Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001298

Physical Location & Seq

Genomic Coordinates Reverse (-)

15952647 .. 15953717

Gene Structure 1071 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1161800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGGGTTCGATGAAGAGGAAGAGACCGGACGAACCAGAGAAGAGCTTTTTCCGTTCGAAATTCGAGTGGGTTCGAGGGCCGATGCTCGGGAGTGGGGGATTCGGGTCGGTTTATCTGGCGACCGTGAAAAAGTCCAAGTTGGGTTGCGAGGGTTTCCCGACGGTGATGGCGGTGAAAACCTTCTCCAAGTCGAGTGAGCTCGCGACGGAAAGTTCGTATCTGACCCTGTTTCGGGACTGCCCTTTTATTATCGGAAGCTATGGAACAAATCTGACCATTGGGGACGATGGTTACTTGAAGTGTAATGTGTTTTTGGAGTATGCCGATGGAGGAACAATTGGGGATTTGATCGAGAAATCGGGCGGCTCAGGGTTGTGTGAATCGGATGTGAGCAAGTATACCAAAGCGATTCTGAAAGGGCTTGAGTACATTCATGAAAGGGGTTATGTGCATTGTGATTTGAAGCCGGATAACATATTGCTTGTGAGATCTGATTCTGGTTTTGTGCCGAAAATTGGGGACTTCGGGTTGGCAAAGATGGCGATGCAGAAGCTGTCACGCCAAGGCACATCAATGTATTTTTCTCCCGAAACTGTGACTCACAAAATTCAGGAGCAGCCTTCCGATATTTGGGCAGTAGGTTGCATTGTACTCGGGATGTTAACGGGGAGACAGCCATGGGAAGCGAAGGGAGGCGCTGACCTAAGGCCTCTTATTGCTTCAAAACTTCCCAATGTTCCCGGCTGGTTATCAGAAGATGCTAAAGACTTTTTGCGAAAATGCCTACTGAGGGACCCTTTCAAGAGATCCACAGTTGCTGAGCTATTGACGCACTCGTTCGTGACAAAACCGGACAGGGTTGGAGAGGTTGAACCAGTAAAGGAGGTTGCTTCAGTGTCATCTCCGGAGCAAGGCGATTATGGAAGCTTTATACCCCTCGGAAGCTGGAGTTCTGAAGAGGCAGTAGATTTTGGGCCAATTCCTCCTGTGGCAGTCTTGAACCCTAGACCGGTTATTCCAAGCACGGCTTGCCAAGGGGCATCAAATTTTGCTGTAATGGGTGCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

357

Amino Acids

38.66

Weight (kDa)

6.9

Isoelectric Point (pI)

