Rorug04G0092100

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001298
Physical Location & Seq
Reverse (-)
14194666 .. 14197156
2491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0092100.1

Sequence Viewer

Length: 849 bp
ATGTCTAATTCCGAGGCCCCGAATAACGAGCTCTCCAATGGAGCAGCAGCTATGATGATGGAAAGACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGACCCAACAGACGGCGGCATCTAACGGCGTCTTGGCCGTCAAGAAGCCGCCGTCAAAGGACCGACACAGCAAGGTCGACGGCCGGGGCCGACGCATCCGCATGCCCATCATCTGCGCGGCACGTGTCTTCCAGCTCACACGTGAGCTCGGCCACAAGTCCGACGGCCAGACCATCGAGTGGCTCCTCCGCCAGGCCGAGCCCTCCATCATCGCCGCAACCGGCACCGGCACCACTCCCGCTAGCTTCTCCACCGTCTCCGTCTCCCTCCGCGGCGGAGCCTCCTCCGGCGCCGCCTCCCTCTCCAACTCCTCCACTACTTCCTCCAACGACCACAAGCCCCTCCTCGGCGGCCCCACCCCCTTCATCCTCGGCAAGCGCATGCGCACCGACGACGACGCCAAGGACGCCGACGCCCACCACGGCGCCACCGACGGAGCAGGACCGGGAGCAGTCTCGGTCGTCGGTCACCACTCCATGAGCTCCATGCTGGGCTCCGCCGCCGCCGCCGCCCAGGGAGGGTTCTGGGCGCCGCACTACGGACAAGTCTGGAGCTTCGCCGCCACGCCGCCGCCGGAGATGATGACTCAGCAGCAGTCGGTCTCCCAACAGCATTCTTTGTTCTTGCCGCAGCAGCCGATGGGGGAAGCCTCGGCGGCCAGAGTGGGAAATTACCTTCCGGGACATCTCAATTTGTTGGCTTCTTTGTCGGGCGGGCACGGCAATTCGGGTCGGAGAGAAGACGACCCGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

282

Amino Acids

29.62

Weight (kDa)

