Prupe.3G023500_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001298
Physical Location & Seq
Reverse (-)
1737553 .. 1738686
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G023500.1

Sequence Viewer

Length: 1134 bp
ATGGGTTCTTTGAAGAGGAAAAGAGAAACCCAGAAGGAAGACGAACAAGGGCTTTTAGAGAAGAGTTTTTTACTTACTGGGTGTGGCTCGTGGGTTCGAGGGAGGAAGCTCGGCCAAGGCGGATTTGGGTCCGTTTATTTGGCCTCTGTGAAAAAACCCAAATTGGGTAGCGAGGGTTTTCCGGCGATCATGGCGGTGAAATCGGCATTGATGGTTGAATCGCTTGAGCTGATGATGGAAAAGTCACTTCTTCAAACGCTTCGTAGCAGCCCTTTTGTTATCAACTGTTATGGAGAAGATGTGACGGCTGGGTTTGGTCATAATTTGGTATACAACGTCTTCTTGGAGTATGCTGATGGAGGAACAGTGGGGGATTTGATAAAGCAATCTGGGGGCTCTGGGCTCTGTGAATTACAAGTGAGGAAATATACAGAGTCTATTTTGAAAGGGGTTCAGTACATTCATGAAATGGGTTTTGTGCACTGTGATTTGAAGCCTGAAAACATCTTACTTGTGACCTCTGGTTCTGGTTTTGTGCCCAAAATTGGGGACTTGGGACTGGCAAAGAGGGCTGTGAGCAAGCGTTCTTGTAGAGGCACTCCAATGTACTTATCTCCTGAAACTGTGCTCGATAGAATTCAGGAGAAGCCTTCTGATATTTGGGCATTGGGCTGTGTTGTGCTGAAGATGCTCACTGGGAGGCATCCATGGGATGCAGAAGCCGCCGTGAAACTTCATGACCTAAAGCCTCAGATTGCTTCTGGAGTTCCTAACATTCCTGGTGGGTTATCAGAAGAGGCGAAAGATTTCTTAAAGAATTGCTTTATGAGGAACCCTTCAGAGAGACTCACAGCAGCTAAGCTATTGCACCACCCCTTTGTGACGAAACTTGATGAGATTGGACAGGTTAAAGTTGAACCAATGAAGGAAGTTTCTTCAGTGTCATCTTCTGAGCACTGTGTTCTTGATTATGGTAGTTTCATACCACTTGGAAGTTGGAGCTCTGAAGAGGCAGAGGAGATGGAGCAACAGATTCTTCCTTTGGCCCTCATGTCCCCTAGAGATTCAACCCCTGTGATCCCAACCACTGGGTGCCAAAAGCCATCAGCTTTTCCTATAATGGGTGCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

378

Amino Acids

41.06

Weight (kDa)

