pycom01g21620

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000970
Physical Location & Seq
Reverse (-)
19567348 .. 19571544
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21620.2

Sequence Viewer

Length: 1473 bp
ATGGAGACGCCACTGCTTCACGCAGTGAGCGCCGGCGGGTTCCATGACTACGAGCCGGTGAGGAGCTTCAAGGATGCTCGGTCGGTGGCGTGGAATGAAACCAAGAAGCTGTGGAAGATTGCAGGGCCCATCGCCTTCACCATCATCTGCAACTATGGCACCAACACTGCTTCCGCCATGTTTGTCGGCCATCTCGGAAACCTAGAGCTCTCCGCCGTCTCCATCTCTCTCTCCGTCATCAGCACCTTCTCATTCGGTTTCATGCTTGGCATGGGGAGTGCATTAGAGACGCTTTGTGGCCAAGCTTACGGGGCCGGACGTGTGCACATGCTCGGCGTTTACATGCAAAGATCATGGATCATTCTGTTCACGAGCTGTGTTGTCCTCACGCCAATCTATGTCTTCTCCGGCCCGATTTTAAAGCTTCTTGGGCAGGAAGATGACGTGGCTAATCTTGCCGGAACATTCACTAGATATTGCATACCGCAGTTGTTCTCACTTGCCTTCAACTTCCCTGCCCAGAAGTTCCTTCAGTCTCAAAGCAAAGTTCTTGTGCTTGCGTGGATTGCATTCATCGCGCTTGTTGTACACATTGGATGGCTTTTTCTATTCATTTACGTGTTCGATTGGGGTATATATGGTGTAGGCATTGCGTTTAGCATGACAGGTTGGGAAACTGTGATTGCGCAAAATATTTACATAATGAACTGGTGCAAGGAAGGGTGGACAGGGTTTTCTTGGTTGGCGTTCAAAGACATATGGGCGTTTGTCCGGCTATCACTTGAGTCGGCCGTGATGCTTTGCCTGGAGATTTGGTATATGATGAGTATAACTCTTGTTACTGGCCACCTTAGTAATGCCGTGCTTTCTATTGATTCGCTCAGTATTTGCTTAAATTTCAACGGTTTGGAAGCAATGTTGTTCATTGGCATCAATGCTGCTATAAGTGTTCGAGTCTCCAACGAACTTGGATTGGGACGTCCAAGAGCAGCTAAATACTCAGTCTACGTGACGGTTGGTCAGTCTCTGCTCTTCGGAATCATTTGCATGATTCTGATCTTGATAACCAAAGACTACTTTGCTGTCCTGTTCACAAGCAGCAAGGAATTGCAGCATTCTGTTTCCAATCTAGCAGTTCTTCTCGGAGTGACTATGATTCTCAATAGCGTCCAACCAGTTATTTCGGGTGTTGCCATCGGAGGCGGATGGCAGGGACTGGTGGCTTATATAAACTTGGGTTCCTACTACATCTTCGGGCTTCCACTCGGTATCGTTCTTGGCTATGTAGCAAATTGGGGAGTGAAGGGAATTTGGGGAGGCATGATATGCGGGACTGCTCTTCAAACATTGGTGCTTGTGATTGTACTTTACAGAACCAACTGGACTAAGGAGGTTAAACAAACAACGGACCGGATGAAGAAGTGGGCCGGGCAGGACTACGGCGACGAGAAGATAGATGTTGTTAGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

491

Amino Acids

53.92

Weight (kDa)

7.99

Isoelectric Point (pI)

