MD07G1277000.v1.1

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000970
Physical Location & Seq
Reverse (-)
34232019 .. 34236081
4063 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1277000.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGGTGACGCCAGTGCCACTGCTTCACGCAGTGAACGGCGGCGAGTTCCATGACTACGAGCCGGTGAGGAACTTCAGGGATGCCCGGTCGGTGGCGTGGAATGAAACCAAGAAGCTGTGGAAAATTGCTGGGCCCATCGCCTTCACTATCATCTGCAACTTTGGCAACAACACAGCTACCACCATGTTTGTCGGCCATCTCGGAAACCTCCAGCTCTCCGCCGTTTCCATCTCTCTCTCCGTCATCTGCACCTTCTCATTCGGCTTCATGCTTGGCATGGGGAGTGCGTTAGAGACGCTTTGTGGCCAAGCTTTCGGAGCCGGACACGTTAACATGCTCGGCGTTTACATGCAAAGATCATGGATCATTCTGTTCACCAGCTGTGTTATCCTCACGCCAATCTATATCTTCTCCGCCCCGATTTTGAAGCTTCTCGGGCAGGATGATGACGTGGCTAATCTTGCTGGAACATTCACCATATATTGCATACCGCAGTTGTTCTCCCTTGCCTTCAACTTCCCTGCCCAGAAGTTCCTTCAGGCTCAAAGCAAAGTTCTCGTGCTTGCGTGGATTGGATTTATCTCGCTTCTTGTACAAATTGGAATGCTTTCTCTCTTCATTTACGTGTTCGATTGGGGTATATATGGTGCAGGCATTGCATTTGGCATCACAGGTTGGGTAATTGTGATTGCTCAAAATATTTACATAATGAAATGGTGCAAGGAAGGGTGGACAGGGTTTTCGTGGTTGGCGTTCAAAGACATATGGGCGTTTGTCCGGCTTTCACTTGAGTCCGCCGTGATGCTTTGCCTGGAGATTTGGTATATGATGACTCTTATCCTTGCTACAGGCCACCTTAGTAATGCCGTACTTTCTATTGACTCGCTCGCTATTTGCTTAAATTTCAACGGTTTGGAAGCAATGCTGTTCATTGGAATCAATGCTGCTATAAGTGTTCGGGTCTCCAACGAACTTGGATTGGCACGTCCAAGAGCAGCTAAATATTCAGTCTACGTGACGGTTGGTCAGTCTCTGCTCATCGGAATCGTTTGCATGGTTGTGATCTTGATAACCAAAGACTACTTTTCTGTCCTGTACACAAACAACGAGGAATTGCAACATTCAGTTTCCAATCTAGCAATTCTTCTCGGAGTGACTATGCTTCTCAATAGCGTCCAACCAGTTATTTCAGGTGTTGCTATCGGAGGCGGATGGCAGGGACTGGTGGCTTATGTAAACTTGGGTTGTTACTACATTATCGGGCTCCCGCTCGGTATCCTTCTTGGCTATGTAGCAAAATGGGGAGTGAAGGGAATTTGGGGAGGCATGATATGCGGGACTGCTCTTCAAACCTTGCTGCTTATGATTGTACTTTACAGAACCAACTGGAACAAGGAGGTTGAACAAACAACGGACCGGATGAAGAAGTGGGCTGGGCAAGACTACGGCGACGAGGAGATAGCTGCTGTTAGCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

493

Amino Acids

54.01

Weight (kDa)

6.59

Isoelectric Point (pI)

25.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 45 - 200 4.1e-32 MatE
MatE PF01554 266 - 426 1.5e-20 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1270
AccI GTMKAC 1 cut(s) 1011
AciI CCGC 8 cut(s) 39, 219, 414, 491, 795, 1209, 1268, 1335
AclWI GGATC 1 cut(s) 371
AcoI YGGCCR 2 cut(s) 193, 304
AcsI RAATTY 2 cut(s) 901, 1314
AcuI CTGAAG 2 cut(s) 58, 521
AcyI GRCGYC 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 31
AfaI GTAC 4 cut(s) 594, 870, 1097, 1371
AfiI CCNNNNNNNGG 1 cut(s) 91
AflIII ACRYGT 2 cut(s) 325, 624
AgsI TTSAA 6 cut(s) 427, 514, 757, 907, 1349, 1403
AhdI GACNNNNNGTC 1 cut(s) 1023
AjiI CACGTC 2 cut(s) 451, 986
AjnI CCWGG 1 cut(s) 810
AluBI AGCT 8 cut(s) 115, 176, 214, 311, 381, 430, 998, 1463
AluI AGCT 8 cut(s) 115, 176, 214, 311, 381, 430, 998, 1463
