pycom01g21660

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000970
Physical Location & Seq
Reverse (-)
19602634 .. 19605690
3057 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21660.6

Sequence Viewer

Length: 1065 bp
ATGCATGGGTTAGGACTGTTAGCTTGGGATGGGGAGTGCACTGGAGACCCTGTGTGGACAAGTGACATAGCGAATAAAGCCGGAAAATTCACACTCCAAATAATCCCCCAATTGTTCTCACTTGCCATAAATTTCCCAGCCCAAAAGTTCCTCCAGGCTCAGAGGAAGGTGAAGGTGCTGGCATGGATTGCAGTGTTGGCCCTGGTCATACACATTGGAATGCTAGCGCTCTTCATGTATGTGTTTGATTGGGGCACATTGGGGGCAGCTGTGTCATTTAACCTCACAAGATGGGGGATTTCAATTGCACAAGCTGTGTATATTATGGGTTGGTGCAAGGAGGGTTGGACTGGATTTTCATGGCTGGCATTCAATGAGATTTGGGCATTTGTGAGACTTTCTCTTGCCTCAGCAGTCATGCTCTGCCTAGAAATTTGGTACATGATGAGTATGCTCATTCTCACGGGCGGTCTTTCTGATGCAGTTATAGCCGTTGGTTCTCTTTCAATTTGCATGAACGTCAACGGGTGGGAAACAATGTTGTTCGTTGGAATAAATGCTGCAATAAGTGTTCGTGTCTCCAATGAGCTTGGATTAAGACGTCCTAGAGCAGTCAAATACGCCGTTTATGTGACAGTATCTCAATCTCTCTTGCTTGGTCTTCTTTTCATGATTATTATTTTAATAACCAAAGACGATTTTGCCGTAATTTTCACAAGTGACAAGGAGCTGCAACAAGCGGTTGCTAAGCTTGCATACCTTCTTGGTATAACTATGGTGCTCAACAGTATCCAACCAGTAATATCAGGTATCTATTTGACAAGCTACTATCATGATATGATGCCATGTTTTGATCTTGCTCTTGAAGGTGTTGCAATTGGAGGTGGATGGCAGACAATGGTTGCTTATATAAACTTGGGTTGTTACTACATTTTTGGACTTCCTCTTGGATACGTTCTTTGCCATGTTGCAAATTTAGGAGTCACGGTGGAGCAAGCAACAATTCGTGTTCGGAAGTGGGGAGGGCAAGACATTAAAACTGCCAACGGAGCTGAAAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

355

Amino Acids

38.92

Weight (kDa)

