Prupe.5G038600_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001298
Physical Location & Seq
Forward (+)
4332075 .. 4333577
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G038600.1

Sequence Viewer

Length: 1131 bp
ATGGCTTCATTGAAGCAGAAAAGAGAAACCCTAAAGGAAGACGAACCATGGCTTGTAGAGAAGAGTTTTTTAGTTGCTGGGCGTGAGTGGGTTCGAGGGCGGATTCTCGGCCAAGGCGGATTTGGGTCGGTTTATTTGGCCTCTGTGAAAAAACCCAAATTGGGTAGGGAGGGTTTTCCGGCGATCATGGCTGTGAAATCGGCATTGATGGCTAAATCGTTTGAGCTGGTGAGCGAAAGGTCACTTCTTGGCGAGTTTCGTGGGTGCCCTTTTGTTATCGACTGTTATGGAGAAGATTTCACGGCTGGGATTGGATATAATTTGGTATACAATGTGTTCTTGGAATATGCTGATGGAGGAACAATAGGGGATTTGATAAAGCAATCTGGGGGCTCCGGGCTCTGTGAATTACAAGTGAGGAAGTATACAGAGTCGATTTTGAAAGGAATTCAGTACATTCACGAAAGGGGTTTTGTGCACTGTGATTTGAAGCCTGAAAACATCTTACTTGTGACCTCTGGTTCTGGTTTTGTGCCCAAAATTGGGGACTTGGGACTGGCAAAGAGGGCTGCGGGTGAGCGTTCTTGTAGAGGCACTGCAATGTACTTATCTCCTGAAACTGTGCTTGATAGCATTCAGGAGAAGCCTTCCGATATTTGGGCATTGGGCTGTGTTGTGCTCAAGATGCTCACTGGGAGGCACCCATGGGATGCAAAAGCCGGCTTGAAACTTCATGACCTAAAGCCTCTGATTGCTTCTGGAGTTCCTAAGGTTCCTGGTGGGTTATCAAAAGATGCTAGAGATTTCTTGAAGAATTGCTTTGCGAGGAACCCTTCACAGAGGCTTACAGCAGCTAAGCTATTGGAACACCCCTTTGTGACTAAAGTTGATGAGATTGCACAGGTTAAAGCTGAACCAATAAAGGAAGTTTCTTCAGTGTCATCTTCTTCGAATTGCAGTCTTGATTATGGTAGTTTCATACCACTTGGAAGTTGGAGCTCTGAAAATGCAGAGGAGATGGAGCAACAGATTCTTCCTTTGGCCATCTTGTACCCTAGAGATTCAAGGCCTGTGATCCCAAACACCGGGTGCCAAAAGCCATCAGCTTTTGCTATAATGGGTGCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

377

Amino Acids

40.93

Weight (kDa)

