MD07G1047000.v1.1

DNAJ heat shock N-terminal domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001405
Physical Location & Seq
Forward (+)
4028018 .. 4028599
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1047000.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCTGCCGTCAAGGCCCTAGAGGCTGCAGAAGCATATTTCATGCTTGGAAACTTCGAATATGCAATCAAGCAGGCCAAGGCAGCAAAAGAGTTCGATCCAGATTTGCATGGTATTGACAGCTACGTGACCGCATACCAAATTCATTCTGCCGTGTCACACAAGCAAAACCTGTACCATGTTCTTGGCATCGAAAACCCTACAGTTGCCGACTCAGAAATCCTCAAGAAACAATACAAGAGACTGGCACTAGCCTTGCACCCTGACAAGAATAGATCAAACGCCGCGGAGGGTGCTTTCAAGCATATTAAGGCGGCATGGGACGTTCTCTCAGACCCTGCTAAGAAAGATGCTTACGACAAGTGTTTGACAAGAAGGCTTCAAGCGGCTTATGGTTCCAACAAACGCAGAGCTAGTGAGTGCAAACCGCCACCGCCGCATAGGTCCAGTGCCTTCCCTAGCAAAAGGTCTAGCCCTGGTGGTGATGGAAGCTGCTACAAGAAAACCATTAAGATTATTAGAAAATCAAATCCAGGCCAAAAGGCAACTGTGATGATGGTATCCGTACGCCGAGTGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

194

Amino Acids

21.33

Weight (kDa)

9.76

Isoelectric Point (pI)

