Prupe.3G111500_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001298
Physical Location & Seq
Reverse (-)
9287029 .. 9288210
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G111500.1

Sequence Viewer

Length: 1182 bp
ATGGCGGGTCTCAAGAGAAAAAGAGCAACCCCTGATGAGATAATAGTTGTAGACGATTCTGCAGATGATGCAGATCATTACTTGACAATTTCACTAAGTGGCTGCGAGTGGGTTCGAGGGCCGATGCTCGGCCAAGGCGGATTTGGGTCAGTTTATTTGGGTTTCGTGAAAAAACCCAGGTTGTGTTTGAAGGGTGTGCCGAGGATTGTGGCCGTGAAATCGACATTGGCCTCTCAGGCGCATGATCTGATGGGGGAAGATATTCTTCTTTACATTTTTCGTCACTGCCCTTTTGTTATCAACTGCTATGGAGAGGATGAGACGATTGGCGCTGATGGCCGTCATTTATTCAATGTGTTCTTGGAGTACGCGGATGGAGGAACAATGGCGGATTTGATTGAGAAATCAAGGGGTTTTGGGTTGCGTGAATTCCAAGTCAGAAAGTATACTGAGTCGATTTTGAAAGGGATTAAGTACATCCACGAAAGGGGTTTTGTGCATTGTGATCTCAAGCCTGAGAATATTCTGCTTGTGTCTGATACCGCTGGTTCCTCTGGTGGTGCTCGTTTTGTGCCGAAAATCGGAGACTTGGGGCTCACAAAGAAGGCAAATCAGAAGTGGGAGAAGCCTTCCTTTGGAGGCACACCAATGTATTTGTCTCCAGAAGCTGTGGTTTATGGAATTCAACAGCAGCCTTCTGATATTTGGGCGTTAGGTTGTGTTGTGCTGCAAATGTTAACTGGGAGGCATCCATGGGATTTCACACCTGGCGCCGTATTTGACGTTCGGGGCCTGTTAACTGCCCTGCTTGCTTCAAGAATTCCCGCCATTCCTGGTTGGTTATCAAAGGAGGCTAAAGATTTCTTGCAATGTTGCTTTATGTGGAACACTTCAGAGAGGTTTACTGCTGCCATGCTCTTGAACCACCCATTTGTGACTAAACTGGATAATGGGGTTGGAGAGGTTAAACCAATTGAGCAAGTGTCTTCTGCAGTGGCTTCCTCTGTTCTGGATTGTCCTAGCTTCATACCCCTTGGAAGTTGGAAGTCTGAAGATGCAGAGGAGATGGCTCAAGAAAATGTTGGTTTTTCTGAGCAGGAGATTCTTCCTCTGAAGCTCATGTCAAGGCATGTGGTTCCAAGCCCAAAACCATCAACTTTTGCTATAATTGGTGCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

394

Amino Acids

43.12

Weight (kDa)

