RLG00000008574

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001298
Physical Location & Seq
Reverse (-)
32346061 .. 32349856
3796 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008574

Sequence Viewer

Length: 1188 bp
ATGGCCGTTAAGGTCGCTGAGGTTTCTGATCCAAAAAAGGTTGATGCTTTATTCACCGAGGCCAAACTTCTCTCTCAGTTTCAGGACTGCCCTTTTGTTATCAACTCTTTTGGAGAACAGTGGACTTATAAGTCTCATCATAATTCTTTCTCTTTCAATCTGCTATTAGAATTTGCGGCCGGAGGCACTATAAGAGATAGGATTCAGAAATCCGCATCCGGCTTGTCCGAATGTGAGATAAAAGAGTTCACCAAGTCAGTTCTTAGTGGTCTCCAGTGCATTCATGACAAAGGTTATGTGCATTGCGACATAAAGCCCGATAACATTCTTCTTGTGCACGACATGACTTCTCATGTCGATTTTGTGGCTAAGATAGGAGACTTTGGATTGACAAAGAGCGCTCAAAATGAGTATTCTCGCCAAGGCACCGCAAGCTATTTGCCTCCTGAAACTGTTATCAGTAACATTCAAGGGAAGCCATCAGACATTTGGGCACTAGGATGTTTAGTTCTAGAGATGTTGACTGGAACCTTGTGGCCTTGCAAGACAAGTGAGGACTTAGGCGGTTGGGTTTCAAATCCAATTATTCCTTCACACTTGTCCGAGGAGGCCAAGGATTTTTTGGCAAAGTGTCTGGATATAGATGCTTCCAAGAGATTTACGGCTGAGAGGCTCTTGACTCACTCATTTCTTATGAGTGAACAGAGCATTGAACAAGTAGCTAAAGTGCCCGCATTGTCACAATCTCAGCATTTATGTGATCGCAAAAAGGGGAAGTCTGGAGTAGTGGTGCGAGATGAAGAAGGAAGGCTCAAACTAGGTAATCCTTCAGTTCAAGGCTTCAAGCAATCGAAAACCCTTGCATATGGTCTGAATGAAATTAGCTGGAGTGAAAACTCAAACCAGTGGAGTTGGAGAGCTATGCAGCCAATGTCATTTGGGCTCTCTAATAAAATTGGCTGCATTGCTCCCCAACTCCACTGCTTTGAAGTTTTCACTCCAGCTAATTTCATTTATAAGATTGGCTGCATTGCTCTCCAATCTGTCTTAAGACGGATATGGAAGCGGCAAATTTCGATAAGATTGGATCTCGTGATGCCAGAGTTTCGTAGCTATTACATGGAGAAAGATCCCTCGAGATTGCAAACCAAGCTGCACTCATTCAGTGCGAAGAAAGGAAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

396

Amino Acids

44.35

Weight (kDa)

