Rorug01G0406100

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000970
Physical Location & Seq
Forward (+)
51285723 .. 51288906
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0406100.1

Sequence Viewer

Length: 1317 bp
ATGGGGAAAGGTCCGTTCTCGTTGAAGCGTAGCAGCAGCAGCCGGCGGCAGCGGCCGAAGAAGTTTATCGGCCCTCCACCTTCTCCTTTCCCTTCGCCTCCTCAGCCTCGGCCCATGGGATCCGCCCCCTCCTCGCCCTCGAACAACAACAGTTTGGCTGTCAAGGCCAAGAAGAAGGCCGGCGGAGGAGCCAGGCTCTGGATGCGATTCGATCGCTGCGGCGGTTCCGAGCTTGTCGAGTGTGACAAGAATGCTATCATCCGACGCGCCGCAATTCCCCCTCGTGACTTGAGAATTCTCGGCCCTGTTTTCTCCCATTCCTCCAGCATCCTCGCCAGGGAGAAGGCCATGGTGGTGAATTTGGAGTTTATCAAGGCTATAGTTACCGCCGAAGAGGTTCTGTTGCTTGATCCTCTTCGGCAGGAGGTTCTTCCATTTGCGGATCAACTAAGGCAACAGCTTCCTCAATCAAGGGTTGATGAACAAGATAATCAACTGGATGCGTCAACAGCAAGGCAATGGCTACCTGTTCCCGAGGCCGTTGAAGGTTTGCAATGTGATCTCCCATTTGAGTTTCAGGTTCTCGAGATTGCTCTAGAAGTTGTCTGTACGTACTTGGATTCCAGTGTGGCAGACCTTGAGATGGAAGCCTACCCTGTGCTCGATGAACTCGCTAGAAATGTCAGCACCAAGAATCTTGAACTTGTTCGCAGTTTGAAAAGCAATCTCACCCGTTTGCTTGCACGAGTACAGAAGGTGAGGGATGAAATTGAACATTTGTTGGATGACAATGAAGACATGGCACATTTATATTTGACAAGAAAGTGGATTCAGAATCAACAATCTGAGGCCTTGTTGGCACGTTCTGGCTCAAATAGAAGCAGCCCTACTGCACCCAATCTCCGTCGGCTAGGCTCTAATAGAAGCAGTAGTTTGGTAACTAGCAACAACATGGGTGAAGATGATGTAGAGGATCTAGAAATGTTGCTTGAGGCTTATTTTATGCAACTTGATGGAACTCGTAACAAGATATTGTCTGTACGGGAGTATATAGATGACACAGAGGATTATGTCAACATTCAACTTGACAACCAGCGAAACGAACTCATTCAGCTCCAATTAACATTGACCATTGCATCATTTGCTATAGCCGTAGAGACCTTGATCGCTGGAATATTTGGTATGAACATTCCCTGTGTATTATACGACAAGAAGGGGATATTTGAGTTCTTTGTTGGAGGTGTCACTGCATTTTCGGTATTGCTTTTCTTCCTTGTTTTTGGGTATGCAAGGTGGAAGAAGCTGCTTGGTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

438

Amino Acids

49.08

Weight (kDa)

5.89

Isoelectric Point (pI)

