Prupe.3G023600_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001298
Physical Location & Seq
Reverse (-)
1741159 .. 1742289
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G023600.1

Sequence Viewer

Length: 1131 bp
ATGGGTTCATTGAAGAGGAAAAGAGAAACCCAAAAGGAAGACGAACCGGGACTTGTACAGAAAGAGAGGAGTTTGTCAATTCCTGAGTGCGAGTGGGTTCGAGGGCGGATGCTCGGCAAAGGCGGATTCGGGTCGGTTTATTTGGCCACTGCGAAAAAACCCAAGTTGGGTAGCGAGGATTTGCCGGCGATTATGGCTGTGAAATCAGGACGGGTCTCTAAATCGCCTGAGCTGCTCATGGAAAGGACACTTCTTAAAATTTTTGGCGACTGCCCTTTTGTTATCGACTGTTATGGATCAGATATGACCGCTGATGCTGAAAATAAATGTACATACAATGTGTTCATGGAGTATGCTGATGGAGGAACGATGAGGGATTTGATAAAGAAATCCAGGGGTTGCGGGTTGCTTGAGCCCCAAGTAAGAAAGTATACAGGGTGTCTTCTGAAAGGGCTTCAGTACATTCATGTAAGGGGCTGTGTCCACTGTGATCTGAAGCCTGAAAACATATTGCTTGTGAGTAACAGTGATGGTGATTTTGTGCCCAAAATTGGGGACTTCGGACTGGCAAAGTTTGCTGTTTATAAAAAAAAGAGTCGCAAGGAACCTACTTGTCAAGGCACTGCAATATATTTGTCACCAGAAGCCGTGCTTTATGGAAATCAGGAGAAGCCTTCTGATATTTGGGCCTTGGGTTGTGTTGTGCTCGAGATGTTAACAGGGAGGTGGCCGTGGGATTTGGAAGCCGGTGCGACACTTCAAGACCTACAGCTTCTGATTGCTTCAAAAGTTCCCACTGTTCCTGGCTGGCTATCAGAAGATGCTAAAGATTTCTTGCGAAAGTGCTTTGTGAGGAACCCTTCTGAGAGGTTGGAAGCTGCTAAGCTATTGAACCACCCTTTTGTGACCAAACTTGATGGGCTTGGAGAGGTCAAAGTTGAGCCATTAAAGAAGCAAGTCTCTGCAGTGCCATCCTCTGAGAATTGTGAAAAGGCAGAAGGTTCAAAACCTGGGGATGCAGAGGAGATTCTTCCCTTGGCAGTCCTGTACCCTGGGGACTCAAAACCTGTGATTCTTCCAACCACTGTTTGCAGAAAGCCATCAAATTTTGGTATAACTGGTGCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

377

Amino Acids

41.28

Weight (kDa)

