RchiOBHm_Chr2g0149301

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001298
Physical Location & Seq
Forward (+)
67175416 .. 67176607
1192 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51871

Sequence Viewer

Length: 1095 bp
ATGAGTGAATCAGACTATTTTAAGGCCATGGAAAAGCTCGAGAAGAAGAGGAAAAGAATTCAGGAGGAAGAGCCGGATTCTGGCAACGTCGGTCGGACCAAACGGTGGGTTCGACAAAGGTTCCTCGGCGCAGGACAATTCGGTACGGTTTTCTTGGCTAGTCCGGCCAAACCAGGTTCCAAACTGCCGCCGGTTATGGCCGTGAAGTCGGCGATGCTCTCGGAATCTGCCGACTTGGTTAAAGAAAGTCGACTTCTCAAGAAATTCAAGAACTGCCCTTTTATCCTTGACACTTACGGCAGCGATGTGACTAAAGATGTTGAAGGTGAGGAGTGGTTCAACGTTTTCTTGGAGTATGCTCAAAGAGGAACCATAGAGGATTGGATTCAGAAACTCAAGAATTTCGGGTTGCATGAGCTGCACGTAAGGAGGTATACACAGTCACTATTGAAGGGGCTCAAGTACATTCATGGAAAGGGTTTTGTGCACTGTGACATTAAGCCTGCCAATGTATTGCTAGTGCGTGACGGTCCTGATGATACCGGGTTTGGATCTTACGTGGCGAAAATTGGGGATTTAGGGCTGGCAAAGAGGGCTGACAAGTACAATGTGATGAAGCGTTCTGATTATATAAATGGCACTGAGTTGTATATGTCTCCAGAAGTATCGCTTCACAACATTCAGGAGCCGCCAGCCGACATCTGGGCATTAGGCTGCACTGTTCTGCAGATGTTAACCGGGATGGAGTTTTGGGATGTGAACACTGACTTAAGAGATGACTGGGGGGTTCCGATTGAGATTCCGATTATCCCTGAAAGGCTGAGCAACGAGGCTAAGGATTTCTTGGGGAAGTGCTTCATATTGGACCCTTTGGAGAGATACACGGCTGAGATGCTTTTGAAGCACCCTTTTATGTGCAAAAGCTTGCGAATATGGACAGTCTCATCTCGTGAATTGGTTACAAGAAGCCGAGTTTGCCAAGGACCAGCTGCAATTCCTCTAATGGGTGCGAGAGCTGTACCTAGTGCCAGTTTTGATCTCTTTTATCTTTCTCTTGGTTGTTTTAAGGTTGGTGTTGTTCTTTCACTGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

364

Amino Acids

41.19

Weight (kDa)

