Prupe.2G298900_v2.0.a1

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000970
Physical Location & Seq
Reverse (-)
28818742 .. 28822326
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G298900.1

Sequence Viewer

Length: 1473 bp
ATGGAGGAGCCACTGCTTCCGGCAGTGAACGGCGATGGTGTTCGAGACTATGGGCCGGTGACGAGCTGGAAGGATGCTCGGTCGGTGACGTGGACAGAGACTAAGAAGCTGTGGAAAATAGGGGGGCCCATCGCCTTCACAATCATCTGCAACTTTGGTACCAACTCTGTTTCCACCATGTTCGTTGGACACCTCGGAAACCTTCAGCTCTCTGCCGTCTCAATCTCTCTCTCTGTGATCTCAGTCTTCTCTTTCGGCTTCATGCTCGGCATGGGCAGTGCATTAGAGACGCTTTGTGGTCAAGCATTTGGTGCTGGAAGGGTTAACATGCTTGGAGTGTACATGCAAAGGTCATGGATCATTCTATTGGCCAGCTGTGTCATCCTCACGCCAATCTACGTTTTCTCTGCCCCGATTTTAAAGCTTATTGGGCAGGACAAAGAAGTGGCTGATCTTGCTGGAAACTTCACCGTATGTGTCATTCCTCAGTTATTCTCACTTGCCATCATGTTCCCTTCCCAGAAGTTCCTTCAGGCACAAAGCAAGGTTGCTGTTCTTGCATGGATTGGTTTTCTGGCCCTTATTATACACATTGGATGGCTCTTTCTCTTCATTTACGTGTTGGATTGGGGCATCTATGGTGCAGCTATAGCATTTGATATCACAGGTTGGGAACTTGCGATTTGTCAAGTTATTTATATAATCGGTTGGTGCAATGAAGGGTGGAGTGGTTTTTCATGGTTAGCTTTCAAAGACATCTGGGCCTTTGTCAGGCTCTCGCTCGAATCAGCTGTCATGCTTTGCCTTGAGGTTTGGTATATGATGAGTATAATCATTCTTACTGGCCATCTTAGCAATGCTGTGATTTCTATTGATTCACTTTCTATCTGCATGAATCTCAATGGATGGGAAGGAATGTTGTTCATTGGAATTAATGCTGCTGTAAGTGTTCGAGTTTCCAATGAACTTGGATTGGGACGTCCAAGAGCAGCTAAATTCGCTGTCTTCGTGACAGTCGTTGAGTCTCTGCTCATTGGGATTGTTTGCATGATTGTCGTCTTGATAGCGAAAGACTACTTTTCTGTCATCTTCACAAGCAACGCGGAATTGCAACAAGCTGTTGCGAAGCTAGCATTTCTTCTTGGAATTACCATGGTTCTTAATAGTGTCCAACCCGTCATTTCGGGTGTTGCTATTGGAGGCGGATGGCAAGGACTGGTGGCTTATATCAACTTGGGTTGTTATTACATTTTTGGCCTCCCATTTGGATACCTTCTTGGTTATACAGCAAAATTGGGAGTTAAGGGAATTTGGGGAGGCATGATATGTGGAACTGCTCTTCAAACGTTGCTTCTCTTGATAGTGCTTTATAGAACCAACTGGAACAAGGAGGTTGAACAATCTTCGGAGCGAATGAAGAAGTGGGGTGGACAAAACGTTGAAGATGAGAAGATTGCTAACAACCGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

491

Amino Acids

53.62

Weight (kDa)

6.81

Isoelectric Point (pI)