61.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 80 - 280 1.6e-24 Magnesium transporter MRS2-like
CorA PF01544 232 - 427 5.3e-07 CorA-like Mg2+ transporter protein
MRS2-like PF22099 314 - 366 4.1e-07 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 198
AccII CGCG 1 cut(s) 267
AciI CCGC 9 cut(s) 46, 52, 123, 183, 219, 222, 270, 387, 440
AclWI GGATC 5 cut(s) 114, 127, 404, 450, 981
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 2 cut(s) 294, 358
AfaI GTAC 4 cut(s) 610, 614, 750, 1041
AfiI CCNNNNNNNGG 3 cut(s) 198, 337, 643
AgsI TTSAA 6 cut(s) 25, 545, 701, 718, 773, 1082
AjnI CCWGG 2 cut(s) 191, 335
AluBI AGCT 4 cut(s) 232, 460, 1114, 1303
AluI AGCT 4 cut(s) 232, 460, 1114, 1303
Alw21I GWGCWC 1 cut(s) 663
Alw26I GTCTC 1 cut(s) 1151
AlwI GGATC 5 cut(s) 114, 127, 404, 450, 981
AlwNI CAGNNNCTG 1 cut(s) 198
Ama87I CYCGRG 2 cut(s) 533, 584
AoxI GGCC 9 cut(s) 53, 70, 110, 165, 177, 301, 345, 537, 849
ApeKI GCWGC 7 cut(s) 33, 36, 39, 49, 216, 882, 1303
ApoI RAATTY 2 cut(s) 294, 358
Asp700I GAANNNNTTC 3 cut(s) 396, 705, 1107
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 4 cut(s) 11, 71, 111, 302
AsuHPI GGTGA 4 cut(s) 367, 721, 769, 968
AvaI CYCGRG 2 cut(s) 533, 584
AvaII GGWCC 1 cut(s) 11
BamHI GGATCC 1 cut(s) 119
BarI GAAGNNNNNNTAC 2 cut(s) 871, 903
BauI CACGAG 2 cut(s) 282, 744
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 7 cut(s) 45, 48, 51, 61, 203, 894, 1290
BccI CCATC 2 cut(s) 637, 1007
BceAI ACGGC 2 cut(s) 524, 1136
BciT130I CCWGG 2 cut(s) 193, 337
BcoDI GTCTC 1 cut(s) 1151
BfaI CTAG 5 cut(s) 596, 675, 911, 942, 977
BfmI CTRYAG 2 cut(s) 378, 1146
BglI GCCNNNNNGGC 1 cut(s) 857
Bme1390I CCNGG 2 cut(s) 193, 337
Bme18I GGWCC 1 cut(s) 11
BmeT110I CYCGRG 2 cut(s) 533, 584
BmgT120I GGNCC 4 cut(s) 11, 71, 111, 302
BmiI GGNNCC 3 cut(s) 121, 190, 226
BmrFI CCNGG 2 cut(s) 193, 337
BmsI GCATC 4 cut(s) 192, 336, 490, 1145
BpiI GAAGAC 1 cut(s) 801
BplI GAGNNNNNCTC 2 cut(s) 180, 212
BpmI CTGGAG 1 cut(s) 307
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 3 cut(s) 310, 659, 1010
BsaAI YACGTR 1 cut(s) 612
BsaI GGTCTC 1 cut(s) 1151
BsaJI CCNNGG 5 cut(s) 107, 114, 336, 348, 534
BsaXI ACNNNNNCTCC 2 cut(s) 885, 915
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 337, 643
Bse118I RCCGGY 2 cut(s) 42, 179
Bse1I ACTGG 2 cut(s) 501, 624
Bse3DI GCAATG 3 cut(s) 524, 560, 1131
BseBI CCWGG 2 cut(s) 193, 337
BseDI CCNNGG 5 cut(s) 107, 114, 336, 348, 534
BseGI GGATG 6 cut(s) 207, 258, 327, 505, 769, 790
BseLI CCNNNNNNNGG 3 cut(s) 198, 337, 643
BseMI GCAATG 3 cut(s) 524, 560, 1131
BseMII CTCAG 2 cut(s) 116, 837
BseNI ACTGG 2 cut(s) 501, 624
BseRI GAGGAG 3 cut(s) 90, 121, 201
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 7 cut(s) 45, 48, 51, 61, 203, 894, 1290