8.52

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 38 - 305 7.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 40 - 299 5.4e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 2 cut(s) 250, 434
AciI CCGC 2 cut(s) 188, 689
AclI AACGTT 1 cut(s) 342
AclWI GGATC 1 cut(s) 559
AcoI YGGCCR 2 cut(s) 165, 198
AcsI RAATTY 3 cut(s) 57, 263, 400
AfaI GTAC 4 cut(s) 145, 464, 605, 1020
AfiI CCNNNNNNNGG 4 cut(s) 80, 105, 702, 1004
AflII CTTAAG 1 cut(s) 769
AgsI TTSAA 5 cut(s) 268, 323, 340, 451, 901
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 5 cut(s) 37, 418, 924, 989, 1016
AluI AGCT 5 cut(s) 37, 418, 924, 989, 1016
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 2 cut(s) 660, 946
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 1 cut(s) 559
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 3 cut(s) 24, 165, 198
ApaLI GTGCAC 1 cut(s) 485
ApeKI GCWGC 4 cut(s) 300, 418, 714, 989
ApoI RAATTY 3 cut(s) 57, 263, 400
Asp700I GAANNNNTTC 1 cut(s) 854
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 4 cut(s) 96, 530, 865, 983
AsuC2I CCSGG 2 cut(s) 544, 739
AsuHPI GGTGA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 4 cut(s) 96, 530, 865, 983
BaeGI GKGCMC 1 cut(s) 489
BanII GRGCYC 1 cut(s) 459
BauI CACGAG 1 cut(s) 948
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 4 cut(s) 312, 405, 701, 976
BccI CCATC 1 cut(s) 736
BceAI ACGGC 3 cut(s) 185, 313, 900
BciT130I CCWGG 1 cut(s) 174
BcnI CCSGG 2 cut(s) 544, 739
BcoDI GTCTC 2 cut(s) 660, 946
BfaI CTAG 3 cut(s) 159, 518, 1023
BfmI CTRYAG 1 cut(s) 725
BfrI CTTAAG 1 cut(s) 769
BisI GCNGC 6 cut(s) 188, 301, 419, 689, 715, 990
BlpI GCTNAGC 1 cut(s) 821
BlsI GCNGC 6 cut(s) 189, 302, 420, 690, 716, 991
Bme1390I CCNGG 3 cut(s) 174, 544, 739
Bme18I GGWCC 4 cut(s) 96, 530, 865, 983
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 4 cut(s) 96, 530, 865, 983
BmiI GGNNCC 6 cut(s) 122, 178, 370, 687, 789, 867
BmrFI CCNGG 3 cut(s) 174, 544, 739
BmrI ACTGGG 1 cut(s) 790
BmsI GCATC 2 cut(s) 204, 882
BmuI ACTGGG 1 cut(s) 790
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 834
Bpu1102I GCTNAGC 1 cut(s) 821
BpuEI CTTGAG 3 cut(s) 242, 380, 443
BpuMI CCSGG 2 cut(s) 544, 739
BsaAI YACGTR 2 cut(s) 424, 559
BsaJI CCNNGG 3 cut(s) 27, 124, 979
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 105, 702, 1004
Bse118I RCCGGY 1 cut(s) 190
Bse1I ACTGG 3 cut(s) 785, 1029, 1092
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 27, 124, 979
BseGI GGATG 2 cut(s) 747, 760
BseLI CCNNNNNNNGG 4 cut(s) 80, 105, 702, 1004
BseMII CTCAG 3 cut(s) 633, 812, 879
BseNI ACTGG 3 cut(s) 785, 1029, 1092
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 489
BseXI GCAGC 4 cut(s) 312, 405, 701, 976
BsgI GTGCAG 2 cut(s) 404, 700
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 3 cut(s) 26, 167, 200
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 5 cut(s) 74, 164, 191, 543, 738
BslI CCNNNNNNNGG 4 cut(s) 80, 105, 702, 1004
BsmAI GTCTC 2 cut(s) 660, 946
BsnI GGCC 3 cut(s) 26, 167, 200
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 459, 489
Bsp143I GATC 2 cut(s) 551, 1036
Bsp1720I GCTNAGC 1 cut(s) 821
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 2 cut(s) 188, 689
BspANI GGCC 3 cut(s) 26, 167, 200
BspCNI CTCAG 3 cut(s) 634, 813, 880
BspLI GGNNCC 6 cut(s) 122, 178, 370, 687, 789, 867
BspMAI CTGCAG 1 cut(s) 729
BspPI GGATC 1 cut(s) 559
BspQI GCTCTTC 1 cut(s) 63
BspTI CTTAAG 1 cut(s) 769
BsrFI RCCGGY 1 cut(s) 190
BsrI ACTGG 3 cut(s) 785, 1029, 1092