9.2

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TCP PF03634 50 - 134 3.2e-29 TCP family transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 479
AccB1I GGYRCC 5 cut(s) 317, 323, 383, 518, 622
AccB7I CCANNNNNTGG 1 cut(s) 273
AccI GTMKAC 1 cut(s) 171
AccII CGCG 3 cut(s) 212, 366, 844
AcoI YGGCCR 5 cut(s) 129, 175, 244, 259, 750
AcvI CACGTG 2 cut(s) 218, 236
AcyI GRCGYC 7 cut(s) 123, 384, 492, 501, 507, 519, 623
AfiI CCNNNNNNNGG 6 cut(s) 106, 273, 286, 440, 612, 632
AflIII ACRYGT 2 cut(s) 217, 233
AjnI CCWGG 2 cut(s) 285, 606
AluBI AGCT 7 cut(s) 31, 50, 229, 241, 339, 576, 648
AluI AGCT 7 cut(s) 31, 50, 229, 241, 339, 576, 648
Alw21I GWGCWC 3 cut(s) 33, 243, 578
Alw26I GTCTC 4 cut(s) 355, 361, 553, 700
AoxI GGCC 9 cut(s) 15, 129, 175, 181, 244, 259, 288, 445, 750
AspLEI GCGC 6 cut(s) 212, 386, 474, 480, 521, 625
AspS9I GGNCC 5 cut(s) 16, 154, 181, 446, 536
AsuC2I CCSGG 3 cut(s) 179, 540, 774
AsuHPI GGTGA 1 cut(s) 554
AsuNHI GCTAGC 1 cut(s) 335
AvaII GGWCC 2 cut(s) 154, 536
BaeGI GKGCMC 1 cut(s) 813
BanI GGYRCC 5 cut(s) 317, 323, 383, 518, 622
BanII GRGCYC 5 cut(s) 33, 243, 297, 578, 590
BbrPI CACGTG 2 cut(s) 218, 236
BbsI GAAGAC 2 cut(s) 214, 840
Bbv12I GWGCWC 3 cut(s) 33, 243, 578
BccI CCATC 5 cut(s) 52, 209, 275, 308, 727
BceAI ACGGC 8 cut(s) 116, 123, 130, 136, 190, 274, 532, 829
BciT130I CCWGG 2 cut(s) 287, 608
BcnI CCSGG 3 cut(s) 179, 540, 774
BcoDI GTCTC 4 cut(s) 355, 361, 553, 700
BfaI CTAG 1 cut(s) 336
BfoI RGCGCY 3 cut(s) 387, 522, 626
BglI GCCNNNNNGGC 1 cut(s) 749
Bme1390I CCNGG 5 cut(s) 179, 287, 540, 608, 774
Bme18I GGWCC 2 cut(s) 154, 536
BmgT120I GGNCC 5 cut(s) 16, 154, 181, 446, 536
BmrFI CCNGG 5 cut(s) 179, 287, 540, 608, 774
BmsI GCATC 2 cut(s) 122, 198
BmtI GCTAGC 1 cut(s) 339
BpiI GAAGAC 2 cut(s) 214, 840
BpmI CTGGAG 1 cut(s) 664
BpuMI CCSGG 3 cut(s) 179, 540, 774
BsaAI YACGTR 2 cut(s) 218, 236
BsaHI GRCGYC 7 cut(s) 123, 384, 492, 501, 507, 519, 623
BsaI GGTCTC 1 cut(s) 700
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 6 cut(s) 106, 273, 286, 440, 612, 632
Bse118I RCCGGY 2 cut(s) 314, 320
BseBI CCWGG 2 cut(s) 287, 608
BseGI GGATG 2 cut(s) 189, 459
BseLI CCNNNNNNNGG 6 cut(s) 106, 273, 286, 440, 612, 632
BseMII CTCAG 1 cut(s) 695
BseRI GAGGAG 4 cut(s) 269, 367, 394, 428
BseSI GKGCMC 1 cut(s) 813
BseX3I CGGCCG 1 cut(s) 175
BseYI CCCAGC 1 cut(s) 583
Bsh1236I CGCG 3 cut(s) 212, 366, 844
Bsh1285I CGRYCG 2 cut(s) 178, 555
BshFI GGCC 9 cut(s) 17, 131, 177, 183, 246, 261, 290, 447, 752
BshNI GGYRCC 5 cut(s) 317, 323, 383, 518, 622
BsiEI CGRYCG 2 cut(s) 178, 555
BsiHKAI GWGCWC 3 cut(s) 33, 243, 578
BsiSI CCGG 7 cut(s) 178, 315, 321, 381, 539, 668, 773
BslFI GGGAC 1 cut(s) 789
BslI CCNNNNNNNGG 6 cut(s) 106, 273, 286, 440, 612, 632
BsmAI GTCTC 4 cut(s) 355, 361, 553, 700
BsmBI CGTCTC 2 cut(s) 355, 361
BsmFI GGGAC 1 cut(s) 789
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 9 cut(s) 17, 131, 177, 183, 246, 261, 290, 447, 752
Bso31I GGTCTC 1 cut(s) 700
Bsp1286I GDGCHC 6 cut(s) 33, 243, 297, 578, 590, 813
BspANI GGCC 9 cut(s) 17, 131, 177, 183, 246, 261, 290, 447, 752
BspCNI CTCAG 1 cut(s) 694
BspFNI CGCG 3 cut(s) 212, 366, 844
BspOI GCTAGC 1 cut(s) 339
BspT107I GGYRCC 5 cut(s) 317, 323, 383, 518, 622
BspTNI GGTCTC 1 cut(s) 700
BsrFI RCCGGY 2 cut(s) 314, 320
BssAI RCCGGY 2 cut(s) 314, 320
