MD07G1277300.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000970
Physical Location & Seq
Reverse (-)
34256146 .. 34258420
2275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1277300.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGAGGAGCCGCTACTCGACACAGCTGCCGGCAGCACGGCCGAGTTGAGCAGTAAGCATCCTATGTACGAAGGTGAGACTGAAGACTATGCGCCCGTGAGGAGCTTTGATGCGCTCTGGCGTATGTTTTGGATAGAGACGGTGAAGTTGTGGCAAATAGGAGGTTCGGCAGTTATCACTATCATGTGTATGTATGGGACTAACGCAGTCATCATGCTCTTTTCCGGACATCTCGGAACTGTTGTGCTTTCTGCCATCTTTTCTCTAGCCGTCATTTCCACCTTCACATATGGGTTCATGCTTGGGATGGGGAGTGCTTTGGAGACCCTGTGTGGACAAGCATTTGGTGCTGGACAAATTCACATGCTTGGGATCTACATGCAACGCTCATGGATAATCTTATTCGTAACCTGCTTCTTACTCATGCCGCCGATTCTGAAGTGGCTAGGCCAAGAAGACGACATAGCGAATGAAGCCGGAAAGAATGTGAAGGTGCTGGCACGGATTGCAGTCTTGGCCCCGGTCATGCACATTGGAATGCTAGCGCTCTTCATGTATGTGTTTGATTGCGGCGCATTGGAGGCAGTTGTGTCATTTAACCTCACAAGATGGGGGATTGTAATTGCACAGGTTGCGTATATTACGGGTTGGTGCAAAGAGGGTTGGACTGGATTTTCATGGCTTGCATTCAATGAGATTTGGGCATTTGTCAGACTTTCTCTTGCCTCAGCTGTCATGCTATGCTTGGAAATTTGGTACTTGATGAGTCTTCTCATTCTCACAAGCAGTCTTTCTGATGCAGTTATAGCCCTTGGATCAAGATGCCCTAGAGCAGCCAAATATGTTGTTTATGTGACAGTATTTCAATATCTCTTAATTGGGATTCTTTTCATGATTATTATTTTAATAACCAAAGATGATATTACCTTACTTTTCACAAGTGACAAGGAGCTGCGACAAGCGGTCACTAAGCTTGCATGTTCTTGGTATAACTATGGTGCTCAAGAGTGTGTTGCAATTGGAGGTGGATGGCAGGGAATGGTAGCTTATATAAACTTTGGTTGCTACTACCTTTTTGAACTTCTTCTTGGATACGTTCTTTGCCATGTTGCAAATTTGGGAGTCATGGGACTGAGGATGATATGTGGAATTACTCTCCAAACCATACTTCTGCTAATCATACTTTACAAAACTAATTGGAACAAAGAGGTAATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

407

Amino Acids

45.2

Weight (kDa)

5.43

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 58 - 195 1.2e-15 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 419
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 4 cut(s) 11, 428, 570, 962
AclWI GGATC 2 cut(s) 380, 823
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 3 cut(s) 357, 750, 1114
AcuI CTGAAG 2 cut(s) 102, 458
AfaI GTAC 2 cut(s) 68, 758
AfeI AGCGCT 1 cut(s) 546
AgsI TTSAA 3 cut(s) 691, 866, 1079
AhdI GACNNNNNGTC 1 cut(s) 962
AjuI GAANNNNNNNTTGG 4 cut(s) 1071, 1103, 1152, 1184
AluBI AGCT 6 cut(s) 26, 105, 731, 952, 973, 1046
AluI AGCT 6 cut(s) 26, 105, 731, 952, 973, 1046
Alw21I GWGCWC 1 cut(s) 1003
Alw26I GTCTC 3 cut(s) 71, 131, 317
AlwI GGATC 2 cut(s) 380, 823
Aor13HI TCCGGA 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 546
AoxI GGCC 3 cut(s) 39, 448, 516
ApeKI GCWGC 4 cut(s) 26, 33, 833, 952
ApoI RAATTY 3 cut(s) 357, 750, 1114
AseI ATTAAT 1 cut(s) 1215
Asp700I GAANNNNTTC 1 cut(s) 1083
AspLEI GCGC 4 cut(s) 94, 115, 547, 575
AspS9I GGNCC 1 cut(s) 517
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 2 cut(s) 86, 154
AsuNHI GCTAGC 1 cut(s) 541
