pycom01g21650

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000970
Physical Location & Seq
Reverse (-)
19588570 .. 19591666
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21650.8

Sequence Viewer

Length: 1509 bp
ATGATCCTTGAGCAGCTGCTCGACCCAGCCACCCGCAGCACCACCGAGTTGAGCAATAAACAGCTTATGTACGAAGGTGAGAACGAAGACTATGCGCCGGCGAGGAGCTTTGGTTCGTTGCGGCGTATGTTTTGGATAGAGACGGTGAAGCTGTGGCAAATAGGCGGTTCTGCAGTTATCAATCTCATGTGCATGTATGGGGCTAACTCAGTTATCGTGCTCTTTGCCGGTCATGTCGGAACTATTGAGCTCTCCGCCATCTCCATTTCTCTCTCTGTCATATCCACATTCACTTATGGATTCATGCTTGGGATGGGGAGTGCTCTAGAGACCCTGTGTGGACAAGCATTTGGTGCTGGACAAATTCACATGCTTGGGATCTACATGCAACGATCATGTATAATTCTATTCGTAACCGCCTTCTTACTCTTGCCGATTTACATCTTCGCCACACCGATTCTAAAGTGGCTAGGCCAAGAAGACGACATAGCGAATGAAGCCGGAAAATTTACACTCCAAATAATCCCCCAATTGTTCTCACTTGCCATAAATTTCCCAGCTGAAAAGTTCCTCCAGGCTCAGAGGAAGGTGAAGGTGCTGGCATGGATTGCAGTGTTGGCCCTGGTCATACACATTGGAATGCTAACGCTCTTCATGTATGTGTTTGATTGGGGCACATTGGGGGCAGCTGTGTCATTTAACCTCACAAGATGGGGGATTTCAATTGCACAAGCTGTGTATATTATGGGTTGGTGCGAGGGTTGGACTGGATTTTCATGGCTGGCATTCAATGAGATTTGGGCATTTGTGAGACTTTCTCTTGCCTCTGCAGTCATGCTCTGCCTAGAAATTTGGTACATGATGAGTATGCTCATTCTCACGGGCGGTCTTTCTGATGCAGTTATAGCCGTTGGTTCTCTTTCAATTTGCATGAACGTCAACGGGTGGGAAACAATGTTGTTCGTTGGAATAAATGCTGCAATAAGTGTTCGTGTCTCCAATGAGCTTGGATTAAGACGTCCTAGAGCAGCCAAATACGCCGTTTATGTGACAATATCTCAATCTCTCTTGCTTGGGCTTCTTTTCATGATTATTATTTTAATAACCAAAGACGATTTTGCCGTAATTTTCACAAGTGACAAGGAGCTGCAACAAGCGGTTGCTAAGCTTGCATACCTTCTTGGTATAACTATGGTGCTCAACAGTGTCCAACCAGTAATATCAGGTGTTGCAATTGGAGGTGGATGGCAGGCAACGGTTGCTTATATAAACTTGGGTTGTTACTACATTTTTGGACTTCCTCTTGGATACGTTCTTTGCCATGTTGCAAATTTAGGAGTCACGGGACTTTGGGGGGGCATGATATGTGGAACTGCTCTCCAAACCATACTTCTGCTAATTATACTTTACAAAACAAACTGGAACAAAGAGGTGGAGCAAGCAACAAATCGTGTCCGGAACTGGGGAGGGCAAGACATCAAAACTGCCAACGGAGCTGAAAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

503

Amino Acids

55.15

Weight (kDa)

