Rroxscaffold_2G00098220

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001298
Physical Location & Seq
Reverse (-)
19406434 .. 19407255
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098220.1

Sequence Viewer

Length: 822 bp
ATGATTGCATCAGACTATTTCAAGGCCGTGGAAAAGCTCGAGAAGAAGAGGAAAAGAATCCGGGAGGAAGAGCCGGATTCTGGCAACGTCGGTCGGACCAAACGGTGGGTTCGACAAAGGTTCCTCGGCGCAGGACAATTCGGGACGGTTTTCCTGGCTAGTCCGGCCAAACCAGGTTCCAAACTGCCGCCGGTCATGGCCGTGAAGTCGGCGATGCTCTCGGAATCTGCCGACTTGGTTAAAGAAAGTCGACTTCTCAAGAAATTCAAGAACTGCCCTTTTATCCTTGACACTTACGGCTGCGATGTGACTAAAGATGTCGAAGGTGAGGAGTTGTTCAACGTTTTCTTGGAGTATGCTCAAAGAGGAACCGTAGAGGATTGGATTCAGAAACTCAAGAATTTCGGGTTGCATGAGCTGCACGTAAGGAGGTATACACAGTCACTATTGAAGGGGCTCAATTACATTCATGGAAAGGGTTTTGTGCACTGTGACATTAAGCCTGCCAATGTATTGCTAGTGCGTGACGGTCCTGATGATACCGGGTTTGGATCGTACGTGGCGAAAATCGGGGATTTAGGGCTGGCAAAGAGGGCTGACAAGTACAATGTGATGAAGCGTTCTGATTATATAAATGGCACTGAGTTGTATATGTCTCCAGAAGTATCGCTTCACAACATTCAGGAGCCGCCGGCCGACATTTGGGCATTAGGCTGCACTGTTCTGCAGATGTTAACTGGGATGGAGTTTTGGGATGTGAACGCTGACTTAAGAGATGACTGGGGGTACCGATTGAGATTCCGGTTATCCCTGAAACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

273

Amino Acids

31.17

Weight (kDa)

