RLG00000020391

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001298
Physical Location & Seq
Forward (+)
64531912 .. 64532901
990 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020391

Sequence Viewer

Length: 990 bp
ATGGAAAAGCTCGAGAAGAAGAGGAAAAGAATCCAGGAGGAAGAGCCGGATTCTGGCAACGTCGGTCGGACCAAACGGTGGGTTCGAGAAAGGTTCCTCGGCGCAGGACAATTCGGTACTGTTTTCTTGGCTAGTCCGGCCAAACCAGGTTCCAATCTGCCGCCGGTTATGGCCGTGAAGTCGGCTATGCTCTCGGAATCTGCCGACTTGGTTAAAGAAAGCCGACTTCTCAAGAAATTCAAGAACTGCCCTTTTATCCTTGACACTTACGGCAGCGATGTGACTAAAGATGTTGAAGGTGAGGAGTGGTTCAACGTTTGCTTGGAGTATGCTCAAAGAGGAACCATAGAGGATTGGATTCAGAAACTCAAGAATTTCGGGTTGCATGAGCTGCACGTAAGGAGGTATACACAGTCAGTATTGAAGGGGCTCAAGTACATTCATGGAAAGGGTTTTGTGCACTGTGACATTAAGCCTGCCAATGTATTGCTAGTGCGGGACGGTCCTGATGATACCGGGTTTGGATCTTATGTGGCGAAAATCGGGGATTTAGGGCTAGCAAAGAGGGCTGACAAGTACAATGTGATGAAGCGTTCTGATTATATAAATGGCACTGAGTTGTATATGTCTCCAGAAGTATCGCTTCACAACATTCAGGAGCCGCCAGCCGACATCTGGGCATTAGGCTGCACTGTTCTGCAGATGTTAACCGGGATGGAGTTTTGGGATGTGAACGCTGACTTAAGAGATGACTGGGGGGTTCCGATTGAGGTTCCGATTATCCCTGAAAGGCTAAGCAACGAGGCTAAGGATTTCTTGGGGAAGTGCTTCATATTGGACCCTTTGAAGAGATACACGGCTGAGATGCTTTTGAAGCACCCTTTTGTGTGCAAAAGCTTGCGAATATGGACAGTCTCATCTGTTGAATTGGTTCCAAGAAGCCGAGTTTGCCAAGGACCAGCTGCAATTCCTCTAATGGGTGCCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

330

Amino Acids

37.12

Weight (kDa)