24.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 982
Acc16I TGCGCA 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 1005
AccII CGCG 1 cut(s) 578
AciI CCGC 6 cut(s) 36, 174, 213, 485, 1203, 1329
AclWI GGATC 1 cut(s) 365
AcoI YGGCCR 4 cut(s) 187, 298, 789, 844
AcsI RAATTY 2 cut(s) 895, 1308
AcuI CTGAAG 1 cut(s) 515
AcyI GRCGYC 2 cut(s) 8, 979
AdeI CACNNNGTG 1 cut(s) 25
AfaI GTAC 2 cut(s) 588, 1365
AflIII ACRYGT 2 cut(s) 319, 618
AgsI TTSAA 5 cut(s) 70, 508, 751, 901, 1343
AhdI GACNNNNNGTC 1 cut(s) 1017
AjiI CACGTC 2 cut(s) 320, 445
AjnI CCWGG 1 cut(s) 804
AluBI AGCT 7 cut(s) 66, 109, 208, 305, 375, 424, 992
AluI AGCT 7 cut(s) 66, 109, 208, 305, 375, 424, 992
Alw21I GWGCWC 2 cut(s) 210, 327
Alw26I GTCTC 5 cut(s) 223, 281, 540, 961, 1029
Alw44I GTGCAC 1 cut(s) 323
AlwI GGATC 1 cut(s) 365
AlwNI CAGNNNCTG 2 cut(s) 1027, 1216
AoxI GGCC 8 cut(s) 125, 187, 298, 312, 409, 789, 844, 1425
ApaI GGGCCC 1 cut(s) 129
ApaLI GTGCAC 1 cut(s) 323
ApeKI GCWGC 4 cut(s) 938, 989, 1098, 1111
ApoI RAATTY 2 cut(s) 895, 1308
AspLEI GCGC 3 cut(s) 32, 580, 688
AspS9I GGNCC 6 cut(s) 125, 126, 312, 410, 1408, 1425
AsuC2I CCSGG 1 cut(s) 1429
AsuHPI GGTGA 2 cut(s) 70, 130
AvaII GGWCC 1 cut(s) 1408
BaeGI GKGCMC 2 cut(s) 129, 327
BalI TGGCCA 2 cut(s) 300, 846
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 2 cut(s) 129, 210
BauI CACGAG 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 394
Bbv12I GWGCWC 2 cut(s) 210, 327
BbvI GCAGC 4 cut(s) 925, 1001, 1110, 1123
BccI CCATC 7 cut(s) 137, 149, 198, 230, 591, 1200, 1202
BceAI ACGGC 4 cut(s) 200, 776, 845, 1456
BciT130I CCWGG 1 cut(s) 806
BcnI CCSGG 1 cut(s) 1429
BcoDI GTCTC 5 cut(s) 223, 281, 540, 961, 1029
BfaI CTAG 3 cut(s) 203, 471, 1130
BfoI RGCGCY 1 cut(s) 33
BisI GCNGC 4 cut(s) 939, 990, 1099, 1112
BlsI GCNGC 4 cut(s) 940, 991, 1100, 1113
Bme1390I CCNGG 2 cut(s) 806, 1429
Bme18I GGWCC 1 cut(s) 1408
BmeRI GACNNNNNGTC 1 cut(s) 1017
BmgBI CACGTC 2 cut(s) 320, 445
BmgT120I GGNCC 6 cut(s) 125, 126, 312, 410, 1408, 1425
BmiI GGNNCC 5 cut(s) 41, 127, 160, 313, 1240
BmrFI CCNGG 2 cut(s) 806, 1429
BmsI GCATC 3 cut(s) 64, 786, 939
BpiI GAAGAC 1 cut(s) 394
BplI GAGNNNNNCTC 2 cut(s) 817, 849
BpmI CTGGAG 1 cut(s) 827
BpuEI CTTGAG 1 cut(s) 803
BpuMI CCSGG 1 cut(s) 1429
BsaAI YACGTR 2 cut(s) 619, 1009
BsaBI GATNNNNATC 1 cut(s) 1055
BsaHI GRCGYC 2 cut(s) 8, 979
BsaWI WCCGGW 1 cut(s) 1410
BsaXI ACNNNNNCTCC 2 cut(s) 800, 830
Bse118I RCCGGY 2 cut(s) 32, 55
Bse1I ACTGG 5 cut(s) 713, 847, 1175, 1221, 1385
Bse3DI GCAATG 2 cut(s) 648, 921
Bse8I GATNNNNATC 1 cut(s) 1055
BseBI CCWGG 1 cut(s) 806
BseGI GGATG 4 cut(s) 79, 602, 1211, 1419
BseJI GATNNNNATC 1 cut(s) 1055
BseMI GCAATG 2 cut(s) 648, 921
BseMII CTCAG 2 cut(s) 895, 1014
BseNI ACTGG 5 cut(s) 713, 847, 1175, 1221, 1385
BseRI GAGGAG 1 cut(s) 76
BseSI GKGCMC 2 cut(s) 129, 327
BseX3I CGGCCG 1 cut(s) 789
BseXI GCAGC 4 cut(s) 925, 1001, 1110, 1123
Bsh1236I CGCG 1 cut(s) 578
