RLG00000008580

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001298
Physical Location & Seq
Reverse (-)
32498519 .. 32502472
3954 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008580

Sequence Viewer

Length: 1008 bp
ATGGCCGTTAAGGTCGCTGAGGTTTCTGATCCAAAAAAGGTTGATGCTTTATTCACCGAGGCCAAACTTCTCTCTCAGTTTCAGGACTGCCCTTTTGTTATCAACTCTTTTGGAGAACAGTGGACTTATAAGTCTCATCATAATTCTTTCTCTTTCAATCTGCTATTAGAATTTGCGGCCGGAGGCACTATAAGAGATAGGATTCAGAAATCCGCATCCGGCTTGTCCGAATGTGAGATAAAAGAGTTCACCAAGTCAGTTCTTAGTGGTCTCCAGTGCATTCATGACAAAGGTTATGTGCATTGCGACATAAAGCCCGATAACATTCTTCTTGTGCACGACATGACTTCTCATGTCGATTTTGTGGCTAAGATAGGAGACTTTGGATTGACAAAGAGCGCTCAAAATGAGTATTCTCGCCAAGGCACCGCAAGCTATTTGCCTCCTGAAACTGTTATCAGTAACATTCAAGGGAAGCCATCAGACATTTGGGCACTAGGATGTTTAGTTCTAGAGATGCTGACTGGAACCTTGTGGCCTTGCAAGACAAGTGAGGACTTAGGCGGTTGGGTTTCAAATCCAATTATTCCTTCACACTTGTCCGAGGAGGCCAAGGATTTTTTGGCAAAGTGTCTGGATATAGATGCTTCCAAGAGATTTACGGCTGAGAGGCTCTTGACTCACTCATTTCTTATGAGTGAACAGAGCGTTGAACAAGTTACTGTTGATGCAGCATGCGATCACAAAAGTGGGAAGTCTGGTGATGGAGTAGTGGTGCGAGATAAAGAAGGAAGGCTCAAGGGTGCAGTTCAACAGCCCATACCAGAGATTCGTAGCTATTACATGGAGAAAGATCCTTCAGAGATTGGAAACCAAGCTGCACTCATTCAGTACGTAGCAAGATCCTTCAGAGATTGGAAACCAAGCTGCACTCATTCAGTACGTAGCAAGGAAAGCTTTAGCCTCCGCTGGGACGAGATTTTCTGGAGGGAAGACCACCATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

336

Amino Acids

37.51

Weight (kDa)