8.44

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AccI GTMKAC 1 cut(s) 431
AciI CCGC 4 cut(s) 106, 123, 309, 402
AclWI GGATC 1 cut(s) 304
AcoI YGGCCR 2 cut(s) 144, 728
AcsI RAATTY 2 cut(s) 258, 1105
AcuI CTGAAG 2 cut(s) 440, 515
AfaI GTAC 4 cut(s) 57, 331, 461, 1049
AfiI CCNNNNNNNGG 3 cut(s) 167, 551, 552
AgsI TTSAA 5 cut(s) 13, 761, 786, 892, 1005
AjnI CCWGG 4 cut(s) 392, 802, 1009, 1051
AluBI AGCT 5 cut(s) 232, 772, 878, 886, 1127
AluI AGCT 5 cut(s) 232, 772, 878, 886, 1127
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 2 cut(s) 220, 964
AlwI GGATC 1 cut(s) 304
AlwNI CAGNNNCTG 1 cut(s) 775
Ama87I CYCGRG 1 cut(s) 707
AoxI GGCC 3 cut(s) 144, 687, 728
ApeKI GCWGC 3 cut(s) 232, 878, 1124
ApoI RAATTY 2 cut(s) 258, 1105
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AspS9I GGNCC 1 cut(s) 687
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 2 cut(s) 545, 630
AvaI CYCGRG 1 cut(s) 707
BaeGI GKGCMC 1 cut(s) 546
BalI TGGCCA 1 cut(s) 146
BanII GRGCYC 1 cut(s) 417
BbsI GAAGAC 2 cut(s) 45, 434
Bbv12I GWGCWC 1 cut(s) 708
BbvI GCAGC 2 cut(s) 219, 865
BccI CCATC 5 cut(s) 353, 524, 911, 979, 1108
BceAI ACGGC 2 cut(s) 632, 715
BciT130I CCWGG 4 cut(s) 394, 804, 1011, 1053
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 2 cut(s) 220, 964
BfmI CTRYAG 2 cut(s) 767, 963
BisI GCNGC 3 cut(s) 233, 879, 1125
BlpI GCTNAGC 1 cut(s) 882
BlsI GCNGC 3 cut(s) 234, 880, 1126
Bme1390I CCNGG 5 cut(s) 48, 394, 804, 1011, 1053
BmeT110I CYCGRG 1 cut(s) 707
BmgT120I GGNCC 1 cut(s) 687
BmiI GGNNCC 2 cut(s) 606, 857
BmrFI CCNGG 5 cut(s) 48, 394, 804, 1011, 1053
BmsI GCATC 4 cut(s) 99, 304, 811, 1006
BpiI GAAGAC 2 cut(s) 45, 434
Bpu10I CCTNAGC 1 cut(s) 228
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 7 cut(s) 393, 690, 731, 1010, 1035, 1051, 1052
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 551, 552
Bse118I RCCGGY 2 cut(s) 184, 746
Bse1I ACTGG 2 cut(s) 570, 1123
BseBI CCWGG 4 cut(s) 394, 804, 1011, 1053
BseDI CCNNGG 7 cut(s) 393, 690, 731, 1010, 1035, 1051, 1052
BseGI GGATG 3 cut(s) 114, 971, 1021
BseLI CCNNNNNNNGG 3 cut(s) 167, 551, 552
BseMII CTCAG 4 cut(s) 75, 219, 855, 969
BseNI ACTGG 2 cut(s) 570, 1123
BseRI GAGGAG 2 cut(s) 82, 1037
BseSI GKGCMC 1 cut(s) 546
BseXI GCAGC 2 cut(s) 219, 865
BshFI GGCC 3 cut(s) 146, 689, 730
BsiHKAI GWGCWC 1 cut(s) 708
BsiHKCI CYCGRG 1 cut(s) 707
BsiSI CCGG 3 cut(s) 47, 185, 747
BslFI GGGAC 3 cut(s) 63, 569, 1070
BslI CCNNNNNNNGG 3 cut(s) 167, 551, 552
BsmAI GTCTC 2 cut(s) 220, 964
BsmFI GGGAC 3 cut(s) 63, 569, 1070
BsnI GGCC 3 cut(s) 146, 689, 730
Bso31I GGTCTC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 707
Bsp1286I GDGCHC 3 cut(s) 417, 546, 708
Bsp1407I TGTACA 2 cut(s) 55, 329
Bsp143I GATC 2 cut(s) 296, 490
Bsp1720I GCTNAGC 1 cut(s) 882
BspACI CCGC 4 cut(s) 106, 123, 309, 402
BspANI GGCC 3 cut(s) 146, 689, 730
BspCNI CTCAG 4 cut(s) 76, 220, 856, 970
BspLI GGNNCC 2 cut(s) 606, 857
BspMAI CTGCAG 1 cut(s) 967
BspPI GGATC 1 cut(s) 304
BspTNI GGTCTC 1 cut(s) 220