BssNI GRCGYC 7 cut(s) 123, 384, 492, 501, 507, 519, 623
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 2 cut(s) 287, 608
Bst4CI ACNGT 1 cut(s) 349
BstACI GRCGYC 7 cut(s) 123, 384, 492, 501, 507, 519, 623
BstBAI YACGTR 2 cut(s) 218, 236
BstC8I GCNNGC 5 cut(s) 197, 337, 470, 476, 809
BstDEI CTNAG 1 cut(s) 681
BstDSI CCRYGG 2 cut(s) 364, 514
BstEII GGTNACC 1 cut(s) 560
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 2 cut(s) 189, 459
BstFNI CGCG 3 cut(s) 212, 366, 844
BstH2I RGCGCY 3 cut(s) 387, 522, 626
BstHHI GCGC 6 cut(s) 212, 386, 474, 480, 521, 625
BstMAI GTCTC 4 cut(s) 355, 361, 553, 700
BstMCI CGRYCG 2 cut(s) 178, 555
BstNI CCWGG 2 cut(s) 287, 608
BstNSI RCATGY 2 cut(s) 199, 478
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 5 cut(s) 177, 285, 538, 606, 772
BstSLI GKGCMC 1 cut(s) 813
BstUI CGCG 3 cut(s) 212, 366, 844
BstV2I GAAGAC 2 cut(s) 214, 840
BstZI CGGCCG 1 cut(s) 175
BsuRI GGCC 9 cut(s) 17, 131, 177, 183, 246, 261, 290, 447, 752
BtgI CCRYGG 2 cut(s) 364, 514
BtgZI GCGATG 1 cut(s) 289
BtsCI GGATG 2 cut(s) 189, 459
Cac8I GCNNGC 5 cut(s) 197, 337, 470, 476, 809
CfoI GCGC 6 cut(s) 212, 386, 474, 480, 521, 625
Cfr10I RCCGGY 2 cut(s) 314, 320
Cfr13I GGNCC 5 cut(s) 16, 154, 181, 446, 536
Cfr42I CCGCGG 1 cut(s) 367
CseI GACGC 5 cut(s) 112, 195, 500, 509, 515
CviAII CATG 4 cut(s) 196, 475, 571, 580
DdeI CTNAG 1 cut(s) 681
DinI GGCGCC 3 cut(s) 385, 520, 624
EaeI YGGCCR 5 cut(s) 129, 175, 244, 259, 750
EagI CGGCCG 1 cut(s) 175
EciI GGCGGA 3 cut(s) 272, 384, 580
Ecl136II GAGCTC 3 cut(s) 31, 241, 576
EclXI CGGCCG 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 5 cut(s) 33, 243, 297, 578, 590
Eco31I GGTCTC 1 cut(s) 700
Eco47I GGWCC 2 cut(s) 154, 536
Eco52I CGGCCG 1 cut(s) 175
Eco53kI GAGCTC 3 cut(s) 31, 241, 576
Eco72I CACGTG 2 cut(s) 218, 236
Eco91I GGTNACC 1 cut(s) 560
EcoICRI GAGCTC 3 cut(s) 31, 241, 576
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 16
EcoO65I GGTNACC 1 cut(s) 560
EcoRII CCWGG 2 cut(s) 285, 606
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 5 cut(s) 33, 243, 297, 578, 590
EgeI GGCGCC 3 cut(s) 385, 520, 624
EheI GGCGCC 3 cut(s) 385, 520, 624
ErhI CCWWGG 1 cut(s) 495
Esp3I CGTCTC 2 cut(s) 355, 361
FaeI CATG 4 cut(s) 199, 478, 574, 583
FaiI YATR 5 cut(s) 53, 197, 476, 572, 581
FaqI GGGAC 1 cut(s) 789
FatI CATG 4 cut(s) 195, 474, 570, 579
FauI CCCGC 2 cut(s) 340, 800
FblI GTMKAC 1 cut(s) 171
FokI GGATG 2 cut(s) 176, 446
FriOI GRGCYC 5 cut(s) 33, 243, 297, 578, 590
FspAI RTGCGCAY 1 cut(s) 479
FspBI CTAG 1 cut(s) 336
FspI TGCGCA 1 cut(s) 479
GlaI GCGC 6 cut(s) 211, 385, 473, 479, 520, 624
GsaI CCCAGC 1 cut(s) 587
GsuI CTGGAG 1 cut(s) 664
HaeII RGCGCY 3 cut(s) 387, 522, 626
HaeIII GGCC 9 cut(s) 17, 131, 177, 183, 246, 261, 290, 447, 752
HapII CCGG 7 cut(s) 178, 315, 321, 381, 539, 668, 773
HgaI GACGC 5 cut(s) 112, 195, 500, 509, 515
HhaI GCGC 6 cut(s) 212, 386, 474, 480, 521, 625
Hin1I GRCGYC 7 cut(s) 123, 384, 492, 501, 507, 519, 623
Hin1II CATG 4 cut(s) 199, 478, 574, 583
Hin6I GCGC 6 cut(s) 210, 384, 472, 478, 519, 623
HinP1I GCGC 6 cut(s) 210, 384, 472, 478, 519, 623
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 1 cut(s) 679
HpaII CCGG 7 cut(s) 178, 315, 321, 381, 539, 668, 773
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 3 cut(s) 13, 256, 828
