Rroxscaffold_2G00098230

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001298
Physical Location & Seq
Reverse (-)
19414716 .. 19423254
8539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098230.1

Sequence Viewer

Length: 999 bp
ATGGCGAGCCTTCTTCAACAATATGCATGTTCTATTGGTCTATGTTTACGCAAACGGCCTGGCCTCATTCCGGACAAATGCATGTCAGTGATGAGTGAATCAGACTATTTTAAGGCCATGGAAAAGCTCGAGAAGAAGAGGAAAAGAATCCAGGAGGAAGAGCCGGATTCTAGCAACGTCGGTCGGACAAAACGGTGGGTTCGACAAAGGTTCCTCGGCGCAGGACAATTCGGGACGGTTTTCTTGGCTCGTCCGGCCAAACCAGGTTCCAAACTGCCGCCGGTTATGGCCGTGAAGTCAGCGATGCTCTCGGAATCTGCCGACTTGGTTAAAGAAAGTCGACTTCTCAAGAAATTCAAGAACTGCCCTTTTATCCTTGACACTTACGGCAGCGATGTGACTAAAGACGTTGAAGGTGAGGAGTGGTTCAACGTTTTCTTGGAGTATGCTCAAAGAGGAACCACAGAGGATTGGATTCAGAAACTCAAGAATTTCGGGTTGCATGAGCTGCACGGGCTCAAGTACATTCATGGAAAGGGTTTTGTGCACTGTGACATTAAGCCCGCCAATGTATTGCTAGTGCGTGACGGCCCTGATGATACCGGGTTTGGATCTTACGTGGCGAAAATCGGGGATTTAGGGCTGGCAAAGAGGGCTGACAAGTACAATGTGATGAAGCGTTCTGATTATATAAATGGCACTGAGTTGTATATGTCTCCAGAAGTATCGCTTCACAACATTCAGGAGCCGCCAGCCGACATCTGGGCATTAGGCTGCACTATTCTGCAGATGTTAACCGGGATGGAGTTTTGGGATGTGAACACTGACTTAAGAGATGACTGGGGAATTGGTCAGCCGCACCACGTGGCGGTGCAGCTGCCCCACGCAAGAACCTCTCTACATTGTAGAATCGCTGTAAATCTGCTATTCATTAAGCAACTTGGCGAGGCCGGGAGGGTGGCCTCCGGTGTAGTCTTGGCCGACTTCCACCATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

332

Amino Acids

37.38

Weight (kDa)