41.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	26 - 281	7.6e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	28 - 276	6.2e-25	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001298)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35583 FvH4_6g36890 FvH4_7g03770 FvH4_7g03770 FvH4_7g04440 FvH4_7g04790
malus_domestica	MD09G1165900.v1.1 MD17G1161800.v1.1
prunus_persica	Prupe.3G023500_v2.0.a1 Prupe.3G023600_v2.0.a1 Prupe.3G058200_v2.0.a1 Prupe.3G058400_v2.0.a1 Prupe.3G109900_v2.0.a1 Prupe.3G111200_v2.0.a1 Prupe.3G111500_v2.0.a1 Prupe.3G152600_v2.0.a1 Prupe.3G152700_v2.0.a1 Prupe.3G158300_v2.0.a1 Prupe.5G038600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0147421 RchiOBHm_Chr2g0149301 RchiOBHm_Chr2g0149321
rosa_laevigata	RLG00000008572 RLG00000008574 RLG00000008575 RLG00000008577 RLG00000008579 RLG00000008580 RLG00000020260 RLG00000020391 RLG00000020392
rosa_multiflora	Rmu_sc0000873.1_g000012 Rmu_sc0000873.1_g000015 Rmu_sc0001159.1_g000028
rosa_roxburghii	Rroxscaffold_2G00098220 Rroxscaffold_2G00098230
rosa_rugosa	Rorug02G0400000.1 Rorug02G0410500 Rorug04G0092100 Rorug04G0092200 Rorug04G0114400
rosa_samantha	Rh2AG456800 Rh2AG471200 Rh2BG469600 Rh2CG444300 Rh2CG457100 Rh2DG479100 Rh2DG492400 Rh2DG492500 Rh4BG152900 Rh4BG153500 Rh7AG365800 Rh7AG366200 Rh7AG366400
rosa_wichuraiana	Rw2G038330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	398
AccII	CGCG	1 cut(s)	203
AccIII	TCCGGA	1 cut(s)	904
AciI	CCGC	2 cut(s)	170, 363
AclWI	GGATC	1 cut(s)	801
AcsI	RAATTY	3 cut(s)	59, 606, 1045
AcuI	CTGAAG	2 cut(s)	876, 975
AfaI	GTAC	2 cut(s)	428, 651
AfiI	CCNNNNNNNGG	8 cut(s)	86, 231, 232, 515, 630, 790, 911, 1009
AgeI	ACCGGT	1 cut(s)	1009
AgsI	TTSAA	6 cut(s)	298, 463, 723, 802, 872, 1000
AluBI	AGCT	8 cut(s)	45, 199, 258, 553, 823, 927, 945, 1067
AluI	AGCT	8 cut(s)	45, 199, 258, 553, 823, 927, 945, 1067
Alw21I	GWGCWC	1 cut(s)	201
Alw26I	GTCTC	2 cut(s)	16, 664
AlwI	GGATC	1 cut(s)	801
AlwNI	CAGNNNCTG	2 cut(s)	553, 818
Ama87I	CYCGRG	2 cut(s)	86, 653
Aor13HI	TCCGGA	1 cut(s)	904
AoxI	GGCC	3 cut(s)	77, 707, 974
ApeKI	GCWGC	2 cut(s)	616, 1064
ApoI	RAATTY	3 cut(s)	59, 606, 1045
ArsI	GACNNNNNNTTYG	2 cut(s)	196, 228
AsiGI	ACCGGT	1 cut(s)	1009
Asp700I	GAANNNNTTC	1 cut(s)	179
AspLEI	GCGC	1 cut(s)	698