Hpy188III TCNNGA 2 cut(s) 136, 643
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 9 cut(s) 176, 189, 260, 488, 491, 494, 509, 530, 560
HpyAV CCTTC 2 cut(s) 466, 779
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 217, 235
HpyF3I CTNAG 1 cut(s) 681
HpySE526I ACGT 2 cut(s) 217, 235
Hsp92I GRCGYC 7 cut(s) 123, 384, 492, 501, 507, 519, 623
Hsp92II CATG 4 cut(s) 199, 478, 574, 583
HspAI GCGC 6 cut(s) 210, 384, 472, 478, 519, 623
KasI GGCGCC 3 cut(s) 383, 518, 622
KspI CCGCGG 1 cut(s) 367
LmnI GCTCC 8 cut(s) 41, 282, 371, 530, 542, 581, 593, 645
LweI GCATC 2 cut(s) 122, 198
MaeI CTAG 1 cut(s) 336
MaeII ACGT 2 cut(s) 217, 235
MaeIII GTNAC 1 cut(s) 560
MboII GAAGA 2 cut(s) 214, 845
MhlI GDGCHC 6 cut(s) 33, 243, 297, 578, 590, 813
MluCI AATT 4 cut(s) 7, 763, 784, 817
Mly113I GGCGCC 3 cut(s) 384, 519, 623
MlyI GAGTC 1 cut(s) 673
MmeI TCCRAC 4 cut(s) 279, 423, 444, 806
MseI TTAA 1 cut(s) 847
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 366
MspI CCGG 7 cut(s) 178, 315, 321, 381, 539, 668, 773
MspR9I CCNGG 5 cut(s) 179, 287, 540, 608, 774
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 2 cut(s) 287, 608
MvnI CGCG 3 cut(s) 212, 366, 844
NarI GGCGCC 3 cut(s) 384, 519, 623
NciI CCSGG 3 cut(s) 179, 540, 774
NheI GCTAGC 1 cut(s) 335
NlaIII CATG 4 cut(s) 199, 478, 574, 583
NmeAIII GCCGAG 5 cut(s) 222, 316, 420, 444, 725
NmuCI GTSAC 1 cut(s) 560
NsbI TGCGCA 1 cut(s) 479
NspI RCATGY 2 cut(s) 199, 478
PaeI GCATGC 2 cut(s) 199, 478
PasI CCCWGGG 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PctI GAATGC 1 cut(s) 706
PflMI CCANNNNNTGG 1 cut(s) 273
PfoI TCCNGGA 1 cut(s) 772
PleI GAGTC 1 cut(s) 673
PluTI GGCGCC 3 cut(s) 387, 522, 626
PmaCI CACGTG 2 cut(s) 218, 236
PmlI CACGTG 2 cut(s) 218, 236
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 2 cut(s) 218, 236
Psp124BI GAGCTC 3 cut(s) 33, 243, 578
Psp6I CCWGG 2 cut(s) 285, 606
PspCI CACGTG 2 cut(s) 218, 236
PspEI GGTNACC 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 2 cut(s) 285, 606
PspPI GGNCC 5 cut(s) 16, 154, 181, 446, 536
RseI CAYNNNNRTG 1 cut(s) 194
SacI GAGCTC 3 cut(s) 33, 243, 578
SacII CCGCGG 1 cut(s) 367
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 1 cut(s) 847
Sau96I GGNCC 5 cut(s) 16, 154, 181, 446, 536
SchI GAGTC 1 cut(s) 673
ScrFI CCNGG 5 cut(s) 179, 287, 540, 608, 774
SduI GDGCHC 6 cut(s) 33, 243, 297, 578, 590, 813
SfaNI GCATC 2 cut(s) 122, 198
SfoI GGCGCC 3 cut(s) 385, 520, 624
Sfr303I CCGCGG 1 cut(s) 367
SgrBI CCGCGG 1 cut(s) 367
SinI GGWCC 2 cut(s) 154, 536
SmiMI CAYNNNNRTG 1 cut(s) 194
SphI GCATGC 2 cut(s) 199, 478
Sse9I AATT 4 cut(s) 7, 763, 784, 817
SspDI GGCGCC 3 cut(s) 383, 518, 622
SspMI CTAG 1 cut(s) 336
SstI GAGCTC 3 cut(s) 33, 243, 578
StyD4I CCNGG 5 cut(s) 177, 285, 538, 606, 772
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 2 cut(s) 220, 238
TaqI TCGA 2 cut(s) 171, 270
TaqII GACCGA 4 cut(s) 171, 541, 548, 682
TasI AATT 4 cut(s) 7, 763, 784, 817
Tru1I TTAA 1 cut(s) 847
Tru9I TTAA 1 cut(s) 847
TseFI GTSAC 1 cut(s) 560
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 1 cut(s) 448
TspGWI ACGGA 3 cut(s) 343, 543, 648
Van91I CCANNNNNTGG 1 cut(s) 273
VpaK11BI GGWCC 2 cut(s) 154, 536
XagI CCTNNNNNAGG 1 cut(s) 284
XceI RCATGY 2 cut(s) 199, 478
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.