8.35

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 28 - 296 1.5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 31 - 290 1.7e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 980
AccB7I CCANNNNNTGG 1 cut(s) 78
AccI GTMKAC 1 cut(s) 407
AciI CCGC 4 cut(s) 161, 496, 662, 984
AclI AACGTT 1 cut(s) 315
AclWI GGATC 1 cut(s) 532
AcoI YGGCCR 2 cut(s) 138, 171
AcsI RAATTY 2 cut(s) 236, 373
AfaI GTAC 3 cut(s) 118, 437, 578
AfiI CCNNNNNNNGG 4 cut(s) 53, 78, 675, 977
AflII CTTAAG 1 cut(s) 742
AgsI TTSAA 7 cut(s) 241, 296, 313, 424, 847, 874, 926
AjnI CCWGG 2 cut(s) 33, 145
AluBI AGCT 4 cut(s) 10, 391, 897, 962
AluI AGCT 4 cut(s) 10, 391, 897, 962
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 2 cut(s) 633, 919
Alw44I GTGCAC 1 cut(s) 458
AlwI GGATC 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 2 cut(s) 138, 171
ApaLI GTGCAC 1 cut(s) 458
ApeKI GCWGC 4 cut(s) 273, 391, 687, 962
ApoI RAATTY 2 cut(s) 236, 373
Asp700I GAANNNNTTC 2 cut(s) 827, 930
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 4 cut(s) 69, 503, 838, 956
AsuC2I CCSGG 2 cut(s) 517, 712
AsuHPI GGTGA 1 cut(s) 311
AsuNHI GCTAGC 1 cut(s) 556
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 4 cut(s) 69, 503, 838, 956
BaeGI GKGCMC 1 cut(s) 462
BanI GGYRCC 1 cut(s) 980
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 4 cut(s) 285, 378, 674, 949
BccI CCATC 1 cut(s) 709
BceAI ACGGC 3 cut(s) 158, 286, 873
BciT130I CCWGG 2 cut(s) 35, 147
BcnI CCSGG 2 cut(s) 517, 712
BcoDI GTCTC 2 cut(s) 633, 919
BfaI CTAG 3 cut(s) 132, 491, 557
BfmI CTRYAG 1 cut(s) 698
BfrI CTTAAG 1 cut(s) 742
BisI GCNGC 7 cut(s) 161, 274, 392, 662, 688, 963, 984
BlpI GCTNAGC 1 cut(s) 794
BlsI GCNGC 7 cut(s) 162, 275, 393, 663, 689, 964, 985
Bme1390I CCNGG 4 cut(s) 35, 147, 517, 712
Bme18I GGWCC 4 cut(s) 69, 503, 838, 956
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 4 cut(s) 69, 503, 838, 956
BmiI GGNNCC 9 cut(s) 95, 151, 343, 660, 762, 774, 840, 933, 982
BmrFI CCNGG 4 cut(s) 35, 147, 517, 712
BmrI ACTGGG 1 cut(s) 763
BmsI GCATC 1 cut(s) 855
BmtI GCTAGC 1 cut(s) 560
BmuI ACTGGG 1 cut(s) 763
BpmI CTGGAG 1 cut(s) 615
Bpu10I CCTNAGC 1 cut(s) 807
Bpu1102I GCTNAGC 1 cut(s) 794
BpuEI CTTGAG 3 cut(s) 215, 353, 416
BpuMI CCSGG 2 cut(s) 517, 712
BsaAI YACGTR 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 97, 952
Bsc4I CCNNNNNNNGG 4 cut(s) 53, 78, 675, 977
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 1 cut(s) 758
BseBI CCWGG 2 cut(s) 35, 147
BseDI CCNNGG 2 cut(s) 97, 952
BseGI GGATG 2 cut(s) 720, 733
BseLI CCNNNNNNNGG 4 cut(s) 53, 78, 675, 977
BseMII CTCAG 2 cut(s) 606, 852
BseNI ACTGG 1 cut(s) 758
BseRI GAGGAG 1 cut(s) 317
BseSI GKGCMC 1 cut(s) 462
BseXI GCAGC 4 cut(s) 285, 378, 674, 949
BsgI GTGCAG 2 cut(s) 377, 673
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 2 cut(s) 140, 173
BshNI GGYRCC 1 cut(s) 980
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 5 cut(s) 47, 137, 164, 516, 711
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 4 cut(s) 53, 78, 675, 977
BsmAI GTCTC 2 cut(s) 633, 919
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 2 cut(s) 140, 173
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 432, 462
Bsp143I GATC 1 cut(s) 524
Bsp1720I GCTNAGC 1 cut(s) 794
BspACI CCGC 4 cut(s) 161, 496, 662, 984
BspANI GGCC 2 cut(s) 140, 173
BspCNI CTCAG 2 cut(s) 607, 853
BspLI GGNNCC 9 cut(s) 95, 151, 343, 660, 762, 774, 840, 933, 982
BspMAI CTGCAG 1 cut(s) 702
BspOI GCTAGC 1 cut(s) 560