5.75

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 231 5.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 2 - 226 2.7e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 129
AasI GACNNNNNNGTC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 425
AciI CCGC 5 cut(s) 176, 213, 429, 564, 967
AclWI GGATC 3 cut(s) 23, 848, 897
AcoI YGGCCR 2 cut(s) 3, 177
AcsI RAATTY 1 cut(s) 170
AcuI CTGAAG 2 cut(s) 843, 892
AfaI GTAC 2 cut(s) 893, 942
AfeI AGCGCT 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 970
AgsI TTSAA 5 cut(s) 157, 470, 576, 713, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 574, 606
AleI CACNNNNGTG 1 cut(s) 747
AloI GAACNNNNNNTCC 2 cut(s) 492, 524
AluBI AGCT 5 cut(s) 435, 837, 878, 927, 957
AluI AGCT 5 cut(s) 435, 837, 878, 927, 957
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 3 cut(s) 138, 275, 372
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 3 cut(s) 23, 848, 897
Aor51HI AGCGCT 1 cut(s) 400
AoxI GGCC 5 cut(s) 3, 60, 177, 536, 609
ApaLI GTGCAC 1 cut(s) 335
ApeKI GCWGC 3 cut(s) 731, 878, 927
ApoI RAATTY 1 cut(s) 170
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspLEI GCGC 1 cut(s) 401
AsuHPI GGTGA 3 cut(s) 46, 241, 773
BaeGI GKGCMC 2 cut(s) 339, 496
BanI GGYRCC 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 999
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 743, 865, 914
BccI CCATC 2 cut(s) 487, 758
BceAI ACGGC 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 138, 275, 372
BfaI CTAG 2 cut(s) 497, 512
BfoI RGCGCY 1 cut(s) 402
BisI GCNGC 4 cut(s) 177, 732, 879, 928
BlsI GCNGC 4 cut(s) 178, 733, 880, 929
BmiI GGNNCC 2 cut(s) 427, 529
BmsI GCATC 5 cut(s) 34, 224, 507, 634, 718
BpiI GAAGAC 1 cut(s) 999
BpmI CTGGAG 2 cut(s) 257, 1006
Bpu10I CCTNAGC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 782
BsaAI YACGTR 2 cut(s) 895, 944
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 4 cut(s) 57, 421, 603, 612
BsaXI ACNNNNNCTCC 2 cut(s) 759, 789
Bsc4I CCNNNNNNNGG 1 cut(s) 970
Bse1I ACTGG 2 cut(s) 274, 529
Bse3DI GCAATG 1 cut(s) 301
BseDI CCNNGG 4 cut(s) 57, 421, 603, 612
BseGI GGATG 2 cut(s) 215, 506
BseLI CCNNNNNNNGG 1 cut(s) 970
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 3 cut(s) 9, 89, 657
BseNI ACTGG 2 cut(s) 274, 529
BseRI GAGGAG 1 cut(s) 620
BseSI GKGCMC 2 cut(s) 339, 496
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 3 cut(s) 743, 865, 914
BseYI CCCAGC 1 cut(s) 969
BsgI GTGCAG 3 cut(s) 825, 864, 913
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 5 cut(s) 5, 62, 179, 538, 611
BshNI GGYRCC 1 cut(s) 425
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 2 cut(s) 180, 219
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 1 cut(s) 970
BsmAI GTCTC 3 cut(s) 138, 275, 372
BsmFI GGGAC 1 cut(s) 986
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 5 cut(s) 5, 62, 179, 538, 611
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 339, 496
Bsp143I GATC 4 cut(s) 28, 739, 853, 902
BspACI CCGC 5 cut(s) 176, 213, 429, 564, 967
BspANI GGCC 5 cut(s) 5, 62, 179, 538, 611
BspCNI CTCAG 3 cut(s) 10, 88, 658
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 427, 529
BspPI GGATC 3 cut(s) 23, 848, 897
BspT107I GGYRCC 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 2 cut(s) 274, 529
BssECI CCNNGG 4 cut(s) 57, 421, 603, 612
BssMI GATC 4 cut(s) 28, 739, 853, 902
BssT1I CCWWGG 2 cut(s) 421, 612
Bst4CI ACNGT 3 cut(s) 120, 454, 724
BstBAI YACGTR 2 cut(s) 895, 944
BstC8I GCNNGC 2 cut(s) 433, 736
BstDEI CTNAG 6 cut(s) 18, 75, 263, 369, 559, 666
BstF5I GGATG 2 cut(s) 215, 506
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 4 cut(s) 31, 742, 856, 905
BstMAI GTCTC 3 cut(s) 138, 275, 372
BstMBI GATC 4 cut(s) 28, 739, 853, 902
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 2 cut(s) 432, 954
BstNSI RCATGY 1 cut(s) 738
BstSLI GKGCMC 2 cut(s) 339, 496
BstSNI TACGTA 2 cut(s) 895, 944
BstV1I GCAGC 3 cut(s) 743, 865, 914
BstV2I GAAGAC 1 cut(s) 999
BstX2I RGATCY 2 cut(s) 853, 902
BstYI RGATCY 2 cut(s) 853, 902
BstZI CGGCCG 1 cut(s) 177
BsuRI GGCC 5 cut(s) 5, 62, 179, 538, 611
BtsCI GGATG 2 cut(s) 215, 506
BtsIMutI CAGTG 2 cut(s) 125, 281
Cac8I GCNNGC 2 cut(s) 433, 736
CciI TCATGA 1 cut(s) 283
CfoI GCGC 1 cut(s) 401
Csp6I GTAC 2 cut(s) 892, 941
CspCI CAANNNNNGTGG 1 cut(s) 986