AspS9I	GGNCC	3 cut(s)	77, 793, 974
AsuC2I	CCSGG	1 cut(s)	741
AsuHPI	GGTGA	2 cut(s)	175, 184
AsuII	TTCGAA	1 cut(s)	56
AvaI	CYCGRG	2 cut(s)	86, 653
AvaII	GGWCC	1 cut(s)	793
AxyI	CCTNAGG	1 cut(s)	704
BanII	GRGCYC	1 cut(s)	201
Bbv12I	GWGCWC	1 cut(s)	201
BbvI	GCAGC	1 cut(s)	628
BccI	CCATC	4 cut(s)	160, 281, 320, 532
BceAI	ACGGC	1 cut(s)	1041
BcgI	CGANNNNNNTGC	2 cut(s)	634, 668
BcnI	CCSGG	1 cut(s)	741
BcoDI	GTCTC	2 cut(s)	16, 664
BfaI	CTAG	1 cut(s)	1005
BfoI	RGCGCY	1 cut(s)	699
BglII	AGATCT	1 cut(s)	488
BisI	GCNGC	3 cut(s)	364, 617, 1065
BlpI	GCTNAGC	1 cut(s)	819
BlsI	GCNGC	3 cut(s)	365, 618, 1066
Bme1390I	CCNGG	1 cut(s)	741
Bme18I	GGWCC	1 cut(s)	793
BmeT110I	CYCGRG	2 cut(s)	86, 653
BmgT120I	GGNCC	3 cut(s)	77, 793, 974
BmiI	GGNNCC	2 cut(s)	794, 795
BmrFI	CCNGG	1 cut(s)	741
BmsI	GCATC	4 cut(s)	72, 534, 748, 1049
BpmI	CTGGAG	1 cut(s)	967
Bpu10I	CCTNAGC	1 cut(s)	367
Bpu1102I	GCTNAGC	1 cut(s)	819
Bpu14I	TTCGAA	1 cut(s)	56
BpuEI	CTTGAG	1 cut(s)	443
BpuMI	CCSGG	1 cut(s)	741
BsaI	GGTCTC	1 cut(s)	16
BsaJI	CCNNGG	4 cut(s)	562, 677, 937, 1033
BsaWI	WCCGGW	4 cut(s)	25, 850, 904, 1009
Bsc4I	CCNNNNNNNGG	8 cut(s)	86, 231, 232, 515, 630, 790, 911, 1009
Bse118I	RCCGGY	1 cut(s)	1009
Bse1I	ACTGG	1 cut(s)	875
Bse21I	CCTNAGG	1 cut(s)	704
BseAI	TCCGGA	1 cut(s)	904
BseDI	CCNNGG	4 cut(s)	562, 677, 937, 1033
BseGI	GGATG	2 cut(s)	391, 663
BseLI	CCNNNNNNNGG	8 cut(s)	86, 231, 232, 515, 630, 790, 911, 1009
BseMII	CTCAG	3 cut(s)	381, 779, 810
BseNI	ACTGG	1 cut(s)	875
BseXI	GCAGC	1 cut(s)	628
Bsh1236I	CGCG	1 cut(s)	203
Bsh1285I	CGRYCG	1 cut(s)	123
BshFI	GGCC	3 cut(s)	79, 709, 976
BshTI	ACCGGT	1 cut(s)	1009
BsiEI	CGRYCG	1 cut(s)	123
BsiHKAI	GWGCWC	1 cut(s)	201
BsiHKCI	CYCGRG	2 cut(s)	86, 653
BsiSI	CCGG	6 cut(s)	26, 467, 741, 851, 905, 1010
BslFI	GGGAC	4 cut(s)	248, 296, 533, 806
BslI	CCNNNNNNNGG	8 cut(s)	86, 231, 232, 515, 630, 790, 911, 1009
BsmAI	GTCTC	2 cut(s)	16, 664
BsmFI	GGGAC	4 cut(s)	248, 296, 533, 806
BsnI	GGCC	3 cut(s)	79, 709, 976
Bso31I	GGTCTC	1 cut(s)	16
BsoBI	CYCGRG	2 cut(s)	86, 653
Bsp119I	TTCGAA	1 cut(s)	56
Bsp1286I	GDGCHC	1 cut(s)	201
Bsp13I	TCCGGA	1 cut(s)	904
Bsp143I	GATC	3 cut(s)	348, 488, 806
Bsp1720I	GCTNAGC	1 cut(s)	819
Bsp19I	CCATGG	1 cut(s)	677
Bsp68I	TCGCGA	1 cut(s)	203
BspACI	CCGC	2 cut(s)	170, 363