6.72

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1092
AccB7I CCANNNNNTGG 1 cut(s) 1088
AccI GTMKAC 1 cut(s) 330
AciI CCGC 3 cut(s) 120, 194, 723
AclWI GGATC 1 cut(s) 1072
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 1 cut(s) 636
AcuI CTGAAG 4 cut(s) 704, 822, 921, 1026
AdeI CACNNNGTG 1 cut(s) 1092
AfaI GTAC 2 cut(s) 458, 608
AfiI CCNNNNNNNGG 5 cut(s) 164, 545, 546, 1088, 1121
AgsI TTSAA 7 cut(s) 13, 218, 254, 445, 493, 917, 1068
AjnI CCWGG 1 cut(s) 778
AluBI AGCT 7 cut(s) 109, 229, 857, 862, 1002, 1109, 1130
AluI AGCT 7 cut(s) 109, 229, 857, 862, 1002, 1109, 1130
Alw21I GWGCWC 4 cut(s) 483, 630, 957, 1004
Alw26I GTCTC 1 cut(s) 838
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 1 cut(s) 1072
AoxI GGCC 3 cut(s) 112, 141, 1044
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 3 cut(s) 267, 854, 1127
ApoI RAATTY 1 cut(s) 636
Asp700I GAANNNNTTC 1 cut(s) 806
AspS9I GGNCC 2 cut(s) 129, 1045
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 129
BaeGI GKGCMC 2 cut(s) 483, 540
BanI GGYRCC 1 cut(s) 1092
BanII GRGCYC 3 cut(s) 398, 405, 1004
BauI CACGAG 1 cut(s) 88
BbsI GAAGAC 2 cut(s) 45, 331
Bbv12I GWGCWC 4 cut(s) 483, 630, 957, 1004
BbvI GCAGC 2 cut(s) 279, 866
BccI CCATC 5 cut(s) 205, 229, 350, 1015, 1111
BceAI ACGGC 2 cut(s) 321, 710
BciT130I CCWGG 1 cut(s) 780
BcoDI GTCTC 1 cut(s) 838
BfaI CTAG 1 cut(s) 1059
BisI GCNGC 4 cut(s) 268, 723, 855, 1128
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 4 cut(s) 269, 724, 856, 1129
Bme1390I CCNGG 1 cut(s) 780
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 129, 1045
BmiI GGNNCC 3 cut(s) 130, 833, 1094
BmrFI CCNGG 1 cut(s) 780
BmrI ACTGGG 3 cut(s) 87, 705, 1098
BmsI GCATC 3 cut(s) 678, 703, 712
BmuI ACTGGG 3 cut(s) 87, 705, 1098
BpiI GAAGAC 2 cut(s) 45, 331
BpmI CTGGAG 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 115, 707
BsaXI ACNNNNNCTCC 4 cut(s) 285, 315, 351, 381
Bsc4I CCNNNNNNNGG 5 cut(s) 164, 545, 546, 1088, 1121
Bse1I ACTGG 4 cut(s) 82, 564, 700, 1093
BseBI CCWGG 1 cut(s) 780
BseDI CCNNGG 2 cut(s) 115, 707
BseGI GGATG 2 cut(s) 703, 718
BseLI CCNNNNNNNGG 5 cut(s) 164, 545, 546, 1088, 1121
BseMII CTCAG 2 cut(s) 764, 942
BseNI ACTGG 4 cut(s) 82, 564, 700, 1093
BseRI GAGGAG 1 cut(s) 1031
BseSI GKGCMC 2 cut(s) 483, 540
BseXI GCAGC 2 cut(s) 279, 866
BseYI CCCAGC 1 cut(s) 308
BshFI GGCC 3 cut(s) 114, 143, 1046
BshNI GGYRCC 1 cut(s) 1092
BsiHKAI GWGCWC 4 cut(s) 483, 630, 957, 1004
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 3 cut(s) 563, 570, 1039
BslI CCNNNNNNNGG 5 cut(s) 164, 545, 546, 1088, 1121
BsmAI GTCTC 1 cut(s) 838
BsmFI GGGAC 3 cut(s) 563, 570, 1039
BsnI GGCC 3 cut(s) 114, 143, 1046
Bsp1286I GDGCHC 7 cut(s) 398, 405, 483, 540, 630, 957, 1004
Bsp143I GATC 2 cut(s) 186, 1077
Bsp1720I GCTNAGC 1 cut(s) 858
Bsp19I CCATGG 1 cut(s) 707
BspACI CCGC 3 cut(s) 120, 194, 723
BspANI GGCC 3 cut(s) 114, 143, 1046
BspCNI CTCAG 2 cut(s) 763, 943
BspHI TCATGA 2 cut(s) 463, 736
BspLI GGNNCC 3 cut(s) 130, 833, 1094
BspPI GGATC 1 cut(s) 1072
