MD01G1206300.v1.1

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000970
Physical Location & Seq
Reverse (-)
30165178 .. 30169639
4462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1206300.v1.1.491

Sequence Viewer

Length: 1473 bp
ATGGAGACGCCACTGCTTCACGGAGTGAGCGACGGCGAGTTCCATGACTACGAGCCGGTGAGGAGCTTCAAGGATGCTCGGTCGGTGGCGTGGACTGAAACCAAGAAGCTGTGGAAGATTGCAGGGCCCATCGCCTTCACTATCATCTGCAACTATGGAACCAACACTGCTTCCACCATGTTTGTCGGCCATCTCGGAAACCTAGAGCTCTCCGCCGTCTCCATCTCTCTCTCCGTCATCAGCACCTTCTCATTCGGTTTCATGCTTGGCATGGGGAGTGCATTAGAGACGCTTTGTGGCCAAGCTTTCGGGGCCGGACGTGTGCACATGCTCGGCGTTTACATGCAAAGATCATGGATCATTCTGTTCACGAGCTGTGTTATACTCACGCCACTCTATGTCTTCTCCGGCCCGATTTTAAAGCTTCTTGGGCAGGAAGATGACGTGGCTAATCTTGCCGGATCATTCACTAGATATTGCATACCGCAGTTGTTCTCACTTGCCTTCAACTTCCCTGCCCAGAAGTTCCTTCAGTCTCAAAGCAAAGTTCTCGTGCTTATGTGGATTGGATTCATCGCGCTTGTTGTACACATTGGATGGCTTTTTCTATTCGTTTACGTGTTCGATTGGGGTATTTATGGTATAGGCATTGCGTTTAGCTTGACAGGTTGGGAAACTGTGATTGCTCAAAATATTTACATAATGAACTGGTGCAAGGAAGGGTGGACAGGGTTTTCTTGGTTGGCGTTCAAAGACATATGGGCGTTTGTCCGGCTATCACTTGAGTCGGCCGTGATGCTTTGCCTGGAGATTTGGTATATGATGAGTATAACTCTTGTTACAGGCCACCTTAATAATGCCGTGCTTGCTATTGATTCGCTCACTATTTGCTTAAATTTCAACGGTTTGGAAGCAATGTTGTTCATTGGCATCAATGCTGCTATAAGTGTTCGAGTCTCCAACGAACTTGGATTGGGACGTCCAAGAACAGCTAAATATGCAGTCTACGTGACGGTTGGTCAGTCTCTGCTCTTCGGAATCATTTGCATGATTCTGATCTTGATAACCAAAGACTACTTTGCTGTCCTGTTCACAACCAGCAAGGAATTGCAACATTCTGTTTCCAATCTAGCAGTTCTTCTCGGAGTGACTATGATTCTCAATAGCGTCCAACCAGTTATTTCGGGTGTTGCCATCGGAGGCGGATGGCAGGGACTGGTGGCTTATATAAACTTGGGTTCTTACTACATCTTCGGGCTTCCACTCGGTATCGTTCTTGGCTATGTAGCAGATTGGGGAGTGAAGGGAATTTGGGGAGGCATGATATGCGGGACTGCTCTTCAAACATTGATGCTTGTGATTATACTCTACAGAACCAACTGGACTAAGGAGGTTGAACAAACAACAGACCGGATGAAGAAGTGGGCCGGGCAGAACTACGGCGACGAGAAGATAGATGGTGTTAGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

491

Amino Acids

54.11

Weight (kDa)

6.39

Isoelectric Point (pI)

