Rh4BG152900

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001298
Physical Location & Seq
Forward (+)
27543594 .. 27544673
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG152900.1

Sequence Viewer

Length: 1080 bp
ATGATGGGTTTGAAAAGGAAAAGAAGCAGTGAGTTGGGTGAGTGGGTTTTGGGAGATTTGATTGGCAAGGGGGGTTTTGGGAGTGTGTATAAGGCCTATCCACTTAAGCTCGAGAAAGGGTCTCCCGCTATCATGGCCGTTAAGGTCGCTGAGGTTTCTGATCCACAAAAGGTCGATGCTTTATTGACCGAGGCCAAACTTCTCTCTCAGTTTCAGGACTGCCCTTTTGTTATCAACTCTTTTGGAGAACAGTGGACATATAAGTCTCATCATAATTCTTTCTCTTTCAATCTGCTATTAGAATTTGCGGCCGGAGGCACTATAAGAGATAGGATTCAGAAATCCGCATCCGGCTTGTCCGAATGTGAGATTAAAGAGTTCACCAGGTCAGTTCTTAGTGGTCTCCAGTGCATTCATGACAAAGGTTATGTGCATTGCGACATAAAGCCCGATAACATTCTTCTTGTGCACGACATGACTTCTCATGTCGATTTTGTGGCTAAGATAGGAGACTTTGGATTGACAAAGAGCGCTCAAAATGAGTATTCTCGCCAAGGCACCGCATGGTATTTGCCTCCTGAAACTGTTATCAGTAACATTCAAGGGAAGCCATCAGACATTTGGGCACTAGGATGTTTAGTTCTAGAGATGTTGACTGGAACCTTGTGGCCTTGCAAGACAAGTGAGGACTTAGGCGGTTGGGTTTCAAATCCAATTATTCCTTCACACTTGTCCGAGGAGGCCAAGGATTTTTTGGCAAAATGTCTGCATATAGATGCTTCCAAGAGATTTACGGCTGAGAGGCTCTTGACTCACTCATTTCTTATGAGTAAACAGAGCGTTGAACAAGTAGCTAAAGTGCCCGCATTGTCACAATCTCAGCATTGTGATAATCGGTTGCCTAGTTTTATACCCTTAGGGGACTCTAGCTCCCAAGAGGCACAAAAGAACAAAGAGGGTTCTGGTTTATATGATAATACCATTTTTCCTTTGGCACTCATGTCAAGACCTGCTAGACCTGTGGTTGCAGACACATGGTGCATAAAGCCAACAACTTTTGTTGTAATGGGCGCCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

359

Amino Acids

39.54

Weight (kDa)