9.1

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 38 - 252 7.3e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 39 - 246 6.4e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 786
AccB1I GGYRCC 1 cut(s) 786
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 2 cut(s) 250, 434
AciI CCGC 2 cut(s) 188, 689
AclI AACGTT 1 cut(s) 342
AclWI GGATC 1 cut(s) 559
AcoI YGGCCR 3 cut(s) 165, 198, 693
AcsI RAATTY 2 cut(s) 263, 400
AfaI GTAC 3 cut(s) 557, 605, 788
AfiI CCNNNNNNNGG 3 cut(s) 80, 105, 702
AflII CTTAAG 1 cut(s) 769
AgsI TTSAA 4 cut(s) 22, 268, 340, 451
AjnI CCWGG 2 cut(s) 153, 172
AluBI AGCT 2 cut(s) 37, 418
AluI AGCT 2 cut(s) 37, 418
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 1 cut(s) 660
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 1 cut(s) 559
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 4 cut(s) 24, 165, 198, 693
ApaLI GTGCAC 1 cut(s) 485
ApeKI GCWGC 3 cut(s) 300, 418, 714
ApoI RAATTY 2 cut(s) 263, 400
Asp718I GGTACC 1 cut(s) 786
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 96, 530
AsuC2I CCSGG 2 cut(s) 62, 544
AsuHPI GGTGA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 2 cut(s) 96, 530
BaeGI GKGCMC 1 cut(s) 489
BanI GGYRCC 1 cut(s) 786
BanII GRGCYC 1 cut(s) 459
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 3 cut(s) 287, 405, 701
BccI CCATC 1 cut(s) 736
BceAI ACGGC 3 cut(s) 11, 185, 313
BciT130I CCWGG 2 cut(s) 155, 174
BcnI CCSGG 2 cut(s) 62, 544
BcoDI GTCTC 1 cut(s) 660
BfaI CTAG 2 cut(s) 159, 518
BfmI CTRYAG 1 cut(s) 725
BfrI CTTAAG 1 cut(s) 769
BisI GCNGC 5 cut(s) 188, 301, 419, 689, 715
BlsI GCNGC 5 cut(s) 189, 302, 420, 690, 716
Bme1390I CCNGG 4 cut(s) 62, 155, 174, 544
Bme18I GGWCC 2 cut(s) 96, 530
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 96, 530
BmiI GGNNCC 5 cut(s) 122, 178, 370, 687, 788
BmrFI CCNGG 4 cut(s) 62, 155, 174, 544
BmrI ACTGGG 2 cut(s) 747, 790
BmsI GCATC 2 cut(s) 17, 204
BmuI ACTGGG 2 cut(s) 747, 790
BpmI CTGGAG 1 cut(s) 642
BpuEI CTTGAG 2 cut(s) 242, 380
BpuMI CCSGG 2 cut(s) 62, 544
BsaAI YACGTR 2 cut(s) 424, 559
BsaJI CCNNGG 2 cut(s) 27, 124
BsaWI WCCGGW 1 cut(s) 801
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 105, 702
Bse118I RCCGGY 2 cut(s) 190, 691
Bse1I ACTGG 2 cut(s) 742, 785
BseBI CCWGG 2 cut(s) 155, 174
BseDI CCNNGG 2 cut(s) 27, 124
BseGI GGATG 2 cut(s) 747, 760
BseLI CCNNNNNNNGG 3 cut(s) 80, 105, 702
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 2 cut(s) 742, 785
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 489
BseX3I CGGCCG 1 cut(s) 693
BseXI GCAGC 3 cut(s) 287, 405, 701
BsgI GTGCAG 2 cut(s) 404, 700
Bsh1285I CGRYCG 2 cut(s) 94, 696
BshFI GGCC 4 cut(s) 26, 167, 200, 695
BshNI GGYRCC 1 cut(s) 786
BsiEI CGRYCG 2 cut(s) 94, 696
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 7 cut(s) 61, 74, 164, 191, 543, 692, 802
BsiWI CGTACG 1 cut(s) 555
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 3 cut(s) 80, 105, 702
BsmAI GTCTC 1 cut(s) 660
BsmFI GGGAC 1 cut(s) 157
BsnI GGCC 4 cut(s) 26, 167, 200, 695
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 459, 489
Bsp143I GATC 1 cut(s) 551
BspACI CCGC 2 cut(s) 188, 689
BspANI GGCC 4 cut(s) 26, 167, 200, 695
BspCNI CTCAG 1 cut(s) 634
BspLI GGNNCC 5 cut(s) 122, 178, 370, 687, 788
BspMAI CTGCAG 1 cut(s) 729
BspPI GGATC 1 cut(s) 559
BspQI GCTCTTC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 786
BspTI CTTAAG 1 cut(s) 769