BspANI	GGCC	3 cut(s)	79, 709, 976
BspCNI	CTCAG	3 cut(s)	380, 780, 811
BspEI	TCCGGA	1 cut(s)	904
BspFNI	CGCG	1 cut(s)	203
BspHI	TCATGA	1 cut(s)	433
BspLI	GGNNCC	2 cut(s)	794, 795
BspPI	GGATC	1 cut(s)	801
BspQI	GCTCTTC	1 cut(s)	35
BspT104I	TTCGAA	1 cut(s)	56
BspTNI	GGTCTC	1 cut(s)	16
BsrFI	RCCGGY	1 cut(s)	1009
BsrI	ACTGG	1 cut(s)	875
BssAI	RCCGGY	1 cut(s)	1009
BssECI	CCNNGG	4 cut(s)	562, 677, 937, 1033
BssMI	GATC	3 cut(s)	348, 488, 806
BssNAI	GTATAC	1 cut(s)	399
BssT1I	CCWWGG	3 cut(s)	562, 677, 1033
Bst1107I	GTATAC	1 cut(s)	399
Bst4CI	ACNGT	4 cut(s)	124, 163, 595, 814
Bst6I	CTCTTC	4 cut(s)	8, 14, 35, 951
BstAPI	GCANNNNNTGC	1 cut(s)	1029
BstBI	TTCGAA	1 cut(s)	56
BstC8I	GCNNGC	2 cut(s)	201, 1030
BstDEI	CTNAG	5 cut(s)	367, 704, 788, 819, 1068
BstDSI	CCRYGG	1 cut(s)	677
BstF5I	GGATG	2 cut(s)	391, 663
BstFNI	CGCG	1 cut(s)	203
BstH2I	RGCGCY	1 cut(s)	699
BstHHI	GCGC	1 cut(s)	698
BstKTI	GATC	3 cut(s)	351, 491, 809
BstMAI	GTCTC	2 cut(s)	16, 664
BstMBI	GATC	3 cut(s)	348, 488, 806
BstMCI	CGRYCG	1 cut(s)	123
BstMWI	GCNNNNNNNGC	3 cut(s)	539, 829, 1029
BstSCI	CCNGG	1 cut(s)	739
BstUI	CGCG	1 cut(s)	203
BstV1I	GCAGC	1 cut(s)	628
BstX2I	RGATCY	2 cut(s)	488, 806
BstYI	RGATCY	2 cut(s)	488, 806
BstZ17I	GTATAC	1 cut(s)	399
Bsu36I	CCTNAGG	1 cut(s)	704
BsuRI	GGCC	3 cut(s)	79, 709, 976
BtgI	CCRYGG	1 cut(s)	677
BtgZI	GCGATG	1 cut(s)	557
BtsCI	GGATG	2 cut(s)	391, 663
BtsIMutI	CAGTG	1 cut(s)	900
BtuMI	TCGCGA	1 cut(s)	203
Cac8I	GCNNGC	2 cut(s)	201, 1030
CaiI	CAGNNNCTG	2 cut(s)	553, 818
CciI	TCATGA	1 cut(s)	433
CfoI	GCGC	1 cut(s)	698
Cfr10I	RCCGGY	1 cut(s)	1009
Cfr13I	GGNCC	3 cut(s)	77, 793, 974
CseI	GACGC	1 cut(s)	838
Csp6I	GTAC	2 cut(s)	427, 650
CspAI	ACCGGT	1 cut(s)	1009
CviAII	CATG	2 cut(s)	434, 678
CviQI	GTAC	2 cut(s)	427, 650
DdeI	CTNAG	5 cut(s)	367, 704, 788, 819, 1068
DpnI	GATC	3 cut(s)	350, 490, 808
DpnII	GATC	3 cut(s)	348, 488, 806
Eam1104I	CTCTTC	4 cut(s)	8, 14, 35, 951
EarI	CTCTTC	4 cut(s)	8, 14, 35, 951
Ecl136II	GAGCTC	1 cut(s)	199
Eco130I	CCWWGG	3 cut(s)	562, 677, 1033
Eco147I	AGGCCT	1 cut(s)	709
Eco24I	GRGCYC	1 cut(s)	201
Eco31I	GGTCTC	1 cut(s)	16
Eco47I	GGWCC	1 cut(s)	793
Eco53kI	GAGCTC	1 cut(s)	199
Eco57I	CTGAAG	2 cut(s)	876, 975
Eco81I	CCTNAGG	1 cut(s)	704
Eco88I	CYCGRG	2 cut(s)	86, 653
EcoICRI	GAGCTC	1 cut(s)	199
