RchiOBHm_Chr2g0149321

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001298
Physical Location & Seq
Forward (+)
67198922 .. 67199935
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51873

Sequence Viewer

Length: 1014 bp
ATGACTGCATCAGACTATTTTAAGGCCATGGAAAAGCTCGAGAAGAAGAGGAAAAGAATCCAGGAGGAAGAACCGGATTCTGGCAACGTCGGTCGGACCAAACGGTGGGTTCGACAAAGGTTCCTCGGCGCAGGACAATTCGGGACGGTTTTCTTGGCTAGTCCGGCCAAACCAGGTTCCAAACTGCCGCCGGTTATGGCCGTGAAGTCGGCGATGCTCTCGGAATCTGCCGACTTGGTTAAAGAAAGTCGACTTCTCAAGAAATTCAAGAACTGCCCTTTTATCCTTGACACTTACGGCAGCGATGTGACTAAAGATGTTGAAGGTGAGGAGTTGTTCAACGTTTTCTTGGAGTATGCTCAAAGAGGAACCGTAGAGGATTGGATTCAGAAACTCAAGAATTTCGGGTTGCATGAACTGCACGTAAGGAGGTATACGCAGTCACTATTGAAGGGGCTCAATTACATTCATGGAAAGGGTTTTGTGCACTGTGACATTAAGCCCGCCAATGTATTGCTAGTGCGCGACGGTCCTGATGATACCGGGTTTGGATCTTACGTGGCGAAAATCGGGGATTTAGGGCTGGCAAAGAGGGCTGACAAGTACAATGTGATGAAGCGTTCTGATTATATAAATGGCACTGAGTTGTATATGTCTCCAGAAGTATCGCTTCACAACATTCAGGAGCCGCCGGCCGACATTTGGGCATTAGGCTGCACTGTTCTGCAGATGTTAACTGGGATGGAGTTTTGGGATGTAAACACTGACTTAAGAGATGACTGGGGCCTTCTGATTGAGATTCCGGTTATCCCTGAAAGGCTAAGCAATGAGGCTAAGGATTTTTTGGGGAAGTGCTTCATATTGGACCCTTTGGAGAGGTACACGGCTGAGATGCTTTTGAAGCCTTTCGTGTGCAACAGCTTGCGAATATGGACAGTCTCGTCTGGTGAATTGGTTCCAAGAAGCCGGGTTTGCCAAGGACCAGCTGCAATTCCTCTACTGGGTGCGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

337

Amino Acids

37.95

Weight (kDa)

