FvH4_7g30023

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000970
Physical Location & Seq
Reverse (-)
21961401 .. 21965405
4005 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30023.t4

Sequence Viewer

Length: 1464 bp
ATGGAGGAGCCGCTGCTGAACAGCGGAGCCGTCGTGGACTACGAGCCGGTGACAAGCTTCAAGCAGGCTAGGGAGATCGCATGGAAAGAAACAGTGAAGCTATGGAGGATTGGAGGGCCTATAGCCTTCACCATAATCTGCAACTTCGGCACCAACTCCGTCTCCACCATGTTCGTCGGACATATCGGAAACCTTGAGCTCTCCGCCGTCTCTATCTCTCTCTCCGTCATCAGCACCTTCTCTTTCGGCTTCCTGCTTGGTATGGGAAGTGCACTAGAAACATTATGTGGACAAGCATTTGGAGCTGGACGTGTTAACATGCTTGGTATCTACATGCAAAGGTCATGGATCATTCTATGGACTACCTGCATAATCCTCACGCCCCTCTATTGCTTCTCTGCGCCAATCTTAAAGCTTCTAGGCCAAGATGCTGATGTATCCGACCTCGCAGGGACATTCACCGTGTATGTGATCCCTCAGCTATTCTCACTTTCCATCAATTTCCCTTGCCAAAAGTTCCTGCAGGCACAAAGCAAGGTCTTGGTCCTTGCTTGGATCGCATTCATCGCCCTGATGATTCACATTGGACTTCTTTACCTCTTCATTTATGTGTTGGATTGGGGGATATATGGTGCAGCTGTAGCTTATGACATTACAGGTTGGGAACTTGTGATTGCTCAGCTTATTTATATATTTGGTTGGTGTAACGAAGGGTGGAGCGGGTTTTCATGGCTGGCGTTCAAAGACATTTGGGCCTTTGTGAAGCTTTCTCTTGAATCGGCTGTCATGCTTTGCCTTGAGATTTGGTATATGATGAGTATCATCATTCTTACTGGCCATCTTGACAATGCTGTCATCTCTCTTGATTCGCTTTCTATTTGCATGAATGTCAATGGATGGGAAGCAATGCTGTTCATTGGAATCAATGCTGCTATTAGTGTTCGAGTTTCTAATGAACTTGGATTGGGGCGTCCAAGAGCAGCTAAATACTCGGTCTATGTGACAGTGGCTCAGTCTCTGCTGATTGGAATTCTCTGCATGGTTATCATCTTGATTTCCAAAGACTACTTCTCTGTCATCTTCACTAGCAATGCTGAATTGCAAGCAGCTGTTTCAAAGCTAGCATGGCTTCTCGGAATCACTATGGTTCTCAACAGTGTCCAGCCTGTGATTTCAGGTGTTGCTATCGGAGGCGGATGGCAAGGAATTGTGGCTTATATCAACTTGGGTTGTTACTACATTTTTGGGCTCCCATTTGGATTCATTCTTGGTTATGTTGTAGGCTGGGGAGTGACAGGGATTTGGGGAGGCATGATATGTGGAACAGCTCTTCAAACATTGCTGCTCGTTATAATACTTTACAGAACCAACTGGAACAAGGAGGTGGAAGAAAGTTCGTCCCGAATGAAGAGATGGGGTGGACAAGATATAAAAGGCGAGACGACAGCTGATAGTATTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

488

Amino Acids

53.49

Weight (kDa)