CviAII CATG 6 cut(s) 284, 343, 353, 735, 844, 1001
CviQI GTAC 2 cut(s) 892, 941
DdeI CTNAG 6 cut(s) 18, 75, 263, 369, 559, 666
DpnI GATC 4 cut(s) 30, 741, 855, 904
DpnII GATC 4 cut(s) 28, 739, 853, 902
DrdI GACNNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 130
EaeI YGGCCR 2 cut(s) 3, 177
EagI CGGCCG 1 cut(s) 177
EclXI CGGCCG 1 cut(s) 177
Eco105I TACGTA 2 cut(s) 895, 944
Eco130I CCWWGG 2 cut(s) 421, 612
Eco31I GGTCTC 1 cut(s) 275
Eco47III AGCGCT 1 cut(s) 400
Eco52I CGGCCG 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 843, 892
EcoT14I CCWWGG 2 cut(s) 421, 612
ErhI CCWWGG 2 cut(s) 421, 612
FaeI CATG 6 cut(s) 287, 346, 356, 738, 847, 1004
FalI AAGNNNNNCTT 2 cut(s) 941, 973
FaqI GGGAC 1 cut(s) 986
FatI CATG 6 cut(s) 283, 342, 352, 734, 843, 1000
Fnu4HI GCNGC 4 cut(s) 177, 732, 879, 928
FokI GGATG 2 cut(s) 202, 513
Fsp4HI GCNGC 4 cut(s) 177, 732, 879, 928
FspBI CTAG 2 cut(s) 497, 512
GlaI GCGC 1 cut(s) 400
GluI GCNGC 4 cut(s) 177, 732, 879, 928
GsaI CCCAGC 1 cut(s) 973
GsuI CTGGAG 2 cut(s) 257, 1006
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 5 cut(s) 5, 62, 179, 538, 611
HapII CCGG 2 cut(s) 180, 219
HhaI GCGC 1 cut(s) 401
Hin1II CATG 6 cut(s) 287, 346, 356, 738, 847, 1004
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HindIII AAGCTT 1 cut(s) 955
HinfI GANTC 3 cut(s) 202, 679, 829
HpaII CCGG 2 cut(s) 180, 219
HphI GGTGA 3 cut(s) 46, 241, 773
Hpy166II GTNNAC 4 cut(s) 123, 249, 337, 701
Hpy188I TCNGA 7 cut(s) 28, 207, 229, 484, 604, 862, 911
Hpy188III TCNNGA 7 cut(s) 83, 284, 446, 512, 635, 676, 985
Hpy8I GTNNAC 4 cut(s) 123, 249, 337, 701
HpyAV CCTTC 5 cut(s) 600, 782, 786, 867, 916
HpyCH4III ACNGT 3 cut(s) 120, 454, 724
HpyCH4IV ACGT 2 cut(s) 894, 943
HpyCH4V TGCA 8 cut(s) 279, 301, 337, 543, 731, 806, 881, 930
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 954
HpyF3I CTNAG 6 cut(s) 18, 75, 263, 369, 559, 666
HpySE526I ACGT 2 cut(s) 894, 943
Hsp92II CATG 6 cut(s) 287, 346, 356, 738, 847, 1004
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 4 cut(s) 28, 739, 853, 902
Lsp1109I GCAGC 3 cut(s) 743, 865, 914
LweI GCATC 5 cut(s) 34, 224, 507, 634, 718
MaeI CTAG 2 cut(s) 497, 512
MaeII ACGT 2 cut(s) 894, 943
MaeIII GTNAC 2 cut(s) 461, 718
MalI GATC 4 cut(s) 30, 741, 855, 904
MboI GATC 4 cut(s) 28, 739, 853, 902
MboII GAAGA 2 cut(s) 320, 1004
MflI RGATCY 2 cut(s) 853, 902
MhlI GDGCHC 2 cut(s) 339, 496
MluCI AATT 3 cut(s) 142, 170, 582
MlyI GAGTC 1 cut(s) 673
MseI TTAA 1 cut(s) 9
MslI CAYNNNNRTG 2 cut(s) 499, 747
MspA1I CMGCKG 1 cut(s) 969
MspI CCGG 2 cut(s) 180, 219
Mva1269I GAATGC 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 432, 954
NdeII GATC 4 cut(s) 28, 739, 853, 902
NlaIII CATG 6 cut(s) 287, 346, 356, 738, 847, 1004
NlaIV GGNNCC 2 cut(s) 427, 529
NspI RCATGY 1 cut(s) 738
OliI CACNNNNGTG 1 cut(s) 747
PaeI GCATGC 1 cut(s) 738
PagI TCATGA 1 cut(s) 283
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 202, 829
PkrI GCNGC 4 cut(s) 178, 733, 880, 929
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 2 cut(s) 895, 944
PsiI TTATAA 1 cut(s) 129
PspFI CCCAGC 1 cut(s) 969
PspN4I GGNNCC 2 cut(s) 427, 529
PsuI RGATCY 2 cut(s) 853, 902
RsaI GTAC 2 cut(s) 893, 942
RsaNI GTAC 2 cut(s) 892, 941
RseI CAYNNNNRTG 2 cut(s) 499, 747
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 4 cut(s) 177, 732, 879, 928
Sau3AI GATC 4 cut(s) 28, 739, 853, 902
SchI GAGTC 1 cut(s) 673
SduI GDGCHC 2 cut(s) 339, 496
SfaNI GCATC 5 cut(s) 34, 224, 507, 634, 718
SmiMI CAYNNNNRTG 2 cut(s) 499, 747
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
SnaBI TACGTA 2 cut(s) 895, 944
SphI GCATGC 1 cut(s) 738
Sse9I AATT 3 cut(s) 142, 170, 582
SsiI CCGC 5 cut(s) 176, 213, 429, 564, 967
SspMI CTAG 2 cut(s) 497, 512
StyI CCWWGG 2 cut(s) 421, 612
TaaI ACNGT 3 cut(s) 120, 454, 724
TaiI ACGT 2 cut(s) 897, 946
TaqI TCGA 1 cut(s) 357
TasI AATT 3 cut(s) 142, 170, 582
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 202, 829
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 2 cut(s) 125, 281
TseI GCWGC 3 cut(s) 731, 878, 927
TspDTI ATGAA 1 cut(s) 272
TspRI CASTG 2 cut(s) 125, 281
VneI GTGCAC 1 cut(s) 335
XapI RAATTY 1 cut(s) 170
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 738
XcmI CCANNNNNNNNNTGG 2 cut(s) 486, 619
XspI CTAG 2 cut(s) 497, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.