Bsh1285I CGRYCG 2 cut(s) 83, 792
BshFI GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
BshNI GGYRCC 1 cut(s) 158
BsiEI CGRYCG 2 cut(s) 83, 792
BsiHKAI GWGCWC 2 cut(s) 210, 327
BsiSI CCGG 8 cut(s) 33, 56, 315, 408, 459, 772, 1411, 1428
BslFI GGGAC 3 cut(s) 990, 1227, 1345
BsmAI GTCTC 5 cut(s) 223, 281, 540, 961, 1029
BsmBI CGTCTC 2 cut(s) 223, 281
BsmFI GGGAC 3 cut(s) 990, 1227, 1345
BsmI GAATGC 2 cut(s) 569, 1114
BsnI GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
Bsp120I GGGCCC 1 cut(s) 125
Bsp1286I GDGCHC 3 cut(s) 129, 210, 327
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 3 cut(s) 350, 357, 1056
BspACI CCGC 6 cut(s) 36, 174, 213, 485, 1203, 1329
BspANI GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
BspCNI CTCAG 2 cut(s) 894, 1013
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 5 cut(s) 41, 127, 160, 313, 1240
BspPI GGATC 1 cut(s) 365
BspQI GCTCTTC 2 cut(s) 1037, 1344
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 648, 921
BsrFI RCCGGY 2 cut(s) 32, 55
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 5 cut(s) 713, 847, 1175, 1221, 1385
BssAI RCCGGY 2 cut(s) 32, 55
BssMI GATC 3 cut(s) 350, 357, 1056
BssNI GRCGYC 2 cut(s) 8, 979
BssSI CACGAG 1 cut(s) 370
Bst2BI CACGAG 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 806
Bst4CI ACNGT 3 cut(s) 679, 905, 1015
Bst6I CTCTTC 2 cut(s) 1037, 1344
BstACI GRCGYC 2 cut(s) 8, 979
BstAPI GCANNNNNTGC 1 cut(s) 1326
BstAUI TGTACA 1 cut(s) 586
BstBAI YACGTR 2 cut(s) 619, 1009
BstC8I GCNNGC 2 cut(s) 34, 558
BstDEI CTNAG 4 cut(s) 851, 881, 1000, 1386
BstF5I GGATG 4 cut(s) 79, 602, 1211, 1419
BstFNI CGCG 1 cut(s) 578
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 3 cut(s) 32, 580, 688
BstKTI GATC 3 cut(s) 353, 360, 1059
BstMAI GTCTC 5 cut(s) 223, 281, 540, 961, 1029
BstMBI GATC 3 cut(s) 350, 357, 1056
BstMCI CGRYCG 2 cut(s) 83, 792
BstMWI GCNNNNNNNGC 8 cut(s) 29, 156, 311, 430, 455, 566, 575, 1326
BstNI CCWGG 1 cut(s) 806
BstNSI RCATGY 2 cut(s) 331, 346
BstSCI CCNGG 2 cut(s) 804, 1427
BstSLI GKGCMC 2 cut(s) 129, 327
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 4 cut(s) 925, 1001, 1110, 1123
BstV2I GAAGAC 1 cut(s) 394
BstZI CGGCCG 1 cut(s) 789
BsuRI GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
BtgZI GCGATG 2 cut(s) 115, 559
BtrI CACGTC 2 cut(s) 320, 445
BtsCI GGATG 4 cut(s) 79, 602, 1211, 1419
BtsI GCAGTG 3 cut(s) 11, 30, 165
BtsIMutI CAGTG 3 cut(s) 11, 30, 165
Cac8I GCNNGC 2 cut(s) 34, 558
CaiI CAGNNNCTG 2 cut(s) 1027, 1216
CfoI GCGC 3 cut(s) 32, 580, 688
Cfr10I RCCGGY 2 cut(s) 32, 55
Cfr13I GGNCC 6 cut(s) 125, 126, 312, 410, 1408, 1425
CpoI CGGWCCG 1 cut(s) 1408
CseI GACGC 3 cut(s) 16, 298, 1156
Csp6I GTAC 2 cut(s) 587, 1364
CspI CGGWCCG 1 cut(s) 1408
CviQI GTAC 2 cut(s) 587, 1364
DdeI CTNAG 4 cut(s) 851, 881, 1000, 1386
DpnI GATC 3 cut(s) 352, 359, 1058
DpnII GATC 3 cut(s) 350, 357, 1056
DraI TTTAAA 1 cut(s) 420
