FvH4_7g04790

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001298
Physical Location & Seq
Forward (+)
5226904 .. 5227935
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04790.t1

Sequence Viewer

Length: 1032 bp
ATGGGTGAGTTGGGTGAATGGGTTTTAGGAGATAGGATTGGGGAGGGTGGTTTCGGGTGTGTGTATAAGGGTTACCCACTCAAGCTTGAGAATGGGGCCCCTGGTATCATGGCCGTTAAGGTTGCAGAGGCCTCTCATCCATTCAAGGTTGATGCTTTGTTGGATGAGGCCGAGCTCCTTTCTCTATTTCACCAATGCCCTTTTGTGATCAATTCTTACGGTCAACAGTGGACTTCTTCCTCTCTCAATTTGTTTTTAGAATTTGCCGCTGGAGGCACCATTAGAGATAGGATTGACAATTCTGCATTGTCCCAATGTGAGATTAAGGAGTTCACCAAGTCTGTGCTTAGTGGTCTCCAGTGCATTCATGACAAAGGTTATGTGCATTGCGACATAAAGCCCGACAATATTCTTCTTGTCCATGATATGGATTCTCCTGTCGATTTCGTGGCTAAGATAGGAGACTTTGGATTAACTAAGAGTGCTCAGAACTTGCATTCTCGCCAAGGCACTAAATGGTATTTGCCTCCTGAAACTGTTCTCTGTAACATTCAGGGGAAACCATCTGATATTTGGGCAGTAGGATGTCTAGTTCTAGAGATGTTGACTGGTTCGTTGTGGACTTGTAAGTCAACTGAGTACCTAGGCGTTTGGGCTTCTAATCCAGTTATTCCTTCACACTTGTCTGATGACGCCAAGGATTTTTTGGCAAAGTGTCTAGATGTAGATGCTTACAAGAGATTTACGGCTCGGCGTCTCTTAGCTCACCCATTTCTTATGAGTAATCAGAGTGTTGAACAATTACCCAAATCGCTTACATTGTCACAATCTCAGCGTTGTCATAATCGGTTGCCTAGTTTTATACCCTTAGGGGACTCTAGCTCCCATGAGGCACAAATGCCCATTGAGAGTTTTGGATTAGATGATGATACCATTTTCCCTTTGGCACTCATGTCAAGACCTGCAAGACATGTGGTCGCAGACAAATGGTGCAAAAAGCCAACAACTTTTGCTGTAATGGGCGCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

344

Amino Acids

37.81

Weight (kDa)