BbsI GAAGAC 3 cut(s) 90, 462, 761
Bbv12I GWGCWC 1 cut(s) 1003
BbvCI CCTCAGC 1 cut(s) 727
BbvI GCAGC 4 cut(s) 13, 45, 845, 939
BccI CCATC 4 cut(s) 263, 301, 603, 1023
BceAI ACGGC 2 cut(s) 54, 254
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BciVI GTATCC 1 cut(s) 1085
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 3 cut(s) 71, 131, 317
BfaI CTAG 4 cut(s) 266, 446, 542, 828
BfoI RGCGCY 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 419
BfuI GTATCC 1 cut(s) 1085
BisI GCNGC 7 cut(s) 11, 27, 34, 428, 571, 834, 953
BlsI GCNGC 7 cut(s) 12, 28, 35, 429, 572, 835, 954
Bme1390I CCNGG 1 cut(s) 521
BmeRI GACNNNNNGTC 1 cut(s) 962
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 2 cut(s) 9, 519
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 4 cut(s) 67, 100, 787, 812
BmtI GCTAGC 1 cut(s) 545
BpiI GAAGAC 3 cut(s) 90, 462, 761
Bpu10I CCTNAGC 1 cut(s) 727
BpuEI CTTGAG 1 cut(s) 987
BpuMI CCSGG 1 cut(s) 521
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 519, 811
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 673
BseAI TCCGGA 1 cut(s) 224
BseDI CCNNGG 2 cut(s) 519, 811
BseGI GGATG 4 cut(s) 58, 312, 1034, 1143
BseMII CTCAG 2 cut(s) 741, 1124
BseNI ACTGG 1 cut(s) 673
BseRI GAGGAG 2 cut(s) 20, 115
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 4 cut(s) 13, 45, 845, 939
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 3 cut(s) 41, 450, 518
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 1003
BsiSI CCGG 4 cut(s) 30, 225, 477, 521
BslFI GGGAC 2 cut(s) 211, 1143
BsmAI GTCTC 3 cut(s) 71, 131, 317
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 2 cut(s) 211, 1143
BsmI GAATGC 2 cut(s) 543, 686
BsnI GGCC 3 cut(s) 41, 450, 518
Bso31I GGTCTC 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 1003
Bsp13I TCCGGA 1 cut(s) 224
Bsp143I GATC 2 cut(s) 372, 815
BspACI CCGC 4 cut(s) 11, 428, 570, 962
BspANI GGCC 3 cut(s) 41, 450, 518
BspCNI CTCAG 2 cut(s) 740, 1125
BspEI TCCGGA 1 cut(s) 224
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 2 cut(s) 9, 519
BspMI ACCTGC 1 cut(s) 419
BspOI GCTAGC 1 cut(s) 545
BspPI GGATC 2 cut(s) 380, 823
BspQI GCTCTTC 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 1 cut(s) 673
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 2 cut(s) 519, 811
BssMI GATC 2 cut(s) 372, 815
BssT1I CCWWGG 1 cut(s) 811
Bst4CI ACNGT 3 cut(s) 142, 241, 859
Bst6I CTCTTC 1 cut(s) 554
BstAPI GCANNNNNTGC 3 cut(s) 347, 506, 632
BstC8I GCNNGC 5 cut(s) 31, 498, 543, 684, 975
BstDEI CTNAG 3 cut(s) 727, 969, 1133
BstF5I GGATG 4 cut(s) 58, 312, 1034, 1143
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 4 cut(s) 94, 115, 547, 575
BstKTI GATC 2 cut(s) 375, 818
BstMAI GTCTC 3 cut(s) 71, 131, 317