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 3 cut(s) 27, 124, 979
BssMI GATC 2 cut(s) 551, 1036
BssNAI GTATAC 1 cut(s) 435
BssSI CACGAG 1 cut(s) 948
BssT1I CCWWGG 2 cut(s) 27, 979
Bst1107I GTATAC 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 948
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 7 cut(s) 105, 148, 441, 491, 530, 721, 940
Bst6I CTCTTC 2 cut(s) 41, 63
BstAFI CTTAAG 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 418
BstBAI YACGTR 2 cut(s) 424, 559
BstC8I GCNNGC 4 cut(s) 504, 585, 693, 926
BstDEI CTNAG 4 cut(s) 642, 821, 834, 888
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 747, 760
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 554, 1039
BstMAI GTCTC 2 cut(s) 660, 946
BstMBI GATC 2 cut(s) 551, 1036
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 5 cut(s) 164, 418, 593, 901, 975
BstNI CCWGG 1 cut(s) 174
BstSCI CCNGG 3 cut(s) 172, 542, 737
BstSFI CTRYAG 1 cut(s) 725
BstSLI GKGCMC 1 cut(s) 489
BstV1I GCAGC 4 cut(s) 312, 405, 701, 976
BstX2I RGATCY 1 cut(s) 551
BstYI RGATCY 1 cut(s) 551
BstZ17I GTATAC 1 cut(s) 435
BsuRI GGCC 3 cut(s) 26, 167, 200
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 2 cut(s) 227, 318
BtsCI GGATG 2 cut(s) 747, 760
BtsIMutI CAGTG 5 cut(s) 487, 639, 717, 762, 1085
Cac8I GCNNGC 4 cut(s) 504, 585, 693, 926
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 4 cut(s) 96, 530, 865, 983
CsiI ACCWGGT 1 cut(s) 172
Csp6I GTAC 4 cut(s) 144, 463, 604, 1019
CviAII CATG 3 cut(s) 28, 413, 470
CviQI GTAC 4 cut(s) 144, 463, 604, 1019
DdeI CTNAG 4 cut(s) 642, 821, 834, 888
DpnI GATC 2 cut(s) 553, 1038
DpnII GATC 2 cut(s) 551, 1036
EaeI YGGCCR 2 cut(s) 165, 198
Eam1104I CTCTTC 2 cut(s) 41, 63
EarI CTCTTC 2 cut(s) 41, 63
Eco130I CCWWGG 2 cut(s) 27, 979
Eco24I GRGCYC 1 cut(s) 459
Eco47I GGWCC 4 cut(s) 96, 530, 865, 983
Eco88I CYCGRG 1 cut(s) 38
EcoRI GAATTC 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 172
EcoT14I CCWWGG 2 cut(s) 27, 979
EcoT38I GRGCYC 1 cut(s) 459
ErhI CCWWGG 2 cut(s) 27, 979
FaeI CATG 3 cut(s) 31, 416, 473
FalI AAGNNNNNCTT 4 cut(s) 654, 686, 827, 859
FatI CATG 3 cut(s) 27, 412, 469
FblI GTMKAC 2 cut(s) 250, 434
Fnu4HI GCNGC 6 cut(s) 188, 301, 419, 689, 715, 990
FokI GGATG 2 cut(s) 754, 767
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 6 cut(s) 188, 301, 419, 689, 715, 990
FspBI CTAG 3 cut(s) 159, 518, 1023
GlaI GCGC 1 cut(s) 130
GluI GCNGC 6 cut(s) 188, 301, 419, 689, 715, 990
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 3 cut(s) 26, 167, 200
HapII CCGG 5 cut(s) 74, 164, 191, 543, 738
HhaI GCGC 1 cut(s) 131
Hin1II CATG 3 cut(s) 31, 416, 473
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 2 cut(s) 251, 735
HindII GTYRAC 2 cut(s) 251, 735
HindIII AAGCTT 1 cut(s) 922
HinfI GANTC 5 cut(s) 8, 77, 224, 385, 799
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 5 cut(s) 74, 164, 191, 543, 738
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 5 cut(s) 251, 435, 487, 735, 760
Hpy188I TCNGA 7 cut(s) 13, 96, 223, 390, 625, 792, 804
Hpy188III TCNNGA 9 cut(s) 40, 62, 259, 268, 397, 533, 659, 683, 950
Hpy8I GTNNAC 5 cut(s) 251, 435, 487, 735, 760
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 2 cut(s) 317, 445
HpyCH4III ACNGT 7 cut(s) 105, 148, 441, 491, 530, 721, 940
HpyCH4IV ACGT 4 cut(s) 87, 342, 423, 558
HpyCH4V TGCA 7 cut(s) 412, 421, 487, 717, 727, 918, 992
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 418, 593, 901, 975
HpyF3I CTNAG 4 cut(s) 642, 821, 834, 888
HpySE526I ACGT 4 cut(s) 87, 342, 423, 558
Hsp92II CATG 3 cut(s) 31, 416, 473