BspT107I GGYRCC 1 cut(s) 1092
BsrI ACTGG 4 cut(s) 82, 564, 700, 1093
BssECI CCNNGG 2 cut(s) 115, 707
BssMI GATC 2 cut(s) 186, 1077
BssNAI GTATAC 1 cut(s) 331
BssSI CACGAG 1 cut(s) 88
BssT1I CCWWGG 2 cut(s) 115, 707
Bst1107I GTATAC 1 cut(s) 331
Bst2BI CACGAG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 5 cut(s) 287, 367, 485, 625, 959
Bst6I CTCTTC 4 cut(s) 8, 56, 789, 1002
BstC8I GCNNGC 1 cut(s) 581
BstDEI CTNAG 4 cut(s) 750, 858, 951, 1131
BstDSI CCRYGG 1 cut(s) 707
BstF5I GGATG 2 cut(s) 703, 718
BstKTI GATC 2 cut(s) 189, 1080
BstMAI GTCTC 1 cut(s) 838
BstMBI GATC 2 cut(s) 186, 1077
BstMWI GCNNNNNNNGC 4 cut(s) 191, 569, 688, 722
BstNI CCWGG 1 cut(s) 780
BstSCI CCNGG 1 cut(s) 778
BstSLI GKGCMC 2 cut(s) 483, 540
BstV1I GCAGC 2 cut(s) 279, 866
BstV2I GAAGAC 2 cut(s) 45, 331
BstZ17I GTATAC 1 cut(s) 331
BsuRI GGCC 3 cut(s) 114, 143, 1046
BtgI CCRYGG 1 cut(s) 707
BtsCI GGATG 2 cut(s) 703, 718
BtsIMutI CAGTG 6 cut(s) 372, 481, 693, 945, 955, 1086
Cac8I GCNNGC 1 cut(s) 581
CciI TCATGA 2 cut(s) 463, 736
Cfr13I GGNCC 2 cut(s) 129, 1045
Csp6I GTAC 2 cut(s) 457, 607
CviAII CATG 5 cut(s) 190, 464, 708, 737, 1051
CviQI GTAC 2 cut(s) 457, 607
DdeI CTNAG 4 cut(s) 750, 858, 951, 1131
DpnI GATC 2 cut(s) 188, 1079
DpnII GATC 2 cut(s) 186, 1077
DraIII CACNNNGTG 1 cut(s) 1092
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 4 cut(s) 8, 56, 789, 1002
EarI CTCTTC 4 cut(s) 8, 56, 789, 1002
EciI GGCGGA 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 1002
Eco130I CCWWGG 2 cut(s) 115, 707
Eco24I GRGCYC 3 cut(s) 398, 405, 1004
Eco47I GGWCC 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 1002
Eco57I CTGAAG 4 cut(s) 704, 822, 921, 1026
EcoICRI GAGCTC 1 cut(s) 1002
EcoRI GAATTC 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 778
EcoT14I CCWWGG 2 cut(s) 115, 707
EcoT38I GRGCYC 3 cut(s) 398, 405, 1004
ErhI CCWWGG 2 cut(s) 115, 707
FaeI CATG 5 cut(s) 193, 467, 711, 740, 1054
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FaqI GGGAC 3 cut(s) 563, 570, 1039
FatI CATG 5 cut(s) 189, 463, 707, 736, 1050
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 4 cut(s) 268, 723, 855, 1128
FokI GGATG 2 cut(s) 690, 725
FriOI GRGCYC 3 cut(s) 398, 405, 1004
Fsp4HI GCNGC 4 cut(s) 268, 723, 855, 1128
FspBI CTAG 1 cut(s) 1059
GluI GCNGC 4 cut(s) 268, 723, 855, 1128
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 3 cut(s) 114, 143, 1046
HapII CCGG 1 cut(s) 182
Hin1II CATG 5 cut(s) 193, 467, 711, 740, 1054
HinfI GANTC 5 cut(s) 218, 434, 846, 1033, 1064
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 2 cut(s) 331, 481
Hpy188I TCNGA 6 cut(s) 655, 753, 793, 841, 952, 1006
Hpy188III TCNNGA 6 cut(s) 464, 617, 641, 737, 762, 965
Hpy8I GTNNAC 2 cut(s) 331, 481
HpyAV CCTTC 4 cut(s) 28, 660, 846, 919
HpyCH4III ACNGT 5 cut(s) 287, 367, 485, 625, 959
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 4 cut(s) 481, 716, 868, 1127
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 569, 688, 722
HpyF3I CTNAG 4 cut(s) 750, 858, 951, 1131