7.58

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 38 - 301 2.8e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 40 - 299 5e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 940
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 2 cut(s) 250, 434
AccII CGCG 1 cut(s) 525
AciI CCGC 4 cut(s) 188, 504, 689, 1007
AclI AACGTT 1 cut(s) 342
AclWI GGATC 1 cut(s) 559
AcoI YGGCCR 3 cut(s) 165, 198, 693
AcsI RAATTY 2 cut(s) 263, 400
AfaI GTAC 2 cut(s) 605, 881
AfiI CCNNNNNNNGG 4 cut(s) 80, 105, 702, 1001
AflII CTTAAG 1 cut(s) 769
AgsI TTSAA 5 cut(s) 268, 323, 340, 451, 901
AjnI CCWGG 2 cut(s) 60, 172
AluBI AGCT 3 cut(s) 37, 921, 986
AluI AGCT 3 cut(s) 37, 921, 986
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 2 cut(s) 660, 943
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 1 cut(s) 559
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 5 cut(s) 24, 165, 198, 693, 784
ApaLI GTGCAC 1 cut(s) 485
ApeKI GCWGC 3 cut(s) 300, 714, 986
ApoI RAATTY 2 cut(s) 263, 400
Asp700I GAANNNNTTC 3 cut(s) 854, 905, 954
AspLEI GCGC 2 cut(s) 131, 525
AspS9I GGNCC 5 cut(s) 96, 530, 784, 865, 980
AsuC2I CCSGG 2 cut(s) 544, 968
AsuHPI GGTGA 2 cut(s) 338, 959
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 4 cut(s) 96, 530, 865, 980
BaeGI GKGCMC 1 cut(s) 489
BanII GRGCYC 1 cut(s) 459
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 3 cut(s) 312, 701, 973
BccI CCATC 1 cut(s) 736
BceAI ACGGC 3 cut(s) 185, 313, 900
BciT130I CCWGG 2 cut(s) 62, 174
BcnI CCSGG 2 cut(s) 544, 968
BcoDI GTCTC 2 cut(s) 660, 943
BfaI CTAG 2 cut(s) 159, 518
BfmI CTRYAG 1 cut(s) 725
BfrI CTTAAG 1 cut(s) 769
BisI GCNGC 6 cut(s) 188, 301, 689, 715, 987, 1008
BlpI GCTNAGC 1 cut(s) 821
BlsI GCNGC 6 cut(s) 189, 302, 690, 716, 988, 1009
Bme1390I CCNGG 4 cut(s) 62, 174, 544, 968
Bme18I GGWCC 4 cut(s) 96, 530, 865, 980
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 5 cut(s) 96, 530, 784, 865, 980
BmiI GGNNCC 7 cut(s) 122, 178, 370, 687, 785, 867, 957
BmrFI CCNGG 4 cut(s) 62, 174, 544, 968
BmrI ACTGGG 3 cut(s) 747, 790, 1010
BmsI GCATC 3 cut(s) 17, 204, 882
BmuI ACTGGG 3 cut(s) 747, 790, 1010
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 834
Bpu1102I GCTNAGC 1 cut(s) 821
BpuEI CTTGAG 2 cut(s) 242, 380
BpuMI CCSGG 2 cut(s) 544, 968
BsaAI YACGTR 2 cut(s) 424, 559
BsaJI CCNNGG 3 cut(s) 27, 124, 976
BsaWI WCCGGW 2 cut(s) 73, 802
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 105, 702, 1001
Bse118I RCCGGY 2 cut(s) 190, 691
Bse1I ACTGG 3 cut(s) 742, 785, 1005
Bse3DI GCAATG 1 cut(s) 832
BseBI CCWGG 2 cut(s) 62, 174
BseDI CCNNGG 3 cut(s) 27, 124, 976
BseGI GGATG 2 cut(s) 747, 760
BseLI CCNNNNNNNGG 4 cut(s) 80, 105, 702, 1001
BseMI GCAATG 1 cut(s) 832
BseMII CTCAG 2 cut(s) 633, 879
BseNI ACTGG 3 cut(s) 742, 785, 1005