HspAI GCGC 1 cut(s) 129
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 2 cut(s) 551, 1036
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 4 cut(s) 312, 405, 701, 976
LweI GCATC 2 cut(s) 204, 882
MabI ACCWGGT 1 cut(s) 172
MaeI CTAG 3 cut(s) 159, 518, 1023
MaeII ACGT 4 cut(s) 87, 342, 423, 558
MaeIII GTNAC 5 cut(s) 307, 441, 491, 524, 958
MalI GATC 2 cut(s) 553, 1038
MboI GATC 2 cut(s) 551, 1036
MboII GAAGA 3 cut(s) 55, 58, 80
MflI RGATCY 1 cut(s) 551
MhlI GDGCHC 2 cut(s) 459, 489
MluCI AATT 7 cut(s) 57, 137, 263, 400, 567, 953, 993
MmeI TCCRAC 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 854
MseI TTAA 6 cut(s) 21, 240, 498, 734, 770, 1065
MspA1I CMGCKG 1 cut(s) 989
MspCI CTTAAG 1 cut(s) 769
MspI CCGG 5 cut(s) 74, 164, 191, 543, 738
MspR9I CCNGG 3 cut(s) 174, 544, 739
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 5 cut(s) 164, 418, 593, 901, 975
NciI CCSGG 2 cut(s) 544, 739
NcoI CCATGG 1 cut(s) 27
NdeII GATC 2 cut(s) 551, 1036
NlaIII CATG 3 cut(s) 31, 416, 473
NlaIV GGNNCC 6 cut(s) 122, 178, 370, 687, 789, 867
NmeAIII GCCGAG 2 cut(s) 105, 995
NmuCI GTSAC 4 cut(s) 307, 441, 491, 524
PaeR7I CTCGAG 1 cut(s) 38
PciSI GCTCTTC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 854
PfeI GAWTC 5 cut(s) 8, 77, 224, 385, 799
PflMI CCANNNNNTGG 1 cut(s) 105
PkrI GCNGC 6 cut(s) 189, 302, 420, 690, 716, 991
Ppu21I YACGTR 2 cut(s) 424, 559
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 172
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 6 cut(s) 122, 178, 370, 687, 789, 867
PspPI GGNCC 4 cut(s) 96, 530, 865, 983
PstI CTGCAG 1 cut(s) 729
PsuI RGATCY 1 cut(s) 551
PvuII CAGCTG 1 cut(s) 989
RsaI GTAC 4 cut(s) 145, 464, 605, 1020
RsaNI GTAC 4 cut(s) 144, 463, 604, 1019
SalI GTCGAC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 6 cut(s) 21, 240, 498, 734, 770, 1065
SatI GCNGC 6 cut(s) 188, 301, 419, 689, 715, 990
Sau3AI GATC 2 cut(s) 551, 1036
Sau96I GGNCC 4 cut(s) 96, 530, 865, 983
ScrFI CCNGG 3 cut(s) 174, 544, 739
SduI GDGCHC 2 cut(s) 459, 489
SexAI ACCWGGT 1 cut(s) 172
SfaNI GCATC 2 cut(s) 204, 882
SfcI CTRYAG 1 cut(s) 725
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 4 cut(s) 96, 530, 865, 983
SlaI CTCGAG 1 cut(s) 38
SmlI CTYRAG 5 cut(s) 38, 257, 395, 458, 769
SmoI CTYRAG 5 cut(s) 38, 257, 395, 458, 769
Sse9I AATT 7 cut(s) 57, 137, 263, 400, 567, 953, 993
SsiI CCGC 2 cut(s) 188, 689
SspMI CTAG 3 cut(s) 159, 518, 1023
StyD4I CCNGG 3 cut(s) 172, 542, 737
StyI CCWWGG 2 cut(s) 27, 979
TaaI ACNGT 7 cut(s) 105, 148, 441, 491, 530, 721, 940
TaiI ACGT 4 cut(s) 90, 345, 426, 561
TaqI TCGA 3 cut(s) 39, 112, 250
TaqII GACCGA 1 cut(s) 80
TasI AATT 7 cut(s) 57, 137, 263, 400, 567, 953, 993
TatI WGTACW 2 cut(s) 462, 603
TauI GCSGC 2 cut(s) 190, 691
TfiI GAWTC 5 cut(s) 8, 77, 224, 385, 799
Tru1I TTAA 6 cut(s) 21, 240, 498, 734, 770, 1065
Tru9I TTAA 6 cut(s) 21, 240, 498, 734, 770, 1065
TscAI CASTG 5 cut(s) 494, 646, 724, 769, 1092
TseFI GTSAC 4 cut(s) 307, 441, 491, 524
TseI GCWGC 4 cut(s) 300, 418, 714, 989
Tsp45I GTSAC 4 cut(s) 307, 441, 491, 524
TspDTI ATGAA 3 cut(s) 458, 629, 847
TspRI CASTG 5 cut(s) 494, 646, 724, 769, 1092
Van91I CCANNNNNTGG 1 cut(s) 105
Vha464I CTTAAG 1 cut(s) 769
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 4 cut(s) 96, 530, 865, 983
XapI RAATTY 3 cut(s) 57, 263, 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 699
XhoI CTCGAG 1 cut(s) 38
XmiI GTMKAC 2 cut(s) 250, 434
XmnI GAANNNNTTC 1 cut(s) 854
XspI CTAG 3 cut(s) 159, 518, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.