BstMBI GATC 2 cut(s) 372, 815
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 7 cut(s) 347, 473, 506, 515, 581, 632, 806
BstNSI RCATGY 3 cut(s) 367, 382, 981
BstSCI CCNGG 1 cut(s) 519
BstV1I GCAGC 4 cut(s) 13, 45, 845, 939
BstV2I GAAGAC 3 cut(s) 90, 462, 761
BstX2I RGATCY 1 cut(s) 372
BstYI RGATCY 1 cut(s) 372
BstZI CGGCCG 1 cut(s) 39
BsuI GTATCC 1 cut(s) 1085
BsuRI GGCC 3 cut(s) 41, 450, 518
BtsCI GGATG 4 cut(s) 58, 312, 1034, 1143
BveI ACCTGC 1 cut(s) 419
Cac8I GCNNGC 5 cut(s) 31, 498, 543, 684, 975
CciI TCATGA 1 cut(s) 891
CfoI GCGC 4 cut(s) 94, 115, 547, 575
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 2 cut(s) 67, 757
CviQI GTAC 2 cut(s) 67, 757
DdeI CTNAG 3 cut(s) 727, 969, 1133
DpnI GATC 2 cut(s) 374, 817
DpnII GATC 2 cut(s) 372, 815
DriI GACNNNNNGTC 1 cut(s) 962
EaeI YGGCCR 1 cut(s) 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 554
Eam1105I GACNNNNNGTC 1 cut(s) 962
EarI CTCTTC 1 cut(s) 554
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 811
Eco31I GGTCTC 1 cut(s) 317
Eco47III AGCGCT 1 cut(s) 546
Eco52I CGGCCG 1 cut(s) 39
Eco57I CTGAAG 2 cut(s) 102, 458
EcoT14I CCWWGG 1 cut(s) 811
ErhI CCWWGG 1 cut(s) 811
Esp3I CGTCTC 1 cut(s) 131
FaqI GGGAC 2 cut(s) 211, 1143
FauNDI CATATG 1 cut(s) 289
Fnu4HI GCNGC 7 cut(s) 11, 27, 34, 428, 571, 834, 953
FokI GGATG 4 cut(s) 45, 319, 1041, 1150
Fsp4HI GCNGC 7 cut(s) 11, 27, 34, 428, 571, 834, 953
FspBI CTAG 4 cut(s) 266, 446, 542, 828
GlaI GCGC 4 cut(s) 93, 114, 546, 574
GluI GCNGC 7 cut(s) 11, 27, 34, 428, 571, 834, 953
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 3 cut(s) 41, 450, 518
HapII CCGG 4 cut(s) 30, 225, 477, 521
HhaI GCGC 4 cut(s) 94, 115, 547, 575
Hin6I GCGC 4 cut(s) 92, 113, 545, 573
HinP1I GCGC 4 cut(s) 92, 113, 545, 573
HindIII AAGCTT 1 cut(s) 971
HinfI GANTC 4 cut(s) 433, 766, 883, 1122
HpaII CCGG 4 cut(s) 30, 225, 477, 521
HphI GGTGA 2 cut(s) 86, 154
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 4 cut(s) 236, 438, 713, 796
Hpy188III TCNNGA 4 cut(s) 225, 819, 892, 1004
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 3 cut(s) 65, 292, 484
HpyCH4III ACNGT 3 cut(s) 142, 241, 859
HpyCH4IV ACGT 1 cut(s) 1095
HpyF10VI GCNNNNNNNGC 7 cut(s) 347, 473, 506, 515, 581, 632, 806
HpyF3I CTNAG 3 cut(s) 727, 969, 1133
HpySE526I ACGT 1 cut(s) 1095
HspAI GCGC 4 cut(s) 92, 113, 545, 573
Kpn2I TCCGGA 1 cut(s) 224
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 2 cut(s) 372, 815
LguI GCTCTTC 1 cut(s) 554
LmnI GCTCC 3 cut(s) 7, 102, 949
Lsp1109I GCAGC 4 cut(s) 13, 45, 845, 939
LweI GCATC 4 cut(s) 67, 100, 787, 812
MaeI CTAG 4 cut(s) 266, 446, 542, 828
MaeII ACGT 1 cut(s) 1095
MaeIII GTNAC 4 cut(s) 406, 853, 941, 964
MalI GATC 2 cut(s) 374, 817
