pycom07g25230

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000970
Physical Location & Seq
Reverse (-)
25621056 .. 25622035
980 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25230.2

Sequence Viewer

Length: 513 bp
ATGGAGGAGCCGCTGCTCGACACAGCTGCCGGCAGCGCTGCCGAGTTGAGCAGTAAGCATCCTATGTACGAAGGTGAGACTGAAGACTACGCGCCCGTGAGGAGCTTTGATGCGCTCCGGCGTATTTTTTGGATAGAGACGGTGAAGATGTGCTTGATGGGGAGTGCTTTGGAGACCCTGTGTGGACAAGCATTTGGTGCTGGACAAATTCCCATGCTTGGGATCTACATGCAACGCTCATGGATAATCTTATTCGTAACCTGCTTCTTACTCTTGCCGATTTACATCTTCGCTACACCGATTCTGAGGTGGCTAGGCCAAGAAGACGACATAGTGATTGAAGCCGGAAAGTATACCCTCCAAATAATCCCCCAATTATTCTCACTTTCCTTAACTTTCCCAGCTCAGAAGTTCCTCCAGGCTCAGACGAAGGTGAAGGTGCTGGCATGGATTGCAGTCTTGGCCCTTGGCCATGCACGATGGAATGCTAGCGCTCTTCATGTATGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

171

Amino Acids

19.01

Weight (kDa)