5.76

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 9 - 254 3.5e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 10 - 259 2.2e-49 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 628
Acc36I ACCTGC 1 cut(s) 970
AccB1I GGYRCC 1 cut(s) 275
AccB7I CCANNNNNTGG 1 cut(s) 427
AciI CCGC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 1 cut(s) 260
AcyI GRCGYC 2 cut(s) 693, 754
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 1 cut(s) 427
AflIII ACRYGT 1 cut(s) 970
AgsI TTSAA 2 cut(s) 145, 797
AhdI GACNNNNNGTC 1 cut(s) 974
AjnI CCWGG 1 cut(s) 100
AloI GAACNNNNNNTCC 2 cut(s) 576, 608
AluBI AGCT 4 cut(s) 85, 175, 764, 882
AluI AGCT 4 cut(s) 85, 175, 764, 882
Alw21I GWGCWC 2 cut(s) 177, 487
Alw26I GTCTC 3 cut(s) 359, 456, 761
AoxI GGCC 4 cut(s) 96, 111, 129, 168
ApaI GGGCCC 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 1025
ApoI RAATTY 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 537
AspA2I CCTAGG 1 cut(s) 643
AspLEI GCGC 1 cut(s) 1025
AspS9I GGNCC 2 cut(s) 96, 97
AsuHPI GGTGA 5 cut(s) 17, 26, 182, 325, 758
AvrII CCTAGG 1 cut(s) 643
AxyI CCTNAGG 1 cut(s) 868
BaeGI GKGCMC 1 cut(s) 100
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 100, 177
Bbv12I GWGCWC 2 cut(s) 177, 487
BbvI GCAGC 1 cut(s) 1012
BccI CCATC 1 cut(s) 571
BceAI ACGGC 2 cut(s) 98, 762
BciT130I CCWGG 1 cut(s) 102
BclI TGATCA 1 cut(s) 207
BcoDI GTCTC 3 cut(s) 359, 456, 761
BfaI CTAG 6 cut(s) 590, 596, 644, 719, 855, 879
BfoI RGCGCY 1 cut(s) 1026
BfuAI ACCTGC 1 cut(s) 970
BisI GCNGC 2 cut(s) 267, 1026
BlnI CCTAGG 1 cut(s) 643
BlsI GCNGC 2 cut(s) 268, 1027
Bme1390I CCNGG 1 cut(s) 102
BmeRI GACNNNNNGTC 1 cut(s) 974
BmgT120I GGNCC 2 cut(s) 96, 97
BmiI GGNNCC 4 cut(s) 97, 98, 99, 277
BmrFI CCNGG 1 cut(s) 102
BmsI GCATC 2 cut(s) 142, 718
BpmI CTGGAG 2 cut(s) 291, 341
BpuEI CTTGAG 2 cut(s) 65, 107
BsaHI GRCGYC 2 cut(s) 693, 754
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 4 cut(s) 100, 505, 643, 696
BsaXI ACNNNNNCTCC 2 cut(s) 866, 896
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 3 cut(s) 358, 613, 665
Bse21I CCTNAGG 1 cut(s) 868
Bse3DI GCAATG 1 cut(s) 385
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 4 cut(s) 100, 505, 643, 696
BseGI GGATG 3 cut(s) 136, 169, 590
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 3 cut(s) 500, 627, 845
BseNI ACTGG 3 cut(s) 358, 613, 665
BseSI GKGCMC 1 cut(s) 100
BseXI GCAGC 1 cut(s) 1012
BshFI GGCC 4 cut(s) 98, 113, 131, 170
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 2 cut(s) 177, 487
BslFI GGGAC 2 cut(s) 295, 887
BslI CCNNNNNNNGG 1 cut(s) 427
BsmAI GTCTC 3 cut(s) 359, 456, 761
BsmBI CGTCTC 1 cut(s) 761
BsmFI GGGAC 2 cut(s) 295, 887
BsmI GAATGC 2 cut(s) 363, 496
BsnI GGCC 4 cut(s) 98, 113, 131, 170
Bso31I GGTCTC 1 cut(s) 359
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 3 cut(s) 100, 177, 487
Bsp143I GATC 1 cut(s) 207
BspACI CCGC 1 cut(s) 267
BspANI GGCC 4 cut(s) 98, 113, 131, 170
BspCNI CTCAG 3 cut(s) 499, 628, 844
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 4 cut(s) 97, 98, 99, 277
BspMI ACCTGC 1 cut(s) 970
BspT107I GGYRCC 1 cut(s) 275