8.45

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 264, 699, 1089
AccI GTMKAC 2 cut(s) 327, 425
AciI CCGC 3 cut(s) 100, 117, 572
AclWI GGATC 1 cut(s) 1069
AcoI YGGCCR 2 cut(s) 109, 1041
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 918
AdeI CACNNNGTG 1 cut(s) 1089
AfaI GTAC 3 cut(s) 455, 605, 1052
AfiI CCNNNNNNNGG 5 cut(s) 161, 542, 543, 657, 1085
AgsI TTSAA 6 cut(s) 13, 442, 490, 727, 811, 1065
AjnI CCWGG 1 cut(s) 775
AluBI AGCT 7 cut(s) 226, 854, 859, 911, 999, 1106, 1127
AluI AGCT 7 cut(s) 226, 854, 859, 911, 999, 1106, 1127
Alw21I GWGCWC 3 cut(s) 480, 681, 1001
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 1 cut(s) 1069
AoxI GGCC 4 cut(s) 109, 138, 1041, 1067
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 3 cut(s) 569, 851, 1124
ApoI RAATTY 1 cut(s) 447
AsuC2I CCSGG 2 cut(s) 397, 1087
AsuHPI GGTGA 2 cut(s) 241, 587
AsuII TTCGAA 1 cut(s) 950
AxyI CCTNAGG 1 cut(s) 768
BaeGI GKGCMC 3 cut(s) 269, 480, 537
BalI TGGCCA 1 cut(s) 1043
BanI GGYRCC 3 cut(s) 264, 699, 1089
BanII GRGCYC 3 cut(s) 395, 402, 1001
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 3 cut(s) 480, 681, 1001
BbvI GCAGC 2 cut(s) 556, 863
BccI CCATC 5 cut(s) 202, 347, 1012, 1052, 1108
BceAI ACGGC 1 cut(s) 318
BciT130I CCWGG 1 cut(s) 777
BcnI CCSGG 2 cut(s) 397, 1087
BfaI CTAG 2 cut(s) 798, 1056
BisI GCNGC 3 cut(s) 570, 852, 1125
BlpI GCTNAGC 1 cut(s) 855
BlsI GCNGC 3 cut(s) 571, 853, 1126
Bme1390I CCNGG 3 cut(s) 397, 777, 1087
BmiI GGNNCC 6 cut(s) 266, 394, 701, 774, 830, 1091
BmrFI CCNGG 3 cut(s) 397, 777, 1087
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 3 cut(s) 675, 700, 784
BmuI ACTGGG 1 cut(s) 702
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 780
Bpu1102I GCTNAGC 1 cut(s) 855
Bpu14I TTCGAA 1 cut(s) 950
BpuEI CTTGAG 1 cut(s) 665
BpuMI CCSGG 2 cut(s) 397, 1087
BsaJI CCNNGG 3 cut(s) 47, 112, 704
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 542, 543, 657, 1085
Bse118I RCCGGY 1 cut(s) 719
Bse1I ACTGG 2 cut(s) 561, 697
Bse21I CCTNAGG 1 cut(s) 768
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 3 cut(s) 47, 112, 704
BseGI GGATG 1 cut(s) 715
BseLI CCNNNNNNNGG 5 cut(s) 161, 542, 543, 657, 1085
BseMI GCAATG 1 cut(s) 606
BseNI ACTGG 2 cut(s) 561, 697
BseRI GAGGAG 1 cut(s) 1028
BseSI GKGCMC 3 cut(s) 269, 480, 537
BseXI GCAGC 2 cut(s) 556, 863
BseYI CCCAGC 2 cut(s) 77, 305
BshFI GGCC 4 cut(s) 111, 140, 1043, 1069
BshNI GGYRCC 3 cut(s) 264, 699, 1089
BsiHKAI GWGCWC 3 cut(s) 480, 681, 1001
BsiSI CCGG 4 cut(s) 179, 396, 720, 1086
BslFI GGGAC 2 cut(s) 560, 567
BslI CCNNNNNNNGG 5 cut(s) 161, 542, 543, 657, 1085
BsmFI GGGAC 2 cut(s) 560, 567
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 4 cut(s) 111, 140, 1043, 1069
Bsp119I TTCGAA 1 cut(s) 950
Bsp1286I GDGCHC 7 cut(s) 269, 395, 402, 480, 537, 681, 1001
Bsp143I GATC 2 cut(s) 183, 1074
Bsp1720I GCTNAGC 1 cut(s) 855
Bsp19I CCATGG 2 cut(s) 47, 704
BspACI CCGC 3 cut(s) 100, 117, 572
BspANI GGCC 4 cut(s) 111, 140, 1043, 1069
BspHI TCATGA 1 cut(s) 733
BspLI GGNNCC 6 cut(s) 266, 394, 701, 774, 830, 1091
BspPI GGATC 1 cut(s) 1069