6.02

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 770
AccI GTMKAC 2 cut(s) 51, 446
AccII CGCG 1 cut(s) 371
AciI CCGC 6 cut(s) 5, 138, 371, 389, 543, 825
AcoI YGGCCR 3 cut(s) 130, 210, 337
AcsI RAATTY 3 cut(s) 428, 681, 819
AcuI CTGAAG 3 cut(s) 876, 1071, 1133
AcyI GRCGYC 1 cut(s) 771
AdeI CACNNNGTG 1 cut(s) 98
AfaI GTAC 2 cut(s) 368, 476
AfiI CCNNNNNNNGG 4 cut(s) 128, 487, 581, 635
AgsI TTSAA 6 cut(s) 190, 352, 463, 686, 816, 922
AjnI CCWGG 3 cut(s) 176, 766, 832
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 4 cut(s) 668, 1023, 1117, 1178
AluI AGCT 4 cut(s) 668, 1023, 1117, 1178
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 4 cut(s) 14, 314, 579, 663
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 6 cut(s) 119, 130, 210, 228, 337, 790
ApeKI GCWGC 5 cut(s) 102, 691, 727, 908, 1175
ApoI RAATTY 3 cut(s) 428, 681, 819
Asp700I GAANNNNTTC 1 cut(s) 261
AspLEI GCGC 3 cut(s) 241, 332, 773
AspS9I GGNCC 2 cut(s) 119, 790
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 1 cut(s) 597
BbsI GAAGAC 1 cut(s) 978
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 4 cut(s) 89, 703, 714, 895
BccI CCATC 5 cut(s) 244, 329, 368, 1060, 1159
BceAI ACGGC 3 cut(s) 197, 324, 758
BciT130I CCWGG 3 cut(s) 178, 768, 834
BcoDI GTCTC 4 cut(s) 14, 314, 579, 663
BfaI CTAG 1 cut(s) 1020
BfmI CTRYAG 2 cut(s) 60, 990
BfoI RGCGCY 2 cut(s) 333, 774
BglI GCCNNNNNGGC 1 cut(s) 236
BisI GCNGC 5 cut(s) 103, 692, 728, 909, 1176
BlsI GCNGC 5 cut(s) 104, 693, 729, 910, 1177
Bme1390I CCNGG 3 cut(s) 178, 768, 834
BmgT120I GGNCC 2 cut(s) 119, 790
BmiI GGNNCC 4 cut(s) 550, 772, 791, 1137
BmrFI CCNGG 3 cut(s) 178, 768, 834
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 4 cut(s) 58, 114, 757, 1045
BmuI ACTGGG 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 978
BpmI CTGGAG 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 494, 1056
BsaBI GATNNNNATC 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 771
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 5 cut(s) 133, 176, 200, 752, 1033
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 487, 581, 635
Bse1I ACTGG 2 cut(s) 745, 948
Bse3DI GCAATG 1 cut(s) 875
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 3 cut(s) 178, 768, 834
BseDI CCNNGG 5 cut(s) 133, 176, 200, 752, 1033
BseGI GGATG 4 cut(s) 322, 379, 477, 748
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 4 cut(s) 128, 487, 581, 635
BseMI GCAATG 1 cut(s) 875
BseMII CTCAG 4 cut(s) 248, 441, 507, 1083
BseNI ACTGG 2 cut(s) 745, 948
BseRI GAGGAG 1 cut(s) 1076
BseXI GCAGC 4 cut(s) 89, 703, 714, 895
Bsh1236I CGCG 1 cut(s) 371
BshFI GGCC 6 cut(s) 121, 132, 212, 230, 339, 792
BshNI GGYRCC 1 cut(s) 770
BsiHKAI GWGCWC 1 cut(s) 565
BslI CCNNNNNNNGG 4 cut(s) 128, 487, 581, 635
BsmAI GTCTC 4 cut(s) 14, 314, 579, 663
BsmBI CGTCTC 1 cut(s) 314
BsnI GGCC 6 cut(s) 121, 132, 212, 230, 339, 792
Bso31I GGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 565, 597
Bsp143I GATC 3 cut(s) 73, 244, 505
Bsp19I CCATGG 1 cut(s) 752
BspACI CCGC 6 cut(s) 5, 138, 371, 389, 543, 825
BspANI GGCC 6 cut(s) 121, 132, 212, 230, 339, 792
BspCNI CTCAG 4 cut(s) 247, 442, 508, 1084
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 4 cut(s) 550, 772, 791, 1137
BspMAI CTGCAG 2 cut(s) 64, 994
BspT107I GGYRCC 1 cut(s) 770