BspTNI GGTCTC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 3 cut(s) 358, 613, 665
BssECI CCNNGG 4 cut(s) 100, 505, 643, 696
BssMI GATC 1 cut(s) 207
BssNI GRCGYC 2 cut(s) 693, 754
BssT1I CCWWGG 3 cut(s) 505, 643, 696
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 3 cut(s) 221, 228, 538
BstACI GRCGYC 2 cut(s) 693, 754
BstDEI CTNAG 8 cut(s) 347, 453, 477, 486, 636, 760, 831, 868
BstEII GGTNACC 1 cut(s) 71
BstF5I GGATG 3 cut(s) 136, 169, 590
BstH2I RGCGCY 1 cut(s) 1026
BstHHI GCGC 1 cut(s) 1025
BstKTI GATC 1 cut(s) 210
BstMAI GTCTC 3 cut(s) 359, 456, 761
BstMBI GATC 1 cut(s) 207
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 1 cut(s) 974
BstPI GGTNACC 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 100
BstSLI GKGCMC 1 cut(s) 100
BstV1I GCAGC 1 cut(s) 1012
Bsu36I CCTNAGG 1 cut(s) 868
BsuRI GGCC 4 cut(s) 98, 113, 131, 170
BtsCI GGATG 3 cut(s) 136, 169, 590
BtsIMutI CAGTG 2 cut(s) 233, 365
BveI ACCTGC 1 cut(s) 970
CciI TCATGA 1 cut(s) 367
CfoI GCGC 1 cut(s) 1025
Cfr13I GGNCC 2 cut(s) 96, 97
CseI GACGC 2 cut(s) 701, 743
Csp6I GTAC 1 cut(s) 640
CspCI CAANNNNNGTGG 2 cut(s) 954, 989
CviAII CATG 6 cut(s) 109, 368, 422, 887, 952, 971
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 8 cut(s) 347, 453, 477, 486, 636, 760, 831, 868
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
DrdI GACNNNNNNGTC 1 cut(s) 628
DriI GACNNNNNGTC 1 cut(s) 974
DseDI GACNNNNNNGTC 1 cut(s) 628
EaeI YGGCCR 1 cut(s) 111
Eam1105I GACNNNNNGTC 1 cut(s) 974
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 3 cut(s) 505, 643, 696
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 2 cut(s) 100, 177
Eco31I GGTCTC 1 cut(s) 359
Eco53kI GAGCTC 1 cut(s) 175
Eco81I CCTNAGG 1 cut(s) 868
Eco91I GGTNACC 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 175
EcoO109I RGGNCCY 2 cut(s) 96, 97
EcoO65I GGTNACC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 3 cut(s) 505, 643, 696
EcoT38I GRGCYC 2 cut(s) 100, 177
ErhI CCWWGG 3 cut(s) 505, 643, 696
Esp3I CGTCTC 1 cut(s) 761
FaeI CATG 6 cut(s) 112, 371, 425, 890, 955, 974
FaqI GGGAC 2 cut(s) 295, 887
FatI CATG 6 cut(s) 108, 367, 421, 886, 951, 970
FbaI TGATCA 1 cut(s) 207
Fnu4HI GCNGC 2 cut(s) 267, 1026
FokI GGATG 3 cut(s) 123, 176, 597
FriOI GRGCYC 2 cut(s) 100, 177
Fsp4HI GCNGC 2 cut(s) 267, 1026
FspBI CTAG 6 cut(s) 590, 596, 644, 719, 855, 879
GlaI GCGC 1 cut(s) 1024
GluI GCNGC 2 cut(s) 267, 1026
GsuI CTGGAG 2 cut(s) 291, 341
HaeII RGCGCY 1 cut(s) 1026
HaeIII GGCC 4 cut(s) 98, 113, 131, 170
HgaI GACGC 2 cut(s) 701, 743
HhaI GCGC 1 cut(s) 1025
Hin1I GRCGYC 2 cut(s) 693, 754
Hin1II CATG 6 cut(s) 112, 371, 425, 890, 955, 974
Hin6I GCGC 1 cut(s) 1023
HinP1I GCGC 1 cut(s) 1023
HincII GTYRAC 3 cut(s) 224, 606, 633
HindII GTYRAC 3 cut(s) 224, 606, 633
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 2 cut(s) 431, 875
HphI GGTGA 5 cut(s) 17, 26, 182, 325, 758
Hpy166II GTNNAC 6 cut(s) 224, 231, 333, 606, 621, 633
Hpy188I TCNGA 4 cut(s) 489, 568, 688, 789
Hpy188III TCNNGA 5 cut(s) 368, 530, 596, 719, 957
Hpy8I GTNNAC 6 cut(s) 224, 231, 333, 606, 621, 633
HpyAV CCTTC 1 cut(s) 684
