Rw1G037310

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000970
Physical Location & Seq
Reverse (-)
64977177 .. 64981082
3906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G037310.1

Sequence Viewer

Length: 1305 bp
ATGTTCGTCGGCCACATCGGAAACCTTGAGCTCTCCGCCGTCTCTATCTCTCTCTCCGTCATCTGTACCTTCTCTTTCGGCTTCATGCTTGGTATGGGAAGTGCACTAGAGACATTATGTGGTCAAGCATTTGGAGCTGGACGAGTTAACATGCTTGGTATCTACATGCAAAGGTCATGGATCATTCTATGGATAACCTGCCTTTTCCTCACTCCACTCTATTGCTTCTCTGCCCCAATCTTAAAGCTTCTTGGCCAAGATGACGAGGTAGCGGACCTGGCGGGAACATTCACCGTGTATGTGATCCCTCAGCTATTCTCACTTGCCATCAATTTCCCTTGCCAAAAGTTCCTGCAGGCACAAAGCAAGGTCATAGTTCTTGCTTGGATCGGATTCATAGCCCTAATGGTTCACATTGGACTTCTTTTCCTCTTCATTTACGTGTTCGATTGGGGGATATATGGTGCAGCTATAGCTTTTGACATTACAGGTTGGGAAATTGTGATTGCTGAGCTTGTTTATATACTTGGTTGGTGTAACGAAGGGTGGAGCGGGTTTTCATGGTTGGCTTTCAAAGACATTTGGGCCTTTGTGAAGCTTTCGCTTGAATCAGCTGTCATGCTTTGCCTTGAGATTTGGTATATGATGAGTATCATCATTCTTACTGGCCATCTTGACAATGCGGTCATTGCTCTTGATTCACTTTCTATTTGCATGAATGTCAATGGATGGGAAGCAATGCTGTTCATTGGAATCAATGCTGCTATTAGTGTTCGAGTTTCTAATGAACTTGGACTAGGGCGTCCGAGAGCAGCTAAATACTCAGTCTTTGTGACAGTGGCTCAATCTCTGCTGATTGGAATTTTTTGCATGGTTGTTATCTTGATTACCAAAGACTACTTCTCTGTCCTCTTCACAAGCAATGCCGAATTGCAAGCAGCTGTTTCAAAGCTAGCATGGCTTCTCGGAATCACCATGGTTCTCAACAGTGTCCAGCCTGTGATTTCAGGTGTTGCTATCGGAGGTGGATGGCAAGGAATTGTGGCTTATATTAACTTGGGTTGTTATTACATTTTTGGGCTCCCATTCGGATTCCTTCTTGGTTATACACTAGACATGGGAGTGACTGGCATTTGGGGAGGCATGATATGTGGAACAGCTCTTCAAACCTTGCTGCTCCTTATAATACTTTACAGAATCAACTGGAAAAAGGAGGTGGAAGAAAGTTCAGACCGAATGAAGAAATGGGGAGGTCAAGATATAAAAGGCGAGACAGTAGCTGAAAATAATCATAATATTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

434

Amino Acids

47.81

Weight (kDa)