EcoO109I	RGGNCCY	1 cut(s)	793
EcoT14I	CCWWGG	3 cut(s)	562, 677, 1033
EcoT38I	GRGCYC	1 cut(s)	201
ErhI	CCWWGG	3 cut(s)	562, 677, 1033
FaeI	CATG	2 cut(s)	437, 681
FaiI	YATR	9 cut(s)	261, 321, 399, 435, 447, 476, 679, 921, 932
FalI	AAGNNNNNCTT	2 cut(s)	1012, 1044
FaqI	GGGAC	4 cut(s)	248, 296, 533, 806
FatI	CATG	2 cut(s)	433, 677
FblI	GTMKAC	1 cut(s)	398
Fnu4HI	GCNGC	3 cut(s)	364, 617, 1065
FokI	GGATG	2 cut(s)	398, 670
FriOI	GRGCYC	1 cut(s)	201
Fsp4HI	GCNGC	3 cut(s)	364, 617, 1065
FspBI	CTAG	1 cut(s)	1005
GlaI	GCGC	1 cut(s)	697
GluI	GCNGC	3 cut(s)	364, 617, 1065
GsuI	CTGGAG	1 cut(s)	967
HaeII	RGCGCY	1 cut(s)	699
HaeIII	GGCC	3 cut(s)	79, 709, 976
HapII	CCGG	6 cut(s)	26, 467, 741, 851, 905, 1010
HgaI	GACGC	1 cut(s)	838
HhaI	GCGC	1 cut(s)	698
Hin1II	CATG	2 cut(s)	437, 681
Hin6I	GCGC	1 cut(s)	696
HinP1I	GCGC	1 cut(s)	696
HincII	GTYRAC	1 cut(s)	663
HindII	GTYRAC	1 cut(s)	663
HindIII	AAGCTT	1 cut(s)	925
HinfI	GANTC	5 cut(s)	99, 380, 409, 494, 598
HpaI	GTTAAC	1 cut(s)	663
HpaII	CCGG	6 cut(s)	26, 467, 741, 851, 905, 1010
HphI	GGTGA	2 cut(s)	175, 184
Hpy166II	GTNNAC	2 cut(s)	399, 663
Hpy8I	GTNNAC	2 cut(s)	399, 663
Hpy99I	CGWCG	2 cut(s)	163, 208
HpyAV	CCTTC	3 cut(s)	190, 630, 682
HpyCH4III	ACNGT	4 cut(s)	124, 163, 595, 814
HpyCH4V	TGCA	4 cut(s)	451, 547, 645, 1064
HpyF10VI	GCNNNNNNNGC	3 cut(s)	539, 829, 1029
HpyF3I	CTNAG	5 cut(s)	367, 704, 788, 819, 1068
Hsp92II	CATG	2 cut(s)	437, 681
HspAI	GCGC	1 cut(s)	696
KflI	GGGWCCC	1 cut(s)	793
Kpn2I	TCCGGA	1 cut(s)	904
KspAI	GTTAAC	1 cut(s)	663
Kzo9I	GATC	3 cut(s)	348, 488, 806
LguI	GCTCTTC	1 cut(s)	35
LmnI	GCTCC	2 cut(s)	613, 907
Lsp1109I	GCAGC	1 cut(s)	628
LweI	GCATC	4 cut(s)	72, 534, 748, 1049
MaeI	CTAG	1 cut(s)	1005
MaeIII	GTNAC	4 cut(s)	290, 555, 595, 841
MalI	GATC	3 cut(s)	350, 490, 808
MboI	GATC	3 cut(s)	348, 488, 806
MboII	GAAGA	5 cut(s)	25, 31, 52, 767, 968
MfeI	CAATTG	1 cut(s)	336
MflI	RGATCY	2 cut(s)	488, 806
MhlI	GDGCHC	1 cut(s)	201
MluCI	AATT	6 cut(s)	59, 336, 513, 606, 978, 1045
MlyI	GAGTC	1 cut(s)	592
MmeI	TCCRAC	1 cut(s)	841
MroI	TCCGGA	1 cut(s)	904
MroXI	GAANNNNTTC	1 cut(s)	179
MseI	TTAA	1 cut(s)	662
MslI	CAYNNNNRTG	1 cut(s)	572
MspI	CCGG	6 cut(s)	26, 467, 741, 851, 905, 1010
MspR9I	CCNGG	1 cut(s)	741
MunI	CAATTG	1 cut(s)	336
MvnI	CGCG	1 cut(s)	203