BsgI GTGCAG 1 cut(s) 876
Bsh1236I CGCG 1 cut(s) 267
Bsh1285I CGRYCG 2 cut(s) 56, 214
BshFI GGCC 9 cut(s) 55, 72, 112, 167, 179, 303, 347, 539, 851
BsiEI CGRYCG 2 cut(s) 56, 214
BsiHKAI GWGCWC 1 cut(s) 663
BsiHKCI CYCGRG 2 cut(s) 533, 584
BsiSI CCGG 2 cut(s) 43, 180
BslI CCNNNNNNNGG 3 cut(s) 198, 337, 643
BsmAI GTCTC 1 cut(s) 1151
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 9 cut(s) 55, 72, 112, 167, 179, 303, 347, 539, 851
Bso31I GGTCTC 1 cut(s) 1151
BsoBI CYCGRG 2 cut(s) 533, 584
Bsp1286I GDGCHC 1 cut(s) 663
Bsp143I GATC 7 cut(s) 119, 211, 409, 442, 559, 973, 1164
Bsp19I CCATGG 2 cut(s) 114, 348
BspACI CCGC 9 cut(s) 46, 52, 123, 183, 219, 222, 270, 387, 440
BspANI GGCC 9 cut(s) 55, 72, 112, 167, 179, 303, 347, 539, 851
BspCNI CTCAG 2 cut(s) 115, 838
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 3 cut(s) 121, 190, 226
BspPI GGATC 5 cut(s) 114, 127, 404, 450, 981
BspTNI GGTCTC 1 cut(s) 1151
BsrDI GCAATG 3 cut(s) 524, 560, 1131
BsrFI RCCGGY 2 cut(s) 42, 179
BsrI ACTGG 2 cut(s) 501, 624
BssAI RCCGGY 2 cut(s) 42, 179
BssECI CCNNGG 5 cut(s) 107, 114, 336, 348, 534
BssMI GATC 7 cut(s) 119, 211, 409, 442, 559, 973, 1164
BssSI CACGAG 2 cut(s) 282, 744
BssT1I CCWWGG 2 cut(s) 114, 348
Bst2BI CACGAG 2 cut(s) 282, 744
Bst2UI CCWGG 2 cut(s) 193, 337
Bst4CI ACNGT 1 cut(s) 152
Bst6I CTCTTC 2 cut(s) 387, 420
BstAPI GCANNNNNTGC 1 cut(s) 1142
BstBAI YACGTR 1 cut(s) 612
BstC8I GCNNGC 3 cut(s) 44, 181, 741
BstDEI CTNAG 3 cut(s) 102, 449, 846
BstDSI CCRYGG 2 cut(s) 114, 348
BstF5I GGATG 6 cut(s) 207, 258, 327, 505, 769, 790
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 7 cut(s) 122, 214, 412, 445, 562, 976, 1167
BstMAI GTCTC 1 cut(s) 1151
BstMBI GATC 7 cut(s) 119, 211, 409, 442, 559, 973, 1164
BstMCI CGRYCG 2 cut(s) 56, 214
BstMWI GCNNNNNNNGC 8 cut(s) 39, 52, 103, 164, 202, 509, 857, 1142
BstNI CCWGG 2 cut(s) 193, 337
BstSCI CCNGG 2 cut(s) 191, 335
BstSFI CTRYAG 2 cut(s) 378, 1146
BstSNI TACGTA 1 cut(s) 612
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 7 cut(s) 45, 48, 51, 61, 203, 894, 1290
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 2 cut(s) 119, 973
BstYI RGATCY 2 cut(s) 119, 973
BstZI CGGCCG 1 cut(s) 53
BsuRI GGCC 9 cut(s) 55, 72, 112, 167, 179, 303, 347, 539, 851
BtgI CCRYGG 2 cut(s) 114, 348
BtsCI GGATG 6 cut(s) 207, 258, 327, 505, 769, 790
BtsI GCAGTG 1 cut(s) 1245
BtsIMutI CAGTG 2 cut(s) 631, 1245
Cac8I GCNNGC 3 cut(s) 44, 181, 741
CaiI CAGNNNCTG 1 cut(s) 198
CfoI GCGC 1 cut(s) 269
Cfr10I RCCGGY 2 cut(s) 42, 179
Cfr13I GGNCC 4 cut(s) 11, 71, 111, 302
CseI GACGC 2 cut(s) 273, 492
Csp6I GTAC 4 cut(s) 609, 613, 749, 1040
CviAII CATG 4 cut(s) 115, 349, 799, 952
CviQI GTAC 4 cut(s) 609, 613, 749, 1040
DdeI CTNAG 3 cut(s) 102, 449, 846