6.88

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 604
AciI CCGC 2 cut(s) 468, 740
AcsI RAATTY 4 cut(s) 86, 130, 432, 973
AcyI GRCGYC 1 cut(s) 601
AfaI GTAC 2 cut(s) 440, 1060
AfeI AGCGCT 1 cut(s) 228
AgsI TTSAA 4 cut(s) 303, 373, 507, 866
AjnI CCWGG 2 cut(s) 153, 201
AluBI AGCT 8 cut(s) 23, 269, 314, 589, 730, 751, 825, 1052
AluI AGCT 8 cut(s) 23, 269, 314, 589, 730, 751, 825, 1052
Alw21I GWGCWC 2 cut(s) 41, 783
Alw26I GTCTC 3 cut(s) 39, 388, 583
Alw44I GTGCAC 1 cut(s) 37
Aor51HI AGCGCT 1 cut(s) 228
AoxI GGCC 1 cut(s) 198
ApaLI GTGCAC 1 cut(s) 37
ApeKI GCWGC 3 cut(s) 266, 560, 730
ApoI RAATTY 4 cut(s) 86, 130, 432, 973
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 1 cut(s) 181
AsuNHI GCTAGC 1 cut(s) 223
BaeGI GKGCMC 2 cut(s) 41, 257
BbsI GAAGAC 1 cut(s) 653
Bbv12I GWGCWC 2 cut(s) 41, 783
BbvCI CCTCAGC 1 cut(s) 409
BbvI GCAGC 3 cut(s) 278, 547, 717
BccI CCATC 3 cut(s) 23, 285, 882
BceAI ACGGC 3 cut(s) 476, 608, 689
BciT130I CCWGG 2 cut(s) 155, 203
BciVI GTATCC 2 cut(s) 800, 944
BcoDI GTCTC 3 cut(s) 39, 388, 583
BfaI CTAG 3 cut(s) 224, 428, 606
BfoI RGCGCY 1 cut(s) 230
BfuI GTATCC 2 cut(s) 800, 944
BisI GCNGC 3 cut(s) 267, 561, 731
BlpI GCTNAGC 1 cut(s) 747
BlsI GCNGC 3 cut(s) 268, 562, 732
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 2 cut(s) 155, 203
BmgT120I GGNCC 1 cut(s) 199
BmrFI CCNGG 2 cut(s) 155, 203
BmsI GCATC 2 cut(s) 469, 831
BmtI GCTAGC 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 653
BplI GAGNNNNNCTC 2 cut(s) 385, 417
BpmI CTGGAG 2 cut(s) 63, 137
Bpu10I CCTNAGC 1 cut(s) 409
Bpu1102I GCTNAGC 1 cut(s) 747
BsaHI GRCGYC 1 cut(s) 601
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 201
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 972, 1002
Bse1I ACTGG 3 cut(s) 46, 355, 797
BseBI CCWGG 2 cut(s) 155, 203
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 2 cut(s) 34, 893
BseMII CTCAG 2 cut(s) 173, 423
BseNI ACTGG 3 cut(s) 46, 355, 797
BseSI GKGCMC 2 cut(s) 41, 257
BseXI GCAGC 3 cut(s) 278, 547, 717
BseYI CCCAGC 1 cut(s) 136
BshFI GGCC 1 cut(s) 200
BsiHKAI GWGCWC 2 cut(s) 41, 783
BsiSI CCGG 1 cut(s) 81
BsmAI GTCTC 3 cut(s) 39, 388, 583
BsmI GAATGC 2 cut(s) 225, 368
BsnI GGCC 1 cut(s) 200
Bso31I GGTCTC 1 cut(s) 39
Bsp1286I GDGCHC 3 cut(s) 41, 257, 783
Bsp143I GATC 1 cut(s) 853
Bsp1720I GCTNAGC 1 cut(s) 747
BspACI CCGC 2 cut(s) 468, 740
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 2 cut(s) 172, 422
BspHI TCATGA 2 cut(s) 669, 832
BspOI GCTAGC 1 cut(s) 227
BspQI GCTCTTC 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 39
BsrI ACTGG 3 cut(s) 46, 355, 797
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 1 cut(s) 853
BssNI GRCGYC 1 cut(s) 601
Bst2UI CCWGG 2 cut(s) 155, 203
Bst4CI ACNGT 4 cut(s) 18, 637, 788, 988
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 601
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 5 cut(s) 180, 225, 366, 753, 996
BstDEI CTNAG 3 cut(s) 159, 409, 747
BstF5I GGATG 2 cut(s) 34, 893
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 1 cut(s) 856
BstMAI GTCTC 3 cut(s) 39, 388, 583
BstMBI GATC 1 cut(s) 853
BstMWI GCNNNNNNNGC 6 cut(s) 77, 188, 197, 488, 752, 1049
BstNI CCWGG 2 cut(s) 155, 203
BstSCI CCNGG 2 cut(s) 153, 201
BstSLI GKGCMC 2 cut(s) 41, 257
BstV1I GCAGC 3 cut(s) 278, 547, 717
BstV2I GAAGAC 1 cut(s) 653
BsuI GTATCC 2 cut(s) 800, 944
BsuRI GGCC 1 cut(s) 200
BtsCI GGATG 2 cut(s) 34, 893
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 39, 198
Cac8I GCNNGC 5 cut(s) 180, 225, 366, 753, 996
CciI TCATGA 2 cut(s) 669, 832
CfoI GCGC 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 2 cut(s) 439, 1059
CviQI GTAC 2 cut(s) 439, 1059
DdeI CTNAG 3 cut(s) 159, 409, 747
DpnI GATC 1 cut(s) 855