59.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 57 - 120 7.1e-19 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 287
AciI CCGC 8 cut(s) 132, 285, 287, 314, 386, 428, 434, 437
AclWI GGATC 1 cut(s) 92
AcsI RAATTY 1 cut(s) 141
AfaI GTAC 2 cut(s) 176, 567
AgsI TTSAA 2 cut(s) 301, 383
AjnI CCWGG 2 cut(s) 475, 532
AluBI AGCT 3 cut(s) 123, 413, 492
AluI AGCT 3 cut(s) 123, 413, 492
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 1 cut(s) 92
AlwNI CAGNNNCTG 1 cut(s) 338
AoxI GGCC 3 cut(s) 15, 75, 535
ApeKI GCWGC 4 cut(s) 5, 26, 83, 492
ApoI RAATTY 1 cut(s) 141
AspS9I GGNCC 2 cut(s) 16, 444
AsuHPI GGTGA 1 cut(s) 494
AsuII TTCGAA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 444
BbvI GCAGC 3 cut(s) 13, 95, 479
BccI CCATC 2 cut(s) 479, 550
BceAI ACGGC 1 cut(s) 137
BciT130I CCWGG 2 cut(s) 477, 534
BciVI GTATCC 1 cut(s) 571
BcoDI GTCTC 1 cut(s) 235
BfaI CTAG 5 cut(s) 20, 251, 414, 459, 471
BfmI CTRYAG 2 cut(s) 27, 201
BfuI GTATCC 1 cut(s) 571
BglI GCCNNNNNGGC 2 cut(s) 14, 23
BisI GCNGC 8 cut(s) 6, 27, 84, 285, 315, 387, 437, 493
BlsI GCNGC 8 cut(s) 7, 28, 85, 286, 316, 388, 438, 494
Bme1390I CCNGG 2 cut(s) 477, 534
Bme18I GGWCC 1 cut(s) 444
BmgT120I GGNCC 2 cut(s) 16, 444
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 2 cut(s) 477, 534
BmsI GCATC 2 cut(s) 198, 340
Bpu14I TTCGAA 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 209
BsaAI YACGTR 1 cut(s) 127
BsaJI CCNNGG 3 cut(s) 78, 285, 475
Bse1I ACTGG 2 cut(s) 249, 447
BseBI CCWGG 2 cut(s) 477, 534
BseDI CCNNGG 3 cut(s) 78, 285, 475
BseMII CTCAG 2 cut(s) 228, 345
BseNI ACTGG 2 cut(s) 249, 447
BseXI GCAGC 3 cut(s) 13, 95, 479
Bsh1236I CGCG 1 cut(s) 287
BshFI GGCC 3 cut(s) 17, 77, 537
BsiWI CGTACG 1 cut(s) 565
BslFI GGGAC 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 335
BsnI GGCC 3 cut(s) 17, 77, 537
Bsp119I TTCGAA 1 cut(s) 57
Bsp143I GATC 2 cut(s) 97, 275
BspACI CCGC 8 cut(s) 132, 285, 287, 314, 386, 428, 434, 437
BspANI GGCC 3 cut(s) 17, 77, 537
BspCNI CTCAG 2 cut(s) 227, 344
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 31
BspPI GGATC 1 cut(s) 92
BspT104I TTCGAA 1 cut(s) 57
BsrI ACTGG 2 cut(s) 249, 447
BssECI CCNNGG 3 cut(s) 78, 285, 475
BssMI GATC 2 cut(s) 97, 275
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 2 cut(s) 477, 534
Bst4CI ACNGT 2 cut(s) 205, 550
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 3 cut(s) 214, 331, 342
BstDSI CCRYGG 1 cut(s) 285
BstFNI CGCG 1 cut(s) 287
BstKTI GATC 2 cut(s) 100, 278
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 2 cut(s) 97, 275
BstMWI GCNNNNNNNGC 6 cut(s) 14, 23, 32, 83, 293, 436
BstNI CCWGG 2 cut(s) 477, 534
BstSCI CCNGG 2 cut(s) 475, 532
BstSFI CTRYAG 2 cut(s) 27, 201
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 3 cut(s) 13, 95, 479
BstXI CCANNNNNNTGG 1 cut(s) 185
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 3 cut(s) 17, 77, 537
BtgI CCRYGG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 1 cut(s) 75
CaiI CAGNNNCTG 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 16, 444
Cfr42I CCGCGG 1 cut(s) 288
Csp6I GTAC 2 cut(s) 175, 566
CviAII CATG 4 cut(s) 43, 110, 179, 318
CviQI GTAC 2 cut(s) 175, 566
DdeI CTNAG 3 cut(s) 214, 331, 342
DpnI GATC 2 cut(s) 99, 277
DpnII GATC 2 cut(s) 97, 275
Eco130I CCWWGG 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 475, 532
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 4 cut(s) 46, 113, 182, 321
FaqI GGGAC 1 cut(s) 335
FatI CATG 4 cut(s) 42, 109, 178, 317
Fnu4HI GCNGC 8 cut(s) 6, 27, 84, 285, 315, 387, 437, 493
Fsp4HI GCNGC 8 cut(s) 6, 27, 84, 285, 315, 387, 437, 493
FspBI CTAG 5 cut(s) 20, 251, 414, 459, 471
GluI GCNGC 8 cut(s) 6, 27, 84, 285, 315, 387, 437, 493
HaeIII GGCC 3 cut(s) 17, 77, 537
Hin1II CATG 4 cut(s) 46, 113, 182, 321
HinfI GANTC 1 cut(s) 212
HphI GGTGA 1 cut(s) 494
Hpy188I TCNGA 2 cut(s) 217, 334
Hpy188III TCNNGA 2 cut(s) 101, 226
HpyAV CCTTC 2 cut(s) 369, 463
HpyCH4III ACNGT 2 cut(s) 205, 550
HpyCH4IV ACGT 2 cut(s) 126, 324
HpyCH4V TGCA 5 cut(s) 29, 65, 109, 259, 423
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 23, 32, 83, 293, 436
HpyF3I CTNAG 3 cut(s) 214, 331, 342
HpySE526I ACGT 2 cut(s) 126, 324
Hsp92II CATG 4 cut(s) 46, 113, 182, 321
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 2 cut(s) 97, 275
Lsp1109I GCAGC 3 cut(s) 13, 95, 479
LweI GCATC 2 cut(s) 198, 340
MaeI CTAG 5 cut(s) 20, 251, 414, 459, 471
MaeII ACGT 2 cut(s) 126, 324
MaeIII GTNAC 2 cut(s) 127, 156
MalI GATC 2 cut(s) 99, 277
MboI GATC 2 cut(s) 97, 275
MluCI AATT 1 cut(s) 141
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 3 cut(s) 16, 233, 283
MseI TTAA 3 cut(s) 309, 510, 580
MspA1I CMGCKG 1 cut(s) 287
MspR9I CCNGG 2 cut(s) 477, 534
MvaI CCWGG 2 cut(s) 477, 534
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 6 cut(s) 14, 23, 32, 83, 293, 436
NdeII GATC 2 cut(s) 97, 275
NlaIII CATG 4 cut(s) 46, 113, 182, 321
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 2 cut(s) 127, 156
NspV TTCGAA 1 cut(s) 57
Pfl23II CGTACG 1 cut(s) 565
PkrI GCNGC 8 cut(s) 7, 28, 85, 286, 316, 388, 438, 494
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
Ppu21I YACGTR 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 475, 532
PspGI CCWGG 2 cut(s) 475, 532
PspLI CGTACG 1 cut(s) 565
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 2 cut(s) 16, 444
PstI CTGCAG 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 338
RsaI GTAC 2 cut(s) 176, 567
RsaNI GTAC 2 cut(s) 175, 566
SacII CCGCGG 1 cut(s) 288
SaqAI TTAA 3 cut(s) 309, 510, 580
SatI GCNGC 8 cut(s) 6, 27, 84, 285, 315, 387, 437, 493
Sau3AI GATC 2 cut(s) 97, 275
Sau96I GGNCC 2 cut(s) 16, 444
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 2 cut(s) 477, 534
SetI ASST 8 cut(s) 125, 129, 174, 327, 415, 446, 470, 494
SfaNI GCATC 2 cut(s) 198, 340
SfcI CTRYAG 2 cut(s) 27, 201
Sfr303I CCGCGG 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 57
SgrBI CCGCGG 1 cut(s) 288
SinI GGWCC 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 1 cut(s) 141
SsiI CCGC 8 cut(s) 132, 285, 287, 314, 386, 428, 434, 437
SspMI CTAG 5 cut(s) 20, 251, 414, 459, 471
StyD4I CCNGG 2 cut(s) 475, 532
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 2 cut(s) 205, 550
TaiI ACGT 2 cut(s) 129, 327
TaqI TCGA 3 cut(s) 57, 96, 192
TasI AATT 1 cut(s) 141
TauI GCSGC 4 cut(s) 287, 317, 389, 439
Tru1I TTAA 3 cut(s) 309, 510, 580
Tru9I TTAA 3 cut(s) 309, 510, 580
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 2 cut(s) 127, 156
TseI GCWGC 4 cut(s) 5, 26, 83, 492
Tsp45I GTSAC 2 cut(s) 127, 156
TspDTI ATGAA 2 cut(s) 31, 134
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 1 cut(s) 454
VpaK11BI GGWCC 1 cut(s) 444
XapI RAATTY 1 cut(s) 141
XspI CTAG 5 cut(s) 20, 251, 414, 459, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.