6.13

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1021
AccIII TCCGGA 1 cut(s) 1455
AciI CCGC 7 cut(s) 34, 121, 165, 255, 417, 885, 1157
AclWI GGATC 1 cut(s) 386
AcsI RAATTY 5 cut(s) 363, 506, 550, 849, 1330
AcyI GRCGYC 1 cut(s) 1018
AfaI GTAC 3 cut(s) 71, 857, 1504
AfiI CCNNNNNNNGG 1 cut(s) 1462
AgsI TTSAA 3 cut(s) 723, 790, 924
AjnI CCWGG 2 cut(s) 573, 621
AjuI GAANNNNNNNTTGG 2 cut(s) 1374, 1406
AloI GAACNNNNNNTCC 2 cut(s) 97, 129
Alw21I GWGCWC 4 cut(s) 222, 252, 325, 1200
Alw26I GTCTC 4 cut(s) 134, 323, 805, 1000
AlwI GGATC 1 cut(s) 386
Aor13HI TCCGGA 1 cut(s) 1455
AoxI GGCC 2 cut(s) 472, 618
ApeKI GCWGC 7 cut(s) 13, 16, 36, 686, 977, 1028, 1147
ApoI RAATTY 5 cut(s) 363, 506, 550, 849, 1330
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 1 cut(s) 619
AsuHPI GGTGA 3 cut(s) 89, 157, 601
BaeGI GKGCMC 1 cut(s) 677
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 2 cut(s) 93, 486
Bbv12I GWGCWC 4 cut(s) 222, 252, 325, 1200
BbvI GCAGC 7 cut(s) 3, 25, 48, 698, 964, 1040, 1134
BccI CCATC 4 cut(s) 266, 307, 705, 1239
BceAI ACGGC 3 cut(s) 893, 1025, 1106
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 2 cut(s) 575, 623
BciVI GTATCC 1 cut(s) 1301
BcoDI GTCTC 4 cut(s) 134, 323, 805, 1000
BfaI CTAG 4 cut(s) 326, 470, 845, 1023
BfmI CTRYAG 2 cut(s) 171, 828
BfuI GTATCC 1 cut(s) 1301
BisI GCNGC 8 cut(s) 14, 17, 37, 122, 687, 978, 1029, 1148
BlpI GCTNAGC 1 cut(s) 1164
BlsI GCNGC 8 cut(s) 15, 18, 38, 123, 688, 979, 1030, 1149
BmcAI AGTACT 1 cut(s) 1504
Bme1390I CCNGG 2 cut(s) 575, 623
BmgT120I GGNCC 1 cut(s) 619
BmrFI CCNGG 2 cut(s) 575, 623
BmrI ACTGGG 1 cut(s) 1471
BmsI GCATC 1 cut(s) 886
BmuI ACTGGG 1 cut(s) 1471
BpiI GAAGAC 2 cut(s) 93, 486
BplI GAGNNNNNCTC 2 cut(s) 802, 834
BpmI CTGGAG 1 cut(s) 557
Bpu1102I GCTNAGC 1 cut(s) 1164
BpuEI CTTGAG 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 1018
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 1 cut(s) 621
BsaWI WCCGGW 1 cut(s) 1455
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 1462
Bse118I RCCGGY 2 cut(s) 97, 227
Bse1I ACTGG 4 cut(s) 772, 1214, 1424, 1466
Bse8I GATNNNNATC 1 cut(s) 440
BseAI TCCGGA 1 cut(s) 1455
BseBI CCWGG 2 cut(s) 575, 623
BseDI CCNNGG 1 cut(s) 621
BseGI GGATG 2 cut(s) 318, 1250
BseJI GATNNNNATC 1 cut(s) 440
BseLI CCNNNNNNNGG 1 cut(s) 1462
BseMII CTCAG 2 cut(s) 222, 593
BseNI ACTGG 4 cut(s) 772, 1214, 1424, 1466
BseRI GAGGAG 1 cut(s) 118