8.55

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 68 - 276 2.7e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 69 - 266 5.7e-17 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 340
AccIII TCCGGA 1 cut(s) 70
AciI CCGC 5 cut(s) 278, 564, 749, 857, 869
AclI AACGTT 1 cut(s) 432
AclWI GGATC 1 cut(s) 619
AcoI YGGCCR 3 cut(s) 255, 288, 978
AcsI RAATTY 2 cut(s) 353, 490
AcvI CACGTG 1 cut(s) 865
AdeI CACNNNGTG 1 cut(s) 865
AfaI GTAC 2 cut(s) 524, 665
AfiI CCNNNNNNNGG 3 cut(s) 70, 762, 868
AflII CTTAAG 1 cut(s) 829
AgsI TTSAA 4 cut(s) 17, 358, 413, 430
AjnI CCWGG 3 cut(s) 58, 150, 262
AluBI AGCT 3 cut(s) 127, 508, 877
AluI AGCT 3 cut(s) 127, 508, 877
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 1 cut(s) 720
Alw44I GTGCAC 1 cut(s) 545
AlwI GGATC 1 cut(s) 619
Ama87I CYCGRG 1 cut(s) 128
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 9 cut(s) 56, 61, 114, 255, 288, 589, 948, 960, 978
ApaLI GTGCAC 1 cut(s) 545
ApeKI GCWGC 5 cut(s) 390, 508, 774, 874, 877
ApoI RAATTY 2 cut(s) 353, 490
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 1 cut(s) 590
AsuC2I CCSGG 3 cut(s) 604, 799, 952
AsuHPI GGTGA 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 549
BanII GRGCYC 1 cut(s) 519
BbrPI CACGTG 1 cut(s) 865
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 5 cut(s) 402, 495, 761, 864, 886
BccI CCATC 1 cut(s) 796
BceAI ACGGC 4 cut(s) 71, 275, 403, 604
BciT130I CCWGG 3 cut(s) 60, 152, 264
BcnI CCSGG 3 cut(s) 604, 799, 952
BcoDI GTCTC 1 cut(s) 720
BfaI CTAG 2 cut(s) 171, 578
BfmI CTRYAG 1 cut(s) 785
BfrI CTTAAG 1 cut(s) 829
BisI GCNGC 8 cut(s) 278, 391, 509, 749, 775, 857, 875, 878
BlsI GCNGC 8 cut(s) 279, 392, 510, 750, 776, 858, 876, 879
Bme1390I CCNGG 6 cut(s) 60, 152, 264, 604, 799, 952
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 4 cut(s) 212, 268, 460, 747
BmrFI CCNGG 6 cut(s) 60, 152, 264, 604, 799, 952
BmrI ACTGGG 1 cut(s) 850
BmsI GCATC 1 cut(s) 294
BmuI ACTGGG 1 cut(s) 850
BpmI CTGGAG 1 cut(s) 702
BpuEI CTTGAG 3 cut(s) 332, 470, 503
BpuMI CCSGG 3 cut(s) 604, 799, 952
BsaAI YACGTR 2 cut(s) 619, 865
BsaJI CCNNGG 2 cut(s) 117, 214
BsaWI WCCGGW 2 cut(s) 70, 965
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 762, 868
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 845
BseAI TCCGGA 1 cut(s) 70
BseBI CCWGG 3 cut(s) 60, 152, 264
BseDI CCNNGG 2 cut(s) 117, 214
BseGI GGATG 2 cut(s) 807, 820
BseLI CCNNNNNNNGG 3 cut(s) 70, 762, 868
BseMII CTCAG 1 cut(s) 693
BseNI ACTGG 1 cut(s) 845
BseRI GAGGAG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 549
BseXI GCAGC 5 cut(s) 402, 495, 761, 864, 886
BsgI GTGCAG 3 cut(s) 494, 760, 893
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 9 cut(s) 58, 63, 116, 257, 290, 591, 950, 962, 980
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 8 cut(s) 71, 164, 254, 281, 603, 798, 951, 966
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 3 cut(s) 70, 762, 868
BsmAI GTCTC 1 cut(s) 720
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 9 cut(s) 58, 63, 116, 257, 290, 591, 950, 962, 980
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 519, 549
Bsp13I TCCGGA 1 cut(s) 70
Bsp143I GATC 1 cut(s) 611
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 5 cut(s) 278, 564, 749, 857, 869
BspANI GGCC 9 cut(s) 58, 63, 116, 257, 290, 591, 950, 962, 980
BspCNI CTCAG 1 cut(s) 694
BspEI TCCGGA 1 cut(s) 70
BspLI GGNNCC 4 cut(s) 212, 268, 460, 747
BspMAI CTGCAG 1 cut(s) 789
BspPI GGATC 1 cut(s) 619
BspQI GCTCTTC 1 cut(s) 153
BspTI CTTAAG 1 cut(s) 829
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 1 cut(s) 845