8.53

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 231 8.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 2 - 226 4.3e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 129, 1017
AasI GACNNNNNNGTC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 425
AciI CCGC 6 cut(s) 176, 213, 429, 564, 732, 1066
AclWI GGATC 3 cut(s) 23, 1095, 1124
AcoI YGGCCR 2 cut(s) 3, 177
AcsI RAATTY 2 cut(s) 170, 1071
AcuI CTGAAG 1 cut(s) 813
AfeI AGCGCT 1 cut(s) 400
AflII CTTAAG 1 cut(s) 1048
AgsI TTSAA 7 cut(s) 157, 470, 576, 713, 836, 844, 989
AjuI GAANNNNNNNTTGG 2 cut(s) 574, 606
AloI GAACNNNNNNTCC 2 cut(s) 492, 524
AluBI AGCT 8 cut(s) 435, 722, 885, 920, 1004, 1113, 1153, 1183
AluI AGCT 8 cut(s) 435, 722, 885, 920, 1004, 1113, 1153, 1183
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 3 cut(s) 138, 275, 372
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 3 cut(s) 23, 1095, 1124
Ama87I CYCGRG 1 cut(s) 1135
Aor51HI AGCGCT 1 cut(s) 400
AoxI GGCC 5 cut(s) 3, 60, 177, 536, 609
ApaLI GTGCAC 1 cut(s) 335
ApeKI GCWGC 4 cut(s) 925, 960, 1026, 1153
ApoI RAATTY 2 cut(s) 170, 1071
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspLEI GCGC 1 cut(s) 401
AsuHPI GGTGA 2 cut(s) 46, 241
AvaI CYCGRG 1 cut(s) 1135
BaeGI GKGCMC 3 cut(s) 339, 496, 732
BanI GGYRCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 945
BauI CACGAG 1 cut(s) 1091
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 4 cut(s) 937, 947, 1013, 1140
BccI CCATC 1 cut(s) 487
BceAI ACGGC 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 138, 275, 372
BfaI CTAG 3 cut(s) 497, 512, 818
BfoI RGCGCY 1 cut(s) 402
BfrI CTTAAG 1 cut(s) 1048
BisI GCNGC 6 cut(s) 177, 926, 961, 1027, 1067, 1154
BlsI GCNGC 6 cut(s) 178, 927, 962, 1028, 1068, 1155
BmeT110I CYCGRG 1 cut(s) 1135
BmiI GGNNCC 2 cut(s) 427, 529
BmsI GCATC 4 cut(s) 34, 224, 634, 1086
BpmI CTGGAG 4 cut(s) 257, 801, 907, 984
Bpu10I CCTNAGC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 4 cut(s) 57, 421, 603, 612
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bse1I ACTGG 3 cut(s) 274, 529, 904
Bse3DI GCAATG 3 cut(s) 301, 963, 1029
BseDI CCNNGG 4 cut(s) 57, 421, 603, 612
BseGI GGATG 2 cut(s) 215, 506
BseMI GCAATG 3 cut(s) 301, 963, 1029
BseMII CTCAG 4 cut(s) 9, 89, 657, 761
BseNI ACTGG 3 cut(s) 274, 529, 904
BseRI GAGGAG 1 cut(s) 620
BseSI GKGCMC 3 cut(s) 339, 496, 732
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 4 cut(s) 937, 947, 1013, 1140
BsgI GTGCAG 1 cut(s) 1139
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 5 cut(s) 5, 62, 179, 538, 611
BshNI GGYRCC 1 cut(s) 425
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 1135
BsiSI CCGG 2 cut(s) 180, 219
BsmAI GTCTC 3 cut(s) 138, 275, 372
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 5 cut(s) 5, 62, 179, 538, 611
Bso31I GGTCTC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 1135
Bsp1286I GDGCHC 4 cut(s) 339, 496, 732, 945
Bsp143I GATC 4 cut(s) 28, 760, 1087, 1129