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 7 cut(s) 3, 25, 48, 698, 964, 1040, 1134
BseYI CCCAGC 2 cut(s) 25, 556
BshFI GGCC 2 cut(s) 474, 620
BsiHKAI GWGCWC 4 cut(s) 222, 252, 325, 1200
BsiSI CCGG 4 cut(s) 98, 228, 501, 1456
BslFI GGGAC 1 cut(s) 1359
BslI CCNNNNNNNGG 1 cut(s) 1462
BsmAI GTCTC 4 cut(s) 134, 323, 805, 1000
BsmBI CGTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 1359
BsmI GAATGC 2 cut(s) 645, 785
BsnI GGCC 2 cut(s) 474, 620
Bso31I GGTCTC 1 cut(s) 323
Bsp1286I GDGCHC 5 cut(s) 222, 252, 325, 677, 1200
Bsp13I TCCGGA 1 cut(s) 1455
Bsp143I GATC 3 cut(s) 3, 378, 392
Bsp1720I GCTNAGC 1 cut(s) 1164
BspACI CCGC 7 cut(s) 34, 121, 165, 255, 417, 885, 1157
BspANI GGCC 2 cut(s) 474, 620
BspCNI CTCAG 2 cut(s) 221, 592
BspEI TCCGGA 1 cut(s) 1455
BspHI TCATGA 1 cut(s) 1086
BspMAI CTGCAG 2 cut(s) 175, 832
BspPI GGATC 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 323
BsrFI RCCGGY 2 cut(s) 97, 227
BsrI ACTGG 4 cut(s) 772, 1214, 1424, 1466
BssAI RCCGGY 2 cut(s) 97, 227
BssECI CCNNGG 1 cut(s) 621
BssMI GATC 3 cut(s) 3, 378, 392
BssNI GRCGYC 1 cut(s) 1018
Bst2UI CCWGG 2 cut(s) 575, 623
Bst4CI ACNGT 3 cut(s) 145, 1205, 1258
Bst6I CTCTTC 1 cut(s) 656
BstACI GRCGYC 1 cut(s) 1018
BstAPI GCANNNNNTGC 3 cut(s) 353, 608, 1259
BstC8I GCNNGC 6 cut(s) 99, 600, 783, 1170, 1251, 1440
BstDEI CTNAG 3 cut(s) 208, 579, 1164
BstF5I GGATG 2 cut(s) 318, 1250
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 3 cut(s) 6, 381, 395
BstMAI GTCTC 4 cut(s) 134, 323, 805, 1000
BstMBI GATC 3 cut(s) 3, 378, 392
BstMWI GCNNNNNNNGC 9 cut(s) 353, 497, 608, 617, 905, 1037, 1169, 1259, 1493
BstNI CCWGG 2 cut(s) 575, 623
BstNSI RCATGY 3 cut(s) 196, 373, 388
BstSCI CCNGG 2 cut(s) 573, 621
BstSFI CTRYAG 2 cut(s) 171, 828
BstSLI GKGCMC 1 cut(s) 677
BstV1I GCAGC 7 cut(s) 3, 25, 48, 698, 964, 1040, 1134
BstV2I GAAGAC 2 cut(s) 93, 486
BstX2I RGATCY 1 cut(s) 378
BstYI RGATCY 1 cut(s) 378
BsuI GTATCC 1 cut(s) 1301
BsuRI GGCC 2 cut(s) 474, 620
BtsCI GGATG 2 cut(s) 318, 1250
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 2 cut(s) 618, 1210
Cac8I GCNNGC 6 cut(s) 99, 600, 783, 1170, 1251, 1440
CciI TCATGA 1 cut(s) 1086
CfoI GCGC 1 cut(s) 97
Cfr10I RCCGGY 2 cut(s) 97, 227
Cfr13I GGNCC 1 cut(s) 619
Csp6I GTAC 3 cut(s) 70, 856, 1503
CviQI GTAC 3 cut(s) 70, 856, 1503
DdeI CTNAG 3 cut(s) 208, 579, 1164
DpnI GATC 3 cut(s) 5, 380, 394
DpnII GATC 3 cut(s) 3, 378, 392
Eam1104I CTCTTC 1 cut(s) 656