5.21

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 40 - 200 2.5e-32 MatE
MatE PF01554 261 - 396 1.7e-16 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1352
AasI GACNNNNNNGTC 1 cut(s) 851
Acc36I ACCTGC 1 cut(s) 374
AccB1I GGYRCC 1 cut(s) 149
AccBSI CCGCTC 1 cut(s) 720
AciI CCGC 5 cut(s) 11, 24, 204, 720, 1194
AclWI GGATC 3 cut(s) 356, 466, 563
AcoI YGGCCR 1 cut(s) 835
AcsI RAATTY 1 cut(s) 1029
AcyI GRCGYC 1 cut(s) 970
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 5 cut(s) 61, 742, 776, 1116, 1334
AjiI CACGTC 1 cut(s) 311
AjuI GAANNNNNNNTTGG 2 cut(s) 1052, 1084
Alw21I GWGCWC 2 cut(s) 201, 274
Alw26I GTCTC 4 cut(s) 166, 214, 1020, 1433
Alw44I GTGCAC 1 cut(s) 270
AlwI GGATC 3 cut(s) 356, 466, 563
AlwNI CAGNNNCTG 1 cut(s) 1018
AoxI GGCC 4 cut(s) 116, 421, 753, 835
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 6 cut(s) 13, 635, 929, 980, 1106, 1342
ApoI RAATTY 1 cut(s) 1029
AspLEI GCGC 1 cut(s) 403
AspS9I GGNCC 3 cut(s) 116, 544, 753
AsuHPI GGTGA 3 cut(s) 61, 121, 451
AsuNHI GCTAGC 1 cut(s) 1120
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 274
BalI TGGCCA 1 cut(s) 837
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 2 cut(s) 201, 1251
Bbv12I GWGCWC 2 cut(s) 201, 274
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 5 cut(s) 647, 916, 992, 1118, 1329
BccI CCATC 5 cut(s) 503, 846, 891, 1191, 1407
BceAI ACGGC 2 cut(s) 14, 191
BciVI GTATCC 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 166, 214, 1020, 1433
BfaI CTAG 5 cut(s) 69, 275, 419, 1086, 1121
BfmI CTRYAG 3 cut(s) 120, 521, 639
BfuAI ACCTGC 1 cut(s) 374
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 7 cut(s) 11, 14, 636, 930, 981, 1107, 1343
BlpI GCTNAGC 1 cut(s) 678
BlsI GCNGC 7 cut(s) 12, 15, 637, 931, 982, 1108, 1344
Bme18I GGWCC 1 cut(s) 544
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 3 cut(s) 116, 544, 753
BmiI GGNNCC 4 cut(s) 9, 28, 151, 1250
BmsI GCATC 1 cut(s) 418
BmtI GCTAGC 1 cut(s) 1124
Bpu10I CCTNAGC 1 cut(s) 477
Bpu1102I GCTNAGC 1 cut(s) 678
BpuEI CTTGAG 2 cut(s) 215, 818
BsaBI GATNNNNATC 1 cut(s) 818
BsaHI GRCGYC 1 cut(s) 970
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 146, 176
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 2 cut(s) 838, 1376
Bse3DI GCAATG 3 cut(s) 912, 1096, 1337
Bse8I GATNNNNATC 1 cut(s) 818
BseGI GGATG 2 cut(s) 902, 1202
BseJI GATNNNNATC 1 cut(s) 818
BseMI GCAATG 3 cut(s) 912, 1096, 1337
BseMII CTCAG 3 cut(s) 491, 692, 1025
BseNI ACTGG 2 cut(s) 838, 1376
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 5 cut(s) 647, 916, 992, 1118, 1329
BseYI CCCAGC 1 cut(s) 1284
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 4 cut(s) 118, 423, 755, 837
BshNI GGYRCC 1 cut(s) 149
BsiHKAI GWGCWC 2 cut(s) 201, 274
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 466, 1384
BsmAI GTCTC 4 cut(s) 166, 214, 1020, 1433
BsmBI CGTCTC 3 cut(s) 166, 214, 1433
BsmFI GGGAC 2 cut(s) 466, 1384
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 4 cut(s) 118, 423, 755, 837
Bsp1286I GDGCHC 3 cut(s) 201, 274, 1251
Bsp143I GATC 4 cut(s) 75, 348, 471, 555
Bsp1720I GCTNAGC 1 cut(s) 678
BspACI CCGC 5 cut(s) 11, 24, 204, 720, 1194
BspANI GGCC 4 cut(s) 118, 423, 755, 837
BspCNI CTCAG 3 cut(s) 490, 691, 1024