BspPI GGATC 1 cut(s) 532
BspQI GCTCTTC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 980
BspTI CTTAAG 1 cut(s) 742
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 1 cut(s) 758
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 2 cut(s) 97, 952
BssMI GATC 1 cut(s) 524
BssNAI GTATAC 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 952
Bst1107I GTATAC 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 35, 147
Bst4CI ACNGT 7 cut(s) 78, 121, 414, 464, 503, 694, 913
Bst6I CTCTTC 3 cut(s) 14, 36, 842
BstAFI CTTAAG 1 cut(s) 742
BstAPI GCANNNNNTGC 1 cut(s) 391
BstBAI YACGTR 1 cut(s) 397
BstC8I GCNNGC 4 cut(s) 477, 558, 666, 899
BstDEI CTNAG 4 cut(s) 615, 794, 807, 861
BstF5I GGATG 2 cut(s) 720, 733
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 1 cut(s) 527
BstMAI GTCTC 2 cut(s) 633, 919
BstMBI GATC 1 cut(s) 524
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 5 cut(s) 137, 391, 566, 874, 948
BstNI CCWGG 2 cut(s) 35, 147
BstSCI CCNGG 4 cut(s) 33, 145, 515, 710
BstSFI CTRYAG 1 cut(s) 698
BstSLI GKGCMC 1 cut(s) 462
BstV1I GCAGC 4 cut(s) 285, 378, 674, 949
BstX2I RGATCY 1 cut(s) 524
BstYI RGATCY 1 cut(s) 524
BstZ17I GTATAC 1 cut(s) 408
BsuRI GGCC 2 cut(s) 140, 173
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 720, 733
BtsIMutI CAGTG 3 cut(s) 460, 612, 690
Cac8I GCNNGC 4 cut(s) 477, 558, 666, 899
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 163
Cfr13I GGNCC 4 cut(s) 69, 503, 838, 956
CsiI ACCWGGT 1 cut(s) 145
Csp6I GTAC 3 cut(s) 117, 436, 577
CviAII CATG 2 cut(s) 386, 443
CviQI GTAC 3 cut(s) 117, 436, 577
DdeI CTNAG 4 cut(s) 615, 794, 807, 861
DpnI GATC 1 cut(s) 526
DpnII GATC 1 cut(s) 524
EaeI YGGCCR 2 cut(s) 138, 171
Eam1104I CTCTTC 3 cut(s) 14, 36, 842
EarI CTCTTC 3 cut(s) 14, 36, 842
Eco130I CCWWGG 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 432
Eco47I GGWCC 4 cut(s) 69, 503, 838, 956
Eco88I CYCGRG 1 cut(s) 11
EcoRII CCWGG 2 cut(s) 33, 145
EcoT14I CCWWGG 1 cut(s) 952
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 952
FaeI CATG 2 cut(s) 389, 446
FalI AAGNNNNNCTT 4 cut(s) 627, 659, 800, 832
FaqI GGGAC 1 cut(s) 512
FatI CATG 2 cut(s) 385, 442
FauI CCCGC 1 cut(s) 489
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 7 cut(s) 161, 274, 392, 662, 688, 963, 984
FokI GGATG 2 cut(s) 727, 740
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 7 cut(s) 161, 274, 392, 662, 688, 963, 984
FspBI CTAG 3 cut(s) 132, 491, 557
GlaI GCGC 1 cut(s) 103
GluI GCNGC 7 cut(s) 161, 274, 392, 662, 688, 963, 984
GsuI CTGGAG 1 cut(s) 615
HaeIII GGCC 2 cut(s) 140, 173
HapII CCGG 5 cut(s) 47, 137, 164, 516, 711
HhaI GCGC 1 cut(s) 104
Hin1II CATG 2 cut(s) 389, 446
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HindIII AAGCTT 1 cut(s) 895
HinfI GANTC 4 cut(s) 30, 50, 197, 358
HpaI GTTAAC 1 cut(s) 708
HpaII CCGG 5 cut(s) 47, 137, 164, 516, 711
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 4 cut(s) 408, 460, 708, 733
Hpy188I TCNGA 6 cut(s) 69, 196, 363, 598, 765, 777
Hpy188III TCNNGA 8 cut(s) 13, 86, 232, 241, 370, 506, 632, 656
Hpy8I GTNNAC 4 cut(s) 408, 460, 708, 733
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 2 cut(s) 290, 418
HpyCH4III ACNGT 7 cut(s) 78, 121, 414, 464, 503, 694, 913
HpyCH4IV ACGT 3 cut(s) 60, 315, 396
HpyCH4V TGCA 7 cut(s) 385, 394, 460, 690, 700, 891, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 391, 566, 874, 948
HpyF3I CTNAG 4 cut(s) 615, 794, 807, 861