EarI CTCTTC 1 cut(s) 656
EciI GGCGGA 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 573, 621
EcoT38I GRGCYC 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 134
FaqI GGGAC 1 cut(s) 1359
FauI CCCGC 1 cut(s) 41
Fnu4HI GCNGC 8 cut(s) 14, 17, 37, 122, 687, 978, 1029, 1148
FokI GGATG 2 cut(s) 325, 1257
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 8 cut(s) 14, 17, 37, 122, 687, 978, 1029, 1148
FspBI CTAG 4 cut(s) 326, 470, 845, 1023
GlaI GCGC 1 cut(s) 96
GluI GCNGC 8 cut(s) 14, 17, 37, 122, 687, 978, 1029, 1148
GsaI CCCAGC 2 cut(s) 29, 560
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 2 cut(s) 474, 620
HapII CCGG 4 cut(s) 98, 228, 501, 1456
HhaI GCGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 1018
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 940
HindII GTYRAC 1 cut(s) 940
HindIII AAGCTT 1 cut(s) 1166
HinfI GANTC 3 cut(s) 300, 457, 1338
HpaII CCGG 4 cut(s) 98, 228, 501, 1456
HphI GGTGA 3 cut(s) 89, 157, 601
Hpy166II GTNNAC 2 cut(s) 341, 940
Hpy188I TCNGA 3 cut(s) 239, 582, 895
Hpy188III TCNNGA 3 cut(s) 326, 1087, 1456
Hpy8I GTNNAC 2 cut(s) 341, 940
HpyAV CCTTC 5 cut(s) 68, 430, 580, 586, 1187
HpyCH4III ACNGT 3 cut(s) 145, 1205, 1258
HpyCH4IV ACGT 3 cut(s) 936, 1018, 1311
HpyF10VI GCNNNNNNNGC 9 cut(s) 353, 497, 608, 617, 905, 1037, 1169, 1259, 1493
HpyF3I CTNAG 3 cut(s) 208, 579, 1164
HpySE526I ACGT 3 cut(s) 936, 1018, 1311
Hsp92I GRCGYC 1 cut(s) 1018
HspAI GCGC 1 cut(s) 95
Kpn2I TCCGGA 1 cut(s) 1455
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
Kzo9I GATC 3 cut(s) 3, 378, 392
LguI GCTCTTC 1 cut(s) 656
LmnI GCTCC 4 cut(s) 105, 1144, 1435, 1493
Lsp1109I GCAGC 7 cut(s) 3, 25, 48, 698, 964, 1040, 1134
LweI GCATC 1 cut(s) 886
MaeI CTAG 4 cut(s) 326, 470, 845, 1023
MaeII ACGT 3 cut(s) 936, 1018, 1311
MaeIII GTNAC 5 cut(s) 412, 1048, 1136, 1280, 1339
MalI GATC 3 cut(s) 5, 380, 394
MboI GATC 3 cut(s) 3, 378, 392
MboII GAAGA 4 cut(s) 98, 436, 491, 643
MfeI CAATTG 3 cut(s) 530, 723, 1233
MflI RGATCY 1 cut(s) 378
MhlI GDGCHC 5 cut(s) 222, 252, 325, 677, 1200
MlyI GAGTC 1 cut(s) 1347
MmeI TCCRAC 4 cut(s) 217, 743, 946, 1234
MreI CGCCGGCG 1 cut(s) 97
MroI TCCGGA 1 cut(s) 1455
MroNI GCCGGC 1 cut(s) 97
MseI TTAA 3 cut(s) 699, 1013, 1100
MslI CAYNNNNRTG 3 cut(s) 191, 638, 659
MspA1I CMGCKG 3 cut(s) 16, 560, 689
MspI CCGG 4 cut(s) 98, 228, 501, 1456
MspR9I CCNGG 2 cut(s) 575, 623
MunI CAATTG 3 cut(s) 530, 723, 1233