5.56

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 269
AccI GTMKAC 1 cut(s) 353
AccII CGCG 1 cut(s) 92
AciI CCGC 1 cut(s) 11
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 1 cut(s) 469
AcsI RAATTY 1 cut(s) 207
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 1 cut(s) 68
AfeI AGCGCT 2 cut(s) 37, 493
AfiI CCNNNNNNNGG 2 cut(s) 218, 219
AgsI TTSAA 1 cut(s) 341
AjnI CCWGG 1 cut(s) 417
AluBI AGCT 3 cut(s) 26, 105, 404
AluI AGCT 3 cut(s) 26, 105, 404
Alw26I GTCTC 3 cut(s) 71, 131, 167
AlwI GGATC 1 cut(s) 230
Aor51HI AGCGCT 2 cut(s) 37, 493
AoxI GGCC 3 cut(s) 316, 462, 469
ApeKI GCWGC 4 cut(s) 13, 26, 33, 38
ApoI RAATTY 1 cut(s) 207
AspLEI GCGC 4 cut(s) 38, 94, 115, 494
AspS9I GGNCC 1 cut(s) 463
AsuHPI GGTGA 3 cut(s) 86, 154, 445
AsuNHI GCTAGC 1 cut(s) 488
BalI TGGCCA 1 cut(s) 471
BbsI GAAGAC 2 cut(s) 90, 330
BbvI GCAGC 3 cut(s) 13, 25, 45
BccI CCATC 2 cut(s) 151, 474
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BciT130I CCWGG 1 cut(s) 419
BcoDI GTCTC 3 cut(s) 71, 131, 167
BfaI CTAG 2 cut(s) 314, 489
BfoI RGCGCY 2 cut(s) 39, 495
BfuAI ACCTGC 1 cut(s) 269
BisI GCNGC 5 cut(s) 11, 14, 27, 34, 39
BlsI GCNGC 5 cut(s) 12, 15, 28, 35, 40
Bme1390I CCNGG 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 2 cut(s) 67, 100
BmtI GCTAGC 1 cut(s) 492
BpiI GAAGAC 2 cut(s) 90, 330
BpmI CTGGAG 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 284
BsaI GGTCTC 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 466
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 219
Bse118I RCCGGY 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 284
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 1 cut(s) 466
BseGI GGATG 1 cut(s) 58
BseJI GATNNNNATC 1 cut(s) 284
BseLI CCNNNNNNNGG 2 cut(s) 218, 219
BseMII CTCAG 3 cut(s) 296, 419, 437
BseRI GAGGAG 2 cut(s) 20, 115
BseXI GCAGC 3 cut(s) 13, 25, 45
BseYI CCCAGC 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 3 cut(s) 318, 464, 471
BsiSI CCGG 3 cut(s) 30, 118, 345
BslI CCNNNNNNNGG 2 cut(s) 218, 219
BsmAI GTCTC 3 cut(s) 71, 131, 167
BsmBI CGTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 3 cut(s) 318, 464, 471
Bso31I GGTCTC 1 cut(s) 167
Bsp143I GATC 1 cut(s) 222
BspACI CCGC 1 cut(s) 11
BspANI GGCC 3 cut(s) 318, 464, 471
BspCNI CTCAG 3 cut(s) 297, 418, 436
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 9
BspMI ACCTGC 1 cut(s) 269
BspOI GCTAGC 1 cut(s) 492
BspPI GGATC 1 cut(s) 230
BspQI GCTCTTC 1 cut(s) 501
BspTNI GGTCTC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 1 cut(s) 222
BssNAI GTATAC 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 466
Bst1107I GTATAC 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 501
BstAPI GCANNNNNTGC 2 cut(s) 197, 452
BstC8I GCNNGC 3 cut(s) 31, 444, 490
BstDEI CTNAG 3 cut(s) 305, 405, 423
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 2 cut(s) 39, 495
BstHHI GCGC 4 cut(s) 38, 94, 115, 494
BstKTI GATC 1 cut(s) 225
BstMAI GTCTC 3 cut(s) 71, 131, 167
BstMBI GATC 1 cut(s) 222
BstMWI GCNNNNNNNGC 4 cut(s) 35, 197, 452, 461
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 1 cut(s) 417
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 3 cut(s) 13, 25, 45
BstV2I GAAGAC 2 cut(s) 90, 330
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BstZ17I GTATAC 1 cut(s) 354
BsuRI GGCC 3 cut(s) 318, 464, 471
BtsCI GGATG 1 cut(s) 58
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 31, 444, 490
CfoI GCGC 4 cut(s) 38, 94, 115, 494
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 463
Csp6I GTAC 1 cut(s) 67
CviAII CATG 6 cut(s) 214, 229, 240, 447, 473, 500
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 3 cut(s) 305, 405, 423
DpnI GATC 1 cut(s) 224
DpnII GATC 1 cut(s) 222
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 466
Eco31I GGTCTC 1 cut(s) 167
Eco47III AGCGCT 2 cut(s) 37, 493
Eco57I CTGAAG 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 466
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 6 cut(s) 217, 232, 243, 450, 476, 503
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 6 cut(s) 213, 228, 239, 446, 472, 499
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 5 cut(s) 11, 14, 27, 34, 39
FokI GGATG 1 cut(s) 45
Fsp4HI GCNGC 5 cut(s) 11, 14, 27, 34, 39
FspBI CTAG 2 cut(s) 314, 489
GlaI GCGC 4 cut(s) 37, 93, 114, 493
GluI GCNGC 5 cut(s) 11, 14, 27, 34, 39
GsaI CCCAGC 1 cut(s) 404
GsuI CTGGAG 1 cut(s) 401
HaeII RGCGCY 2 cut(s) 39, 495
HaeIII GGCC 3 cut(s) 318, 464, 471
HapII CCGG 3 cut(s) 30, 118, 345
HhaI GCGC 4 cut(s) 38, 94, 115, 494
Hin1II CATG 6 cut(s) 217, 232, 243, 450, 476, 503
Hin6I GCGC 4 cut(s) 36, 92, 113, 492
HinP1I GCGC 4 cut(s) 36, 92, 113, 492
HinfI GANTC 1 cut(s) 301
HpaII CCGG 3 cut(s) 30, 118, 345
HphI GGTGA 3 cut(s) 86, 154, 445
Hpy166II GTNNAC 2 cut(s) 185, 354
Hpy188I TCNGA 3 cut(s) 306, 408, 426
Hpy8I GTNNAC 2 cut(s) 185, 354
HpyAV CCTTC 3 cut(s) 65, 424, 430
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 3 cut(s) 232, 455, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 197, 452, 461
HpyF3I CTNAG 3 cut(s) 305, 405, 423
Hsp92II CATG 6 cut(s) 217, 232, 243, 450, 476, 503
HspAI GCGC 4 cut(s) 36, 92, 113, 492
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 222
LguI GCTCTTC 1 cut(s) 501
LmnI GCTCC 3 cut(s) 7, 102, 120
Lsp1109I GCAGC 3 cut(s) 13, 25, 45
LweI GCATC 2 cut(s) 67, 100
MaeI CTAG 2 cut(s) 314, 489
MaeIII GTNAC 1 cut(s) 256
MalI GATC 1 cut(s) 224
MboI GATC 1 cut(s) 222
MboII GAAGA 5 cut(s) 95, 157, 280, 335, 488
MflI RGATCY 1 cut(s) 222
MlsI TGGCCA 1 cut(s) 471
MluCI AATT 2 cut(s) 207, 374
MluNI TGGCCA 1 cut(s) 471
MnlI CCTC 4 cut(s) 93, 300, 368, 425
Mox20I TGGCCA 1 cut(s) 471
MroNI GCCGGC 1 cut(s) 29
MscI TGGCCA 1 cut(s) 471
MseI TTAA 1 cut(s) 392
MslI CAYNNNNRTG 1 cut(s) 504
Msp20I TGGCCA 1 cut(s) 471
MspA1I CMGCKG 2 cut(s) 13, 26
MspI CCGG 3 cut(s) 30, 118, 345
MspR9I CCNGG 1 cut(s) 419
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 35, 197, 452, 461
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 1 cut(s) 222
NgoMIV GCCGGC 1 cut(s) 29
NheI GCTAGC 1 cut(s) 488
NlaIII CATG 6 cut(s) 217, 232, 243, 450, 476, 503
NlaIV GGNNCC 1 cut(s) 9
NmeAIII GCCGAG 1 cut(s) 67
NspI RCATGY 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 501
PctI GAATGC 1 cut(s) 490
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 301
PkrI GCNGC 5 cut(s) 12, 15, 28, 35, 40
Psp6I CCWGG 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 400
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 463
PsuI RGATCY 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
RseI CAYNNNNRTG 1 cut(s) 504
SapI GCTCTTC 1 cut(s) 501
SaqAI TTAA 1 cut(s) 392
SatI GCNGC 5 cut(s) 11, 14, 27, 34, 39
Sau3AI GATC 1 cut(s) 222
Sau96I GGNCC 1 cut(s) 463
ScrFI CCNGG 1 cut(s) 419
SetI ASST 8 cut(s) 28, 76, 107, 263, 311, 406, 435, 441
SfaNI GCATC 2 cut(s) 67, 100
SmiMI CAYNNNNRTG 1 cut(s) 504
Sse9I AATT 2 cut(s) 207, 374
SsiI CCGC 1 cut(s) 11
SspMI CTAG 2 cut(s) 314, 489
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 18
TasI AATT 2 cut(s) 207, 374
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 1 cut(s) 301
Tru1I TTAA 1 cut(s) 392
Tru9I TTAA 1 cut(s) 392
TseI GCWGC 4 cut(s) 13, 26, 33, 38
TspDTI ATGAA 1 cut(s) 488
XapI RAATTY 1 cut(s) 207
XceI RCATGY 1 cut(s) 232
XmiI GTMKAC 1 cut(s) 353
XspI CTAG 2 cut(s) 314, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.