24.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 43 - 198 1.5e-36 MatE
MatE PF01554 264 - 424 3.4e-21 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 982
AccI GTMKAC 1 cut(s) 1005
AccII CGCG 1 cut(s) 578
AciI CCGC 4 cut(s) 213, 485, 1203, 1329
AclWI GGATC 2 cut(s) 365, 469
AcoI YGGCCR 3 cut(s) 187, 298, 789
AcsI RAATTY 2 cut(s) 895, 1308
AcuI CTGAAG 1 cut(s) 515
AcyI GRCGYC 2 cut(s) 8, 979
AdeI CACNNNGTG 1 cut(s) 25
AfaI GTAC 1 cut(s) 588
AflIII ACRYGT 2 cut(s) 319, 618
AgsI TTSAA 6 cut(s) 70, 508, 751, 901, 1343, 1397
AhdI GACNNNNNGTC 1 cut(s) 1017
AjiI CACGTC 2 cut(s) 320, 445
AjnI CCWGG 1 cut(s) 804
AluBI AGCT 8 cut(s) 66, 109, 208, 305, 375, 424, 660, 992
AluI AGCT 8 cut(s) 66, 109, 208, 305, 375, 424, 660, 992
Alw21I GWGCWC 2 cut(s) 210, 327
Alw26I GTCTC 5 cut(s) 223, 281, 540, 961, 1029
Alw44I GTGCAC 1 cut(s) 323
AlwI GGATC 2 cut(s) 365, 469
AlwNI CAGNNNCTG 2 cut(s) 1027, 1216
AoxI GGCC 8 cut(s) 125, 187, 298, 312, 409, 789, 844, 1425
ApaI GGGCCC 1 cut(s) 129
ApaLI GTGCAC 1 cut(s) 323
ApeKI GCWGC 1 cut(s) 938
ApoI RAATTY 2 cut(s) 895, 1308
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 5 cut(s) 125, 126, 312, 410, 1425
AsuC2I CCSGG 1 cut(s) 1429
AsuHPI GGTGA 1 cut(s) 70
BaeGI GKGCMC 2 cut(s) 129, 327
BalI TGGCCA 1 cut(s) 300
BanII GRGCYC 2 cut(s) 129, 210
BauI CACGAG 2 cut(s) 370, 551
BbsI GAAGAC 1 cut(s) 394
Bbv12I GWGCWC 2 cut(s) 210, 327
BbvI GCAGC 1 cut(s) 925
BccI CCATC 7 cut(s) 137, 198, 230, 591, 1200, 1202, 1451
BceAI ACGGC 5 cut(s) 49, 200, 776, 845, 1456
BcgI CGANNNNNNTGC 2 cut(s) 532, 566
BciT130I CCWGG 1 cut(s) 806
BcnI CCSGG 1 cut(s) 1429
BcoDI GTCTC 5 cut(s) 223, 281, 540, 961, 1029
BfaI CTAG 3 cut(s) 203, 471, 1130
BfmI CTRYAG 1 cut(s) 1369
BisI GCNGC 1 cut(s) 939
BlsI GCNGC 1 cut(s) 940
Bme1390I CCNGG 2 cut(s) 806, 1429
BmeRI GACNNNNNGTC 1 cut(s) 1017
BmgBI CACGTC 2 cut(s) 320, 445
BmgT120I GGNCC 5 cut(s) 125, 126, 312, 410, 1425
BmiI GGNNCC 3 cut(s) 127, 160, 313
BmrFI CCNGG 2 cut(s) 806, 1429
BmsI GCATC 4 cut(s) 64, 786, 939, 1341
BpiI GAAGAC 1 cut(s) 394
BplI GAGNNNNNCTC 2 cut(s) 817, 849
BpmI CTGGAG 1 cut(s) 827
BpuEI CTTGAG 1 cut(s) 803
BpuMI CCSGG 1 cut(s) 1429
BsaAI YACGTR 2 cut(s) 619, 1009
BsaBI GATNNNNATC 1 cut(s) 1055
BsaHI GRCGYC 2 cut(s) 8, 979
BsaWI WCCGGW 1 cut(s) 1410
BsaXI ACNNNNNCTCC 2 cut(s) 800, 830
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 4 cut(s) 713, 1175, 1221, 1385
Bse3DI GCAATG 2 cut(s) 648, 921
Bse8I GATNNNNATC 1 cut(s) 1055
BseBI CCWGG 1 cut(s) 806
BseGI GGATG 4 cut(s) 79, 602, 1211, 1419
BseJI GATNNNNATC 1 cut(s) 1055
BseMI GCAATG 2 cut(s) 648, 921
BseNI ACTGG 4 cut(s) 713, 1175, 1221, 1385
BseRI GAGGAG 1 cut(s) 76
BseSI GKGCMC 2 cut(s) 129, 327
BseX3I CGGCCG 1 cut(s) 789