6.56

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 18 - 275 1.8e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 18 - 270 9.3e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 1018
AccB1I GGYRCC 2 cut(s) 557, 1070
AciI CCGC 7 cut(s) 126, 308, 345, 561, 696, 864, 1074
AclWI GGATC 1 cut(s) 155
AcoI YGGCCR 2 cut(s) 135, 309
AcsI RAATTY 1 cut(s) 302
AcyI GRCGYC 1 cut(s) 1071
AdeI CACNNNGTG 1 cut(s) 1038
AfeI AGCGCT 1 cut(s) 532
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 5 cut(s) 13, 289, 602, 708, 845
AjnI CCWGG 1 cut(s) 383
AjuI GAANNNNNNNTTGG 2 cut(s) 706, 738
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
AluBI AGCT 3 cut(s) 109, 854, 930
AluI AGCT 3 cut(s) 109, 854, 930
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 4 cut(s) 126, 270, 407, 504
Alw44I GTGCAC 1 cut(s) 467
AlwI GGATC 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 110
Aor51HI AGCGCT 1 cut(s) 532
AoxI GGCC 6 cut(s) 93, 135, 192, 309, 668, 741
ApaLI GTGCAC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 302
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AspLEI GCGC 2 cut(s) 533, 1073
AsuHPI GGTGA 2 cut(s) 50, 373
AvaI CYCGRG 1 cut(s) 110
AxyI CCTNAGG 1 cut(s) 916
BaeGI GKGCMC 3 cut(s) 471, 628, 864
BanI GGYRCC 2 cut(s) 557, 1070
Bbv12I GWGCWC 1 cut(s) 471
BbvCI CCTCAGC 1 cut(s) 150
BccI CCATC 1 cut(s) 619
BceAI ACGGC 2 cut(s) 122, 810
BciT130I CCWGG 1 cut(s) 385
BcoDI GTCTC 4 cut(s) 126, 270, 407, 504
BfaI CTAG 5 cut(s) 629, 644, 903, 927, 1014
BfoI RGCGCY 2 cut(s) 534, 1074
BfrI CTTAAG 1 cut(s) 104
BfuAI ACCTGC 1 cut(s) 1018
BisI GCNGC 2 cut(s) 309, 1074
BlsI GCNGC 2 cut(s) 310, 1075
Bme1390I CCNGG 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 110
BmiI GGNNCC 3 cut(s) 559, 661, 1072
BmrFI CCNGG 1 cut(s) 385
BmsI GCATC 3 cut(s) 166, 356, 766
BpmI CTGGAG 1 cut(s) 389
Bpu10I CCTNAGC 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 1071
BsaI GGTCTC 2 cut(s) 126, 407
BsaJI CCNNGG 4 cut(s) 189, 553, 735, 744
BsaXI ACNNNNNCTCC 2 cut(s) 914, 944
Bse1I ACTGG 2 cut(s) 406, 661
Bse21I CCTNAGG 1 cut(s) 916
Bse3DI GCAATG 1 cut(s) 433
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 4 cut(s) 189, 553, 735, 744
BseGI GGATG 2 cut(s) 347, 638
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 4 cut(s) 141, 221, 789, 893
BseNI ACTGG 2 cut(s) 406, 661
BseRI GAGGAG 1 cut(s) 752
BseSI GKGCMC 3 cut(s) 471, 628, 864
BseX3I CGGCCG 1 cut(s) 309
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 6 cut(s) 95, 137, 194, 311, 670, 743
BshNI GGYRCC 2 cut(s) 557, 1070
BsiEI CGRYCG 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 312, 351
BslFI GGGAC 1 cut(s) 935
BsmAI GTCTC 4 cut(s) 126, 270, 407, 504
BsmFI GGGAC 1 cut(s) 935
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 6 cut(s) 95, 137, 194, 311, 670, 743
Bso31I GGTCTC 2 cut(s) 126, 407
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 3 cut(s) 471, 628, 864
Bsp143I GATC 1 cut(s) 160
BspACI CCGC 7 cut(s) 126, 308, 345, 561, 696, 864, 1074