DraIII CACNNNGTG 1 cut(s) 25
DriI GACNNNNNGTC 1 cut(s) 1017
EaeI YGGCCR 4 cut(s) 187, 298, 789, 844
EagI CGGCCG 1 cut(s) 789
Eam1104I CTCTTC 2 cut(s) 1037, 1344
Eam1105I GACNNNNNGTC 1 cut(s) 1017
EarI CTCTTC 2 cut(s) 1037, 1344
EciI GGCGGA 3 cut(s) 163, 202, 1218
Ecl136II GAGCTC 1 cut(s) 208
EclXI CGGCCG 1 cut(s) 789
Eco24I GRGCYC 2 cut(s) 129, 210
Eco47I GGWCC 1 cut(s) 1408
Eco52I CGGCCG 1 cut(s) 789
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 804
EcoT38I GRGCYC 2 cut(s) 129, 210
Esp3I CGTCTC 2 cut(s) 223, 281
FaqI GGGAC 3 cut(s) 990, 1227, 1345
FauI CCCGC 2 cut(s) 29, 1322
FauNDI CATATG 1 cut(s) 758
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 4 cut(s) 939, 990, 1099, 1112
FokI GGATG 4 cut(s) 86, 609, 1218, 1426
FriOI GRGCYC 2 cut(s) 129, 210
Fsp4HI GCNGC 4 cut(s) 939, 990, 1099, 1112
FspBI CTAG 3 cut(s) 203, 471, 1130
FspI TGCGCA 1 cut(s) 687
GlaI GCGC 3 cut(s) 31, 579, 687
GluI GCNGC 4 cut(s) 939, 990, 1099, 1112
GsuI CTGGAG 1 cut(s) 827
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
HapII CCGG 8 cut(s) 33, 56, 315, 408, 459, 772, 1411, 1428
HgaI GACGC 3 cut(s) 16, 298, 1156
HhaI GCGC 3 cut(s) 32, 580, 688
Hin1I GRCGYC 2 cut(s) 8, 979
Hin6I GCGC 3 cut(s) 30, 578, 686
HinP1I GCGC 3 cut(s) 30, 578, 686
HindIII AAGCTT 2 cut(s) 303, 422
HinfI GANTC 6 cut(s) 785, 875, 954, 1038, 1051, 1156
HpaII CCGG 8 cut(s) 33, 56, 315, 408, 459, 772, 1411, 1428
HphI GGTGA 2 cut(s) 70, 130
Hpy166II GTNNAC 7 cut(s) 325, 340, 369, 589, 726, 1006, 1092
Hpy188I TCNGA 5 cut(s) 197, 1037, 1056, 1145, 1199
Hpy188III TCNNGA 2 cut(s) 370, 1060
Hpy8I GTNNAC 7 cut(s) 325, 340, 369, 589, 726, 1006, 1092
Hpy99I CGWCG 1 cut(s) 1448
HpyAV CCTTC 6 cut(s) 145, 256, 514, 539, 713, 1297
HpyCH4III ACNGT 3 cut(s) 679, 905, 1015
HpyCH4IV ACGT 5 cut(s) 319, 444, 618, 979, 1008
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 156, 311, 430, 455, 566, 575, 1326
HpyF3I CTNAG 4 cut(s) 851, 881, 1000, 1386
HpySE526I ACGT 5 cut(s) 319, 444, 618, 979, 1008
Hsp92I GRCGYC 2 cut(s) 8, 979
HspAI GCGC 3 cut(s) 30, 578, 686
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Kzo9I GATC 3 cut(s) 350, 357, 1056
LguI GCTCTTC 2 cut(s) 1037, 1344
LmnI GCTCC 1 cut(s) 63
Lsp1109I GCAGC 4 cut(s) 925, 1001, 1110, 1123
LweI GCATC 3 cut(s) 64, 786, 939
MaeI CTAG 3 cut(s) 203, 471, 1130
MaeII ACGT 5 cut(s) 319, 444, 618, 979, 1008
MaeIII GTNAC 3 cut(s) 838, 1009, 1147
MalI GATC 3 cut(s) 352, 359, 1058
MboI GATC 3 cut(s) 350, 357, 1056
MboII GAAGA 9 cut(s) 127, 394, 449, 1024, 1130, 1243, 1331, 1429, 1462
MhlI GDGCHC 3 cut(s) 129, 210, 327
MlsI TGGCCA 2 cut(s) 300, 846
MluCI AATT 4 cut(s) 895, 1106, 1291, 1308
MluNI TGGCCA 2 cut(s) 300, 846
MlyI GAGTC 2 cut(s) 794, 963
MmeI TCCRAC 2 cut(s) 984, 1195
MnlI CCTC 5 cut(s) 54, 395, 1193, 1310, 1384
Mox20I TGGCCA 2 cut(s) 300, 846
MreI CGCCGGCG 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 32
MscI TGGCCA 2 cut(s) 300, 846