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 489
BseX3I CGGCCG 1 cut(s) 693
BseXI GCAGC 3 cut(s) 312, 701, 973
BsgI GTGCAG 2 cut(s) 404, 700
Bsh1236I CGCG 1 cut(s) 525
Bsh1285I CGRYCG 2 cut(s) 94, 696
BshFI GGCC 5 cut(s) 26, 167, 200, 695, 786
BsiEI CGRYCG 2 cut(s) 94, 696
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 7 cut(s) 74, 164, 191, 543, 692, 803, 967
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 4 cut(s) 80, 105, 702, 1001
BsmAI GTCTC 2 cut(s) 660, 943
BsmFI GGGAC 1 cut(s) 157
BsnI GGCC 5 cut(s) 26, 167, 200, 695, 786
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 459, 489
Bsp143I GATC 1 cut(s) 551
Bsp1720I GCTNAGC 1 cut(s) 821
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 4 cut(s) 188, 504, 689, 1007
BspANI GGCC 5 cut(s) 26, 167, 200, 695, 786
BspCNI CTCAG 2 cut(s) 634, 880
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 7 cut(s) 122, 178, 370, 687, 785, 867, 957
BspMAI CTGCAG 1 cut(s) 729
BspPI GGATC 1 cut(s) 559
BspTI CTTAAG 1 cut(s) 769
BsrDI GCAATG 1 cut(s) 832
BsrFI RCCGGY 2 cut(s) 190, 691
BsrI ACTGG 3 cut(s) 742, 785, 1005
BssAI RCCGGY 2 cut(s) 190, 691
BssECI CCNNGG 3 cut(s) 27, 124, 976
BssMI GATC 1 cut(s) 551
BssNAI GTATAC 1 cut(s) 435
BssT1I CCWWGG 2 cut(s) 27, 976
Bst1107I GTATAC 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 62, 174
Bst4CI ACNGT 7 cut(s) 105, 148, 373, 491, 530, 721, 937
Bst6I CTCTTC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 418
BstBAI YACGTR 2 cut(s) 424, 559
BstC8I GCNNGC 4 cut(s) 504, 585, 693, 923
BstDEI CTNAG 4 cut(s) 642, 821, 834, 888
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 747, 760
BstFNI CGCG 1 cut(s) 525
BstHHI GCGC 2 cut(s) 131, 525
BstKTI GATC 1 cut(s) 554
BstMAI GTCTC 2 cut(s) 660, 943
BstMBI GATC 1 cut(s) 551
BstMCI CGRYCG 2 cut(s) 94, 696
BstMWI GCNNNNNNNGC 5 cut(s) 164, 418, 593, 901, 972
BstNI CCWGG 2 cut(s) 62, 174
BstSCI CCNGG 4 cut(s) 60, 172, 542, 966
BstSFI CTRYAG 1 cut(s) 725
BstSLI GKGCMC 1 cut(s) 489
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 3 cut(s) 312, 701, 973
BstX2I RGATCY 1 cut(s) 551
BstYI RGATCY 1 cut(s) 551
BstZ17I GTATAC 1 cut(s) 435
BstZI CGGCCG 1 cut(s) 693
BsuRI GGCC 5 cut(s) 26, 167, 200, 695, 786
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 2 cut(s) 227, 318
BtsCI GGATG 2 cut(s) 747, 760
BtsIMutI CAGTG 4 cut(s) 487, 639, 717, 762
Cac8I GCNNGC 4 cut(s) 504, 585, 693, 923
CfoI GCGC 2 cut(s) 131, 525
Cfr10I RCCGGY 2 cut(s) 190, 691
Cfr13I GGNCC 5 cut(s) 96, 530, 784, 865, 980
CsiI ACCWGGT 1 cut(s) 172
Csp6I GTAC 2 cut(s) 604, 880
CviAII CATG 3 cut(s) 28, 413, 470
CviQI GTAC 2 cut(s) 604, 880
DdeI CTNAG 4 cut(s) 642, 821, 834, 888
DpnI GATC 1 cut(s) 553
DpnII GATC 1 cut(s) 551
DrdI GACNNNNNNGTC 1 cut(s) 940
DseDI GACNNNNNNGTC 1 cut(s) 940
EaeI YGGCCR 3 cut(s) 165, 198, 693