BspANI GGCC 6 cut(s) 95, 137, 194, 311, 670, 743
BspCNI CTCAG 4 cut(s) 142, 220, 790, 892
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 559, 661, 1072
BspMI ACCTGC 1 cut(s) 1018
BspPI GGATC 1 cut(s) 155
BspT107I GGYRCC 2 cut(s) 557, 1070
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 2 cut(s) 126, 407
BsrDI GCAATG 1 cut(s) 433
BsrI ACTGG 2 cut(s) 406, 661
BssECI CCNNGG 4 cut(s) 189, 553, 735, 744
BssMI GATC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 1071
BssT1I CCWWGG 2 cut(s) 553, 744
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 252, 586
BstACI GRCGYC 1 cut(s) 1071
BstAFI CTTAAG 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 864
BstDEI CTNAG 8 cut(s) 150, 207, 395, 501, 691, 798, 879, 916
BstF5I GGATG 2 cut(s) 347, 638
BstH2I RGCGCY 2 cut(s) 534, 1074
BstHHI GCGC 2 cut(s) 533, 1073
BstKTI GATC 1 cut(s) 163
BstMAI GTCTC 4 cut(s) 126, 270, 407, 504
BstMBI GATC 1 cut(s) 160
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 383
BstSLI GKGCMC 3 cut(s) 471, 628, 864
BstZI CGGCCG 1 cut(s) 309
Bsu36I CCTNAGG 1 cut(s) 916
BsuRI GGCC 6 cut(s) 95, 137, 194, 311, 670, 743
BtsCI GGATG 2 cut(s) 347, 638
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 3 cut(s) 34, 257, 413
BveI ACCTGC 1 cut(s) 1018
Cac8I GCNNGC 1 cut(s) 864
CciI TCATGA 1 cut(s) 415
CfoI GCGC 2 cut(s) 533, 1073
CsiI ACCWGGT 1 cut(s) 383
CviAII CATG 7 cut(s) 133, 416, 475, 485, 564, 1000, 1035
DdeI CTNAG 8 cut(s) 150, 207, 395, 501, 691, 798, 879, 916
DinI GGCGCC 1 cut(s) 1072
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
DraIII CACNNNGTG 1 cut(s) 1038
DrdI GACNNNNNNGTC 1 cut(s) 262
DseDI GACNNNNNNGTC 1 cut(s) 262
EaeI YGGCCR 2 cut(s) 135, 309
EagI CGGCCG 1 cut(s) 309
EclXI CGGCCG 1 cut(s) 309
Eco130I CCWWGG 2 cut(s) 553, 744
Eco147I AGGCCT 1 cut(s) 95
Eco31I GGTCTC 2 cut(s) 126, 407
Eco47III AGCGCT 1 cut(s) 532
Eco52I CGGCCG 1 cut(s) 309
Eco81I CCTNAGG 1 cut(s) 916
Eco88I CYCGRG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 383
EcoT14I CCWWGG 2 cut(s) 553, 744
EgeI GGCGCC 1 cut(s) 1072
EheI GGCGCC 1 cut(s) 1072
ErhI CCWWGG 2 cut(s) 553, 744
FaeI CATG 7 cut(s) 136, 419, 478, 488, 567, 1003, 1038
FaqI GGGAC 1 cut(s) 935
FatI CATG 7 cut(s) 132, 415, 474, 484, 563, 999, 1034
FauI CCCGC 2 cut(s) 133, 871
Fnu4HI GCNGC 2 cut(s) 309, 1074
FokI GGATG 2 cut(s) 334, 645
Fsp4HI GCNGC 2 cut(s) 309, 1074
FspBI CTAG 5 cut(s) 629, 644, 903, 927, 1014
GlaI GCGC 2 cut(s) 532, 1072
GluI GCNGC 2 cut(s) 309, 1074
GsuI CTGGAG 1 cut(s) 389
HaeII RGCGCY 2 cut(s) 534, 1074
HaeIII GGCC 6 cut(s) 95, 137, 194, 311, 670, 743
HapII CCGG 2 cut(s) 312, 351
HhaI GCGC 2 cut(s) 533, 1073
Hin1I GRCGYC 1 cut(s) 1071
Hin1II CATG 7 cut(s) 136, 419, 478, 488, 567, 1003, 1038
Hin6I GCGC 2 cut(s) 531, 1071
HinP1I GCGC 2 cut(s) 531, 1071
HincII GTYRAC 1 cut(s) 654
HindII GTYRAC 1 cut(s) 654
HinfI GANTC 3 cut(s) 334, 811, 923
HpaII CCGG 2 cut(s) 312, 351
HphI GGTGA 2 cut(s) 50, 373
Hpy166II GTNNAC 5 cut(s) 255, 381, 469, 654, 833
Hpy188I TCNGA 5 cut(s) 160, 339, 361, 616, 736