BspT104I TTCGAA 1 cut(s) 950
BspT107I GGYRCC 3 cut(s) 264, 699, 1089
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 719
BsrI ACTGG 2 cut(s) 561, 697
BssAI RCCGGY 1 cut(s) 719
BssECI CCNNGG 3 cut(s) 47, 112, 704
BssMI GATC 2 cut(s) 183, 1074
BssNAI GTATAC 2 cut(s) 328, 426
BssT1I CCWWGG 3 cut(s) 47, 112, 704
Bst1107I GTATAC 2 cut(s) 328, 426
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 3 cut(s) 284, 482, 622
Bst6I CTCTTC 1 cut(s) 56
BstBI TTCGAA 1 cut(s) 950
BstC8I GCNNGC 1 cut(s) 721
BstDEI CTNAG 3 cut(s) 768, 855, 1128
BstDSI CCRYGG 2 cut(s) 47, 704
BstF5I GGATG 1 cut(s) 715
BstKTI GATC 2 cut(s) 186, 1077
BstMBI GATC 2 cut(s) 183, 1074
BstMWI GCNNNNNNNGC 4 cut(s) 188, 209, 566, 685
BstNI CCWGG 1 cut(s) 777
BstSCI CCNGG 3 cut(s) 395, 775, 1085
BstSLI GKGCMC 3 cut(s) 269, 480, 537
BstV1I GCAGC 2 cut(s) 556, 863
BstV2I GAAGAC 1 cut(s) 45
BstZ17I GTATAC 2 cut(s) 328, 426
Bsu36I CCTNAGG 1 cut(s) 768
BsuRI GGCC 4 cut(s) 111, 140, 1043, 1069
BtgI CCRYGG 2 cut(s) 47, 704
BtsCI GGATG 1 cut(s) 715
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 4 cut(s) 478, 594, 690, 942
Cac8I GCNNGC 1 cut(s) 721
CciI TCATGA 1 cut(s) 733
Cfr10I RCCGGY 1 cut(s) 719
Csp6I GTAC 3 cut(s) 454, 604, 1051
CviAII CATG 4 cut(s) 48, 187, 705, 734
CviQI GTAC 3 cut(s) 454, 604, 1051
DdeI CTNAG 3 cut(s) 768, 855, 1128
DpnI GATC 2 cut(s) 185, 1076
DpnII GATC 2 cut(s) 183, 1074
DraIII CACNNNGTG 1 cut(s) 1089
EaeI YGGCCR 2 cut(s) 109, 1041
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 2 cut(s) 115, 132
Ecl136II GAGCTC 1 cut(s) 999
Eco130I CCWWGG 3 cut(s) 47, 112, 704
Eco147I AGGCCT 1 cut(s) 1069
Eco24I GRGCYC 3 cut(s) 395, 402, 1001
Eco53kI GAGCTC 1 cut(s) 999
Eco57I CTGAAG 1 cut(s) 918
Eco81I CCTNAGG 1 cut(s) 768
EcoICRI GAGCTC 1 cut(s) 999
EcoRI GAATTC 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 775
EcoT14I CCWWGG 3 cut(s) 47, 112, 704
EcoT38I GRGCYC 3 cut(s) 395, 402, 1001
ErhI CCWWGG 3 cut(s) 47, 112, 704
FaeI CATG 4 cut(s) 51, 190, 708, 737
FalI AAGNNNNNCTT 2 cut(s) 803, 835
FaqI GGGAC 2 cut(s) 560, 567
FatI CATG 4 cut(s) 47, 186, 704, 733
FauI CCCGC 1 cut(s) 565
FblI GTMKAC 2 cut(s) 327, 425
Fnu4HI GCNGC 3 cut(s) 570, 852, 1125
FokI GGATG 1 cut(s) 722
FriOI GRGCYC 3 cut(s) 395, 402, 1001
Fsp4HI GCNGC 3 cut(s) 570, 852, 1125
FspBI CTAG 2 cut(s) 798, 1056
GluI GCNGC 3 cut(s) 570, 852, 1125
GsaI CCCAGC 2 cut(s) 81, 309
GsuI CTGGAG 1 cut(s) 780
HaeIII GGCC 4 cut(s) 111, 140, 1043, 1069
HapII CCGG 4 cut(s) 179, 396, 720, 1086
Hin1II CATG 4 cut(s) 51, 190, 708, 737
HinfI GANTC 4 cut(s) 103, 431, 1030, 1061
HpaII CCGG 4 cut(s) 179, 396, 720, 1086
HphI GGTGA 2 cut(s) 241, 587
Hpy166II GTNNAC 3 cut(s) 328, 426, 478
Hpy188I TCNGA 3 cut(s) 652, 750, 1003
Hpy188III TCNNGA 8 cut(s) 461, 614, 638, 682, 734, 759, 808, 962
Hpy8I GTNNAC 3 cut(s) 328, 426, 478
HpyAV CCTTC 2 cut(s) 657, 843
HpyCH4III ACNGT 3 cut(s) 284, 482, 622
HpyCH4V TGCA 7 cut(s) 478, 599, 713, 899, 957, 1010, 1124
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 209, 566, 685
HpyF3I CTNAG 3 cut(s) 768, 855, 1128
Hsp92II CATG 4 cut(s) 51, 190, 708, 737