HpyCH4III ACNGT 3 cut(s) 221, 228, 538
HpyCH4V TGCA 8 cut(s) 125, 305, 363, 385, 496, 965, 993, 1028
HpyF3I CTNAG 8 cut(s) 347, 453, 477, 486, 636, 760, 831, 868
Hsp92I GRCGYC 2 cut(s) 693, 754
Hsp92II CATG 6 cut(s) 112, 371, 425, 890, 955, 974
HspAI GCGC 1 cut(s) 1023
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 1 cut(s) 207
LmnI GCTCC 2 cut(s) 180, 887
Lsp1109I GCAGC 1 cut(s) 1012
LweI GCATC 2 cut(s) 142, 718
MaeI CTAG 6 cut(s) 590, 596, 644, 719, 855, 879
MaeIII GTNAC 3 cut(s) 71, 545, 822
MalI GATC 1 cut(s) 209
MboI GATC 1 cut(s) 207
MboII GAAGA 2 cut(s) 228, 404
MhlI GDGCHC 3 cut(s) 100, 177, 487
MluCI AATT 5 cut(s) 211, 247, 260, 298, 800
MlyI GAGTC 1 cut(s) 869
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 8 cut(s) 37, 121, 142, 160, 250, 266, 537, 883
MroXI GAANNNNTTC 1 cut(s) 537
MseI TTAA 3 cut(s) 117, 324, 473
MspA1I CMGCKG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 102
Mva1269I GAATGC 2 cut(s) 363, 496
MvaI CCWGG 1 cut(s) 102
NdeII GATC 1 cut(s) 207
NlaIII CATG 6 cut(s) 112, 371, 425, 890, 955, 974
NlaIV GGNNCC 4 cut(s) 97, 98, 99, 277
NmeAIII GCCGAG 2 cut(s) 196, 730
NmuCI GTSAC 1 cut(s) 822
NspI RCATGY 1 cut(s) 974
PagI TCATGA 1 cut(s) 367
PceI AGGCCT 1 cut(s) 131
PciI ACATGT 1 cut(s) 970
PctI GAATGC 2 cut(s) 363, 496
PdmI GAANNNNTTC 1 cut(s) 537
PfeI GAWTC 1 cut(s) 431
PflMI CCANNNNNTGG 1 cut(s) 427
PkrI GCNGC 2 cut(s) 268, 1027
PleI GAGTC 1 cut(s) 869
PpsI GAGTC 1 cut(s) 869
PscI ACATGT 1 cut(s) 970
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 100
PspEI GGTNACC 1 cut(s) 71
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 4 cut(s) 97, 98, 99, 277
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 2 cut(s) 96, 97
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 3 cut(s) 117, 324, 473
SatI GCNGC 2 cut(s) 267, 1026
Sau3AI GATC 1 cut(s) 207
Sau96I GGNCC 2 cut(s) 96, 97
SchI GAGTC 1 cut(s) 869
ScrFI CCNGG 1 cut(s) 102
SduI GDGCHC 3 cut(s) 100, 177, 487
SetI ASST 9 cut(s) 87, 123, 150, 177, 379, 645, 766, 884, 964
SfaNI GCATC 2 cut(s) 142, 718
SmlI CTYRAG 2 cut(s) 80, 86
SmoI CTYRAG 2 cut(s) 80, 86
Sse9I AATT 5 cut(s) 211, 247, 260, 298, 800
SseBI AGGCCT 1 cut(s) 131
SsiI CCGC 1 cut(s) 267
SspI AATATT 1 cut(s) 409
SspMI CTAG 6 cut(s) 590, 596, 644, 719, 855, 879
SstI GAGCTC 1 cut(s) 177
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 3 cut(s) 505, 643, 696
TaaI ACNGT 3 cut(s) 221, 228, 538
TaqI TCGA 1 cut(s) 441
TasI AATT 5 cut(s) 211, 247, 260, 298, 800
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 3 cut(s) 117, 324, 473
Tru9I TTAA 3 cut(s) 117, 324, 473
TscAI CASTG 2 cut(s) 233, 365
TseFI GTSAC 1 cut(s) 822
TseI GCWGC 1 cut(s) 1025
Tsp45I GTSAC 1 cut(s) 822
TspDTI ATGAA 1 cut(s) 356
TspRI CASTG 2 cut(s) 233, 365
Van91I CCANNNNNTGG 1 cut(s) 427
XapI RAATTY 1 cut(s) 260
XbaI TCTAGA 2 cut(s) 595, 718
XceI RCATGY 1 cut(s) 974
XcmI CCANNNNNNNNNTGG 3 cut(s) 570, 703, 940
XmaJI CCTAGG 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 537
XspI CTAG 6 cut(s) 590, 596, 644, 719, 855, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.