Hpy188III TCNNGA 7 cut(s) 112, 215, 416, 578, 644, 808, 1005
Hpy8I GTNNAC 5 cut(s) 255, 381, 469, 654, 833
HpyAV CCTTC 1 cut(s) 732
HpyCH4III ACNGT 2 cut(s) 252, 586
HpyCH4V TGCA 7 cut(s) 411, 433, 469, 675, 769, 1028, 1041
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 8 cut(s) 150, 207, 395, 501, 691, 798, 879, 916
Hsp92I GRCGYC 1 cut(s) 1071
Hsp92II CATG 7 cut(s) 136, 419, 478, 488, 567, 1003, 1038
HspAI GCGC 2 cut(s) 531, 1071
KasI GGCGCC 1 cut(s) 1070
Kzo9I GATC 1 cut(s) 160
LmnI GCTCC 1 cut(s) 935
LweI GCATC 3 cut(s) 166, 356, 766
MabI ACCWGGT 1 cut(s) 383
MaeI CTAG 5 cut(s) 629, 644, 903, 927, 1014
MaeIII GTNAC 2 cut(s) 593, 870
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 1 cut(s) 452
MhlI GDGCHC 3 cut(s) 471, 628, 864
MluCI AATT 3 cut(s) 274, 302, 714
Mly113I GGCGCC 1 cut(s) 1071
MlyI GAGTC 2 cut(s) 805, 917
MseI TTAA 3 cut(s) 105, 141, 372
MslI CAYNNNNRTG 2 cut(s) 631, 774
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 2 cut(s) 312, 351
MspR9I CCNGG 1 cut(s) 385
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 1 cut(s) 134
NarI GGCGCC 1 cut(s) 1071
NdeII GATC 1 cut(s) 160
NlaIII CATG 7 cut(s) 136, 419, 478, 488, 567, 1003, 1038
NlaIV GGNNCC 3 cut(s) 559, 661, 1072
NmuCI GTSAC 1 cut(s) 870
PaeR7I CTCGAG 1 cut(s) 110
PagI TCATGA 1 cut(s) 415
PceI AGGCCT 1 cut(s) 95
PctI GAATGC 1 cut(s) 411
PfeI GAWTC 1 cut(s) 334
PkrI GCNGC 2 cut(s) 310, 1075
PleI GAGTC 2 cut(s) 805, 917
PluTI GGCGCC 1 cut(s) 1074
PpsI GAGTC 2 cut(s) 805, 917
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 3 cut(s) 559, 661, 1072
RseI CAYNNNNRTG 2 cut(s) 631, 774
SaqAI TTAA 3 cut(s) 105, 141, 372
SatI GCNGC 2 cut(s) 309, 1074
Sau3AI GATC 1 cut(s) 160
SchI GAGTC 2 cut(s) 805, 917
ScrFI CCNGG 1 cut(s) 385
SduI GDGCHC 3 cut(s) 471, 628, 864
SexAI ACCWGGT 1 cut(s) 383
SfaNI GCATC 3 cut(s) 166, 356, 766
SfoI GGCGCC 1 cut(s) 1072
Sfr274I CTCGAG 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 631, 774
SmlI CTYRAG 2 cut(s) 104, 110
SmoI CTYRAG 2 cut(s) 104, 110
Sse9I AATT 3 cut(s) 274, 302, 714
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 7 cut(s) 126, 308, 345, 561, 696, 864, 1074
SspDI GGCGCC 1 cut(s) 1070
SspMI CTAG 5 cut(s) 629, 644, 903, 927, 1014
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 383
StyI CCWWGG 2 cut(s) 553, 744
TaaI ACNGT 2 cut(s) 252, 586
TaqI TCGA 3 cut(s) 111, 174, 489
TaqII GACCGA 1 cut(s) 203
TasI AATT 3 cut(s) 274, 302, 714
TauI GCSGC 2 cut(s) 311, 1076
TfiI GAWTC 1 cut(s) 334
Tru1I TTAA 3 cut(s) 105, 141, 372
Tru9I TTAA 3 cut(s) 105, 141, 372
TscAI CASTG 3 cut(s) 34, 257, 413
TseFI GTSAC 1 cut(s) 870
Tsp45I GTSAC 1 cut(s) 870
TspDTI ATGAA 1 cut(s) 404
TspRI CASTG 3 cut(s) 34, 257, 413
Vha464I CTTAAG 1 cut(s) 104
VneI GTGCAC 1 cut(s) 467
XapI RAATTY 1 cut(s) 302
XbaI TCTAGA 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 3 cut(s) 618, 751, 988
XhoI CTCGAG 1 cut(s) 110
XspI CTAG 5 cut(s) 629, 644, 903, 927, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.