MwoI	GCNNNNNNNGC	3 cut(s)	539, 829, 1029
NciI	CCSGG	1 cut(s)	741
NcoI	CCATGG	1 cut(s)	677
NdeII	GATC	3 cut(s)	348, 488, 806
NlaIII	CATG	2 cut(s)	437, 681
NlaIV	GGNNCC	2 cut(s)	794, 795
NmuCI	GTSAC	3 cut(s)	555, 595, 841
NruI	TCGCGA	1 cut(s)	203
NspV	TTCGAA	1 cut(s)	56
PagI	TCATGA	1 cut(s)	433
PceI	AGGCCT	1 cut(s)	709
PciSI	GCTCTTC	1 cut(s)	35
PcsI	WCGNNNNNNNCGW	1 cut(s)	212
PdmI	GAANNNNTTC	1 cut(s)	179
PfeI	GAWTC	4 cut(s)	99, 380, 409, 494
PinAI	ACCGGT	1 cut(s)	1009
PkrI	GCNGC	3 cut(s)	365, 618, 1066
PleI	GAGTC	1 cut(s)	592
PpsI	GAGTC	1 cut(s)	592
PpuMI	RGGWCCY	1 cut(s)	793
Psp124BI	GAGCTC	1 cut(s)	201
Psp5II	RGGWCCY	1 cut(s)	793
PspN4I	GGNNCC	2 cut(s)	794, 795
PspPI	GGNCC	3 cut(s)	77, 793, 974
PspPPI	RGGWCCY	1 cut(s)	793
PstNI	CAGNNNCTG	2 cut(s)	553, 818
PsuI	RGATCY	2 cut(s)	488, 806
RruI	TCGCGA	1 cut(s)	203
RsaI	GTAC	2 cut(s)	428, 651
RsaNI	GTAC	2 cut(s)	427, 650
RseI	CAYNNNNRTG	1 cut(s)	572
SacI	GAGCTC	1 cut(s)	201
SapI	GCTCTTC	1 cut(s)	35
SaqAI	TTAA	1 cut(s)	662
SatI	GCNGC	3 cut(s)	364, 617, 1065
Sau3AI	GATC	3 cut(s)	348, 488, 806
Sau96I	GGNCC	3 cut(s)	77, 793, 974
SchI	GAGTC	1 cut(s)	592
ScrFI	CCNGG	1 cut(s)	741
SduI	GDGCHC	1 cut(s)	201
SfaNI	GCATC	4 cut(s)	72, 534, 748, 1049
SfuI	TTCGAA	1 cut(s)	56
SinI	GGWCC	1 cut(s)	793
SmiMI	CAYNNNNRTG	1 cut(s)	572
SmlI	CTYRAG	1 cut(s)	422
SmoI	CTYRAG	1 cut(s)	422
Sse9I	AATT	6 cut(s)	59, 336, 513, 606, 978, 1045
SseBI	AGGCCT	1 cut(s)	709
SsiI	CCGC	2 cut(s)	170, 363
SspMI	CTAG	1 cut(s)	1005
SstI	GAGCTC	1 cut(s)	201
StuI	AGGCCT	1 cut(s)	709
StyD4I	CCNGG	1 cut(s)	739
StyI	CCWWGG	3 cut(s)	562, 677, 1033
TaaI	ACNGT	4 cut(s)	124, 163, 595, 814
TaqI	TCGA	6 cut(s)	8, 56, 63, 73, 191, 351
TasI	AATT	6 cut(s)	59, 336, 513, 606, 978, 1045
TatI	WGTACW	2 cut(s)	426, 649
TauI	GCSGC	1 cut(s)	366
TfiI	GAWTC	4 cut(s)	99, 380, 409, 494
Tru1I	TTAA	1 cut(s)	662
Tru9I	TTAA	1 cut(s)	662
TscAI	CASTG	1 cut(s)	900
TseFI	GTSAC	3 cut(s)	555, 595, 841
TseI	GCWGC	2 cut(s)	616, 1064
Tsp45I	GTSAC	3 cut(s)	555, 595, 841
TspDTI	ATGAA	3 cut(s)	26, 422, 450
TspGWI	ACGGA	2 cut(s)	41, 221
TspRI	CASTG	1 cut(s)	900
VpaK11BI	GGWCC	1 cut(s)	793
XapI	RAATTY	3 cut(s)	59, 606, 1045
XmiI	GTMKAC	1 cut(s)	398
XmnI	GAANNNNTTC	1 cut(s)	179
XspI	CTAG	1 cut(s)	1005

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.