BsrFI RCCGGY 2 cut(s) 184, 746
BsrGI TGTACA 2 cut(s) 55, 329
BsrI ACTGG 2 cut(s) 570, 1123
BssAI RCCGGY 2 cut(s) 184, 746
BssECI CCNNGG 7 cut(s) 393, 690, 731, 1010, 1035, 1051, 1052
BssMI GATC 2 cut(s) 296, 490
BssNAI GTATAC 1 cut(s) 432
BssT1I CCWWGG 2 cut(s) 690, 1035
Bst1107I GTATAC 1 cut(s) 432
Bst2UI CCWGG 4 cut(s) 394, 804, 1011, 1053
Bst4CI ACNGT 5 cut(s) 290, 488, 527, 799, 1087
Bst6I CTCTTC 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 575
BstAUI TGTACA 2 cut(s) 55, 329
BstC8I GCNNGC 2 cut(s) 186, 809
BstDEI CTNAG 5 cut(s) 84, 228, 864, 882, 978
BstDSI CCRYGG 1 cut(s) 731
BstF5I GGATG 3 cut(s) 114, 971, 1021
BstKTI GATC 2 cut(s) 299, 493
BstMAI GTCTC 2 cut(s) 220, 964
BstMBI GATC 2 cut(s) 296, 490
BstMWI GCNNNNNNNGC 3 cut(s) 194, 232, 575
BstNI CCWGG 4 cut(s) 394, 804, 1011, 1053
BstSCI CCNGG 5 cut(s) 46, 392, 802, 1009, 1051
BstSFI CTRYAG 2 cut(s) 767, 963
BstSLI GKGCMC 1 cut(s) 546
BstV1I GCAGC 2 cut(s) 219, 865
BstV2I GAAGAC 2 cut(s) 45, 434
BstZ17I GTATAC 1 cut(s) 432
BsuRI GGCC 3 cut(s) 146, 689, 730
BtgI CCRYGG 1 cut(s) 731
BtsCI GGATG 3 cut(s) 114, 971, 1021
BtsI GCAGTG 3 cut(s) 147, 621, 972
BtsIMutI CAGTG 7 cut(s) 147, 484, 532, 621, 795, 972, 1083
Cac8I GCNNGC 2 cut(s) 186, 809
CaiI CAGNNNCTG 1 cut(s) 775
Cfr10I RCCGGY 2 cut(s) 184, 746
Cfr13I GGNCC 1 cut(s) 687
Csp6I GTAC 4 cut(s) 56, 330, 460, 1048
CviAII CATG 3 cut(s) 238, 346, 467
CviQI GTAC 4 cut(s) 56, 330, 460, 1048
DdeI CTNAG 5 cut(s) 84, 228, 864, 882, 978
DpnI GATC 2 cut(s) 298, 492
DpnII GATC 2 cut(s) 296, 490
EaeI YGGCCR 2 cut(s) 144, 728
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
EciI GGCGGA 2 cut(s) 121, 138
Eco130I CCWWGG 2 cut(s) 690, 1035
Eco24I GRGCYC 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 220
Eco57I CTGAAG 2 cut(s) 440, 515
Eco88I CYCGRG 1 cut(s) 707
EcoRII CCWGG 4 cut(s) 392, 802, 1009, 1051
EcoT14I CCWWGG 2 cut(s) 690, 1035
EcoT38I GRGCYC 1 cut(s) 417
ErhI CCWWGG 2 cut(s) 690, 1035
FaeI CATG 3 cut(s) 241, 349, 470
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 3 cut(s) 63, 569, 1070
FatI CATG 3 cut(s) 237, 345, 466
FauI CCCGC 1 cut(s) 395
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 3 cut(s) 233, 879, 1125
FokI GGATG 3 cut(s) 121, 958, 1028
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 3 cut(s) 233, 879, 1125
GluI GCNGC 3 cut(s) 233, 879, 1125
HaeIII GGCC 3 cut(s) 146, 689, 730
HapII CCGG 3 cut(s) 47, 185, 747
Hin1II CATG 3 cut(s) 241, 349, 470
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 5 cut(s) 126, 595, 1027, 1058, 1072
HpaI GTTAAC 1 cut(s) 717
HpaII CCGG 3 cut(s) 47, 185, 747
HphI GGTGA 2 cut(s) 545, 630
Hpy166II GTNNAC 3 cut(s) 432, 484, 717
Hpy188I TCNGA 9 cut(s) 301, 447, 495, 563, 679, 777, 817, 865, 979
Hpy188III TCNNGA 5 cut(s) 83, 207, 665, 709, 761
Hpy8I GTNNAC 3 cut(s) 432, 484, 717
HpyAV CCTTC 3 cut(s) 684, 870, 992
HpyCH4III ACNGT 5 cut(s) 290, 488, 527, 799, 1087
HpyCH4V TGCA 5 cut(s) 626, 965, 1019, 1092, 1124