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 117, 214
BssMI GATC 1 cut(s) 611
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 3 cut(s) 60, 152, 264
Bst4CI ACNGT 3 cut(s) 195, 238, 551
Bst6I CTCTTC 2 cut(s) 131, 153
BstAFI CTTAAG 1 cut(s) 829
BstAPI GCANNNNNTGC 1 cut(s) 508
BstBAI YACGTR 2 cut(s) 619, 865
BstC8I GCNNGC 4 cut(s) 7, 564, 645, 753
BstDEI CTNAG 1 cut(s) 702
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 2 cut(s) 807, 820
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 1 cut(s) 614
BstMAI GTCTC 1 cut(s) 720
BstMBI GATC 1 cut(s) 611
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 4 cut(s) 254, 508, 514, 653
BstNI CCWGG 3 cut(s) 60, 152, 264
BstNSI RCATGY 2 cut(s) 30, 85
BstSCI CCNGG 6 cut(s) 58, 150, 262, 602, 797, 950
BstSFI CTRYAG 1 cut(s) 785
BstSLI GKGCMC 1 cut(s) 549
BstV1I GCAGC 5 cut(s) 402, 495, 761, 864, 886
BstX2I RGATCY 1 cut(s) 611
BstYI RGATCY 1 cut(s) 611
BsuRI GGCC 9 cut(s) 58, 63, 116, 257, 290, 591, 950, 962, 980
BtgI CCRYGG 1 cut(s) 117
BtgZI GCGATG 2 cut(s) 317, 408
BtsCI GGATG 2 cut(s) 807, 820
BtsIMutI CAGTG 4 cut(s) 93, 547, 699, 822
Cac8I GCNNGC 4 cut(s) 7, 564, 645, 753
CfoI GCGC 1 cut(s) 221
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 590
CsiI ACCWGGT 1 cut(s) 262
Csp6I GTAC 2 cut(s) 523, 664
CviAII CATG 5 cut(s) 27, 82, 118, 503, 530
CviQI GTAC 2 cut(s) 523, 664
DdeI CTNAG 1 cut(s) 702
DpnI GATC 1 cut(s) 613
DpnII GATC 1 cut(s) 611
DraIII CACNNNGTG 1 cut(s) 865
EaeI YGGCCR 3 cut(s) 255, 288, 978
Eam1104I CTCTTC 2 cut(s) 131, 153
EarI CTCTTC 2 cut(s) 131, 153
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 519
Eco72I CACGTG 1 cut(s) 865
Eco88I CYCGRG 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 58, 150, 262
EcoT14I CCWWGG 1 cut(s) 117
EcoT22I ATGCAT 2 cut(s) 28, 83
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 5 cut(s) 30, 85, 121, 506, 533
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 247
FatI CATG 5 cut(s) 26, 81, 117, 502, 529
FauI CCCGC 1 cut(s) 571
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 8 cut(s) 278, 391, 509, 749, 775, 857, 875, 878
FokI GGATG 2 cut(s) 814, 827
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 8 cut(s) 278, 391, 509, 749, 775, 857, 875, 878
FspBI CTAG 2 cut(s) 171, 578
GlaI GCGC 1 cut(s) 220
GluI GCNGC 8 cut(s) 278, 391, 509, 749, 775, 857, 875, 878
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 9 cut(s) 58, 63, 116, 257, 290, 591, 950, 962, 980
HapII CCGG 8 cut(s) 71, 164, 254, 281, 603, 798, 951, 966
HhaI GCGC 1 cut(s) 221
Hin1II CATG 5 cut(s) 30, 85, 121, 506, 533
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HincII GTYRAC 2 cut(s) 341, 795
HindII GTYRAC 2 cut(s) 341, 795
HinfI GANTC 6 cut(s) 98, 147, 167, 314, 475, 909
HpaI GTTAAC 1 cut(s) 795
HpaII CCGG 8 cut(s) 71, 164, 254, 281, 603, 798, 951, 966
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 5 cut(s) 47, 341, 547, 795, 820
Hpy188I TCNGA 5 cut(s) 103, 186, 313, 480, 685
Hpy188III TCNNGA 8 cut(s) 71, 130, 232, 349, 358, 487, 719, 743
Hpy8I GTNNAC 5 cut(s) 47, 341, 547, 795, 820
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 20, 407
HpyCH4III ACNGT 3 cut(s) 195, 238, 551
HpyCH4IV ACGT 5 cut(s) 177, 408, 432, 618, 864
HpyCH4V TGCA 8 cut(s) 26, 81, 502, 511, 547, 777, 787, 874
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 508, 514, 653
HpyF3I CTNAG 1 cut(s) 702
HpySE526I ACGT 5 cut(s) 177, 408, 432, 618, 864
Hsp92II CATG 5 cut(s) 30, 85, 121, 506, 533
HspAI GCGC 1 cut(s) 219
Kpn2I TCCGGA 1 cut(s) 70