Alw26I GTCTC 3 cut(s) 287, 967, 1035
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 2 cut(s) 1033, 1222
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 4 cut(s) 131, 193, 304, 850
ApaI GGGCCC 1 cut(s) 135
ApeKI GCWGC 4 cut(s) 944, 995, 1357, 1463
ApoI RAATTY 2 cut(s) 901, 1314
ArsI GACNNNNNNTTYG 2 cut(s) 724, 756
Asp700I GAANNNNTTC 1 cut(s) 607
AspS9I GGNCC 3 cut(s) 131, 132, 1414
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 4 cut(s) 16, 76, 367, 466
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 1 cut(s) 1414
BaeGI GKGCMC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 306
BanII GRGCYC 2 cut(s) 135, 1266
BauI CACGAG 1 cut(s) 557
BbvI GCAGC 4 cut(s) 931, 1007, 1344, 1450
BccI CCATC 4 cut(s) 143, 204, 236, 1206
BceAI ACGGC 5 cut(s) 52, 206, 782, 851, 1462
BcgI CGANNNNNNTGC 2 cut(s) 538, 572
BciT130I CCWGG 1 cut(s) 812
BciVI GTATCC 1 cut(s) 1286
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 3 cut(s) 287, 967, 1035
BfaI CTAG 1 cut(s) 1136
BfmI CTRYAG 1 cut(s) 846
BfuI GTATCC 1 cut(s) 1286
BisI GCNGC 5 cut(s) 40, 945, 996, 1358, 1464
BlsI GCNGC 5 cut(s) 41, 946, 997, 1359, 1465
Bme1390I CCNGG 2 cut(s) 85, 812
Bme18I GGWCC 1 cut(s) 1414
BmeRI GACNNNNNGTC 1 cut(s) 1023
BmeT110I CYCGRG 1 cut(s) 434
BmgBI CACGTC 2 cut(s) 451, 986
BmgT120I GGNCC 3 cut(s) 131, 132, 1414
BmiI GGNNCC 3 cut(s) 133, 319, 1265
BmrFI CCNGG 2 cut(s) 85, 812
BmsI GCATC 3 cut(s) 70, 675, 792
BpmI CTGGAG 2 cut(s) 194, 833
BpuEI CTTGAG 1 cut(s) 809
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 2 cut(s) 625, 1015
BsaHI GRCGYC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 967
BsaWI WCCGGW 1 cut(s) 1416
BsaXI ACNNNNNCTCC 2 cut(s) 806, 836
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 4 cut(s) 11, 1181, 1227, 1391
Bse3DI GCAATG 2 cut(s) 654, 927
BseBI CCWGG 1 cut(s) 812
BseGI GGATG 4 cut(s) 85, 448, 1217, 1425
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMI GCAATG 2 cut(s) 654, 927
BseNI ACTGG 4 cut(s) 11, 1181, 1227, 1391
BseRI GAGGAG 1 cut(s) 1469
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 4 cut(s) 931, 1007, 1344, 1450
BseYI CCCAGC 2 cut(s) 128, 1433
BsgI GTGCAG 2 cut(s) 232, 669
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 4 cut(s) 133, 195, 306, 852
BsiEI CGRYCG 1 cut(s) 89
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 5 cut(s) 62, 85, 321, 778, 1417
BslFI GGGAC 2 cut(s) 1233, 1351
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 3 cut(s) 287, 967, 1035
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 2 cut(s) 1233, 1351
BsmI GAATGC 1 cut(s) 609
BsnI GGCC 4 cut(s) 133, 195, 306, 852
Bso31I GGTCTC 1 cut(s) 967
BsoBI CYCGRG 1 cut(s) 434