EagI CGGCCG 1 cut(s) 693
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 693
Eco130I CCWWGG 2 cut(s) 27, 976
Eco24I GRGCYC 1 cut(s) 459
Eco47I GGWCC 4 cut(s) 96, 530, 865, 980
Eco52I CGGCCG 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 784
EcoRII CCWGG 2 cut(s) 60, 172
EcoT14I CCWWGG 2 cut(s) 27, 976
EcoT38I GRGCYC 1 cut(s) 459
ErhI CCWWGG 2 cut(s) 27, 976
FaeI CATG 3 cut(s) 31, 416, 473
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 1 cut(s) 157
FatI CATG 3 cut(s) 27, 412, 469
FauI CCCGC 1 cut(s) 511
FblI GTMKAC 2 cut(s) 250, 434
Fnu4HI GCNGC 6 cut(s) 188, 301, 689, 715, 987, 1008
FokI GGATG 2 cut(s) 754, 767
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 6 cut(s) 188, 301, 689, 715, 987, 1008
FspBI CTAG 2 cut(s) 159, 518
GlaI GCGC 2 cut(s) 130, 524
GluI GCNGC 6 cut(s) 188, 301, 689, 715, 987, 1008
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 5 cut(s) 26, 167, 200, 695, 786
HapII CCGG 7 cut(s) 74, 164, 191, 543, 692, 803, 967
HhaI GCGC 2 cut(s) 131, 525
Hin1II CATG 3 cut(s) 31, 416, 473
Hin6I GCGC 2 cut(s) 129, 523
HinP1I GCGC 2 cut(s) 129, 523
HincII GTYRAC 2 cut(s) 251, 735
HindII GTYRAC 2 cut(s) 251, 735
HinfI GANTC 5 cut(s) 57, 77, 224, 385, 799
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 7 cut(s) 74, 164, 191, 543, 692, 803, 967
HphI GGTGA 2 cut(s) 338, 959
Hpy166II GTNNAC 6 cut(s) 251, 435, 487, 735, 760, 882
Hpy188I TCNGA 6 cut(s) 13, 96, 223, 390, 625, 792
Hpy188III TCNNGA 8 cut(s) 40, 142, 259, 268, 397, 533, 659, 683
Hpy8I GTNNAC 6 cut(s) 251, 435, 487, 735, 760, 882
Hpy99I CGWCG 2 cut(s) 92, 530
HpyAV CCTTC 3 cut(s) 317, 445, 797
HpyCH4III ACNGT 7 cut(s) 105, 148, 373, 491, 530, 721, 937
HpyCH4IV ACGT 4 cut(s) 87, 342, 423, 558
HpyCH4V TGCA 8 cut(s) 8, 412, 421, 487, 717, 727, 915, 989
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 418, 593, 901, 972
HpyF3I CTNAG 4 cut(s) 642, 821, 834, 888
HpySE526I ACGT 4 cut(s) 87, 342, 423, 558
Hsp92II CATG 3 cut(s) 31, 416, 473
HspAI GCGC 2 cut(s) 129, 523
KroI GCCGGC 1 cut(s) 691
KroNI GCCGGC 1 cut(s) 693
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 1 cut(s) 551
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 3 cut(s) 312, 701, 973
LweI GCATC 3 cut(s) 17, 204, 882
MabI ACCWGGT 1 cut(s) 172
MaeI CTAG 2 cut(s) 159, 518
MaeII ACGT 4 cut(s) 87, 342, 423, 558
MaeIII GTNAC 3 cut(s) 307, 441, 491
MalI GATC 1 cut(s) 553
MboI GATC 1 cut(s) 551
MboII GAAGA 3 cut(s) 55, 58, 80
MflI RGATCY 1 cut(s) 551
MhlI GDGCHC 2 cut(s) 459, 489
MluCI AATT 6 cut(s) 137, 263, 400, 460, 950, 990
MmeI TCCRAC 1 cut(s) 74
MroNI GCCGGC 1 cut(s) 691
MroXI GAANNNNTTC 3 cut(s) 854, 905, 954
MseI TTAA 5 cut(s) 21, 240, 498, 734, 770
MspA1I CMGCKG 1 cut(s) 986
MspCI CTTAAG 1 cut(s) 769
MspI CCGG 7 cut(s) 74, 164, 191, 543, 692, 803, 967