KspAI GTTAAC 1 cut(s) 795
Kzo9I GATC 1 cut(s) 611
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 745
Lsp1109I GCAGC 5 cut(s) 402, 495, 761, 864, 886
LweI GCATC 1 cut(s) 294
MabI ACCWGGT 1 cut(s) 262
MaeI CTAG 2 cut(s) 171, 578
MaeII ACGT 5 cut(s) 177, 408, 432, 618, 864
MaeIII GTNAC 3 cut(s) 397, 551, 584
MalI GATC 1 cut(s) 613
MboI GATC 1 cut(s) 611
MboII GAAGA 4 cut(s) 5, 145, 148, 170
MflI RGATCY 1 cut(s) 611
MhlI GDGCHC 2 cut(s) 519, 549
MluCI AATT 4 cut(s) 227, 353, 490, 846
MmeI TCCRAC 1 cut(s) 164
Mph1103I ATGCAT 2 cut(s) 28, 83
MroI TCCGGA 1 cut(s) 70
MseI TTAA 6 cut(s) 111, 330, 558, 794, 830, 933
MslI CAYNNNNRTG 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 877
MspCI CTTAAG 1 cut(s) 829
MspI CCGG 8 cut(s) 71, 164, 254, 281, 603, 798, 951, 966
MspR9I CCNGG 6 cut(s) 60, 152, 264, 604, 799, 952
MvaI CCWGG 3 cut(s) 60, 152, 264
MwoI GCNNNNNNNGC 4 cut(s) 254, 508, 514, 653
NciI CCSGG 3 cut(s) 604, 799, 952
NcoI CCATGG 1 cut(s) 117
NdeII GATC 1 cut(s) 611
NlaIII CATG 5 cut(s) 30, 85, 121, 506, 533
NlaIV GGNNCC 4 cut(s) 212, 268, 460, 747
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 3 cut(s) 397, 551, 584
NsiI ATGCAT 2 cut(s) 28, 83
NspI RCATGY 2 cut(s) 30, 85
PaeR7I CTCGAG 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 199
PfeI GAWTC 6 cut(s) 98, 147, 167, 314, 475, 909
PfoI TCCNGGA 1 cut(s) 150
PkrI GCNGC 8 cut(s) 279, 392, 510, 750, 776, 858, 876, 879
PmaCI CACGTG 1 cut(s) 865
PmlI CACGTG 1 cut(s) 865
Ppu21I YACGTR 2 cut(s) 619, 865
Psp1406I AACGTT 1 cut(s) 432
Psp6I CCWGG 3 cut(s) 58, 150, 262
PspCI CACGTG 1 cut(s) 865
PspGI CCWGG 3 cut(s) 58, 150, 262
PspN4I GGNNCC 4 cut(s) 212, 268, 460, 747
PspPI GGNCC 1 cut(s) 590
PstI CTGCAG 1 cut(s) 789
PsuI RGATCY 1 cut(s) 611
PvuII CAGCTG 1 cut(s) 877
RsaI GTAC 2 cut(s) 524, 665
RsaNI GTAC 2 cut(s) 523, 664
RseI CAYNNNNRTG 1 cut(s) 86
SalI GTCGAC 1 cut(s) 339
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 6 cut(s) 111, 330, 558, 794, 830, 933
SatI GCNGC 8 cut(s) 278, 391, 509, 749, 775, 857, 875, 878
Sau3AI GATC 1 cut(s) 611
Sau96I GGNCC 1 cut(s) 590
ScrFI CCNGG 6 cut(s) 60, 152, 264, 604, 799, 952
SduI GDGCHC 2 cut(s) 519, 549
SexAI ACCWGGT 1 cut(s) 262
SfaNI GCATC 1 cut(s) 294
SfcI CTRYAG 1 cut(s) 785
Sfr274I CTCGAG 1 cut(s) 128
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 5 cut(s) 128, 347, 485, 518, 829
SmoI CTYRAG 5 cut(s) 128, 347, 485, 518, 829
Sse9I AATT 4 cut(s) 227, 353, 490, 846
SsiI CCGC 5 cut(s) 278, 564, 749, 857, 869
SspMI CTAG 2 cut(s) 171, 578
StyD4I CCNGG 6 cut(s) 58, 150, 262, 602, 797, 950
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 3 cut(s) 195, 238, 551
TaiI ACGT 5 cut(s) 180, 411, 435, 621, 867
TaqI TCGA 3 cut(s) 129, 202, 340
TaqII GACCGA 1 cut(s) 170
TasI AATT 4 cut(s) 227, 353, 490, 846
TatI WGTACW 2 cut(s) 522, 663
TauI GCSGC 3 cut(s) 280, 751, 859
TfiI GAWTC 6 cut(s) 98, 147, 167, 314, 475, 909
Tru1I TTAA 6 cut(s) 111, 330, 558, 794, 830, 933
Tru9I TTAA 6 cut(s) 111, 330, 558, 794, 830, 933
TscAI CASTG 4 cut(s) 93, 554, 706, 829
TseFI GTSAC 3 cut(s) 397, 551, 584
TseI GCWGC 5 cut(s) 390, 508, 774, 874, 877
Tsp45I GTSAC 3 cut(s) 397, 551, 584
TspDTI ATGAA 3 cut(s) 518, 689, 919
TspRI CASTG 4 cut(s) 93, 554, 706, 829
Vha464I CTTAAG 1 cut(s) 829
VneI GTGCAC 1 cut(s) 545
XapI RAATTY 2 cut(s) 353, 490
XceI RCATGY 2 cut(s) 30, 85
XcmI CCANNNNNNNNNTGG 1 cut(s) 759
XhoI CTCGAG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 340
XspI CTAG 2 cut(s) 171, 578
Zsp2I ATGCAT 2 cut(s) 28, 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.