HpySE526I ACGT 3 cut(s) 60, 315, 396
Hsp92II CATG 2 cut(s) 389, 446
HspAI GCGC 1 cut(s) 102
KspAI GTTAAC 1 cut(s) 708
Kzo9I GATC 1 cut(s) 524
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 1 cut(s) 658
Lsp1109I GCAGC 4 cut(s) 285, 378, 674, 949
LweI GCATC 1 cut(s) 855
MabI ACCWGGT 1 cut(s) 145
MaeI CTAG 3 cut(s) 132, 491, 557
MaeII ACGT 3 cut(s) 60, 315, 396
MaeIII GTNAC 2 cut(s) 280, 464
MalI GATC 1 cut(s) 526
MboI GATC 1 cut(s) 524
MboII GAAGA 4 cut(s) 28, 31, 53, 859
MflI RGATCY 1 cut(s) 524
MhlI GDGCHC 2 cut(s) 432, 462
MluCI AATT 5 cut(s) 110, 236, 373, 926, 966
MmeI TCCRAC 1 cut(s) 47
MroXI GAANNNNTTC 2 cut(s) 827, 930
MseI TTAA 4 cut(s) 213, 471, 707, 743
MspA1I CMGCKG 1 cut(s) 962
MspCI CTTAAG 1 cut(s) 742
MspI CCGG 5 cut(s) 47, 137, 164, 516, 711
MspR9I CCNGG 4 cut(s) 35, 147, 517, 712
MvaI CCWGG 2 cut(s) 35, 147
MwoI GCNNNNNNNGC 5 cut(s) 137, 391, 566, 874, 948
NciI CCSGG 2 cut(s) 517, 712
NdeII GATC 1 cut(s) 524
NheI GCTAGC 1 cut(s) 556
NlaIII CATG 2 cut(s) 389, 446
NlaIV GGNNCC 9 cut(s) 95, 151, 343, 660, 762, 774, 840, 933, 982
NmeAIII GCCGAG 2 cut(s) 78, 968
NmuCI GTSAC 2 cut(s) 280, 464
PaeR7I CTCGAG 1 cut(s) 11
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 82
PdmI GAANNNNTTC 2 cut(s) 827, 930
PfeI GAWTC 4 cut(s) 30, 50, 197, 358
PflMI CCANNNNNTGG 1 cut(s) 78
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 7 cut(s) 162, 275, 393, 663, 689, 964, 985
Ppu21I YACGTR 1 cut(s) 397
Psp1406I AACGTT 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 33, 145
PspGI CCWGG 2 cut(s) 33, 145
PspN4I GGNNCC 9 cut(s) 95, 151, 343, 660, 762, 774, 840, 933, 982
PspPI GGNCC 4 cut(s) 69, 503, 838, 956
PstI CTGCAG 1 cut(s) 702
PsuI RGATCY 1 cut(s) 524
PvuII CAGCTG 1 cut(s) 962
RsaI GTAC 3 cut(s) 118, 437, 578
RsaNI GTAC 3 cut(s) 117, 436, 577
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 4 cut(s) 213, 471, 707, 743
SatI GCNGC 7 cut(s) 161, 274, 392, 662, 688, 963, 984
Sau3AI GATC 1 cut(s) 524
Sau96I GGNCC 4 cut(s) 69, 503, 838, 956
ScrFI CCNGG 4 cut(s) 35, 147, 517, 712
SduI GDGCHC 2 cut(s) 432, 462
SexAI ACCWGGT 1 cut(s) 145
SfaNI GCATC 1 cut(s) 855
SfcI CTRYAG 1 cut(s) 698
Sfr274I CTCGAG 1 cut(s) 11
SinI GGWCC 4 cut(s) 69, 503, 838, 956
SlaI CTCGAG 1 cut(s) 11
SmlI CTYRAG 5 cut(s) 11, 230, 368, 431, 742
SmoI CTYRAG 5 cut(s) 11, 230, 368, 431, 742
Sse9I AATT 5 cut(s) 110, 236, 373, 926, 966
SsiI CCGC 4 cut(s) 161, 496, 662, 984
SspMI CTAG 3 cut(s) 132, 491, 557
StyD4I CCNGG 4 cut(s) 33, 145, 515, 710
StyI CCWWGG 1 cut(s) 952
TaaI ACNGT 7 cut(s) 78, 121, 414, 464, 503, 694, 913
TaiI ACGT 3 cut(s) 63, 318, 399
TaqI TCGA 2 cut(s) 12, 85
TaqII GACCGA 1 cut(s) 53
TasI AATT 5 cut(s) 110, 236, 373, 926, 966
TatI WGTACW 2 cut(s) 435, 576
TauI GCSGC 3 cut(s) 163, 664, 986
TfiI GAWTC 4 cut(s) 30, 50, 197, 358
Tru1I TTAA 4 cut(s) 213, 471, 707, 743
Tru9I TTAA 4 cut(s) 213, 471, 707, 743
TscAI CASTG 3 cut(s) 467, 619, 697
TseFI GTSAC 2 cut(s) 280, 464
TseI GCWGC 4 cut(s) 273, 391, 687, 962
Tsp45I GTSAC 2 cut(s) 280, 464
TspDTI ATGAA 3 cut(s) 431, 602, 820
TspRI CASTG 3 cut(s) 467, 619, 697
Van91I CCANNNNNTGG 1 cut(s) 78
Vha464I CTTAAG 1 cut(s) 742
VneI GTGCAC 1 cut(s) 458
VpaK11BI GGWCC 4 cut(s) 69, 503, 838, 956
XapI RAATTY 2 cut(s) 236, 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 672
XhoI CTCGAG 1 cut(s) 11
XmiI GTMKAC 1 cut(s) 407
XmnI GAANNNNTTC 2 cut(s) 827, 930
XspI CTAG 3 cut(s) 132, 491, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.