MboI GATC 2 cut(s) 372, 815
MboII GAAGA 5 cut(s) 95, 467, 541, 761, 1076
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 1 cut(s) 372
MhlI GDGCHC 1 cut(s) 1003
MlyI GAGTC 2 cut(s) 775, 1131
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 9 cut(s) 93, 155, 574, 611, 652, 736, 1016, 1128, 1201
MroI TCCGGA 1 cut(s) 224
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 1083
MseI TTAA 4 cut(s) 597, 875, 905, 1215
MslI CAYNNNNRTG 4 cut(s) 182, 188, 536, 557
MspA1I CMGCKG 2 cut(s) 26, 731
MspI CCGG 4 cut(s) 30, 225, 477, 521
MspR9I CCNGG 1 cut(s) 521
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 2 cut(s) 543, 686
MwoI GCNNNNNNNGC 7 cut(s) 347, 473, 506, 515, 581, 632, 806
NaeI GCCGGC 1 cut(s) 31
NciI CCSGG 1 cut(s) 521
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 372, 815
NgoMIV GCCGGC 1 cut(s) 29
NheI GCTAGC 1 cut(s) 541
NlaIV GGNNCC 2 cut(s) 9, 519
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 3 cut(s) 853, 941, 964
NspI RCATGY 3 cut(s) 367, 382, 981
PagI TCATGA 1 cut(s) 891
PciSI GCTCTTC 1 cut(s) 554
PctI GAATGC 2 cut(s) 543, 686
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 1083
PfeI GAWTC 2 cut(s) 433, 883
PkrI GCNGC 7 cut(s) 12, 28, 35, 429, 572, 835, 954
PleI GAGTC 2 cut(s) 774, 1130
PpsI GAGTC 2 cut(s) 774, 1130
PshBI ATTAAT 1 cut(s) 1215
PspN4I GGNNCC 2 cut(s) 9, 519
PspPI GGNCC 1 cut(s) 517
PsuI RGATCY 1 cut(s) 372
PvuII CAGCTG 2 cut(s) 26, 731
RsaI GTAC 2 cut(s) 68, 758
RsaNI GTAC 2 cut(s) 67, 757
RseI CAYNNNNRTG 4 cut(s) 182, 188, 536, 557
SapI GCTCTTC 1 cut(s) 554
SaqAI TTAA 4 cut(s) 597, 875, 905, 1215
SatI GCNGC 7 cut(s) 11, 27, 34, 428, 571, 834, 953
Sau3AI GATC 2 cut(s) 372, 815
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 2 cut(s) 775, 1131
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 1 cut(s) 1003
SfaNI GCATC 4 cut(s) 67, 100, 787, 812
SmiMI CAYNNNNRTG 4 cut(s) 182, 188, 536, 557
SmlI CTYRAG 1 cut(s) 1002
SmoI CTYRAG 1 cut(s) 1002
SsiI CCGC 4 cut(s) 11, 428, 570, 962
SspMI CTAG 4 cut(s) 266, 446, 542, 828
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 3 cut(s) 142, 241, 859
TaiI ACGT 1 cut(s) 1098
TaqI TCGA 1 cut(s) 18
TauI GCSGC 3 cut(s) 13, 430, 573
TfiI GAWTC 2 cut(s) 433, 883
Tru1I TTAA 4 cut(s) 597, 875, 905, 1215
Tru9I TTAA 4 cut(s) 597, 875, 905, 1215
TseFI GTSAC 3 cut(s) 853, 941, 964
TseI GCWGC 4 cut(s) 26, 33, 833, 952
Tsp45I GTSAC 3 cut(s) 853, 941, 964
TspDTI ATGAA 5 cut(s) 286, 486, 541, 666, 880
TspGWI ACGGA 1 cut(s) 517
VspI ATTAAT 1 cut(s) 1215
XapI RAATTY 3 cut(s) 357, 750, 1114
XceI RCATGY 3 cut(s) 367, 382, 981
XmnI GAANNNNTTC 1 cut(s) 1083
XspI CTAG 4 cut(s) 266, 446, 542, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.