BspLI GGNNCC 4 cut(s) 9, 28, 151, 1250
BspMAI CTGCAG 1 cut(s) 525
BspMI ACCTGC 1 cut(s) 374
BspOI GCTAGC 1 cut(s) 1124
BspPI GGATC 3 cut(s) 356, 466, 563
BspQI GCTCTTC 1 cut(s) 1335
BspT107I GGYRCC 1 cut(s) 149
BsrBI CCGCTC 1 cut(s) 720
BsrDI GCAATG 3 cut(s) 912, 1096, 1337
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 2 cut(s) 838, 1376
BssAI RCCGGY 1 cut(s) 46
BssMI GATC 4 cut(s) 75, 348, 471, 555
BssNI GRCGYC 1 cut(s) 970
Bst4CI ACNGT 4 cut(s) 94, 463, 1006, 1157
Bst6I CTCTTC 3 cut(s) 605, 1335, 1403
BstACI GRCGYC 1 cut(s) 970
BstC8I GCNNGC 5 cut(s) 66, 525, 735, 1104, 1122
BstDEI CTNAG 3 cut(s) 477, 678, 1011
BstF5I GGATG 2 cut(s) 902, 1202
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 4 cut(s) 78, 351, 474, 558
BstMAI GTCTC 4 cut(s) 166, 214, 1020, 1433
BstMBI GATC 4 cut(s) 75, 348, 471, 555
BstMWI GCNNNNNNNGC 6 cut(s) 147, 302, 557, 566, 641, 1126
BstNSI RCATGY 2 cut(s) 322, 337
BstSFI CTRYAG 3 cut(s) 120, 521, 639
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 5 cut(s) 647, 916, 992, 1118, 1329
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 4 cut(s) 118, 423, 755, 837
BtgZI GCGATG 1 cut(s) 550
BtrI CACGTC 1 cut(s) 311
BtsCI GGATG 2 cut(s) 902, 1202
BtsIMutI CAGTG 3 cut(s) 99, 1011, 1162
BveI ACCTGC 1 cut(s) 374
Cac8I GCNNGC 5 cut(s) 66, 525, 735, 1104, 1122
CaiI CAGNNNCTG 1 cut(s) 1018
CfoI GCGC 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 3 cut(s) 116, 544, 753
CseI GACGC 1 cut(s) 959
DdeI CTNAG 3 cut(s) 477, 678, 1011
DpnI GATC 4 cut(s) 77, 350, 473, 557
DpnII GATC 4 cut(s) 75, 348, 471, 555
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 835
Eam1104I CTCTTC 3 cut(s) 605, 1335, 1403
EarI CTCTTC 3 cut(s) 605, 1335, 1403
EciI GGCGGA 2 cut(s) 193, 1209
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 2 cut(s) 201, 1251
Eco47I GGWCC 1 cut(s) 544
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 1029
EcoT38I GRGCYC 2 cut(s) 201, 1251
Esp3I CGTCTC 3 cut(s) 166, 214, 1433
FaqI GGGAC 2 cut(s) 466, 1384
FauI CCCGC 1 cut(s) 713
Fnu4HI GCNGC 7 cut(s) 11, 14, 636, 930, 981, 1107, 1343
FokI GGATG 2 cut(s) 909, 1209
FriOI GRGCYC 2 cut(s) 201, 1251
Fsp4HI GCNGC 7 cut(s) 11, 14, 636, 930, 981, 1107, 1343
FspBI CTAG 5 cut(s) 69, 275, 419, 1086, 1121
GlaI GCGC 1 cut(s) 402
GluI GCNGC 7 cut(s) 11, 14, 636, 930, 981, 1107, 1343
GsaI CCCAGC 1 cut(s) 1288
HaeIII GGCC 4 cut(s) 118, 423, 755, 837
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 959
HhaI GCGC 1 cut(s) 403
Hin1I GRCGYC 1 cut(s) 970
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 3 cut(s) 55, 413, 764
HinfI GANTC 6 cut(s) 577, 776, 866, 921, 1137, 1260
HpaI GTTAAC 1 cut(s) 316
HpaII CCGG 1 cut(s) 47
HphI GGTGA 3 cut(s) 61, 121, 451
Hpy166II GTNNAC 5 cut(s) 37, 272, 290, 316, 1421
Hpy188I TCNGA 5 cut(s) 179, 188, 442, 1136, 1190
Hpy188III TCNNGA 5 cut(s) 773, 842, 863, 1051, 1401
Hpy8I GTNNAC 5 cut(s) 37, 272, 290, 316, 1421
Hpy99I CGWCG 2 cut(s) 35, 179
HpyAV CCTTC 3 cut(s) 136, 247, 704
HpyCH4III ACNGT 4 cut(s) 94, 463, 1006, 1157
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 9 cut(s) 141, 272, 337, 369, 523, 635, 882, 1038, 1102
HpyF10VI GCNNNNNNNGC 6 cut(s) 147, 302, 557, 566, 641, 1126