DpnII GATC 1 cut(s) 853
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Eco31I GGTCTC 1 cut(s) 39
Eco47III AGCGCT 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 153, 201
EcoT22I ATGCAT 1 cut(s) 6
Fnu4HI GCNGC 3 cut(s) 267, 561, 731
FokI GGATG 2 cut(s) 41, 900
Fsp4HI GCNGC 3 cut(s) 267, 561, 731
FspBI CTAG 3 cut(s) 224, 428, 606
GlaI GCGC 1 cut(s) 228
GluI GCNGC 3 cut(s) 267, 561, 731
GsaI CCCAGC 1 cut(s) 140
GsuI CTGGAG 2 cut(s) 63, 137
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 1 cut(s) 200
HapII CCGG 1 cut(s) 81
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 601
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 749
HinfI GANTC 1 cut(s) 981
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 3 cut(s) 39, 57, 523
Hpy188I TCNGA 3 cut(s) 162, 478, 1014
Hpy188III TCNNGA 3 cut(s) 670, 833, 863
Hpy8I GTNNAC 3 cut(s) 39, 57, 523
HpyAV CCTTC 4 cut(s) 160, 166, 770, 860
HpyCH4III ACNGT 4 cut(s) 18, 637, 788, 988
HpyCH4IV ACGT 3 cut(s) 519, 601, 954
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 188, 197, 488, 752, 1049
HpyF3I CTNAG 3 cut(s) 159, 409, 747
HpySE526I ACGT 3 cut(s) 519, 601, 954
Hsp92I GRCGYC 1 cut(s) 601
HspAI GCGC 1 cut(s) 227
Kzo9I GATC 1 cut(s) 853
LguI GCTCTTC 1 cut(s) 236
LmnI GCTCC 3 cut(s) 727, 991, 1049
Lsp1109I GCAGC 3 cut(s) 278, 547, 717
LweI GCATC 2 cut(s) 469, 831
MaeI CTAG 3 cut(s) 224, 428, 606
MaeII ACGT 3 cut(s) 519, 601, 954
MaeIII GTNAC 5 cut(s) 62, 631, 719, 923, 982
MalI GATC 1 cut(s) 855
MboI GATC 1 cut(s) 853
MboII GAAGA 2 cut(s) 223, 653
MfeI CAATTG 3 cut(s) 110, 303, 876
MhlI GDGCHC 3 cut(s) 41, 257, 783
MlyI GAGTC 1 cut(s) 990
MmeI TCCRAC 3 cut(s) 326, 529, 817
MnlI CCTC 8 cut(s) 156, 161, 293, 334, 418, 875, 954, 1016
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 4 cut(s) 279, 596, 683, 1035
MslI CAYNNNNRTG 2 cut(s) 218, 239
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 2 cut(s) 155, 203
MunI CAATTG 3 cut(s) 110, 303, 876
Mva1269I GAATGC 2 cut(s) 225, 368
MvaI CCWGG 2 cut(s) 155, 203
MwoI GCNNNNNNNGC 6 cut(s) 77, 188, 197, 488, 752, 1049
NdeII GATC 1 cut(s) 853
NheI GCTAGC 1 cut(s) 223
NmuCI GTSAC 4 cut(s) 62, 631, 719, 982
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 2 cut(s) 669, 832
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 702
PctI GAATGC 2 cut(s) 225, 368
PkrI GCNGC 3 cut(s) 268, 562, 732
PleI GAGTC 1 cut(s) 989
PpsI GAGTC 1 cut(s) 989
Psp6I CCWGG 2 cut(s) 153, 201
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 2 cut(s) 153, 201
PspPI GGNCC 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 2 cut(s) 440, 1060
RsaNI GTAC 2 cut(s) 439, 1059
RseI CAYNNNNRTG 2 cut(s) 218, 239
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 4 cut(s) 279, 596, 683, 1035
SatI GCNGC 3 cut(s) 267, 561, 731
Sau3AI GATC 1 cut(s) 853
Sau96I GGNCC 1 cut(s) 199
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 1 cut(s) 990
ScrFI CCNGG 2 cut(s) 155, 203
SduI GDGCHC 3 cut(s) 41, 257, 783
SfaNI GCATC 2 cut(s) 469, 831
SmiMI CAYNNNNRTG 2 cut(s) 218, 239
SsiI CCGC 2 cut(s) 468, 740
SspMI CTAG 3 cut(s) 224, 428, 606
StyD4I CCNGG 2 cut(s) 153, 201
TaaI ACNGT 4 cut(s) 18, 637, 788, 988
TaiI ACGT 3 cut(s) 522, 604, 957
TatI WGTACW 1 cut(s) 1058
Tru1I TTAA 4 cut(s) 279, 596, 683, 1035
Tru9I TTAA 4 cut(s) 279, 596, 683, 1035
TscAI CASTG 2 cut(s) 46, 198
TseFI GTSAC 4 cut(s) 62, 631, 719, 982
TseI GCWGC 3 cut(s) 266, 560, 730
Tsp45I GTSAC 4 cut(s) 62, 631, 719, 982
TspDTI ATGAA 4 cut(s) 223, 348, 530, 658
TspGWI ACGGA 1 cut(s) 1062
TspRI CASTG 2 cut(s) 46, 198
VneI GTGCAC 1 cut(s) 37
XapI RAATTY 4 cut(s) 86, 130, 432, 973
XspI CTAG 3 cut(s) 224, 428, 606
ZraI GACGTC 1 cut(s) 602
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.