Mva1269I GAATGC 2 cut(s) 645, 785
MvaI CCWGG 2 cut(s) 575, 623
MwoI GCNNNNNNNGC 9 cut(s) 353, 497, 608, 617, 905, 1037, 1169, 1259, 1493
NaeI GCCGGC 1 cut(s) 99
NdeII GATC 3 cut(s) 3, 378, 392
NgoMIV GCCGGC 1 cut(s) 97
NmuCI GTSAC 3 cut(s) 1048, 1136, 1339
NspI RCATGY 3 cut(s) 196, 373, 388
PagI TCATGA 1 cut(s) 1086
PciSI GCTCTTC 1 cut(s) 656
PcsI WCGNNNNNNNCGW 1 cut(s) 1119
PctI GAATGC 2 cut(s) 645, 785
PdiI GCCGGC 1 cut(s) 99
PfeI GAWTC 2 cut(s) 300, 457
PkrI GCNGC 8 cut(s) 15, 18, 38, 123, 688, 979, 1030, 1149
PleI GAGTC 1 cut(s) 1346
PpsI GAGTC 1 cut(s) 1346
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 573, 621
PspFI CCCAGC 2 cut(s) 25, 556
PspGI CCWGG 2 cut(s) 573, 621
PspPI GGNCC 1 cut(s) 619
PstI CTGCAG 2 cut(s) 175, 832
PsuI RGATCY 1 cut(s) 378
PvuII CAGCTG 3 cut(s) 16, 560, 689
RsaI GTAC 3 cut(s) 71, 857, 1504
RsaNI GTAC 3 cut(s) 70, 856, 1503
RseI CAYNNNNRTG 3 cut(s) 191, 638, 659
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 656
SaqAI TTAA 3 cut(s) 699, 1013, 1100
SatI GCNGC 8 cut(s) 14, 17, 37, 122, 687, 978, 1029, 1148
Sau3AI GATC 3 cut(s) 3, 378, 392
Sau96I GGNCC 1 cut(s) 619
ScaI AGTACT 1 cut(s) 1504
SchI GAGTC 1 cut(s) 1347
ScrFI CCNGG 2 cut(s) 575, 623
SduI GDGCHC 5 cut(s) 222, 252, 325, 677, 1200
SfaNI GCATC 1 cut(s) 886
SfcI CTRYAG 2 cut(s) 171, 828
SgrAI CRCCGGYG 1 cut(s) 97
SmiMI CAYNNNNRTG 3 cut(s) 191, 638, 659
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
SsiI CCGC 7 cut(s) 34, 121, 165, 255, 417, 885, 1157
SspMI CTAG 4 cut(s) 326, 470, 845, 1023
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 573, 621
TaaI ACNGT 3 cut(s) 145, 1205, 1258
TaiI ACGT 3 cut(s) 939, 1021, 1314
TaqI TCGA 1 cut(s) 21
TatI WGTACW 1 cut(s) 1502
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 2 cut(s) 300, 457
Tru1I TTAA 3 cut(s) 699, 1013, 1100
Tru9I TTAA 3 cut(s) 699, 1013, 1100
TscAI CASTG 2 cut(s) 618, 1210
TseFI GTSAC 3 cut(s) 1048, 1136, 1339
TseI GCWGC 7 cut(s) 13, 16, 36, 686, 977, 1028, 1147
Tsp45I GTSAC 3 cut(s) 1048, 1136, 1339
TspDTI ATGAA 6 cut(s) 292, 510, 643, 765, 947, 1075
TspGWI ACGGA 1 cut(s) 1506
TspRI CASTG 2 cut(s) 618, 1210
XapI RAATTY 5 cut(s) 363, 506, 550, 849, 1330
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 3 cut(s) 196, 373, 388
XspI CTAG 4 cut(s) 326, 470, 845, 1023
ZraI GACGTC 1 cut(s) 1019
ZrmI AGTACT 1 cut(s) 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.