27.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 982
Acc65I GGTACC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 1 cut(s) 1103
AciI CCGC 2 cut(s) 1103, 1203
AclI AACGTT 2 cut(s) 1346, 1437
AclWI GGATC 1 cut(s) 365
AcoI YGGCCR 2 cut(s) 369, 844
AcsI RAATTY 2 cut(s) 995, 1308
AcuI CTGAAG 2 cut(s) 188, 515
AcyI GRCGYC 1 cut(s) 979
AfaI GTAC 2 cut(s) 160, 341
AfiI CCNNNNNNNGG 1 cut(s) 771
AflIII ACRYGT 1 cut(s) 618
AgsI TTSAA 4 cut(s) 751, 1343, 1397, 1442
AjiI CACGTC 1 cut(s) 90
Alw26I GTCTC 5 cut(s) 39, 92, 223, 281, 1029
AlwI GGATC 1 cut(s) 365
AoxI GGCC 7 cut(s) 53, 125, 369, 576, 762, 844, 1255
ApaI GGGCCC 1 cut(s) 129
ApeKI GCWGC 3 cut(s) 644, 938, 989
ApoI RAATTY 2 cut(s) 995, 1308
ArsI GACNNNNNNTTYG 2 cut(s) 777, 809
AseI ATTAAT 1 cut(s) 933
Asp718I GGTACC 1 cut(s) 158
AspS9I GGNCC 5 cut(s) 53, 125, 126, 577, 762
AsuHPI GGTGA 3 cut(s) 70, 97, 460
AsuNHI GCTAGC 1 cut(s) 1129
BaeGI GKGCMC 1 cut(s) 129
BaeI ACNNNNGTAYC 2 cut(s) 142, 175
BalI TGGCCA 2 cut(s) 371, 846
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 129
BbsI GAAGAC 2 cut(s) 238, 997
BbvI GCAGC 3 cut(s) 656, 925, 1001
BccI CCATC 7 cut(s) 29, 137, 512, 591, 855, 900, 1200
BceAI ACGGC 2 cut(s) 46, 200
BcgI CGANNNNNNTGC 2 cut(s) 1036, 1070
BciVI GTATCC 1 cut(s) 1262
BcoDI GTCTC 5 cut(s) 39, 92, 223, 281, 1029
BfaI CTAG 1 cut(s) 1130
BfmI CTRYAG 1 cut(s) 648
BfuI GTATCC 1 cut(s) 1262
BisI GCNGC 3 cut(s) 645, 939, 990
BlsI GCNGC 3 cut(s) 646, 940, 991
BmgBI CACGTC 1 cut(s) 90
BmgT120I GGNCC 5 cut(s) 53, 125, 126, 577, 762
BmiI GGNNCC 4 cut(s) 9, 126, 127, 160
BmsI GCATC 2 cut(s) 64, 642
BmtI GCTAGC 1 cut(s) 1133
BpiI GAAGAC 2 cut(s) 238, 997
BpuEI CTTGAG 1 cut(s) 827
BsaAI YACGTR 1 cut(s) 619
BsaHI GRCGYC 1 cut(s) 979
BsaJI CCNNGG 2 cut(s) 193, 1152
Bsc4I CCNNNNNNNGG 1 cut(s) 771
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 847, 1221, 1385
Bse3DI GCAATG 2 cut(s) 721, 862
BseDI CCNNGG 2 cut(s) 193, 1152
BseGI GGATG 5 cut(s) 79, 381, 602, 911, 1211
BseLI CCNNNNNNNGG 1 cut(s) 771
BseMI GCAATG 2 cut(s) 721, 862
BseMII CTCAG 2 cut(s) 255, 500
BseNI ACTGG 3 cut(s) 847, 1221, 1385
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 3 cut(s) 656, 925, 1001
BsgI GTGCAG 1 cut(s) 663
Bsh1236I CGCG 1 cut(s) 1103
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 7 cut(s) 55, 127, 371, 578, 764, 846, 1257
BshNI GGYRCC 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 83
BsiSI CCGG 2 cut(s) 20, 56
BslFI GGGAC 1 cut(s) 990
BslI CCNNNNNNNGG 1 cut(s) 771
BsmAI GTCTC 5 cut(s) 39, 92, 223, 281, 1029
BsmBI CGTCTC 2 cut(s) 223, 281
BsmFI GGGAC 1 cut(s) 990
BsnI GGCC 7 cut(s) 55, 127, 371, 578, 764, 846, 1257
Bsp120I GGGCCC 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 129
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 3 cut(s) 237, 357, 451
Bsp19I CCATGG 1 cut(s) 1152
BspACI CCGC 2 cut(s) 1103, 1203
BspANI GGCC 7 cut(s) 55, 127, 371, 578, 764, 846, 1257
BspCNI CTCAG 2 cut(s) 254, 499
BspFNI CGCG 1 cut(s) 1103
BspLI GGNNCC 4 cut(s) 9, 126, 127, 160
BspOI GCTAGC 1 cut(s) 1133
BspPI GGATC 1 cut(s) 365
BspQI GCTCTTC 1 cut(s) 1344
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 721, 862