HpyF3I CTNAG 3 cut(s) 477, 678, 1011
HpySE526I ACGT 1 cut(s) 310
Hsp92I GRCGYC 1 cut(s) 970
HspAI GCGC 1 cut(s) 401
KspAI GTTAAC 1 cut(s) 316
Kzo9I GATC 4 cut(s) 75, 348, 471, 555
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 5 cut(s) 7, 26, 302, 717, 1254
Lsp1109I GCAGC 5 cut(s) 647, 916, 992, 1118, 1329
LweI GCATC 1 cut(s) 418
MaeI CTAG 5 cut(s) 69, 275, 419, 1086, 1121
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 5 cut(s) 49, 704, 1000, 1232, 1291
MalI GATC 4 cut(s) 77, 350, 473, 557
MbiI CCGCTC 1 cut(s) 720
MboI GATC 4 cut(s) 75, 348, 471, 555
MboII GAAGA 5 cut(s) 592, 1072, 1322, 1400, 1420
MhlI GDGCHC 3 cut(s) 201, 274, 1251
MlsI TGGCCA 1 cut(s) 837
MluCI AATT 4 cut(s) 499, 1029, 1097, 1206
MluNI TGGCCA 1 cut(s) 837
MmeI TCCRAC 3 cut(s) 157, 465, 594
Mox20I TGGCCA 1 cut(s) 837
MscI TGGCCA 1 cut(s) 837
MseI TTAA 2 cut(s) 315, 410
MslI CAYNNNNRTG 1 cut(s) 608
Msp20I TGGCCA 1 cut(s) 837
MspA1I CMGCKG 5 cut(s) 13, 24, 638, 1109, 1448
MspI CCGG 1 cut(s) 47
Mva1269I GAATGC 1 cut(s) 560
MwoI GCNNNNNNNGC 6 cut(s) 147, 302, 557, 566, 641, 1126
NdeII GATC 4 cut(s) 75, 348, 471, 555
NheI GCTAGC 1 cut(s) 1120
NlaIV GGNNCC 4 cut(s) 9, 28, 151, 1250
NmuCI GTSAC 3 cut(s) 49, 1000, 1291
NspI RCATGY 2 cut(s) 322, 337
PciSI GCTCTTC 1 cut(s) 1335
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 560
PfeI GAWTC 6 cut(s) 577, 776, 866, 921, 1137, 1260
PkrI GCNGC 7 cut(s) 12, 15, 637, 931, 982, 1108, 1344
PsiI TTATAA 1 cut(s) 1352
Psp124BI GAGCTC 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 1284
PspN4I GGNNCC 4 cut(s) 9, 28, 151, 1250
PspPI GGNCC 3 cut(s) 116, 544, 753
PstI CTGCAG 1 cut(s) 525
PstNI CAGNNNCTG 1 cut(s) 1018
PvuII CAGCTG 3 cut(s) 638, 1109, 1448
RseI CAYNNNNRTG 1 cut(s) 608
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 1335
SaqAI TTAA 2 cut(s) 315, 410
SatI GCNGC 7 cut(s) 11, 14, 636, 930, 981, 1107, 1343
Sau3AI GATC 4 cut(s) 75, 348, 471, 555
Sau96I GGNCC 3 cut(s) 116, 544, 753
SbfI CCTGCAGG 1 cut(s) 525
SdaI CCTGCAGG 1 cut(s) 525
SduI GDGCHC 3 cut(s) 201, 274, 1251
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 3 cut(s) 120, 521, 639
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 2 cut(s) 194, 797
SmoI CTYRAG 2 cut(s) 194, 797
Sse8387I CCTGCAGG 1 cut(s) 525
Sse9I AATT 4 cut(s) 499, 1029, 1097, 1206
SsiI CCGC 5 cut(s) 11, 24, 204, 720, 1194
SspMI CTAG 5 cut(s) 69, 275, 419, 1086, 1121
SstI GAGCTC 1 cut(s) 201
TaaI ACNGT 4 cut(s) 94, 463, 1006, 1157
TaiI ACGT 1 cut(s) 313
TaqI TCGA 1 cut(s) 943
TaqII GACCGA 1 cut(s) 982
TasI AATT 4 cut(s) 499, 1029, 1097, 1206
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 6 cut(s) 577, 776, 866, 921, 1137, 1260
Tru1I TTAA 2 cut(s) 315, 410
Tru9I TTAA 2 cut(s) 315, 410
TscAI CASTG 3 cut(s) 99, 1011, 1162
TseFI GTSAC 3 cut(s) 49, 1000, 1291
TseI GCWGC 6 cut(s) 13, 635, 929, 980, 1106, 1342
Tsp45I GTSAC 3 cut(s) 49, 1000, 1291
TspDTI ATGAA 8 cut(s) 553, 592, 717, 899, 904, 969, 1252, 1421
TspGWI ACGGA 2 cut(s) 148, 214
TspRI CASTG 3 cut(s) 99, 1011, 1162
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 544
XapI RAATTY 1 cut(s) 1029
XceI RCATGY 2 cut(s) 322, 337
XspI CTAG 5 cut(s) 69, 275, 419, 1086, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.