BspACI CCGC 6 cut(s) 176, 213, 429, 564, 732, 1066
BspANI GGCC 5 cut(s) 5, 62, 179, 538, 611
BspCNI CTCAG 4 cut(s) 10, 88, 658, 760
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 427, 529
BspPI GGATC 3 cut(s) 23, 1095, 1124
BspT107I GGYRCC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 1048
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 3 cut(s) 301, 963, 1029
BsrI ACTGG 3 cut(s) 274, 529, 904
BssECI CCNNGG 4 cut(s) 57, 421, 603, 612
BssMI GATC 4 cut(s) 28, 760, 1087, 1129
BssSI CACGAG 1 cut(s) 1091
BssT1I CCWWGG 2 cut(s) 421, 612
Bst2BI CACGAG 1 cut(s) 1091
Bst4CI ACNGT 2 cut(s) 120, 454
BstAFI CTTAAG 1 cut(s) 1048
BstC8I GCNNGC 2 cut(s) 433, 732
BstDEI CTNAG 7 cut(s) 18, 75, 263, 369, 559, 666, 747
BstF5I GGATG 2 cut(s) 215, 506
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 4 cut(s) 31, 763, 1090, 1132
BstMAI GTCTC 3 cut(s) 138, 275, 372
BstMBI GATC 4 cut(s) 28, 760, 1087, 1129
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 2 cut(s) 432, 1150
BstSLI GKGCMC 3 cut(s) 339, 496, 732
BstV1I GCAGC 4 cut(s) 937, 947, 1013, 1140
BstX2I RGATCY 2 cut(s) 1087, 1129
BstYI RGATCY 2 cut(s) 1087, 1129
BstZI CGGCCG 1 cut(s) 177
BsuRI GGCC 5 cut(s) 5, 62, 179, 538, 611
BtsCI GGATG 2 cut(s) 215, 506
BtsI GCAGTG 1 cut(s) 979
BtsIMutI CAGTG 5 cut(s) 125, 281, 911, 979, 1171
Cac8I GCNNGC 2 cut(s) 433, 732
CciI TCATGA 1 cut(s) 283
CfoI GCGC 1 cut(s) 401
CviAII CATG 4 cut(s) 284, 343, 353, 1120
DdeI CTNAG 7 cut(s) 18, 75, 263, 369, 559, 666, 747
DpnI GATC 4 cut(s) 30, 762, 1089, 1131
DpnII GATC 4 cut(s) 28, 760, 1087, 1129
DrdI GACNNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 130
EaeI YGGCCR 2 cut(s) 3, 177
EagI CGGCCG 1 cut(s) 177
EclXI CGGCCG 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 421, 612
Eco24I GRGCYC 1 cut(s) 945
Eco31I GGTCTC 1 cut(s) 275
Eco47III AGCGCT 1 cut(s) 400
Eco52I CGGCCG 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 813
Eco88I CYCGRG 1 cut(s) 1135
EcoT14I CCWWGG 2 cut(s) 421, 612
EcoT38I GRGCYC 1 cut(s) 945
ErhI CCWWGG 2 cut(s) 421, 612
FaeI CATG 4 cut(s) 287, 346, 356, 1123
FalI AAGNNNNNCTT 1 cut(s) 1167
FatI CATG 4 cut(s) 283, 342, 352, 1119
FauI CCCGC 1 cut(s) 739
FauNDI CATATG 1 cut(s) 865
Fnu4HI GCNGC 6 cut(s) 177, 926, 961, 1027, 1067, 1154
FokI GGATG 2 cut(s) 202, 513
FriOI GRGCYC 1 cut(s) 945
Fsp4HI GCNGC 6 cut(s) 177, 926, 961, 1027, 1067, 1154
FspBI CTAG 3 cut(s) 497, 512, 818
GlaI GCGC 1 cut(s) 400
GluI GCNGC 6 cut(s) 177, 926, 961, 1027, 1067, 1154
GsuI CTGGAG 4 cut(s) 257, 801, 907, 984
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 5 cut(s) 5, 62, 179, 538, 611
HapII CCGG 2 cut(s) 180, 219
HhaI GCGC 1 cut(s) 401
Hin1II CATG 4 cut(s) 287, 346, 356, 1123
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 1181
HinfI GANTC 2 cut(s) 202, 679
HpaII CCGG 2 cut(s) 180, 219
HphI GGTGA 2 cut(s) 46, 241
Hpy166II GTNNAC 5 cut(s) 123, 249, 337, 522, 701
Hpy188I TCNGA 6 cut(s) 28, 207, 229, 484, 604, 873