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 5 cut(s) 193, 467, 711, 740, 1054
Kzo9I GATC 2 cut(s) 186, 1077
LmnI GCTCC 2 cut(s) 999, 1024
Lsp1109I GCAGC 2 cut(s) 279, 866
LweI GCATC 3 cut(s) 678, 703, 712
MaeI CTAG 1 cut(s) 1059
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 4 cut(s) 243, 301, 514, 880
MalI GATC 2 cut(s) 188, 1079
MboI GATC 2 cut(s) 186, 1077
MhlI GDGCHC 7 cut(s) 398, 405, 483, 540, 630, 957, 1004
MluCI AATT 6 cut(s) 161, 322, 410, 543, 636, 817
MlyI GAGTC 2 cut(s) 443, 840
MmeI TCCRAC 1 cut(s) 977
MroXI GAANNNNTTC 1 cut(s) 806
MseI TTAA 2 cut(s) 812, 909
MslI CAYNNNNRTG 2 cut(s) 194, 602
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 780
MvaI CCWGG 1 cut(s) 780
MwoI GCNNNNNNNGC 4 cut(s) 191, 569, 688, 722
NcoI CCATGG 1 cut(s) 707
NdeII GATC 2 cut(s) 186, 1077
NlaIII CATG 5 cut(s) 193, 467, 711, 740, 1054
NlaIV GGNNCC 3 cut(s) 130, 833, 1094
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 4 cut(s) 243, 301, 514, 880
PagI TCATGA 2 cut(s) 463, 736
PdmI GAANNNNTTC 1 cut(s) 806
PfeI GAWTC 3 cut(s) 218, 1033, 1064
PflMI CCANNNNNTGG 1 cut(s) 1088
PkrI GCNGC 4 cut(s) 269, 724, 856, 1129
PleI GAGTC 2 cut(s) 442, 840
PpsI GAGTC 2 cut(s) 442, 840
Psp124BI GAGCTC 1 cut(s) 1004
Psp6I CCWGG 1 cut(s) 778
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 1 cut(s) 778
PspN4I GGNNCC 3 cut(s) 130, 833, 1094
PspPI GGNCC 2 cut(s) 129, 1045
RsaI GTAC 2 cut(s) 458, 608
RsaNI GTAC 2 cut(s) 457, 607
RseI CAYNNNNRTG 2 cut(s) 194, 602
SacI GAGCTC 1 cut(s) 1004
SaqAI TTAA 2 cut(s) 812, 909
SatI GCNGC 4 cut(s) 268, 723, 855, 1128
Sau3AI GATC 2 cut(s) 186, 1077
Sau96I GGNCC 2 cut(s) 129, 1045
SchI GAGTC 2 cut(s) 443, 840
ScrFI CCNGG 1 cut(s) 780
SduI GDGCHC 7 cut(s) 398, 405, 483, 540, 630, 957, 1004
SfaNI GCATC 3 cut(s) 678, 703, 712
SinI GGWCC 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 194, 602
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 6 cut(s) 161, 322, 410, 543, 636, 817
SsiI CCGC 3 cut(s) 120, 194, 723
SspMI CTAG 1 cut(s) 1059
SstI GAGCTC 1 cut(s) 1004
StyD4I CCNGG 1 cut(s) 778
StyI CCWWGG 2 cut(s) 115, 707
TaaI ACNGT 5 cut(s) 287, 367, 485, 625, 959
TaiI ACGT 1 cut(s) 339
TaqI TCGA 2 cut(s) 97, 630
TasI AATT 6 cut(s) 161, 322, 410, 543, 636, 817
TatI WGTACW 2 cut(s) 456, 606
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 3 cut(s) 218, 1033, 1064
Tru1I TTAA 2 cut(s) 812, 909
Tru9I TTAA 2 cut(s) 812, 909
TscAI CASTG 6 cut(s) 372, 488, 700, 945, 962, 1093
TseFI GTSAC 4 cut(s) 243, 301, 514, 880
TseI GCWGC 3 cut(s) 267, 854, 1127
Tsp45I GTSAC 4 cut(s) 243, 301, 514, 880
TspDTI ATGAA 5 cut(s) 452, 480, 725, 938, 970
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 6 cut(s) 372, 488, 700, 945, 962, 1093
Van91I CCANNNNNTGG 1 cut(s) 1088
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 129
XapI RAATTY 1 cut(s) 636
XcmI CCANNNNNNNNNTGG 2 cut(s) 122, 993
XmiI GTMKAC 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 806
XspI CTAG 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.