Bsp120I GGGCCC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 135, 1266
Bsp1407I TGTACA 2 cut(s) 592, 1095
Bsp143I GATC 3 cut(s) 356, 363, 1062
BspACI CCGC 8 cut(s) 39, 219, 414, 491, 795, 1209, 1268, 1335
BspANI GGCC 4 cut(s) 133, 195, 306, 852
BspLI GGNNCC 3 cut(s) 133, 319, 1265
BspPI GGATC 1 cut(s) 371
BspQI GCTCTTC 1 cut(s) 1350
BspTNI GGTCTC 1 cut(s) 967
BsrBI CCGCTC 1 cut(s) 1270
BsrDI GCAATG 2 cut(s) 654, 927
BsrFI RCCGGY 1 cut(s) 61
BsrGI TGTACA 2 cut(s) 592, 1095
BsrI ACTGG 4 cut(s) 11, 1181, 1227, 1391
BssAI RCCGGY 1 cut(s) 61
BssMI GATC 3 cut(s) 356, 363, 1062
BssNI GRCGYC 1 cut(s) 8
BssSI CACGAG 1 cut(s) 557
Bst2BI CACGAG 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 812
Bst4CI ACNGT 2 cut(s) 911, 1021
Bst6I CTCTTC 2 cut(s) 620, 1350
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 2 cut(s) 656, 1332
BstAUI TGTACA 2 cut(s) 592, 1095
BstBAI YACGTR 2 cut(s) 625, 1015
BstC8I GCNNGC 3 cut(s) 564, 652, 888
BstDEI CTNAG 1 cut(s) 857
BstF5I GGATG 4 cut(s) 85, 448, 1217, 1425
BstKTI GATC 3 cut(s) 359, 366, 1065
BstMAI GTCTC 3 cut(s) 287, 967, 1035
BstMBI GATC 3 cut(s) 356, 363, 1062
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 6 cut(s) 162, 317, 436, 461, 656, 1332
BstNI CCWGG 1 cut(s) 812
BstNSI RCATGY 2 cut(s) 337, 352
BstSCI CCNGG 2 cut(s) 83, 810
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 1 cut(s) 135
BstV1I GCAGC 4 cut(s) 931, 1007, 1344, 1450
BsuI GTATCC 1 cut(s) 1286
BsuRI GGCC 4 cut(s) 133, 195, 306, 852
BtgZI GCGATG 1 cut(s) 121
BtrI CACGTC 2 cut(s) 451, 986
BtsCI GGATG 4 cut(s) 85, 448, 1217, 1425
BtsI GCAGTG 2 cut(s) 17, 36
BtsIMutI CAGTG 3 cut(s) 17, 18, 36
Cac8I GCNNGC 3 cut(s) 564, 652, 888
CaiI CAGNNNCTG 2 cut(s) 1033, 1222
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 3 cut(s) 131, 132, 1414
CpoI CGGWCCG 1 cut(s) 1414
CseI GACGC 3 cut(s) 16, 304, 1162
Csp6I GTAC 4 cut(s) 593, 869, 1096, 1370
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CspI CGGWCCG 1 cut(s) 1414
CviAII CATG 9 cut(s) 50, 184, 268, 277, 334, 349, 360, 1054, 1327
CviQI GTAC 4 cut(s) 593, 869, 1096, 1370
DdeI CTNAG 1 cut(s) 857
DpnI GATC 3 cut(s) 358, 365, 1064
DpnII GATC 3 cut(s) 356, 363, 1062
DraIII CACNNNGTG 1 cut(s) 31
DriI GACNNNNNGTC 1 cut(s) 1023
EaeI YGGCCR 2 cut(s) 193, 304
Eam1104I CTCTTC 2 cut(s) 620, 1350
Eam1105I GACNNNNNGTC 1 cut(s) 1023
EarI CTCTTC 2 cut(s) 620, 1350
EciI GGCGGA 4 cut(s) 208, 403, 784, 1224
Eco24I GRGCYC 2 cut(s) 135, 1266
Eco31I GGTCTC 1 cut(s) 967
Eco47I GGWCC 1 cut(s) 1414
Eco57I CTGAAG 2 cut(s) 58, 521
Eco88I CYCGRG 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 810
EcoT38I GRGCYC 2 cut(s) 135, 1266