BspTNI GGTCTC 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 875
BsrI ACTGG 2 cut(s) 745, 948
BssECI CCNNGG 5 cut(s) 133, 176, 200, 752, 1033
BssMI GATC 3 cut(s) 73, 244, 505
BssNAI GTATAC 1 cut(s) 447
BssNI GRCGYC 1 cut(s) 771
BssT1I CCWWGG 3 cut(s) 133, 752, 1033
Bst1107I GTATAC 1 cut(s) 447
Bst2UI CCWGG 3 cut(s) 178, 768, 834
BstACI GRCGYC 1 cut(s) 771
BstAPI GCANNNNNTGC 1 cut(s) 68
BstC8I GCNNGC 1 cut(s) 810
BstDEI CTNAG 6 cut(s) 95, 234, 450, 516, 1092, 1179
BstDSI CCRYGG 1 cut(s) 752
BstF5I GGATG 4 cut(s) 322, 379, 477, 748
BstFNI CGCG 1 cut(s) 371
BstH2I RGCGCY 2 cut(s) 333, 774
BstHHI GCGC 3 cut(s) 241, 332, 773
BstKTI GATC 3 cut(s) 76, 247, 508
BstMAI GTCTC 4 cut(s) 14, 314, 579, 663
BstMBI GATC 3 cut(s) 73, 244, 505
BstMWI GCNNNNNNNGC 4 cut(s) 68, 236, 336, 809
BstNI CCWGG 3 cut(s) 178, 768, 834
BstNSI RCATGY 1 cut(s) 1133
BstSCI CCNGG 3 cut(s) 176, 766, 832
BstSFI CTRYAG 2 cut(s) 60, 990
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 4 cut(s) 89, 703, 714, 895
BstV2I GAAGAC 1 cut(s) 978
BstZ17I GTATAC 1 cut(s) 447
BsuRI GGCC 6 cut(s) 121, 132, 212, 230, 339, 792
BtgI CCRYGG 1 cut(s) 752
BtsCI GGATG 4 cut(s) 322, 379, 477, 748
BtsI GCAGTG 2 cut(s) 283, 999
BtsIMutI CAGTG 2 cut(s) 283, 999
Cac8I GCNNGC 1 cut(s) 810
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 3 cut(s) 241, 332, 773
Cfr13I GGNCC 2 cut(s) 119, 790
Csp6I GTAC 2 cut(s) 367, 475
CspCI CAANNNNNGTGG 2 cut(s) 1113, 1148
CviAII CATG 5 cut(s) 242, 753, 913, 1120, 1130
CviQI GTAC 2 cut(s) 367, 475
DdeI CTNAG 6 cut(s) 95, 234, 450, 516, 1092, 1179
DinI GGCGCC 1 cut(s) 772
DpnI GATC 3 cut(s) 75, 246, 507
DpnII GATC 3 cut(s) 73, 244, 505
DraIII CACNNNGTG 1 cut(s) 98
EaeI YGGCCR 3 cut(s) 130, 210, 337
EciI GGCGGA 2 cut(s) 153, 404
Eco130I CCWWGG 3 cut(s) 133, 752, 1033
Eco24I GRGCYC 1 cut(s) 597
Eco31I GGTCTC 1 cut(s) 14
Eco57I CTGAAG 3 cut(s) 876, 1071, 1133
EcoO109I RGGNCCY 1 cut(s) 790
EcoRI GAATTC 3 cut(s) 428, 681, 819
EcoRII CCWGG 3 cut(s) 176, 766, 832
EcoT14I CCWWGG 3 cut(s) 133, 752, 1033
EcoT38I GRGCYC 1 cut(s) 597
EgeI GGCGCC 1 cut(s) 772
EheI GGCGCC 1 cut(s) 772
ErhI CCWWGG 3 cut(s) 133, 752, 1033
Esp3I CGTCTC 1 cut(s) 314
FaeI CATG 5 cut(s) 245, 756, 916, 1123, 1133
FalI AAGNNNNNCTT 4 cut(s) 249, 281, 617, 649
FatI CATG 5 cut(s) 241, 752, 912, 1119, 1129
FauI CCCGC 1 cut(s) 832
FblI GTMKAC 2 cut(s) 51, 446
Fnu4HI GCNGC 5 cut(s) 103, 692, 728, 909, 1176
FokI GGATG 4 cut(s) 329, 386, 464, 735
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 5 cut(s) 103, 692, 728, 909, 1176
FspBI CTAG 1 cut(s) 1020
GlaI GCGC 3 cut(s) 240, 331, 772
GluI GCNGC 5 cut(s) 103, 692, 728, 909, 1176
GsuI CTGGAG 1 cut(s) 645
HaeII RGCGCY 2 cut(s) 333, 774
HaeIII GGCC 6 cut(s) 121, 132, 212, 230, 339, 792
HhaI GCGC 3 cut(s) 241, 332, 773
Hin1I GRCGYC 1 cut(s) 771
Hin1II CATG 5 cut(s) 245, 756, 916, 1123, 1133
Hin6I GCGC 3 cut(s) 239, 330, 771
HinP1I GCGC 3 cut(s) 239, 330, 771
HincII GTYRAC 2 cut(s) 738, 798
HindII GTYRAC 2 cut(s) 738, 798
HinfI GANTC 3 cut(s) 56, 452, 1102
HpaI GTTAAC 2 cut(s) 738, 798
Hpy166II GTNNAC 5 cut(s) 52, 447, 738, 798, 903
Hpy188III TCNNGA 7 cut(s) 13, 166, 662, 816, 919, 1010, 1073
Hpy8I GTNNAC 5 cut(s) 52, 447, 738, 798, 903
HpyAV CCTTC 4 cut(s) 184, 598, 639, 705
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 8 cut(s) 62, 71, 499, 730, 868, 992, 1058, 1175