BseXI GCAGC 1 cut(s) 925
Bsh1236I CGCG 1 cut(s) 578
Bsh1285I CGRYCG 2 cut(s) 83, 792
BshFI GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
BsiEI CGRYCG 2 cut(s) 83, 792
BsiHKAI GWGCWC 2 cut(s) 210, 327
BsiSI CCGG 7 cut(s) 56, 315, 408, 459, 772, 1411, 1428
BslFI GGGAC 3 cut(s) 990, 1227, 1345
BsmAI GTCTC 5 cut(s) 223, 281, 540, 961, 1029
BsmBI CGTCTC 2 cut(s) 223, 281
BsmFI GGGAC 3 cut(s) 990, 1227, 1345
BsnI GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
Bsp120I GGGCCC 1 cut(s) 125
Bsp1286I GDGCHC 3 cut(s) 129, 210, 327
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 4 cut(s) 350, 357, 461, 1056
BspACI CCGC 4 cut(s) 213, 485, 1203, 1329
BspANI GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 3 cut(s) 127, 160, 313
BspPI GGATC 2 cut(s) 365, 469
BspQI GCTCTTC 2 cut(s) 1037, 1344
BsrDI GCAATG 2 cut(s) 648, 921
BsrFI RCCGGY 1 cut(s) 55
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 4 cut(s) 713, 1175, 1221, 1385
BssAI RCCGGY 1 cut(s) 55
BssMI GATC 4 cut(s) 350, 357, 461, 1056
BssNI GRCGYC 2 cut(s) 8, 979
BssSI CACGAG 2 cut(s) 370, 551
Bst2BI CACGAG 2 cut(s) 370, 551
Bst2UI CCWGG 1 cut(s) 806
Bst4CI ACNGT 3 cut(s) 679, 905, 1015
Bst6I CTCTTC 2 cut(s) 1037, 1344
BstACI GRCGYC 2 cut(s) 8, 979
BstAPI GCANNNNNTGC 1 cut(s) 1326
BstAUI TGTACA 1 cut(s) 586
BstBAI YACGTR 2 cut(s) 619, 1009
BstC8I GCNNGC 1 cut(s) 867
BstDEI CTNAG 1 cut(s) 1386
BstF5I GGATG 4 cut(s) 79, 602, 1211, 1419
BstFNI CGCG 1 cut(s) 578
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 4 cut(s) 353, 360, 464, 1059
BstMAI GTCTC 5 cut(s) 223, 281, 540, 961, 1029
BstMBI GATC 4 cut(s) 350, 357, 461, 1056
BstMCI CGRYCG 2 cut(s) 83, 792
BstMWI GCNNNNNNNGC 6 cut(s) 311, 430, 455, 866, 998, 1326
BstNI CCWGG 1 cut(s) 806
BstNSI RCATGY 2 cut(s) 331, 346
BstSCI CCNGG 2 cut(s) 804, 1427
BstSFI CTRYAG 1 cut(s) 1369
BstSLI GKGCMC 2 cut(s) 129, 327
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 1 cut(s) 925
BstV2I GAAGAC 1 cut(s) 394
BstZI CGGCCG 1 cut(s) 789
BsuRI GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
BtgZI GCGATG 2 cut(s) 115, 559
BtrI CACGTC 2 cut(s) 320, 445
BtsCI GGATG 4 cut(s) 79, 602, 1211, 1419
BtsI GCAGTG 2 cut(s) 11, 165
BtsIMutI CAGTG 2 cut(s) 11, 165
Cac8I GCNNGC 1 cut(s) 867
CaiI CAGNNNCTG 2 cut(s) 1027, 1216
CfoI GCGC 1 cut(s) 580
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 5 cut(s) 125, 126, 312, 410, 1425
CseI GACGC 3 cut(s) 16, 298, 1156
Csp6I GTAC 1 cut(s) 587
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 9 cut(s) 44, 178, 262, 271, 328, 343, 354, 1048, 1321
CviQI GTAC 1 cut(s) 587
DdeI CTNAG 1 cut(s) 1386
DpnI GATC 4 cut(s) 352, 359, 463, 1058
DpnII GATC 4 cut(s) 350, 357, 461, 1056
DraI TTTAAA 1 cut(s) 420