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 9 cut(s) 53, 187, 271, 280, 337, 352, 363, 1057, 1330
FaqI GGGAC 2 cut(s) 1233, 1351
FatI CATG 9 cut(s) 49, 183, 267, 276, 333, 348, 359, 1053, 1326
FauI CCCGC 2 cut(s) 1275, 1328
FauNDI CATATG 1 cut(s) 764
FblI GTMKAC 1 cut(s) 1011
Fnu4HI GCNGC 5 cut(s) 40, 945, 996, 1358, 1464
FokI GGATG 4 cut(s) 92, 455, 1224, 1432
FriOI GRGCYC 2 cut(s) 135, 1266
Fsp4HI GCNGC 5 cut(s) 40, 945, 996, 1358, 1464
FspBI CTAG 1 cut(s) 1136
GluI GCNGC 5 cut(s) 40, 945, 996, 1358, 1464
GsaI CCCAGC 2 cut(s) 132, 1437
GsuI CTGGAG 2 cut(s) 194, 833
HaeIII GGCC 4 cut(s) 133, 195, 306, 852
HapII CCGG 5 cut(s) 62, 85, 321, 778, 1417
HgaI GACGC 3 cut(s) 16, 304, 1162
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 9 cut(s) 53, 187, 271, 280, 337, 352, 363, 1057, 1330
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HindIII AAGCTT 2 cut(s) 309, 428
HinfI GANTC 5 cut(s) 791, 832, 881, 936, 1044
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 5 cut(s) 62, 85, 321, 778, 1417
HphI GGTGA 4 cut(s) 16, 76, 367, 466
Hpy166II GTNNAC 8 cut(s) 34, 331, 346, 375, 732, 1012, 1098, 1237
Hpy188I TCNGA 5 cut(s) 203, 317, 1043, 1151, 1205
Hpy188III TCNNGA 1 cut(s) 1066
Hpy8I GTNNAC 8 cut(s) 34, 331, 346, 375, 732, 1012, 1098, 1237
Hpy99I CGWCG 1 cut(s) 1454
HpyAV CCTTC 7 cut(s) 151, 262, 520, 545, 719, 1289, 1303
HpyCH4III ACNGT 2 cut(s) 911, 1021
HpyCH4IV ACGT 5 cut(s) 327, 450, 624, 985, 1014
HpyCH4V TGCA 9 cut(s) 156, 249, 352, 486, 650, 659, 720, 1053, 1117
HpyF10VI GCNNNNNNNGC 6 cut(s) 162, 317, 436, 461, 656, 1332
HpyF3I CTNAG 1 cut(s) 857
HpySE526I ACGT 5 cut(s) 327, 450, 624, 985, 1014
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 9 cut(s) 53, 187, 271, 280, 337, 352, 363, 1057, 1330
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 356, 363, 1062
LguI GCTCTTC 1 cut(s) 1350
LmnI GCTCC 2 cut(s) 317, 1269
Lsp1109I GCAGC 4 cut(s) 931, 1007, 1344, 1450
LweI GCATC 3 cut(s) 70, 675, 792
MaeI CTAG 1 cut(s) 1136
MaeII ACGT 5 cut(s) 327, 450, 624, 985, 1014
MaeIII GTNAC 4 cut(s) 4, 1015, 1153, 1247
MalI GATC 3 cut(s) 358, 365, 1064
MbiI CCGCTC 1 cut(s) 1270
MboI GATC 3 cut(s) 356, 363, 1062
MboII GAAGA 5 cut(s) 400, 607, 1136, 1337, 1435
MhlI GDGCHC 2 cut(s) 135, 1266
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 7 cut(s) 123, 597, 681, 901, 1112, 1140, 1314
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 3 cut(s) 800, 826, 875
MmeI TCCRAC 2 cut(s) 990, 1201
MnlI CCTC 8 cut(s) 60, 218, 401, 1102, 1199, 1316, 1390, 1447
Mox20I TGGCCA 1 cut(s) 306
MroXI GAANNNNTTC 1 cut(s) 607
MscI TGGCCA 1 cut(s) 306
MseI TTAA 2 cut(s) 330, 899
MslI CAYNNNNRTG 2 cut(s) 623, 1058
