RLG00000008579

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001298
Physical Location & Seq
Reverse (-)
32458978 .. 32462928
3951 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008579

Sequence Viewer

Length: 1191 bp
ATGGCCGTTAAGGTCGCTGAGGTTTCTGATCCAAAAAAGGTTGATGCTTTATTCACCGAGGCCAAACTTCTCTCTCAGTTTCAGGACTGCCCTTTTGTTATCAACTCTTTTGGAGAACAGTGGACTTATAAGTCTCATCATAATTCTTTCTCTTTCAATCTGCTATTAGAATTTGCGGCCGGAGGCACTATAAGAGATAGGATTCAGAAATCCGCATCCGGCTTGTCCGAATGTGAGATAAAAGAGTTCACCAAGTCAGTTCTTAGTGGTCTCCAGTGCATTCATGACAAAGGTTATGTGCATTGCGACATAAAGCCCGATAACATTCTTCTTGTGCACGACATGACTTCTCATGTCGATTTTGTGGCTAAGATAGGAGACTTTGGATTGACAAAGAGCGCTCAAAATGAGTATTCTCGCCAAGGCACCGCAAGCTATTTGCCTCCTGAAACTGTTATCAGTAACATTCAAGGGAAGCCATCAGACATTTGGGCACTAGGATGTTTAGTTCTAGAGATGTTGACTGGAACCTTGTGGCCTTGCAAGACAAGTGAGGACTTAGGCGGTTGGGTTTCAAATCCAATTATTCCTTCACACTTGTCCGAGGAGGCCAAGGATTTTTTGGCAAAGTGTCTGGATATAGATGCTTCCAAGAGATTTACGGCTGAGAGGCTCTTGACTCACTCATTTCTTATGAGTGAACAGAGCGTTGAACAAGCATTAGCTGAGAAAATCGGCAGTGTTTTTGGCTATCCCCTATCTCAGTGTCCCTTTAATTCAAAAGAGGATTCTGAAGAGCACACAGTTACTGTTGATGCCGCATGCGATCGCAAAAGTGGGAAATCTGGTGATGGAGTAGTGGTGCGAGATAAAGAAGGAAGGCTCAAGGGTGCAGTTCAACAGCCCATACCAGTTTTCACTCCATCTAATTTCATTCATAAGATTGGCTGCATTGCTCTCCAATCTGTCTTAAGACAGATATGGAAGGAGCAAATTTCGATAAGATTGGATCTCGTGATGCCAGAGATTCGTAGCTATTACATGGAGCAAGATCCTTCAGAGATTGGAAACCAAGCTGCACTCATTCAGTACGTAGCAAGGAAAGCTTTAGCCTCCGCTGGGACGAGATTTTCTGGAGGGAAGACCACCATGCTTGAAGGCATCTTAGTGGTTGATAGTAGTAGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

397

Amino Acids

43.68

Weight (kDa)

