TTAA 2 cut(s) 219, 423
MspI CCGG 2 cut(s) 19, 131
MspR9I CCNGG 2 cut(s) 19, 131
MwoI GCNNNNNNNGC 2 cut(s) 113, 287
NciI CCSGG 2 cut(s) 19, 131
NcoI CCATGG 1 cut(s) 108
NlaIII CATG 2 cut(s) 112, 302
NmuCI GTSAC 1 cut(s) 203
NspI RCATGY 1 cut(s) 302
NspV TTCGAA 1 cut(s) 448
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PfeI GAWTC 2 cut(s) 172, 364
PfoI TCCNGGA 1 cut(s) 17
PkrI GCNGC 3 cut(s) 37, 58, 280
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
SaqAI TTAA 2 cut(s) 219, 423
SatI GCNGC 3 cut(s) 36, 57, 279
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 19, 131
SduI GDGCHC 2 cut(s) 93, 255
SetI ASST 5 cut(s) 79, 118, 272, 422, 467
SfaNI GCATC 2 cut(s) 20, 409
SfuI TTCGAA 1 cut(s) 448
SpeI ACTAGT 1 cut(s) 244
Sse9I AATT 3 cut(s) 151, 236, 476
SsiI CCGC 2 cut(s) 279, 309
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 2 cut(s) 17, 129
StyI CCWWGG 3 cut(s) 42, 108, 312
TaaI ACNGT 6 cut(s) 287, 380, 411, 507, 530, 571
TaqI TCGA 4 cut(s) 147, 294, 448, 551
TasI AATT 3 cut(s) 151, 236, 476
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 2 cut(s) 172, 364
Tru1I TTAA 2 cut(s) 219, 423
Tru9I TTAA 2 cut(s) 219, 423
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 2 cut(s) 35, 56
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 3 cut(s) 145, 404, 569
VneI GTGCAC 1 cut(s) 251
XceI RCATGY 1 cut(s) 302
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.