Msp20I TGGCCA 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 381
MspI CCGG 5 cut(s) 62, 85, 321, 778, 1417
MspR9I CCNGG 2 cut(s) 85, 812
Mva1269I GAATGC 1 cut(s) 609
MvaI CCWGG 1 cut(s) 812
MwoI GCNNNNNNNGC 6 cut(s) 162, 317, 436, 461, 656, 1332
NciI CCSGG 1 cut(s) 85
NdeI CATATG 1 cut(s) 764
NdeII GATC 3 cut(s) 356, 363, 1062
NlaIII CATG 9 cut(s) 53, 187, 271, 280, 337, 352, 363, 1057, 1330
NlaIV GGNNCC 3 cut(s) 133, 319, 1265
NmeAIII GCCGAG 1 cut(s) 318
NmuCI GTSAC 3 cut(s) 4, 1015, 1153
NspI RCATGY 2 cut(s) 337, 352
PciSI GCTCTTC 1 cut(s) 1350
PcsI WCGNNNNNNNCGW 1 cut(s) 749
PctI GAATGC 1 cut(s) 609
PdmI GAANNNNTTC 1 cut(s) 607
PfeI GAWTC 2 cut(s) 936, 1044
PkrI GCNGC 5 cut(s) 41, 946, 997, 1359, 1465
PleI GAGTC 3 cut(s) 799, 826, 875
PpsI GAGTC 3 cut(s) 799, 826, 875
Ppu21I YACGTR 2 cut(s) 625, 1015
Psp6I CCWGG 1 cut(s) 810
PspFI CCCAGC 2 cut(s) 128, 1433
PspGI CCWGG 1 cut(s) 810
PspN4I GGNNCC 3 cut(s) 133, 319, 1265
PspOMI GGGCCC 1 cut(s) 131
PspPI GGNCC 3 cut(s) 131, 132, 1414
PstNI CAGNNNCTG 2 cut(s) 1033, 1222
PvuII CAGCTG 1 cut(s) 381
RsaI GTAC 4 cut(s) 594, 870, 1097, 1371
RsaNI GTAC 4 cut(s) 593, 869, 1096, 1370
RseI CAYNNNNRTG 2 cut(s) 623, 1058
Rsr2I CGGWCCG 1 cut(s) 1414
RsrII CGGWCCG 1 cut(s) 1414
SapI GCTCTTC 1 cut(s) 1350
SaqAI TTAA 2 cut(s) 330, 899
SatI GCNGC 5 cut(s) 40, 945, 996, 1358, 1464
Sau3AI GATC 3 cut(s) 356, 363, 1062
Sau96I GGNCC 3 cut(s) 131, 132, 1414
SchI GAGTC 3 cut(s) 800, 826, 875
ScrFI CCNGG 2 cut(s) 85, 812
SduI GDGCHC 2 cut(s) 135, 1266
SfaNI GCATC 3 cut(s) 70, 675, 792
SfcI CTRYAG 1 cut(s) 846
SinI GGWCC 1 cut(s) 1414
SmiMI CAYNNNNRTG 2 cut(s) 623, 1058
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
Sse9I AATT 7 cut(s) 123, 597, 681, 901, 1112, 1140, 1314
SsiI CCGC 8 cut(s) 39, 219, 414, 491, 795, 1209, 1268, 1335
SspI AATATT 2 cut(s) 700, 1004
SspMI CTAG 1 cut(s) 1136
StyD4I CCNGG 2 cut(s) 83, 810
TaaI ACNGT 2 cut(s) 911, 1021
TaiI ACGT 5 cut(s) 330, 453, 627, 988, 1017
TaqI TCGA 1 cut(s) 630
TasI AATT 7 cut(s) 123, 597, 681, 901, 1112, 1140, 1314
TatI WGTACW 3 cut(s) 592, 1095, 1369
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 2 cut(s) 936, 1044
Tru1I TTAA 2 cut(s) 330, 899
Tru9I TTAA 2 cut(s) 330, 899
TscAI CASTG 3 cut(s) 18, 24, 36
TseFI GTSAC 3 cut(s) 4, 1015, 1153
TseI GCWGC 4 cut(s) 944, 995, 1357, 1463
Tsp45I GTSAC 3 cut(s) 4, 1015, 1153
TspDTI ATGAA 6 cut(s) 117, 256, 607, 725, 919, 1436
TspGWI ACGGA 2 cut(s) 229, 1427
TspRI CASTG 3 cut(s) 18, 24, 36
VpaK11BI GGWCC 1 cut(s) 1414
XapI RAATTY 2 cut(s) 901, 1314
XceI RCATGY 2 cut(s) 337, 352
XmiI GTMKAC 1 cut(s) 1011
XmnI GAANNNNTTC 1 cut(s) 607
XspI CTAG 1 cut(s) 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.