BsrFI RCCGGY 1 cut(s) 55
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 3 cut(s) 847, 1221, 1385
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 193, 1152
BssMI GATC 3 cut(s) 237, 357, 451
BssNI GRCGYC 1 cut(s) 979
BssT1I CCWWGG 1 cut(s) 1152
Bst4CI ACNGT 3 cut(s) 472, 1015, 1466
Bst6I CTCTTC 2 cut(s) 614, 1344
BstACI GRCGYC 1 cut(s) 979
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 373, 1131
BstDEI CTNAG 4 cut(s) 102, 241, 486, 851
BstDSI CCRYGG 1 cut(s) 1152
BstENI CCTNNNNNAGG 1 cut(s) 769
BstF5I GGATG 5 cut(s) 79, 381, 602, 911, 1211
BstFNI CGCG 1 cut(s) 1103
BstKTI GATC 3 cut(s) 240, 360, 454
BstMAI GTCTC 5 cut(s) 39, 92, 223, 281, 1029
BstMBI GATC 3 cut(s) 237, 357, 451
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 8 cut(s) 311, 430, 455, 557, 650, 852, 998, 1130
BstNSI RCATGY 2 cut(s) 331, 346
BstSFI CTRYAG 1 cut(s) 648
BstSLI GKGCMC 1 cut(s) 129
BstUI CGCG 1 cut(s) 1103
BstV1I GCAGC 3 cut(s) 656, 925, 1001
BstV2I GAAGAC 2 cut(s) 238, 997
BsuI GTATCC 1 cut(s) 1262
BsuRI GGCC 7 cut(s) 55, 127, 371, 578, 764, 846, 1257
BtgI CCRYGG 1 cut(s) 1152
BtgZI GCGATG 2 cut(s) 48, 115
BtrI CACGTC 1 cut(s) 90
BtsCI GGATG 5 cut(s) 79, 381, 602, 911, 1211
BtsI GCAGTG 3 cut(s) 11, 30, 283
BtsIMutI CAGTG 3 cut(s) 11, 30, 283
Cac8I GCNNGC 2 cut(s) 373, 1131
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 5 cut(s) 53, 125, 126, 577, 762
CseI GACGC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 159, 340
CviQI GTAC 2 cut(s) 159, 340
DdeI CTNAG 4 cut(s) 102, 241, 486, 851
DpnI GATC 3 cut(s) 239, 359, 453
DpnII GATC 3 cut(s) 237, 357, 451
DraI TTTAAA 1 cut(s) 420
EaeI YGGCCR 2 cut(s) 369, 844
Eam1104I CTCTTC 2 cut(s) 614, 1344
EarI CTCTTC 2 cut(s) 614, 1344
EciI GGCGGA 1 cut(s) 1218
Eco130I CCWWGG 1 cut(s) 1152
Eco24I GRGCYC 1 cut(s) 129
Eco32I GATATC 1 cut(s) 661
Eco57I CTGAAG 2 cut(s) 188, 515
EcoNI CCTNNNNNAGG 1 cut(s) 769
EcoO109I RGGNCCY 1 cut(s) 125
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 1152
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 1152
Esp3I CGTCTC 2 cut(s) 223, 281
FaqI GGGAC 1 cut(s) 990
Fnu4HI GCNGC 3 cut(s) 645, 939, 990
FokI GGATG 5 cut(s) 86, 368, 609, 918, 1218
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 3 cut(s) 645, 939, 990
FspBI CTAG 1 cut(s) 1130
GluI GCNGC 3 cut(s) 645, 939, 990
HaeIII GGCC 7 cut(s) 55, 127, 371, 578, 764, 846, 1257
HapII CCGG 2 cut(s) 20, 56
HgaI GACGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 979
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 4 cut(s) 785, 875, 895, 1022
HpaI GTTAAC 1 cut(s) 325
HpaII CCGG 2 cut(s) 20, 56
HphI GGTGA 3 cut(s) 70, 97, 460
Hpy166II GTNNAC 5 cut(s) 28, 93, 325, 340, 1430
Hpy188I TCNGA 2 cut(s) 197, 1408
Hpy188III TCNNGA 4 cut(s) 44, 1009, 1060, 1357
Hpy8I GTNNAC 5 cut(s) 28, 93, 325, 340, 1430
HpyAV CCTTC 9 cut(s) 64, 145, 212, 312, 525, 539, 713, 905, 1283
HpyCH4III ACNGT 3 cut(s) 472, 1015, 1466
HpyCH4IV ACGT 6 cut(s) 89, 399, 618, 979, 1346, 1437
HpyCH4V TGCA 9 cut(s) 150, 281, 346, 560, 644, 714, 891, 1047, 1111
HpyF10VI GCNNNNNNNGC 8 cut(s) 311, 430, 455, 557, 650, 852, 998, 1130