DraIII CACNNNGTG 1 cut(s) 25
DriI GACNNNNNGTC 1 cut(s) 1017
EaeI YGGCCR 3 cut(s) 187, 298, 789
EagI CGGCCG 1 cut(s) 789
Eam1104I CTCTTC 2 cut(s) 1037, 1344
Eam1105I GACNNNNNGTC 1 cut(s) 1017
EarI CTCTTC 2 cut(s) 1037, 1344
EciI GGCGGA 2 cut(s) 202, 1218
Ecl136II GAGCTC 1 cut(s) 208
EclXI CGGCCG 1 cut(s) 789
Eco24I GRGCYC 2 cut(s) 129, 210
Eco52I CGGCCG 1 cut(s) 789
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 804
EcoT38I GRGCYC 2 cut(s) 129, 210
Esp3I CGTCTC 2 cut(s) 223, 281
FaeI CATG 9 cut(s) 47, 181, 265, 274, 331, 346, 357, 1051, 1324
FaqI GGGAC 3 cut(s) 990, 1227, 1345
FatI CATG 9 cut(s) 43, 177, 261, 270, 327, 342, 353, 1047, 1320
FauI CCCGC 1 cut(s) 1322
FauNDI CATATG 1 cut(s) 758
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 1 cut(s) 939
FokI GGATG 4 cut(s) 86, 609, 1218, 1426
FriOI GRGCYC 2 cut(s) 129, 210
Fsp4HI GCNGC 1 cut(s) 939
FspBI CTAG 3 cut(s) 203, 471, 1130
GlaI GCGC 1 cut(s) 579
GluI GCNGC 1 cut(s) 939
GsuI CTGGAG 1 cut(s) 827
HaeIII GGCC 8 cut(s) 127, 189, 300, 314, 411, 791, 846, 1427
HapII CCGG 7 cut(s) 56, 315, 408, 459, 772, 1411, 1428
HgaI GACGC 3 cut(s) 16, 298, 1156
HhaI GCGC 1 cut(s) 580
Hin1I GRCGYC 2 cut(s) 8, 979
Hin1II CATG 9 cut(s) 47, 181, 265, 274, 331, 346, 357, 1051, 1324
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HindIII AAGCTT 2 cut(s) 303, 422
HinfI GANTC 7 cut(s) 570, 785, 875, 954, 1038, 1051, 1156
HpaII CCGG 7 cut(s) 56, 315, 408, 459, 772, 1411, 1428
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 9 cut(s) 93, 325, 340, 369, 589, 616, 726, 1006, 1092
Hpy188I TCNGA 5 cut(s) 197, 1037, 1056, 1145, 1199
Hpy188III TCNNGA 2 cut(s) 370, 1060
Hpy8I GTNNAC 9 cut(s) 93, 325, 340, 369, 589, 616, 726, 1006, 1092
Hpy99I CGWCG 2 cut(s) 35, 1448
HpyAV CCTTC 6 cut(s) 145, 256, 514, 539, 713, 1297
HpyCH4III ACNGT 3 cut(s) 679, 905, 1015
HpyCH4IV ACGT 5 cut(s) 319, 444, 618, 979, 1008
HpyF10VI GCNNNNNNNGC 6 cut(s) 311, 430, 455, 866, 998, 1326
HpyF3I CTNAG 1 cut(s) 1386
HpySE526I ACGT 5 cut(s) 319, 444, 618, 979, 1008
Hsp92I GRCGYC 2 cut(s) 8, 979
Hsp92II CATG 9 cut(s) 47, 181, 265, 274, 331, 346, 357, 1051, 1324
HspAI GCGC 1 cut(s) 578
Kzo9I GATC 4 cut(s) 350, 357, 461, 1056
LguI GCTCTTC 2 cut(s) 1037, 1344
LmnI GCTCC 1 cut(s) 63
Lsp1109I GCAGC 1 cut(s) 925
LweI GCATC 4 cut(s) 64, 786, 939, 1341
MaeI CTAG 3 cut(s) 203, 471, 1130
MaeII ACGT 5 cut(s) 319, 444, 618, 979, 1008
MaeIII GTNAC 3 cut(s) 838, 1009, 1147
MalI GATC 4 cut(s) 352, 359, 463, 1058
MboI GATC 4 cut(s) 350, 357, 461, 1056
MboII GAAGA 9 cut(s) 127, 394, 449, 1024, 1130, 1243, 1331, 1429, 1462
MhlI GDGCHC 3 cut(s) 129, 210, 327
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 3 cut(s) 895, 1106, 1308
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 2 cut(s) 794, 963