MseI TTAA 3 cut(s) 419, 893, 1395
MslI CAYNNNNRTG 2 cut(s) 617, 1046
Msp20I TGGCCA 2 cut(s) 300, 846
MspI CCGG 8 cut(s) 33, 56, 315, 408, 459, 772, 1411, 1428
MspR9I CCNGG 2 cut(s) 806, 1429
Mva1269I GAATGC 2 cut(s) 569, 1114
MvaI CCWGG 1 cut(s) 806
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 8 cut(s) 29, 156, 311, 430, 455, 566, 575, 1326
NaeI GCCGGC 1 cut(s) 34
NciI CCSGG 1 cut(s) 1429
NdeI CATATG 1 cut(s) 758
NdeII GATC 3 cut(s) 350, 357, 1056
NgoMIV GCCGGC 1 cut(s) 32
NlaIV GGNNCC 5 cut(s) 41, 127, 160, 313, 1240
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 2 cut(s) 1009, 1147
NsbI TGCGCA 1 cut(s) 687
NspI RCATGY 2 cut(s) 331, 346
PciSI GCTCTTC 2 cut(s) 1037, 1344
PctI GAATGC 2 cut(s) 569, 1114
PdiI GCCGGC 1 cut(s) 34
PfeI GAWTC 4 cut(s) 875, 1038, 1051, 1156
PkrI GCNGC 4 cut(s) 940, 991, 1100, 1113
PleI GAGTC 2 cut(s) 793, 962
PpsI GAGTC 2 cut(s) 793, 962
Ppu21I YACGTR 2 cut(s) 619, 1009
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 804
PspGI CCWGG 1 cut(s) 804
PspN4I GGNNCC 5 cut(s) 41, 127, 160, 313, 1240
PspOMI GGGCCC 1 cut(s) 125
PspPI GGNCC 6 cut(s) 125, 126, 312, 410, 1408, 1425
PstNI CAGNNNCTG 2 cut(s) 1027, 1216
RsaI GTAC 2 cut(s) 588, 1365
RsaNI GTAC 2 cut(s) 587, 1364
RseI CAYNNNNRTG 2 cut(s) 617, 1046
Rsr2I CGGWCCG 1 cut(s) 1408
RsrII CGGWCCG 1 cut(s) 1408
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 2 cut(s) 1037, 1344
SaqAI TTAA 3 cut(s) 419, 893, 1395
SatI GCNGC 4 cut(s) 939, 990, 1099, 1112
Sau3AI GATC 3 cut(s) 350, 357, 1056
Sau96I GGNCC 6 cut(s) 125, 126, 312, 410, 1408, 1425
SchI GAGTC 2 cut(s) 794, 963
ScrFI CCNGG 2 cut(s) 806, 1429
SduI GDGCHC 3 cut(s) 129, 210, 327
SfaNI GCATC 3 cut(s) 64, 786, 939
SgrAI CRCCGGYG 1 cut(s) 32
SinI GGWCC 1 cut(s) 1408
SmiMI CAYNNNNRTG 2 cut(s) 617, 1046
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 4 cut(s) 895, 1106, 1291, 1308
SsiI CCGC 6 cut(s) 36, 174, 213, 485, 1203, 1329
SspI AATATT 1 cut(s) 694
SspMI CTAG 3 cut(s) 203, 471, 1130
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 2 cut(s) 804, 1427
TaaI ACNGT 3 cut(s) 679, 905, 1015
TaiI ACGT 5 cut(s) 322, 447, 621, 982, 1011
TaqI TCGA 2 cut(s) 624, 952
TaqII GACCGA 1 cut(s) 69
TasI AATT 4 cut(s) 895, 1106, 1291, 1308
TatI WGTACW 2 cut(s) 586, 1363
TfiI GAWTC 4 cut(s) 875, 1038, 1051, 1156
Tru1I TTAA 3 cut(s) 419, 893, 1395
Tru9I TTAA 3 cut(s) 419, 893, 1395
TscAI CASTG 3 cut(s) 18, 30, 172
TseFI GTSAC 2 cut(s) 1009, 1147
TseI GCWGC 4 cut(s) 938, 989, 1098, 1111
Tsp45I GTSAC 2 cut(s) 1009, 1147
TspDTI ATGAA 7 cut(s) 111, 250, 562, 601, 719, 913, 1430
TspGWI ACGGA 2 cut(s) 223, 1421
TspRI CASTG 3 cut(s) 18, 30, 172
VneI GTGCAC 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 1408
XapI RAATTY 2 cut(s) 895, 1308
XceI RCATGY 2 cut(s) 331, 346
XmiI GTMKAC 1 cut(s) 1005
XspI CTAG 3 cut(s) 203, 471, 1130
ZraI GACGTC 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.