5.74

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 231 8.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 2 - 226 4.4e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 129
AasI GACNNNNNNGTC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 425
AciI CCGC 6 cut(s) 176, 213, 429, 564, 819, 1116
AclWI GGATC 3 cut(s) 23, 1017, 1046
AcoI YGGCCR 2 cut(s) 3, 177
AcsI RAATTY 2 cut(s) 170, 993
AcuI CTGAAG 2 cut(s) 813, 1041
AfaI GTAC 1 cut(s) 1091
AfeI AGCGCT 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 1119
AflII CTTAAG 1 cut(s) 970
AgsI TTSAA 7 cut(s) 157, 470, 576, 713, 780, 899, 1157
AjuI GAANNNNNNNTTGG 2 cut(s) 574, 606
AloI GAACNNNNNNTCC 2 cut(s) 492, 524
AluBI AGCT 5 cut(s) 435, 725, 1035, 1076, 1106
AluI AGCT 5 cut(s) 435, 725, 1035, 1076, 1106
Alw21I GWGCWC 2 cut(s) 339, 801
Alw26I GTCTC 3 cut(s) 138, 275, 372
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 3 cut(s) 23, 1017, 1046
AlwNI CAGNNNCTG 1 cut(s) 809
Aor51HI AGCGCT 1 cut(s) 400
AoxI GGCC 5 cut(s) 3, 60, 177, 536, 609
ApaLI GTGCAC 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 948, 1076
ApoI RAATTY 2 cut(s) 170, 993
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AsiSI GCGATCGC 1 cut(s) 829
AspLEI GCGC 1 cut(s) 401
AsuHPI GGTGA 3 cut(s) 46, 241, 860
BaeGI GKGCMC 2 cut(s) 339, 496
BanI GGYRCC 1 cut(s) 425
BauI CACGAG 1 cut(s) 1013
BbsI GAAGAC 1 cut(s) 1148
Bbv12I GWGCWC 2 cut(s) 339, 801
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 2 cut(s) 935, 1063
BccI CCATC 3 cut(s) 487, 845, 931
BceAI ACGGC 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 138, 275, 372
BfaI CTAG 3 cut(s) 497, 512, 1189
BfoI RGCGCY 1 cut(s) 402
BfrI CTTAAG 1 cut(s) 970
BisI GCNGC 4 cut(s) 177, 819, 949, 1077
BlsI GCNGC 4 cut(s) 178, 820, 950, 1078
BmiI GGNNCC 2 cut(s) 427, 529
BmsI GCATC 6 cut(s) 34, 224, 634, 805, 1008, 1170
BpiI GAAGAC 1 cut(s) 1148
BpmI CTGGAG 2 cut(s) 257, 1155
Bpu10I CCTNAGC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 869
BsaAI YACGTR 1 cut(s) 1093
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 4 cut(s) 57, 421, 603, 612
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bsc4I CCNNNNNNNGG 1 cut(s) 1119
Bse1I ACTGG 3 cut(s) 274, 529, 911
Bse3DI GCAATG 2 cut(s) 301, 951
BseDI CCNNGG 4 cut(s) 57, 421, 603, 612
BseGI GGATG 2 cut(s) 215, 506
BseLI CCNNNNNNNGG 1 cut(s) 1119
BseMI GCAATG 2 cut(s) 301, 951
BseMII CTCAG 5 cut(s) 9, 89, 657, 717, 776
BseNI ACTGG 3 cut(s) 274, 529, 911
BseRI GAGGAG 1 cut(s) 620
BseSI GKGCMC 2 cut(s) 339, 496
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 2 cut(s) 935, 1063
BseYI CCCAGC 1 cut(s) 1118
BsgI GTGCAG 2 cut(s) 912, 1062
Bsh1285I CGRYCG 2 cut(s) 180, 829
BshFI GGCC 5 cut(s) 5, 62, 179, 538, 611
BshNI GGYRCC 1 cut(s) 425
BsiEI CGRYCG 2 cut(s) 180, 829
BsiHKAI GWGCWC 2 cut(s) 339, 801
BsiSI CCGG 2 cut(s) 180, 219
BslFI GGGAC 2 cut(s) 753, 1135
BslI CCNNNNNNNGG 1 cut(s) 1119
BsmAI GTCTC 3 cut(s) 138, 275, 372
BsmFI GGGAC 2 cut(s) 753, 1135
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 5 cut(s) 5, 62, 179, 538, 611
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 3 cut(s) 339, 496, 801
Bsp143I GATC 4 cut(s) 28, 826, 1009, 1051
BspACI CCGC 6 cut(s) 176, 213, 429, 564, 819, 1116
BspANI GGCC 5 cut(s) 5, 62, 179, 538, 611