5.04

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 142 1.3e-28 MatE
MatE PF01554 205 - 366 2.2e-22 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1184
AasI GACNNNNNNGTC 2 cut(s) 683, 801
Acc36I ACCTGC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 552
AciI CCGC 5 cut(s) 36, 272, 281, 552, 683
AclWI GGATC 3 cut(s) 188, 298, 395
AcoI YGGCCR 3 cut(s) 10, 253, 667
AcsI RAATTY 1 cut(s) 861
AcyI GRCGYC 1 cut(s) 802
AfaI GTAC 1 cut(s) 67
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 4 cut(s) 574, 608, 948, 1166
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 2 cut(s) 884, 916
Alw21I GWGCWC 2 cut(s) 33, 106
Alw26I GTCTC 3 cut(s) 46, 104, 1265
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 3 cut(s) 188, 298, 395
AlwNI CAGNNNCTG 1 cut(s) 1280
AoxI GGCC 4 cut(s) 10, 253, 585, 667
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 5 cut(s) 467, 761, 812, 938, 1174
ApoI RAATTY 1 cut(s) 861
AspS9I GGNCC 2 cut(s) 274, 585
AsuHPI GGTGA 2 cut(s) 283, 964
AsuNHI GCTAGC 1 cut(s) 952
AvaII GGWCC 1 cut(s) 274
BaeGI GKGCMC 1 cut(s) 106
BalI TGGCCA 2 cut(s) 255, 669
BanII GRGCYC 2 cut(s) 33, 1083
Bbv12I GWGCWC 2 cut(s) 33, 106
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 5 cut(s) 479, 748, 824, 950, 1161
BccI CCATC 4 cut(s) 335, 678, 723, 1023
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 278
BcoDI GTCTC 3 cut(s) 46, 104, 1265
BfaI CTAG 4 cut(s) 107, 797, 953, 1112
BfmI CTRYAG 2 cut(s) 353, 471
BfuAI ACCTGC 1 cut(s) 206
BisI GCNGC 5 cut(s) 468, 762, 813, 939, 1175
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 5 cut(s) 469, 763, 814, 940, 1176
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 274, 585
BmiI GGNNCC 1 cut(s) 1082
BmrFI CCNGG 1 cut(s) 278
BmtI GCTAGC 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 309
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 2 cut(s) 47, 650
BsaAI YACGTR 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 650
BsaHI GRCGYC 1 cut(s) 802
BsaJI CCNNGG 1 cut(s) 975
Bse1I ACTGG 3 cut(s) 670, 1132, 1208
Bse3DI GCAATG 3 cut(s) 687, 744, 928
Bse8I GATNNNNATC 1 cut(s) 650
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 975
BseGI GGATG 2 cut(s) 734, 1034
BseJI GATNNNNATC 1 cut(s) 650
BseMI GCAATG 3 cut(s) 687, 744, 928
BseMII CTCAG 3 cut(s) 323, 501, 837
BseNI ACTGG 3 cut(s) 670, 1132, 1208
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 5 cut(s) 479, 748, 824, 950, 1161
BsgI GTGCAG 1 cut(s) 486
BshFI GGCC 4 cut(s) 12, 255, 587, 669
BsiHKAI GWGCWC 2 cut(s) 33, 106
BsmAI GTCTC 3 cut(s) 46, 104, 1265
BsmBI CGTCTC 1 cut(s) 46
BsnI GGCC 4 cut(s) 12, 255, 587, 669
Bsp1286I GDGCHC 3 cut(s) 33, 106, 1083
Bsp143I GATC 3 cut(s) 180, 303, 387
Bsp1720I GCTNAGC 1 cut(s) 510
Bsp19I CCATGG 1 cut(s) 975
BspACI CCGC 5 cut(s) 36, 272, 281, 552, 683
BspANI GGCC 4 cut(s) 12, 255, 587, 669
BspCNI CTCAG 3 cut(s) 322, 502, 836
BspLI GGNNCC 1 cut(s) 1082
BspMAI CTGCAG 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 206
BspOI GCTAGC 1 cut(s) 956