BsrFI RCCGGY 2 cut(s) 190, 691
BsrI ACTGG 2 cut(s) 742, 785
BssAI RCCGGY 2 cut(s) 190, 691
BssECI CCNNGG 2 cut(s) 27, 124
BssMI GATC 1 cut(s) 551
BssNAI GTATAC 1 cut(s) 435
Bst1107I GTATAC 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 155, 174
Bst4CI ACNGT 7 cut(s) 105, 148, 373, 441, 491, 530, 721
Bst6I CTCTTC 2 cut(s) 41, 63
BstAFI CTTAAG 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 418
BstBAI YACGTR 2 cut(s) 424, 559
BstC8I GCNNGC 3 cut(s) 504, 585, 693
BstDEI CTNAG 1 cut(s) 642
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 747, 760
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 1 cut(s) 554
BstMAI GTCTC 1 cut(s) 660
BstMBI GATC 1 cut(s) 551
BstMCI CGRYCG 2 cut(s) 94, 696
BstMWI GCNNNNNNNGC 3 cut(s) 164, 418, 593
BstNI CCWGG 2 cut(s) 155, 174
BstSCI CCNGG 4 cut(s) 60, 153, 172, 542
BstSFI CTRYAG 1 cut(s) 725
BstSLI GKGCMC 1 cut(s) 489
BstV1I GCAGC 3 cut(s) 287, 405, 701
BstZ17I GTATAC 1 cut(s) 435
BstZI CGGCCG 1 cut(s) 693
BsuRI GGCC 4 cut(s) 26, 167, 200, 695
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 2 cut(s) 227, 318
BtsCI GGATG 2 cut(s) 747, 760
BtsIMutI CAGTG 3 cut(s) 487, 639, 717
Cac8I GCNNGC 3 cut(s) 504, 585, 693
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 2 cut(s) 190, 691
Cfr13I GGNCC 2 cut(s) 96, 530
CsiI ACCWGGT 1 cut(s) 172
Csp6I GTAC 3 cut(s) 556, 604, 787
CviAII CATG 3 cut(s) 196, 413, 470
CviQI GTAC 3 cut(s) 556, 604, 787
DdeI CTNAG 1 cut(s) 642
DpnI GATC 1 cut(s) 553
DpnII GATC 1 cut(s) 551
EaeI YGGCCR 3 cut(s) 165, 198, 693
EagI CGGCCG 1 cut(s) 693
Eam1104I CTCTTC 2 cut(s) 41, 63
EarI CTCTTC 2 cut(s) 41, 63
EclXI CGGCCG 1 cut(s) 693
Eco24I GRGCYC 1 cut(s) 459
Eco47I GGWCC 2 cut(s) 96, 530
Eco52I CGGCCG 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 38
EcoRII CCWGG 2 cut(s) 153, 172
EcoT38I GRGCYC 1 cut(s) 459
FaeI CATG 3 cut(s) 199, 416, 473
FaiI YATR 9 cut(s) 197, 357, 414, 435, 471, 630, 632, 651, 653
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 1 cut(s) 157
FatI CATG 3 cut(s) 195, 412, 469
FblI GTMKAC 2 cut(s) 250, 434
Fnu4HI GCNGC 5 cut(s) 188, 301, 419, 689, 715
FokI GGATG 2 cut(s) 754, 767
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 5 cut(s) 188, 301, 419, 689, 715
FspBI CTAG 2 cut(s) 159, 518
GlaI GCGC 1 cut(s) 130
GluI GCNGC 5 cut(s) 188, 301, 419, 689, 715
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 4 cut(s) 26, 167, 200, 695
HapII CCGG 7 cut(s) 61, 74, 164, 191, 543, 692, 802
HhaI GCGC 1 cut(s) 131
Hin1II CATG 3 cut(s) 199, 416, 473
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 2 cut(s) 251, 735
HindII GTYRAC 2 cut(s) 251, 735
HinfI GANTC 5 cut(s) 57, 77, 224, 385, 798
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 7 cut(s) 61, 74, 164, 191, 543, 692, 802
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 5 cut(s) 251, 435, 487, 735, 760
Hpy188I TCNGA 5 cut(s) 13, 96, 223, 390, 625
Hpy188III TCNNGA 8 cut(s) 40, 142, 259, 268, 397, 533, 659, 683
Hpy8I GTNNAC 5 cut(s) 251, 435, 487, 735, 760
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 2 cut(s) 317, 445
HpyCH4III ACNGT 7 cut(s) 105, 148, 373, 441, 491, 530, 721
HpyCH4IV ACGT 4 cut(s) 87, 342, 423, 558
HpyCH4V TGCA 6 cut(s) 8, 412, 421, 487, 717, 727
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 418, 593
HpyF3I CTNAG 1 cut(s) 642
HpySE526I ACGT 4 cut(s) 87, 342, 423, 558
Hsp92II CATG 3 cut(s) 199, 416, 473
HspAI GCGC 1 cut(s) 129