KroI GCCGGC 1 cut(s) 719
KroNI GCCGGC 1 cut(s) 721
Kzo9I GATC 2 cut(s) 183, 1074
LmnI GCTCC 3 cut(s) 398, 996, 1021
Lsp1109I GCAGC 2 cut(s) 556, 863
LweI GCATC 3 cut(s) 675, 700, 784
MaeI CTAG 2 cut(s) 798, 1056
MaeIII GTNAC 3 cut(s) 240, 511, 877
MalI GATC 2 cut(s) 185, 1076
MboI GATC 2 cut(s) 183, 1074
MboII GAAGA 8 cut(s) 50, 73, 305, 823, 924, 936, 939, 1025
MhlI GDGCHC 7 cut(s) 269, 395, 402, 480, 537, 681, 1001
MlsI TGGCCA 1 cut(s) 1043
MluCI AATT 7 cut(s) 158, 319, 407, 447, 540, 814, 952
MluNI TGGCCA 1 cut(s) 1043
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 974
Mox20I TGGCCA 1 cut(s) 1043
MroNI GCCGGC 1 cut(s) 719
MscI TGGCCA 1 cut(s) 1043
MseI TTAA 1 cut(s) 906
MslI CAYNNNNRTG 2 cut(s) 191, 599
Msp20I TGGCCA 1 cut(s) 1043
MspI CCGG 4 cut(s) 179, 396, 720, 1086
MspR9I CCNGG 3 cut(s) 397, 777, 1087
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 1 cut(s) 777
MwoI GCNNNNNNNGC 4 cut(s) 188, 209, 566, 685
NaeI GCCGGC 1 cut(s) 721
NciI CCSGG 2 cut(s) 397, 1087
NcoI CCATGG 2 cut(s) 47, 704
NdeII GATC 2 cut(s) 183, 1074
NgoMIV GCCGGC 1 cut(s) 719
NlaIII CATG 4 cut(s) 51, 190, 708, 737
NlaIV GGNNCC 6 cut(s) 266, 394, 701, 774, 830, 1091
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 3 cut(s) 240, 511, 877
NspV TTCGAA 1 cut(s) 950
PagI TCATGA 1 cut(s) 733
PceI AGGCCT 1 cut(s) 1069
PctI GAATGC 1 cut(s) 633
PdiI GCCGGC 1 cut(s) 721
PfeI GAWTC 3 cut(s) 103, 1030, 1061
PkrI GCNGC 3 cut(s) 571, 853, 1126
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
Psp124BI GAGCTC 1 cut(s) 1001
Psp6I CCWGG 1 cut(s) 775
PspFI CCCAGC 2 cut(s) 77, 305
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 6 cut(s) 266, 394, 701, 774, 830, 1091
RsaI GTAC 3 cut(s) 455, 605, 1052
RsaNI GTAC 3 cut(s) 454, 604, 1051
RseI CAYNNNNRTG 2 cut(s) 191, 599
SacI GAGCTC 1 cut(s) 1001
SaqAI TTAA 1 cut(s) 906
SatI GCNGC 3 cut(s) 570, 852, 1125
Sau3AI GATC 2 cut(s) 183, 1074
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 397, 777, 1087
SduI GDGCHC 7 cut(s) 269, 395, 402, 480, 537, 681, 1001
SfaNI GCATC 3 cut(s) 675, 700, 784
SfuI TTCGAA 1 cut(s) 950
SmiMI CAYNNNNRTG 2 cut(s) 191, 599
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 7 cut(s) 158, 319, 407, 447, 540, 814, 952
SseBI AGGCCT 1 cut(s) 1069
SsiI CCGC 3 cut(s) 100, 117, 572
SspMI CTAG 2 cut(s) 798, 1056
SstI GAGCTC 1 cut(s) 1001
StuI AGGCCT 1 cut(s) 1069
StyD4I CCNGG 3 cut(s) 395, 775, 1085
StyI CCWWGG 3 cut(s) 47, 112, 704
TaaI ACNGT 3 cut(s) 284, 482, 622
TaqI TCGA 4 cut(s) 94, 279, 434, 950
TasI AATT 7 cut(s) 158, 319, 407, 447, 540, 814, 952
TatI WGTACW 2 cut(s) 453, 603
TfiI GAWTC 3 cut(s) 103, 1030, 1061
Tru1I TTAA 1 cut(s) 906
Tru9I TTAA 1 cut(s) 906
TscAI CASTG 4 cut(s) 485, 601, 697, 942
TseFI GTSAC 3 cut(s) 240, 511, 877
TseI GCWGC 3 cut(s) 569, 851, 1124
Tsp45I GTSAC 3 cut(s) 240, 511, 877
TspDTI ATGAA 2 cut(s) 722, 967
TspRI CASTG 4 cut(s) 485, 601, 697, 942
VneI GTGCAC 1 cut(s) 476
XapI RAATTY 1 cut(s) 447
XcmI CCANNNNNNNNNTGG 2 cut(s) 119, 990
XmiI GTMKAC 2 cut(s) 327, 425
XspI CTAG 2 cut(s) 798, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.