DpnI GATC 7 cut(s) 121, 213, 411, 444, 561, 975, 1166
DpnII GATC 7 cut(s) 119, 211, 409, 442, 559, 973, 1164
EaeI YGGCCR 1 cut(s) 53
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 2 cut(s) 387, 420
EarI CTCTTC 2 cut(s) 387, 420
EciI GGCGGA 2 cut(s) 112, 198
EclXI CGGCCG 1 cut(s) 53
Eco105I TACGTA 1 cut(s) 612
Eco130I CCWWGG 2 cut(s) 114, 348
Eco147I AGGCCT 1 cut(s) 851
Eco31I GGTCTC 1 cut(s) 1151
Eco47I GGWCC 1 cut(s) 11
Eco52I CGGCCG 1 cut(s) 53
Eco88I CYCGRG 2 cut(s) 533, 584
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 2 cut(s) 191, 335
EcoT14I CCWWGG 2 cut(s) 114, 348
ErhI CCWWGG 2 cut(s) 114, 348
FaeI CATG 4 cut(s) 118, 352, 802, 955
FatI CATG 4 cut(s) 114, 348, 798, 951
FokI GGATG 6 cut(s) 214, 245, 314, 512, 776, 797
FspBI CTAG 5 cut(s) 596, 675, 911, 942, 977
GlaI GCGC 1 cut(s) 268
GsuI CTGGAG 1 cut(s) 307
HaeIII GGCC 9 cut(s) 55, 72, 112, 167, 179, 303, 347, 539, 851
HapII CCGG 2 cut(s) 43, 180
HgaI GACGC 2 cut(s) 273, 492
HhaI GCGC 1 cut(s) 269
Hin1II CATG 4 cut(s) 118, 352, 802, 955
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HincII GTYRAC 2 cut(s) 507, 1075
HindII GTYRAC 2 cut(s) 507, 1075
HinfI GANTC 5 cut(s) 207, 620, 694, 829, 835
HpaII CCGG 2 cut(s) 43, 180
HphI GGTGA 4 cut(s) 367, 721, 769, 968
Hpy166II GTNNAC 2 cut(s) 507, 1075
Hpy188I TCNGA 4 cut(s) 229, 263, 834, 847
Hpy188III TCNNGA 9 cut(s) 199, 284, 533, 584, 586, 596, 698, 977, 1314
Hpy8I GTNNAC 2 cut(s) 507, 1075
Hpy99I CGWCG 2 cut(s) 267, 909
HpyAV CCTTC 7 cut(s) 90, 102, 169, 337, 539, 748, 1207
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4IV ACGT 2 cut(s) 611, 862
HpyCH4V TGCA 7 cut(s) 553, 743, 893, 1006, 1136, 1250, 1289
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 52, 103, 164, 202, 509, 857, 1142
HpyF3I CTNAG 3 cut(s) 102, 449, 846
HpySE526I ACGT 2 cut(s) 611, 862
Hsp92II CATG 4 cut(s) 118, 352, 802, 955
HspAI GCGC 1 cut(s) 267
KroI GCCGGC 2 cut(s) 42, 179
KroNI GCCGGC 2 cut(s) 44, 181
Kzo9I GATC 7 cut(s) 119, 211, 409, 442, 559, 973, 1164
LmnI GCTCC 2 cut(s) 188, 1119
Lsp1109I GCAGC 7 cut(s) 45, 48, 51, 61, 203, 894, 1290
LweI GCATC 4 cut(s) 192, 336, 490, 1145
MaeI CTAG 5 cut(s) 596, 675, 911, 942, 977
MaeII ACGT 2 cut(s) 611, 862
MaeIII GTNAC 6 cut(s) 242, 284, 382, 937, 1022, 1243
MalI GATC 7 cut(s) 121, 213, 411, 444, 561, 975, 1166
MboI GATC 7 cut(s) 119, 211, 409, 442, 559, 973, 1164
MboII GAAGA 9 cut(s) 70, 184, 404, 407, 422, 806, 971, 1261, 1309
MflI RGATCY 2 cut(s) 119, 973
MhlI GDGCHC 1 cut(s) 663
MluCI AATT 5 cut(s) 273, 294, 358, 768, 1118
MmeI TCCRAC 3 cut(s) 286, 762, 1216
MroNI GCCGGC 2 cut(s) 42, 179
MroXI GAANNNNTTC 3 cut(s) 396, 705, 1107
MseI TTAA 1 cut(s) 1121
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 52