HpyF3I CTNAG 4 cut(s) 102, 241, 486, 851
HpySE526I ACGT 6 cut(s) 89, 399, 618, 979, 1346, 1437
Hsp92I GRCGYC 1 cut(s) 979
KpnI GGTACC 1 cut(s) 162
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 3 cut(s) 237, 357, 451
LguI GCTCTTC 1 cut(s) 1344
LmnI GCTCC 2 cut(s) 7, 1408
Lsp1109I GCAGC 3 cut(s) 656, 925, 1001
LweI GCATC 2 cut(s) 64, 642
MaeI CTAG 1 cut(s) 1130
MaeII ACGT 6 cut(s) 89, 399, 618, 979, 1346, 1437
MaeIII GTNAC 3 cut(s) 58, 85, 1009
MalI GATC 3 cut(s) 239, 359, 453
MboI GATC 3 cut(s) 237, 357, 451
MhlI GDGCHC 1 cut(s) 129
MlsI TGGCCA 2 cut(s) 371, 846
MluCI AATT 6 cut(s) 930, 995, 1106, 1146, 1292, 1308
MluNI TGGCCA 2 cut(s) 371, 846
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 3 cut(s) 166, 603, 1195
MnlI CCTC 8 cut(s) 203, 395, 495, 802, 1193, 1268, 1310, 1384
Mox20I TGGCCA 2 cut(s) 371, 846
MscI TGGCCA 2 cut(s) 371, 846
MseI TTAA 5 cut(s) 324, 419, 933, 1161, 1302
MslI CAYNNNNRTG 1 cut(s) 617
Msp20I TGGCCA 2 cut(s) 371, 846
MspA1I CMGCKG 2 cut(s) 375, 791
MspI CCGG 2 cut(s) 20, 56
MvnI CGCG 1 cut(s) 1103
MwoI GCNNNNNNNGC 8 cut(s) 311, 430, 455, 557, 650, 852, 998, 1130
NcoI CCATGG 1 cut(s) 1152
NdeII GATC 3 cut(s) 237, 357, 451
NheI GCTAGC 1 cut(s) 1129
NlaIV GGNNCC 4 cut(s) 9, 126, 127, 160
NmeAIII GCCGAG 1 cut(s) 246
NmuCI GTSAC 3 cut(s) 58, 85, 1009
NspI RCATGY 2 cut(s) 331, 346
PciSI GCTCTTC 1 cut(s) 1344
PcsI WCGNNNNNNNCGW 2 cut(s) 1005, 1014
PfeI GAWTC 3 cut(s) 785, 875, 895
PkrI GCNGC 3 cut(s) 646, 940, 991
PleI GAGTC 1 cut(s) 1030
PpsI GAGTC 1 cut(s) 1030
Ppu21I YACGTR 1 cut(s) 619
PshBI ATTAAT 1 cut(s) 933
Psp1406I AACGTT 2 cut(s) 1346, 1437
PspN4I GGNNCC 4 cut(s) 9, 126, 127, 160
PspOMI GGGCCC 1 cut(s) 125
PspPI GGNCC 5 cut(s) 53, 125, 126, 577, 762
PvuII CAGCTG 2 cut(s) 375, 791
RsaI GTAC 2 cut(s) 160, 341
RsaNI GTAC 2 cut(s) 159, 340
RseI CAYNNNNRTG 1 cut(s) 617
SapI GCTCTTC 1 cut(s) 1344
SaqAI TTAA 5 cut(s) 324, 419, 933, 1161, 1302
SatI GCNGC 3 cut(s) 645, 939, 990
Sau3AI GATC 3 cut(s) 237, 357, 451
Sau96I GGNCC 5 cut(s) 53, 125, 126, 577, 762
SchI GAGTC 1 cut(s) 1031
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 2 cut(s) 64, 642
SfcI CTRYAG 1 cut(s) 648
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
Sse9I AATT 6 cut(s) 930, 995, 1106, 1146, 1292, 1308
SsiI CCGC 2 cut(s) 1103, 1203
SspMI CTAG 1 cut(s) 1130
StyI CCWWGG 1 cut(s) 1152
TaaI ACNGT 3 cut(s) 472, 1015, 1466
TaiI ACGT 6 cut(s) 92, 402, 621, 982, 1349, 1440
TaqI TCGA 3 cut(s) 43, 783, 952
TaqII GACCGA 1 cut(s) 69
TasI AATT 6 cut(s) 930, 995, 1106, 1146, 1292, 1308
TatI WGTACW 1 cut(s) 339
TfiI GAWTC 3 cut(s) 785, 875, 895
Tru1I TTAA 5 cut(s) 324, 419, 933, 1161, 1302
Tru9I TTAA 5 cut(s) 324, 419, 933, 1161, 1302
TscAI CASTG 3 cut(s) 18, 30, 283
TseFI GTSAC 3 cut(s) 58, 85, 1009
TseI GCWGC 3 cut(s) 644, 938, 989
Tsp45I GTSAC 3 cut(s) 58, 85, 1009
TspDTI ATGAA 8 cut(s) 250, 601, 726, 732, 908, 913, 978, 1430
TspRI CASTG 3 cut(s) 18, 30, 283
VspI ATTAAT 1 cut(s) 933
XagI CCTNNNNNAGG 1 cut(s) 769
XapI RAATTY 2 cut(s) 995, 1308
XceI RCATGY 2 cut(s) 331, 346
XspI CTAG 1 cut(s) 1130
ZraI GACGTC 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.