BspPI GGATC 3 cut(s) 188, 298, 395
BspQI GCTCTTC 1 cut(s) 1167
BsrBI CCGCTC 1 cut(s) 552
BsrDI GCAATG 3 cut(s) 687, 744, 928
BsrI ACTGG 3 cut(s) 670, 1132, 1208
BssECI CCNNGG 1 cut(s) 975
BssMI GATC 3 cut(s) 180, 303, 387
BssNI GRCGYC 1 cut(s) 802
BssT1I CCWWGG 1 cut(s) 975
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 4 cut(s) 295, 838, 989, 1276
Bst6I CTCTTC 3 cut(s) 437, 917, 1167
BstACI GRCGYC 1 cut(s) 802
BstBAI YACGTR 1 cut(s) 442
BstC8I GCNNGC 3 cut(s) 357, 936, 954
BstDEI CTNAG 3 cut(s) 309, 510, 823
BstDSI CCRYGG 1 cut(s) 975
BstF5I GGATG 2 cut(s) 734, 1034
BstKTI GATC 3 cut(s) 183, 306, 390
BstMAI GTCTC 3 cut(s) 46, 104, 1265
BstMBI GATC 3 cut(s) 180, 303, 387
BstMWI GCNNNNNNNGC 5 cut(s) 134, 278, 473, 689, 958
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 2 cut(s) 154, 169
BstSCI CCNGG 1 cut(s) 276
BstSFI CTRYAG 2 cut(s) 353, 471
BstSLI GKGCMC 1 cut(s) 106
BstV1I GCAGC 5 cut(s) 479, 748, 824, 950, 1161
BsuRI GGCC 4 cut(s) 12, 255, 587, 669
BtgI CCRYGG 1 cut(s) 975
BtsCI GGATG 2 cut(s) 734, 1034
BtsIMutI CAGTG 2 cut(s) 843, 994
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 3 cut(s) 357, 936, 954
CaiI CAGNNNCTG 1 cut(s) 1280
Cfr13I GGNCC 2 cut(s) 274, 585
CseI GACGC 1 cut(s) 791
Csp6I GTAC 1 cut(s) 66
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 3 cut(s) 309, 510, 823
DpnI GATC 3 cut(s) 182, 305, 389
DpnII GATC 3 cut(s) 180, 303, 387
DrdI GACNNNNNNGTC 2 cut(s) 683, 801
DseDI GACNNNNNNGTC 2 cut(s) 683, 801
EaeI YGGCCR 3 cut(s) 10, 253, 667
Eam1104I CTCTTC 3 cut(s) 437, 917, 1167
EarI CTCTTC 3 cut(s) 437, 917, 1167
EciI GGCGGA 1 cut(s) 25
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 975
Eco24I GRGCYC 2 cut(s) 33, 1083
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 975
EcoT38I GRGCYC 2 cut(s) 33, 1083
ErhI CCWWGG 1 cut(s) 975
Esp3I CGTCTC 1 cut(s) 46
FauI CCCGC 2 cut(s) 274, 545
Fnu4HI GCNGC 5 cut(s) 468, 762, 813, 939, 1175
FokI GGATG 2 cut(s) 741, 1041
FriOI GRGCYC 2 cut(s) 33, 1083
Fsp4HI GCNGC 5 cut(s) 468, 762, 813, 939, 1175
FspBI CTAG 4 cut(s) 107, 797, 953, 1112
GluI GCNGC 5 cut(s) 468, 762, 813, 939, 1175
HaeIII GGCC 4 cut(s) 12, 255, 587, 669
HgaI GACGC 1 cut(s) 791
Hin1I GRCGYC 1 cut(s) 802
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 245, 596
HinfI GANTC 7 cut(s) 393, 608, 698, 753, 969, 1092, 1197
HpaI GTTAAC 1 cut(s) 148
HphI GGTGA 2 cut(s) 283, 964
Hpy166II GTNNAC 3 cut(s) 104, 148, 412
Hpy188I TCNGA 7 cut(s) 20, 392, 807, 968, 1022, 1091, 1231
Hpy188III TCNNGA 4 cut(s) 674, 695, 883, 1256
Hpy8I GTNNAC 3 cut(s) 104, 148, 412
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 3 cut(s) 79, 536, 1106
HpyCH4III ACNGT 4 cut(s) 295, 838, 989, 1276
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 7 cut(s) 104, 169, 355, 467, 714, 870, 934
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 278, 473, 689, 958
HpyF3I CTNAG 3 cut(s) 309, 510, 823
HpySE526I ACGT 1 cut(s) 441
Hsp92I GRCGYC 1 cut(s) 802
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 3 cut(s) 180, 303, 387