BspCNI CTCAG 5 cut(s) 10, 88, 658, 718, 775
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 427, 529
BspPI GGATC 3 cut(s) 23, 1017, 1046
BspQI GCTCTTC 1 cut(s) 789
BspT107I GGYRCC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 970
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 2 cut(s) 301, 951
BsrI ACTGG 3 cut(s) 274, 529, 911
BssECI CCNNGG 4 cut(s) 57, 421, 603, 612
BssMI GATC 4 cut(s) 28, 826, 1009, 1051
BssSI CACGAG 1 cut(s) 1013
BssT1I CCWWGG 2 cut(s) 421, 612
Bst2BI CACGAG 1 cut(s) 1013
Bst4CI ACNGT 4 cut(s) 120, 454, 805, 811
Bst6I CTCTTC 1 cut(s) 789
BstAFI CTTAAG 1 cut(s) 970
BstBAI YACGTR 1 cut(s) 1093
BstC8I GCNNGC 2 cut(s) 433, 823
BstDEI CTNAG 9 cut(s) 18, 75, 263, 369, 559, 666, 726, 762, 1165
BstF5I GGATG 2 cut(s) 215, 506
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 4 cut(s) 31, 829, 1012, 1054
BstMAI GTCTC 3 cut(s) 138, 275, 372
BstMBI GATC 4 cut(s) 28, 826, 1009, 1051
BstMCI CGRYCG 2 cut(s) 180, 829
BstMWI GCNNNNNNNGC 2 cut(s) 432, 1103
BstNSI RCATGY 1 cut(s) 825
BstSLI GKGCMC 2 cut(s) 339, 496
BstSNI TACGTA 1 cut(s) 1093
BstV1I GCAGC 2 cut(s) 935, 1063
BstV2I GAAGAC 1 cut(s) 1148
BstX2I RGATCY 2 cut(s) 1009, 1051
BstYI RGATCY 2 cut(s) 1009, 1051
BstZI CGGCCG 1 cut(s) 177
BsuRI GGCC 5 cut(s) 5, 62, 179, 538, 611
BtsCI GGATG 2 cut(s) 215, 506
BtsI GCAGTG 1 cut(s) 745
BtsIMutI CAGTG 4 cut(s) 125, 281, 745, 770
Cac8I GCNNGC 2 cut(s) 433, 823
CaiI CAGNNNCTG 1 cut(s) 809
CciI TCATGA 1 cut(s) 283
CfoI GCGC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 1090
CspCI CAANNNNNGTGG 2 cut(s) 1135, 1170
CviAII CATG 6 cut(s) 284, 343, 353, 822, 1042, 1150
CviQI GTAC 1 cut(s) 1090
DdeI CTNAG 9 cut(s) 18, 75, 263, 369, 559, 666, 726, 762, 1165
DpnI GATC 4 cut(s) 30, 828, 1011, 1053
DpnII GATC 4 cut(s) 28, 826, 1009, 1051
DrdI GACNNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 130
EaeI YGGCCR 2 cut(s) 3, 177
EagI CGGCCG 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
EclXI CGGCCG 1 cut(s) 177
Eco105I TACGTA 1 cut(s) 1093
Eco130I CCWWGG 2 cut(s) 421, 612
Eco31I GGTCTC 1 cut(s) 275
Eco47III AGCGCT 1 cut(s) 400
Eco52I CGGCCG 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 813, 1041
EcoT14I CCWWGG 2 cut(s) 421, 612
ErhI CCWWGG 2 cut(s) 421, 612
FaeI CATG 6 cut(s) 287, 346, 356, 825, 1045, 1153
FalI AAGNNNNNCTT 2 cut(s) 1090, 1122
FaqI GGGAC 2 cut(s) 753, 1135
FatI CATG 6 cut(s) 283, 342, 352, 821, 1041, 1149
Fnu4HI GCNGC 4 cut(s) 177, 819, 949, 1077
FokI GGATG 2 cut(s) 202, 513
Fsp4HI GCNGC 4 cut(s) 177, 819, 949, 1077
FspBI CTAG 3 cut(s) 497, 512, 1189
GlaI GCGC 1 cut(s) 400
GluI GCNGC 4 cut(s) 177, 819, 949, 1077
GsaI CCCAGC 1 cut(s) 1122
GsuI CTGGAG 2 cut(s) 257, 1155
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 5 cut(s) 5, 62, 179, 538, 611
HapII CCGG 2 cut(s) 180, 219
HhaI GCGC 1 cut(s) 401
Hin1II CATG 6 cut(s) 287, 346, 356, 825, 1045, 1153
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 1104
HinfI GANTC 4 cut(s) 202, 679, 788, 1027
HpaII CCGG 2 cut(s) 180, 219
HphI GGTGA 3 cut(s) 46, 241, 860
Hpy166II GTNNAC 5 cut(s) 123, 249, 337, 522, 701
Hpy188I TCNGA 7 cut(s) 28, 207, 229, 484, 604, 793, 1060
Hpy188III TCNNGA 8 cut(s) 83, 284, 446, 512, 635, 676, 1015, 1134