MspR9I CCNGG 4 cut(s) 62, 174, 544, 968
MvaI CCWGG 2 cut(s) 62, 174
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 5 cut(s) 164, 418, 593, 901, 972
NaeI GCCGGC 1 cut(s) 693
NciI CCSGG 2 cut(s) 544, 968
NcoI CCATGG 1 cut(s) 27
NdeII GATC 1 cut(s) 551
NgoMIV GCCGGC 1 cut(s) 691
NlaIII CATG 3 cut(s) 31, 416, 473
NlaIV GGNNCC 7 cut(s) 122, 178, 370, 687, 785, 867, 957
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 3 cut(s) 307, 441, 491
PaeR7I CTCGAG 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 109
PdiI GCCGGC 1 cut(s) 693
PdmI GAANNNNTTC 3 cut(s) 854, 905, 954
PfeI GAWTC 5 cut(s) 57, 77, 224, 385, 799
PflMI CCANNNNNTGG 1 cut(s) 105
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 6 cut(s) 189, 302, 690, 716, 988, 1009
Ppu21I YACGTR 2 cut(s) 424, 559
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 60, 172
PspGI CCWGG 2 cut(s) 60, 172
PspN4I GGNNCC 7 cut(s) 122, 178, 370, 687, 785, 867, 957
PspPI GGNCC 5 cut(s) 96, 530, 784, 865, 980
PstI CTGCAG 1 cut(s) 729
PsuI RGATCY 1 cut(s) 551
PvuII CAGCTG 1 cut(s) 986
RsaI GTAC 2 cut(s) 605, 881
RsaNI GTAC 2 cut(s) 604, 880
SalI GTCGAC 1 cut(s) 249
SaqAI TTAA 5 cut(s) 21, 240, 498, 734, 770
SatI GCNGC 6 cut(s) 188, 301, 689, 715, 987, 1008
Sau3AI GATC 1 cut(s) 551
Sau96I GGNCC 5 cut(s) 96, 530, 784, 865, 980
ScrFI CCNGG 4 cut(s) 62, 174, 544, 968
SduI GDGCHC 2 cut(s) 459, 489
SexAI ACCWGGT 1 cut(s) 172
SfaNI GCATC 3 cut(s) 17, 204, 882
SfcI CTRYAG 1 cut(s) 725
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 4 cut(s) 96, 530, 865, 980
SlaI CTCGAG 1 cut(s) 38
SmlI CTYRAG 4 cut(s) 38, 257, 395, 769
SmoI CTYRAG 4 cut(s) 38, 257, 395, 769
Sse9I AATT 6 cut(s) 137, 263, 400, 460, 950, 990
SsiI CCGC 4 cut(s) 188, 504, 689, 1007
SspMI CTAG 2 cut(s) 159, 518
StyD4I CCNGG 4 cut(s) 60, 172, 542, 966
StyI CCWWGG 2 cut(s) 27, 976
TaaI ACNGT 7 cut(s) 105, 148, 373, 491, 530, 721, 937
TaiI ACGT 4 cut(s) 90, 345, 426, 561
TaqI TCGA 3 cut(s) 39, 112, 250
TaqII GACCGA 1 cut(s) 80
TasI AATT 6 cut(s) 137, 263, 400, 460, 950, 990
TatI WGTACW 1 cut(s) 603
TauI GCSGC 3 cut(s) 190, 691, 1010
TfiI GAWTC 5 cut(s) 57, 77, 224, 385, 799
Tru1I TTAA 5 cut(s) 21, 240, 498, 734, 770
Tru9I TTAA 5 cut(s) 21, 240, 498, 734, 770
TscAI CASTG 4 cut(s) 494, 646, 724, 769
TseFI GTSAC 3 cut(s) 307, 441, 491
TseI GCWGC 3 cut(s) 300, 714, 986
Tsp45I GTSAC 3 cut(s) 307, 441, 491
TspDTI ATGAA 4 cut(s) 429, 458, 629, 847
TspRI CASTG 4 cut(s) 494, 646, 724, 769
Van91I CCANNNNNTGG 1 cut(s) 105
Vha464I CTTAAG 1 cut(s) 769
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 4 cut(s) 96, 530, 865, 980
XapI RAATTY 2 cut(s) 263, 400
XhoI CTCGAG 1 cut(s) 38
XmiI GTMKAC 2 cut(s) 250, 434
XmnI GAANNNNTTC 3 cut(s) 854, 905, 954
XspI CTAG 2 cut(s) 159, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.