Hpy188III TCNNGA 9 cut(s) 83, 284, 446, 512, 635, 676, 780, 1093, 1137
Hpy8I GTNNAC 5 cut(s) 123, 249, 337, 522, 701
HpyAV CCTTC 4 cut(s) 600, 797, 801, 837
HpyCH4III ACNGT 2 cut(s) 120, 454
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 1150
HpyF3I CTNAG 7 cut(s) 18, 75, 263, 369, 559, 666, 747
Hsp92II CATG 4 cut(s) 287, 346, 356, 1123
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 4 cut(s) 28, 760, 1087, 1129
LmnI GCTCC 1 cut(s) 973
Lsp1109I GCAGC 4 cut(s) 937, 947, 1013, 1140
LweI GCATC 4 cut(s) 34, 224, 634, 1086
MaeI CTAG 3 cut(s) 497, 512, 818
MaeIII GTNAC 2 cut(s) 461, 738
MalI GATC 4 cut(s) 30, 762, 1089, 1131
MboI GATC 4 cut(s) 28, 760, 1087, 1129
MboII GAAGA 3 cut(s) 320, 812, 1183
MflI RGATCY 2 cut(s) 1087, 1129
MhlI GDGCHC 4 cut(s) 339, 496, 732, 945
MluCI AATT 7 cut(s) 142, 170, 582, 879, 954, 1006, 1071
MlyI GAGTC 1 cut(s) 673
MmeI TCCRAC 1 cut(s) 893
MnlI CCTC 9 cut(s) 13, 52, 176, 453, 547, 598, 601, 663, 1144
MseI TTAA 2 cut(s) 9, 1049
MslI CAYNNNNRTG 2 cut(s) 499, 756
MspCI CTTAAG 1 cut(s) 1048
MspI CCGG 2 cut(s) 180, 219
Mva1269I GAATGC 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 432, 1150
NdeI CATATG 1 cut(s) 865
NdeII GATC 4 cut(s) 28, 760, 1087, 1129
NlaIII CATG 4 cut(s) 287, 346, 356, 1123
NlaIV GGNNCC 2 cut(s) 427, 529
NmuCI GTSAC 1 cut(s) 738
PaeR7I CTCGAG 1 cut(s) 1135
PagI TCATGA 1 cut(s) 283
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 6 cut(s) 178, 927, 962, 1028, 1068, 1155
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
PsiI TTATAA 2 cut(s) 129, 1017
PspN4I GGNNCC 2 cut(s) 427, 529
PsuI RGATCY 2 cut(s) 1087, 1129
RseI CAYNNNNRTG 2 cut(s) 499, 756
SaqAI TTAA 2 cut(s) 9, 1049
SatI GCNGC 6 cut(s) 177, 926, 961, 1027, 1067, 1154
Sau3AI GATC 4 cut(s) 28, 760, 1087, 1129
SchI GAGTC 1 cut(s) 673
SduI GDGCHC 4 cut(s) 339, 496, 732, 945
SfaNI GCATC 4 cut(s) 34, 224, 634, 1086
Sfr274I CTCGAG 1 cut(s) 1135
SlaI CTCGAG 1 cut(s) 1135
SmiMI CAYNNNNRTG 2 cut(s) 499, 756
SmlI CTYRAG 2 cut(s) 1048, 1135
SmoI CTYRAG 2 cut(s) 1048, 1135
Sse9I AATT 7 cut(s) 142, 170, 582, 879, 954, 1006, 1071
SsiI CCGC 6 cut(s) 176, 213, 429, 564, 732, 1066
SspMI CTAG 3 cut(s) 497, 512, 818
StyI CCWWGG 2 cut(s) 421, 612
TaaI ACNGT 2 cut(s) 120, 454
TaqI TCGA 4 cut(s) 357, 851, 1076, 1136
TasI AATT 7 cut(s) 142, 170, 582, 879, 954, 1006, 1071
TauI GCSGC 2 cut(s) 179, 1069
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 2 cut(s) 9, 1049
Tru9I TTAA 2 cut(s) 9, 1049
TscAI CASTG 5 cut(s) 125, 281, 911, 986, 1171
TseFI GTSAC 1 cut(s) 738
TseI GCWGC 4 cut(s) 925, 960, 1026, 1153
Tsp45I GTSAC 1 cut(s) 738
TspDTI ATGAA 4 cut(s) 272, 813, 891, 1000
TspGWI ACGGA 1 cut(s) 1069
TspRI CASTG 5 cut(s) 125, 281, 911, 986, 1171
Vha464I CTTAAG 1 cut(s) 1048
VneI GTGCAC 1 cut(s) 335
XapI RAATTY 2 cut(s) 170, 1071
XbaI TCTAGA 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 2 cut(s) 486, 619
XhoI CTCGAG 1 cut(s) 1135
XspI CTAG 3 cut(s) 497, 512, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.