LguI GCTCTTC 1 cut(s) 1167
LmnI GCTCC 4 cut(s) 134, 549, 1086, 1182
Lsp1109I GCAGC 5 cut(s) 479, 748, 824, 950, 1161
MaeI CTAG 4 cut(s) 107, 797, 953, 1112
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 3 cut(s) 536, 832, 1123
MalI GATC 3 cut(s) 182, 305, 389
MbiI CCGCTC 1 cut(s) 552
MboI GATC 3 cut(s) 180, 303, 387
MboII GAAGA 5 cut(s) 424, 904, 1154, 1232, 1252
MhlI GDGCHC 3 cut(s) 33, 106, 1083
MlsI TGGCCA 2 cut(s) 255, 669
MluCI AATT 5 cut(s) 331, 498, 861, 929, 1038
MluNI TGGCCA 2 cut(s) 255, 669
MnlI CCTC 9 cut(s) 218, 259, 318, 440, 920, 1016, 1133, 1207, 1244
Mox20I TGGCCA 2 cut(s) 255, 669
MscI TGGCCA 2 cut(s) 255, 669
MseI TTAA 3 cut(s) 147, 242, 1053
MslI CAYNNNNRTG 2 cut(s) 440, 1121
Msp20I TGGCCA 2 cut(s) 255, 669
MspA1I CMGCKG 2 cut(s) 614, 941
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 5 cut(s) 134, 278, 473, 689, 958
NcoI CCATGG 1 cut(s) 975
NdeII GATC 3 cut(s) 180, 303, 387
NheI GCTAGC 1 cut(s) 952
NlaIV GGNNCC 1 cut(s) 1082
NmuCI GTSAC 2 cut(s) 832, 1123
NspI RCATGY 2 cut(s) 154, 169
PciSI GCTCTTC 1 cut(s) 1167
PfeI GAWTC 7 cut(s) 393, 608, 698, 753, 969, 1092, 1197
PkrI GCNGC 5 cut(s) 469, 763, 814, 940, 1176
Ppu21I YACGTR 1 cut(s) 442
PsiI TTATAA 1 cut(s) 1184
Psp124BI GAGCTC 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 1082
PspPI GGNCC 2 cut(s) 274, 585
PstI CTGCAG 1 cut(s) 357
PstNI CAGNNNCTG 1 cut(s) 1280
PvuII CAGCTG 2 cut(s) 614, 941
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 2 cut(s) 440, 1121
SacI GAGCTC 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 1167
SaqAI TTAA 3 cut(s) 147, 242, 1053
SatI GCNGC 5 cut(s) 468, 762, 813, 939, 1175
Sau3AI GATC 3 cut(s) 180, 303, 387
Sau96I GGNCC 2 cut(s) 274, 585
SbfI CCTGCAGG 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 278
SdaI CCTGCAGG 1 cut(s) 357
SduI GDGCHC 3 cut(s) 33, 106, 1083
SfcI CTRYAG 2 cut(s) 353, 471
SinI GGWCC 1 cut(s) 274
SmiMI CAYNNNNRTG 2 cut(s) 440, 1121
SmlI CTYRAG 2 cut(s) 26, 629
SmoI CTYRAG 2 cut(s) 26, 629
Sse8387I CCTGCAGG 1 cut(s) 357
Sse9I AATT 5 cut(s) 331, 498, 861, 929, 1038
SsiI CCGC 5 cut(s) 36, 272, 281, 552, 683
SspI AATATT 1 cut(s) 1297
SspMI CTAG 4 cut(s) 107, 797, 953, 1112
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 975
TaaI ACNGT 4 cut(s) 295, 838, 989, 1276
TaiI ACGT 1 cut(s) 444
TaqI TCGA 2 cut(s) 447, 775
TaqII GACCGA 1 cut(s) 1248
TasI AATT 5 cut(s) 331, 498, 861, 929, 1038
TfiI GAWTC 7 cut(s) 393, 608, 698, 753, 969, 1092, 1197
Tru1I TTAA 3 cut(s) 147, 242, 1053
Tru9I TTAA 3 cut(s) 147, 242, 1053
TscAI CASTG 2 cut(s) 843, 994
TseFI GTSAC 2 cut(s) 832, 1123
TseI GCWGC 5 cut(s) 467, 761, 812, 938, 1174
Tsp45I GTSAC 2 cut(s) 832, 1123
TspDTI ATGAA 8 cut(s) 73, 385, 424, 549, 731, 736, 801, 1253
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 2 cut(s) 843, 994
VneI GTGCAC 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 274
XapI RAATTY 1 cut(s) 861
XceI RCATGY 2 cut(s) 154, 169
XspI CTAG 4 cut(s) 107, 797, 953, 1112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.