MspI CCGG 2 cut(s) 43, 180
MspR9I CCNGG 2 cut(s) 193, 337
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 2 cut(s) 193, 337
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 8 cut(s) 39, 52, 103, 164, 202, 509, 857, 1142
NaeI GCCGGC 2 cut(s) 44, 181
NcoI CCATGG 2 cut(s) 114, 348
NdeII GATC 7 cut(s) 119, 211, 409, 442, 559, 973, 1164
NgoMIV GCCGGC 2 cut(s) 42, 179
NlaIII CATG 4 cut(s) 118, 352, 802, 955
NlaIV GGNNCC 3 cut(s) 121, 190, 226
NmeAIII GCCGAG 2 cut(s) 88, 279
NmuCI GTSAC 3 cut(s) 242, 284, 1243
PaeR7I CTCGAG 1 cut(s) 584
PceI AGGCCT 1 cut(s) 851
PctI GAATGC 1 cut(s) 256
PdiI GCCGGC 2 cut(s) 44, 181
PdmI GAANNNNTTC 3 cut(s) 396, 705, 1107
PfeI GAWTC 5 cut(s) 207, 620, 694, 829, 835
PflMI CCANNNNNTGG 1 cut(s) 198
Ple19I CGATCG 1 cut(s) 214
Ppu21I YACGTR 1 cut(s) 612
Psp6I CCWGG 2 cut(s) 191, 335
PspGI CCWGG 2 cut(s) 191, 335
PspN4I GGNNCC 3 cut(s) 121, 190, 226
PspPI GGNCC 4 cut(s) 11, 71, 111, 302
PstNI CAGNNNCTG 1 cut(s) 198
PsuI RGATCY 2 cut(s) 119, 973
PvuI CGATCG 1 cut(s) 214
RsaI GTAC 4 cut(s) 610, 614, 750, 1041
RsaNI GTAC 4 cut(s) 609, 613, 749, 1040
RseI CAYNNNNRTG 1 cut(s) 353
SaqAI TTAA 1 cut(s) 1121
Sau3AI GATC 7 cut(s) 119, 211, 409, 442, 559, 973, 1164
Sau96I GGNCC 4 cut(s) 11, 71, 111, 302
ScrFI CCNGG 2 cut(s) 193, 337
SduI GDGCHC 1 cut(s) 663
SfaNI GCATC 4 cut(s) 192, 336, 490, 1145
SfcI CTRYAG 2 cut(s) 378, 1146
Sfr274I CTCGAG 1 cut(s) 584
SinI GGWCC 1 cut(s) 11
SlaI CTCGAG 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 4 cut(s) 289, 584, 638, 989
SmoI CTYRAG 4 cut(s) 289, 584, 638, 989
SnaBI TACGTA 1 cut(s) 612
Sse9I AATT 5 cut(s) 273, 294, 358, 768, 1118
SseBI AGGCCT 1 cut(s) 851
SsiI CCGC 9 cut(s) 46, 52, 123, 183, 219, 222, 270, 387, 440
SspI AATATT 1 cut(s) 1176
SspMI CTAG 5 cut(s) 596, 675, 911, 942, 977
StuI AGGCCT 1 cut(s) 851
StyD4I CCNGG 2 cut(s) 191, 335
StyI CCWWGG 2 cut(s) 114, 348
TaaI ACNGT 1 cut(s) 152
TaiI ACGT 2 cut(s) 614, 865
TaqI TCGA 5 cut(s) 140, 210, 237, 585, 663
TasI AATT 5 cut(s) 273, 294, 358, 768, 1118
TatI WGTACW 1 cut(s) 748
TauI GCSGC 4 cut(s) 49, 55, 222, 272
TfiI GAWTC 5 cut(s) 207, 620, 694, 829, 835
Tru1I TTAA 1 cut(s) 1121
Tru9I TTAA 1 cut(s) 1121
TscAI CASTG 2 cut(s) 631, 1252
TseFI GTSAC 3 cut(s) 242, 284, 1243
TseI GCWGC 7 cut(s) 33, 36, 39, 49, 216, 882, 1303
Tsp45I GTSAC 3 cut(s) 242, 284, 1243
TspDTI ATGAA 5 cut(s) 495, 681, 780, 807, 1199
TspGWI ACGGA 1 cut(s) 893
TspRI CASTG 2 cut(s) 631, 1252
Van91I CCANNNNNTGG 1 cut(s) 198
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 2 cut(s) 294, 358
XbaI TCTAGA 2 cut(s) 595, 976
XhoI CTCGAG 1 cut(s) 584
XmnI GAANNNNTTC 3 cut(s) 396, 705, 1107
XspI CTAG 5 cut(s) 596, 675, 911, 942, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.