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 236, 336, 809
HpyF3I CTNAG 6 cut(s) 95, 234, 450, 516, 1092, 1179
HpySE526I ACGT 1 cut(s) 783
Hsp92I GRCGYC 1 cut(s) 771
Hsp92II CATG 5 cut(s) 245, 756, 916, 1123, 1133
HspAI GCGC 3 cut(s) 239, 330, 771
KasI GGCGCC 1 cut(s) 770
KspAI GTTAAC 2 cut(s) 738, 798
Kzo9I GATC 3 cut(s) 73, 244, 505
Lsp1109I GCAGC 4 cut(s) 89, 703, 714, 895
LweI GCATC 4 cut(s) 58, 114, 757, 1045
MaeI CTAG 1 cut(s) 1020
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 2 cut(s) 281, 934
MalI GATC 3 cut(s) 75, 246, 507
MboI GATC 3 cut(s) 73, 244, 505
MboII GAAGA 5 cut(s) 257, 269, 978, 1064, 1097
MfeI CAATTG 1 cut(s) 972
MhlI GDGCHC 2 cut(s) 565, 597
MluCI AATT 6 cut(s) 87, 428, 681, 819, 972, 1167
Mly113I GGCGCC 1 cut(s) 771
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 2 cut(s) 937, 1022
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 4 cut(s) 471, 737, 797, 966
MslI CAYNNNNRTG 1 cut(s) 647
MspA1I CMGCKG 1 cut(s) 545
MspR9I CCNGG 3 cut(s) 178, 768, 834
MunI CAATTG 1 cut(s) 972
MvaI CCWGG 3 cut(s) 178, 768, 834
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 4 cut(s) 68, 236, 336, 809
NarI GGCGCC 1 cut(s) 771
NcoI CCATGG 1 cut(s) 752
NdeII GATC 3 cut(s) 73, 244, 505
NlaIII CATG 5 cut(s) 245, 756, 916, 1123, 1133
NlaIV GGNNCC 4 cut(s) 550, 772, 791, 1137
NmeAIII GCCGAG 2 cut(s) 108, 225
NmuCI GTSAC 2 cut(s) 281, 934
NspI RCATGY 1 cut(s) 1133
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 56, 1102
PkrI GCNGC 5 cut(s) 104, 693, 729, 910, 1177
PleI GAGTC 1 cut(s) 460
PluTI GGCGCC 1 cut(s) 774
PpsI GAGTC 1 cut(s) 460
Psp6I CCWGG 3 cut(s) 176, 766, 832
PspGI CCWGG 3 cut(s) 176, 766, 832
PspN4I GGNNCC 4 cut(s) 550, 772, 791, 1137
PspPI GGNCC 2 cut(s) 119, 790
PsrI GAACNNNNNNTAC 2 cut(s) 768, 800
PstI CTGCAG 2 cut(s) 64, 994
PstNI CAGNNNCTG 1 cut(s) 668
RsaI GTAC 2 cut(s) 368, 476
RsaNI GTAC 2 cut(s) 367, 475
RseI CAYNNNNRTG 1 cut(s) 647
SaqAI TTAA 4 cut(s) 471, 737, 797, 966
SatI GCNGC 5 cut(s) 103, 692, 728, 909, 1176
Sau3AI GATC 3 cut(s) 73, 244, 505
Sau96I GGNCC 2 cut(s) 119, 790
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 3 cut(s) 178, 768, 834
SduI GDGCHC 2 cut(s) 565, 597
SfaNI GCATC 4 cut(s) 58, 114, 757, 1045
SfcI CTRYAG 2 cut(s) 60, 990
SfoI GGCGCC 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 3 cut(s) 11, 509, 1071
SmoI CTYRAG 3 cut(s) 11, 509, 1071
Sse9I AATT 6 cut(s) 87, 428, 681, 819, 972, 1167
SsiI CCGC 6 cut(s) 5, 138, 371, 389, 543, 825
SspDI GGCGCC 1 cut(s) 770
SspI AATATT 1 cut(s) 523
SspMI CTAG 1 cut(s) 1020
StyD4I CCNGG 3 cut(s) 176, 766, 832
StyI CCWWGG 3 cut(s) 133, 752, 1033
TaiI ACGT 1 cut(s) 786
TaqI TCGA 3 cut(s) 115, 221, 455
TasI AATT 6 cut(s) 87, 428, 681, 819, 972, 1167
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 2 cut(s) 56, 1102
Tru1I TTAA 4 cut(s) 471, 737, 797, 966
Tru9I TTAA 4 cut(s) 471, 737, 797, 966
TscAI CASTG 2 cut(s) 290, 999
TseFI GTSAC 2 cut(s) 281, 934
TseI GCWGC 5 cut(s) 102, 691, 727, 908, 1175
Tsp45I GTSAC 2 cut(s) 281, 934
TspDTI ATGAA 1 cut(s) 1015
TspRI CASTG 2 cut(s) 290, 999
XapI RAATTY 3 cut(s) 428, 681, 819
XceI RCATGY 1 cut(s) 1133
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XmiI GTMKAC 2 cut(s) 51, 446
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.