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 232, 575
HpyF3I CTNAG 5 cut(s) 84, 228, 864, 882, 978
Hsp92II CATG 3 cut(s) 241, 349, 470
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
KspAI GTTAAC 1 cut(s) 717
Kzo9I GATC 2 cut(s) 296, 490
Lsp1109I GCAGC 2 cut(s) 219, 865
LweI GCATC 4 cut(s) 99, 304, 811, 1006
MaeIII GTNAC 3 cut(s) 521, 636, 904
MalI GATC 2 cut(s) 298, 492
MboI GATC 2 cut(s) 296, 490
MboII GAAGA 6 cut(s) 25, 50, 434, 830, 1022, 1067
MhlI GDGCHC 3 cut(s) 417, 546, 708
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 5 cut(s) 78, 258, 549, 982, 1105
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 2 cut(s) 604, 1052
MmeI TCCRAC 2 cut(s) 852, 1103
Mox20I TGGCCA 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 184
MscI TGGCCA 1 cut(s) 146
MseI TTAA 4 cut(s) 255, 716, 947, 1129
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 3 cut(s) 47, 185, 747
MspR9I CCNGG 5 cut(s) 48, 394, 804, 1011, 1053
MvaI CCWGG 4 cut(s) 394, 804, 1011, 1053
MwoI GCNNNNNNNGC 3 cut(s) 194, 232, 575
NaeI GCCGGC 1 cut(s) 186
NciI CCSGG 1 cut(s) 48
NdeII GATC 2 cut(s) 296, 490
NgoMIV GCCGGC 1 cut(s) 184
NlaIII CATG 3 cut(s) 241, 349, 470
NlaIV GGNNCC 2 cut(s) 606, 857
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 2 cut(s) 636, 904
PaeR7I CTCGAG 1 cut(s) 707
PasI CCCWGGG 1 cut(s) 1052
PdiI GCCGGC 1 cut(s) 186
PfeI GAWTC 3 cut(s) 126, 1027, 1072
PkrI GCNGC 3 cut(s) 234, 880, 1126
PleI GAGTC 2 cut(s) 603, 1052
PpsI GAGTC 2 cut(s) 603, 1052
PsiI TTATAA 1 cut(s) 585
Psp6I CCWGG 4 cut(s) 392, 802, 1009, 1051
PspGI CCWGG 4 cut(s) 392, 802, 1009, 1051
PspN4I GGNNCC 2 cut(s) 606, 857
PspPI GGNCC 1 cut(s) 687
PstI CTGCAG 1 cut(s) 967
PstNI CAGNNNCTG 1 cut(s) 775
RsaI GTAC 4 cut(s) 57, 331, 461, 1049
RsaNI GTAC 4 cut(s) 56, 330, 460, 1048
SaqAI TTAA 4 cut(s) 255, 716, 947, 1129
SatI GCNGC 3 cut(s) 233, 879, 1125
Sau3AI GATC 2 cut(s) 296, 490
Sau96I GGNCC 1 cut(s) 687
SchI GAGTC 2 cut(s) 604, 1052
ScrFI CCNGG 5 cut(s) 48, 394, 804, 1011, 1053
SduI GDGCHC 3 cut(s) 417, 546, 708
SfaNI GCATC 4 cut(s) 99, 304, 811, 1006
SfcI CTRYAG 2 cut(s) 767, 963
Sfr274I CTCGAG 1 cut(s) 707
SlaI CTCGAG 1 cut(s) 707
SmlI CTYRAG 2 cut(s) 410, 707
SmoI CTYRAG 2 cut(s) 410, 707
Sse9I AATT 5 cut(s) 78, 258, 549, 982, 1105
SsiI CCGC 4 cut(s) 106, 123, 309, 402
StyD4I CCNGG 5 cut(s) 46, 392, 802, 1009, 1051
StyI CCWWGG 2 cut(s) 690, 1035
TaaI ACNGT 5 cut(s) 290, 488, 527, 799, 1087
TaqI TCGA 3 cut(s) 100, 285, 708
TasI AATT 5 cut(s) 78, 258, 549, 982, 1105
TatI WGTACW 3 cut(s) 55, 329, 459
TfiI GAWTC 3 cut(s) 126, 1027, 1072
Tru1I TTAA 4 cut(s) 255, 716, 947, 1129
Tru9I TTAA 4 cut(s) 255, 716, 947, 1129
TscAI CASTG 7 cut(s) 154, 491, 532, 628, 802, 972, 1090
TseFI GTSAC 2 cut(s) 636, 904
TseI GCWGC 3 cut(s) 232, 878, 1124
Tsp45I GTSAC 2 cut(s) 636, 904
TspDTI ATGAA 2 cut(s) 334, 455
TspRI CASTG 7 cut(s) 154, 491, 532, 628, 802, 972, 1090
XapI RAATTY 2 cut(s) 258, 1105
XhoI CTCGAG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.