MmeI TCCRAC 2 cut(s) 984, 1195
MnlI CCTC 4 cut(s) 54, 1193, 1310, 1384
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MseI TTAA 3 cut(s) 419, 852, 893
MslI CAYNNNNRTG 1 cut(s) 1046
Msp20I TGGCCA 1 cut(s) 300
MspI CCGG 7 cut(s) 56, 315, 408, 459, 772, 1411, 1428
MspR9I CCNGG 2 cut(s) 806, 1429
MvaI CCWGG 1 cut(s) 806
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 6 cut(s) 311, 430, 455, 866, 998, 1326
NciI CCSGG 1 cut(s) 1429
NdeI CATATG 1 cut(s) 758
NdeII GATC 4 cut(s) 350, 357, 461, 1056
NlaIII CATG 9 cut(s) 47, 181, 265, 274, 331, 346, 357, 1051, 1324
NlaIV GGNNCC 3 cut(s) 127, 160, 313
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 2 cut(s) 1009, 1147
NspI RCATGY 2 cut(s) 331, 346
PciSI GCTCTTC 2 cut(s) 1037, 1344
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PfeI GAWTC 5 cut(s) 570, 875, 1038, 1051, 1156
PkrI GCNGC 1 cut(s) 940
PleI GAGTC 2 cut(s) 793, 962
PpsI GAGTC 2 cut(s) 793, 962
Ppu21I YACGTR 2 cut(s) 619, 1009
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 804
PspGI CCWGG 1 cut(s) 804
PspN4I GGNNCC 3 cut(s) 127, 160, 313
PspOMI GGGCCC 1 cut(s) 125
PspPI GGNCC 5 cut(s) 125, 126, 312, 410, 1425
PstNI CAGNNNCTG 2 cut(s) 1027, 1216
RsaI GTAC 1 cut(s) 588
RsaNI GTAC 1 cut(s) 587
RseI CAYNNNNRTG 1 cut(s) 1046
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 2 cut(s) 1037, 1344
SaqAI TTAA 3 cut(s) 419, 852, 893
SatI GCNGC 1 cut(s) 939
Sau3AI GATC 4 cut(s) 350, 357, 461, 1056
Sau96I GGNCC 5 cut(s) 125, 126, 312, 410, 1425
SchI GAGTC 2 cut(s) 794, 963
ScrFI CCNGG 2 cut(s) 806, 1429
SduI GDGCHC 3 cut(s) 129, 210, 327
SfaNI GCATC 4 cut(s) 64, 786, 939, 1341
SfcI CTRYAG 1 cut(s) 1369
SmiMI CAYNNNNRTG 1 cut(s) 1046
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 3 cut(s) 895, 1106, 1308
SsiI CCGC 4 cut(s) 213, 485, 1203, 1329
SspI AATATT 1 cut(s) 694
SspMI CTAG 3 cut(s) 203, 471, 1130
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 2 cut(s) 804, 1427
TaaI ACNGT 3 cut(s) 679, 905, 1015
TaiI ACGT 5 cut(s) 322, 447, 621, 982, 1011
TaqI TCGA 2 cut(s) 624, 952
TaqII GACCGA 1 cut(s) 69
TasI AATT 3 cut(s) 895, 1106, 1308
TatI WGTACW 1 cut(s) 586
TfiI GAWTC 5 cut(s) 570, 875, 1038, 1051, 1156
Tru1I TTAA 3 cut(s) 419, 852, 893
Tru9I TTAA 3 cut(s) 419, 852, 893
TscAI CASTG 2 cut(s) 18, 172
TseFI GTSAC 2 cut(s) 1009, 1147
TseI GCWGC 1 cut(s) 938
Tsp45I GTSAC 2 cut(s) 1009, 1147
TspDTI ATGAA 5 cut(s) 250, 562, 719, 913, 1430
TspGWI ACGGA 2 cut(s) 36, 223
TspRI CASTG 2 cut(s) 18, 172
VneI GTGCAC 1 cut(s) 323
XapI RAATTY 2 cut(s) 895, 1308
XceI RCATGY 2 cut(s) 331, 346
XmiI GTMKAC 1 cut(s) 1005
XspI CTAG 3 cut(s) 203, 471, 1130
ZraI GACGTC 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.