Hpy8I GTNNAC 5 cut(s) 123, 249, 337, 522, 701
HpyAV CCTTC 6 cut(s) 600, 869, 873, 979, 1065, 1151
HpyCH4III ACNGT 4 cut(s) 120, 454, 805, 811
HpyCH4IV ACGT 1 cut(s) 1092
HpyCH4V TGCA 7 cut(s) 279, 301, 337, 543, 893, 951, 1079
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 1103
HpyF3I CTNAG 9 cut(s) 18, 75, 263, 369, 559, 666, 726, 762, 1165
HpySE526I ACGT 1 cut(s) 1092
Hsp92II CATG 6 cut(s) 287, 346, 356, 825, 1045, 1153
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 4 cut(s) 28, 826, 1009, 1051
LguI GCTCTTC 1 cut(s) 789
LmnI GCTCC 2 cut(s) 988, 1045
Lsp1109I GCAGC 2 cut(s) 935, 1063
LweI GCATC 6 cut(s) 34, 224, 634, 805, 1008, 1170
MaeI CTAG 3 cut(s) 497, 512, 1189
MaeII ACGT 1 cut(s) 1092
MaeIII GTNAC 2 cut(s) 461, 805
MalI GATC 4 cut(s) 30, 828, 1011, 1053
MboI GATC 4 cut(s) 28, 826, 1009, 1051
MboII GAAGA 3 cut(s) 320, 806, 1153
MflI RGATCY 2 cut(s) 1009, 1051
MhlI GDGCHC 3 cut(s) 339, 496, 801
MluCI AATT 6 cut(s) 142, 170, 582, 775, 928, 993
MlyI GAGTC 1 cut(s) 673
MseI TTAA 3 cut(s) 9, 774, 971
MslI CAYNNNNRTG 2 cut(s) 499, 1166
MspA1I CMGCKG 1 cut(s) 1118
MspCI CTTAAG 1 cut(s) 970
MspI CCGG 2 cut(s) 180, 219
Mva1269I GAATGC 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 432, 1103
NdeII GATC 4 cut(s) 28, 826, 1009, 1051
NlaIII CATG 6 cut(s) 287, 346, 356, 825, 1045, 1153
NlaIV GGNNCC 2 cut(s) 427, 529
NspI RCATGY 1 cut(s) 825
PaeI GCATGC 1 cut(s) 825
PagI TCATGA 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 789
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 202, 788, 1027
PkrI GCNGC 4 cut(s) 178, 820, 950, 1078
Ple19I CGATCG 1 cut(s) 829
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 1093
PsiI TTATAA 1 cut(s) 129
PspFI CCCAGC 1 cut(s) 1118
PspN4I GGNNCC 2 cut(s) 427, 529
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 2 cut(s) 1009, 1051
PvuI CGATCG 1 cut(s) 829
RgaI GCGATCGC 1 cut(s) 829
RsaI GTAC 1 cut(s) 1091
RsaNI GTAC 1 cut(s) 1090
RseI CAYNNNNRTG 2 cut(s) 499, 1166
SapI GCTCTTC 1 cut(s) 789
SaqAI TTAA 3 cut(s) 9, 774, 971
SatI GCNGC 4 cut(s) 177, 819, 949, 1077
Sau3AI GATC 4 cut(s) 28, 826, 1009, 1051
SchI GAGTC 1 cut(s) 673
SduI GDGCHC 3 cut(s) 339, 496, 801
SfaAI GCGATCGC 1 cut(s) 829
SfaNI GCATC 6 cut(s) 34, 224, 634, 805, 1008, 1170
SgfI GCGATCGC 1 cut(s) 829
SmiMI CAYNNNNRTG 2 cut(s) 499, 1166
SmlI CTYRAG 2 cut(s) 884, 970
SmoI CTYRAG 2 cut(s) 884, 970
SnaBI TACGTA 1 cut(s) 1093
SphI GCATGC 1 cut(s) 825
Sse9I AATT 6 cut(s) 142, 170, 582, 775, 928, 993
SsiI CCGC 6 cut(s) 176, 213, 429, 564, 819, 1116
SspMI CTAG 3 cut(s) 497, 512, 1189
StyI CCWWGG 2 cut(s) 421, 612
TaaI ACNGT 4 cut(s) 120, 454, 805, 811
TaiI ACGT 1 cut(s) 1095
TaqI TCGA 2 cut(s) 357, 998
TasI AATT 6 cut(s) 142, 170, 582, 775, 928, 993
TauI GCSGC 2 cut(s) 179, 821
TfiI GAWTC 3 cut(s) 202, 788, 1027
Tru1I TTAA 3 cut(s) 9, 774, 971
Tru9I TTAA 3 cut(s) 9, 774, 971
TscAI CASTG 4 cut(s) 125, 281, 745, 770
TseI GCWGC 2 cut(s) 948, 1076
TspDTI ATGAA 3 cut(s) 272, 922, 926
TspRI CASTG 4 cut(s) 125, 281, 745, 770
Vha464I CTTAAG 1 cut(s) 970
VneI GTGCAC 1 cut(s) 335
XapI RAATTY 2 cut(s) 170, 993
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 825
XcmI CCANNNNNNNNNTGG 2 cut(s) 486, 619
XspI CTAG 3 cut(s) 497, 512, 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.