KpnI GGTACC 1 cut(s) 790
KroI GCCGGC 1 cut(s) 691
KroNI GCCGGC 1 cut(s) 693
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 1 cut(s) 551
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 3 cut(s) 287, 405, 701
LweI GCATC 2 cut(s) 17, 204
MabI ACCWGGT 1 cut(s) 172
MaeI CTAG 2 cut(s) 159, 518
MaeII ACGT 4 cut(s) 87, 342, 423, 558
MaeIII GTNAC 4 cut(s) 307, 441, 491, 524
MalI GATC 1 cut(s) 553
MboI GATC 1 cut(s) 551
MboII GAAGA 3 cut(s) 55, 58, 80
MhlI GDGCHC 2 cut(s) 459, 489
MluCI AATT 4 cut(s) 137, 263, 400, 460
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 8 cut(s) 42, 58, 134, 322, 359, 370, 423, 585
MroNI GCCGGC 1 cut(s) 691
MseI TTAA 4 cut(s) 240, 498, 734, 770
MslI CAYNNNNRTG 1 cut(s) 200
MspCI CTTAAG 1 cut(s) 769
MspI CCGG 7 cut(s) 61, 74, 164, 191, 543, 692, 802
MspR9I CCNGG 4 cut(s) 62, 155, 174, 544
MvaI CCWGG 2 cut(s) 155, 174
MwoI GCNNNNNNNGC 3 cut(s) 164, 418, 593
NaeI GCCGGC 1 cut(s) 693
NciI CCSGG 2 cut(s) 62, 544
NdeII GATC 1 cut(s) 551
NgoMIV GCCGGC 1 cut(s) 691
NlaIII CATG 3 cut(s) 199, 416, 473
NlaIV GGNNCC 5 cut(s) 122, 178, 370, 687, 788
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 4 cut(s) 307, 441, 491, 524
PaeR7I CTCGAG 1 cut(s) 38
PciSI GCTCTTC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 2 cut(s) 109, 560
PdiI GCCGGC 1 cut(s) 693
PfeI GAWTC 5 cut(s) 57, 77, 224, 385, 798
Pfl23II CGTACG 1 cut(s) 555
PflMI CCANNNNNTGG 1 cut(s) 105
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 5 cut(s) 189, 302, 420, 690, 716
Ppu21I YACGTR 2 cut(s) 424, 559
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 153, 172
PspGI CCWGG 2 cut(s) 153, 172
PspLI CGTACG 1 cut(s) 555
PspN4I GGNNCC 5 cut(s) 122, 178, 370, 687, 788
PspPI GGNCC 2 cut(s) 96, 530
PstI CTGCAG 1 cut(s) 729
RsaI GTAC 3 cut(s) 557, 605, 788
RsaNI GTAC 3 cut(s) 556, 604, 787
RseI CAYNNNNRTG 1 cut(s) 200
SalI GTCGAC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 4 cut(s) 240, 498, 734, 770
SatI GCNGC 5 cut(s) 188, 301, 419, 689, 715
Sau3AI GATC 1 cut(s) 551
Sau96I GGNCC 2 cut(s) 96, 530
ScrFI CCNGG 4 cut(s) 62, 155, 174, 544
SduI GDGCHC 2 cut(s) 459, 489
SexAI ACCWGGT 1 cut(s) 172
SfaNI GCATC 2 cut(s) 17, 204
SfcI CTRYAG 1 cut(s) 725
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 2 cut(s) 96, 530
SlaI CTCGAG 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 4 cut(s) 38, 257, 395, 769
SmoI CTYRAG 4 cut(s) 38, 257, 395, 769
Sse9I AATT 4 cut(s) 137, 263, 400, 460
SsiI CCGC 2 cut(s) 188, 689
SspMI CTAG 2 cut(s) 159, 518
StyD4I CCNGG 4 cut(s) 60, 153, 172, 542
TaaI ACNGT 7 cut(s) 105, 148, 373, 441, 491, 530, 721
TaiI ACGT 4 cut(s) 90, 345, 426, 561
TaqI TCGA 4 cut(s) 39, 112, 250, 321
TaqII GACCGA 1 cut(s) 80
TasI AATT 4 cut(s) 137, 263, 400, 460
TatI WGTACW 1 cut(s) 603
TauI GCSGC 2 cut(s) 190, 691
TfiI GAWTC 5 cut(s) 57, 77, 224, 385, 798
Tru1I TTAA 4 cut(s) 240, 498, 734, 770
Tru9I TTAA 4 cut(s) 240, 498, 734, 770
TscAI CASTG 3 cut(s) 494, 646, 724
TseFI GTSAC 4 cut(s) 307, 441, 491, 524
TseI GCWGC 3 cut(s) 300, 418, 714
Tsp45I GTSAC 4 cut(s) 307, 441, 491, 524
TspDTI ATGAA 2 cut(s) 458, 629
TspRI CASTG 3 cut(s) 494, 646, 724
Van91I CCANNNNNTGG 1 cut(s) 105
Vha464I CTTAAG 1 cut(s) 769
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 96, 530
XapI RAATTY 2 cut(s) 263, 400
XhoI CTCGAG 1 cut(s) 38
XmiI GTMKAC 2 cut(s) 250, 434
XspI CTAG 2 cut(s) 159, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.