MD01G1206500.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000970
Physical Location & Seq
Reverse (-)
30185553 .. 30187919
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1206500.v1.1.491

Sequence Viewer

Length: 1470 bp
ATGGAGGAGCCGCTGCTTCACACAGCTGCTGCCGGCGCCGATGAGTTGAGCAATAGACCTCCTCTTTATGAAGGCGGGAACGAAGACTACGCACCGGTGAGGAGCTTTGATGCGTTCCAGCGTATGTTTTGGATAGAGACGGTAAAGCTGTGGCAAATAGGCGTTTCGTCAGTGATCACTATCATCTGTATGTATGGGACTAACGCAGTCATCCTGCTCTTTGCCGGTCATCTCGGAACTATCCAGCTCTCCGCCATCTCGATTTCTCTGGCTGTCATTTCCACATTCACTGATGGATTGATGCTTGGGATGGGGAGTGCCCTGGAGACATTGTGTGGACAGGCGTTTGGCGCTGGACAGGTTCATATGCTTGGGATCTACCTGCAACGATCATGGATAATTCTATTTGTAACCACCCTCTTCCTTTTGCCGGTTTACATCTTCGCCGTGCCGATTTTAAAGTGGCTAGGCCAAGAAGATGACATAGCGAACCTAGCCGGAAAATTCACACTCCAAATAATCCCTCAAATATTCTCACTTGCCATATATTTCCCAGCTCAGAAGTTCCTCCAAGCTCAGAGAAAAGTAAAGGTGCTGGCATGGATTGCATTTTTAGGTCTGGTTATACACATTGGAATGCTAGGGCTTTTCATGTATGAGTTTGATTGGGGCACATTGGGCGCAGCTGTGTCATTTAACATTACAAGATGGGGGATGGCAATTGCTCAAGTTGTGTATATTATGGGTTGGTGCAAAGAGGGTTGGACTGGATTTTCATGGCTCGCTTTCAAGGACATTTGGGAATTTGTAAGACTTTCTCTTGCCTCCGCAGTCATGCTCTGCCTTGAGATTTGGTACATGATGAGTATACTAATTCTCACCGGCAGTCTTTCTAATGCAGTTATAGCCATTGGTTCTCTTTCTATTTGTGTTCGTGTCTCTAATGAACTTGGGTCAGGACGTCCCAGAGCAACAAAATACTCTATCTATGTGACAGTATTTCAATCACTATTGATCGGAATTCTTTTCATGATTATTGTCTTAATGACTAGAGATGATTTCGCTATGCTTTTCACAAGTGACCAGGAGTTGCAACAAGCGGTTGCTAAGTTGGCATACCTTCTCGGTATAACTATGGTGCTCAACAGTGTCCAACTAGTAATATCAGGTGTTGCAATTGGAGGTGGATGGCAGACAATGGTTGCCTATCTAAACTTGGGTTGTTACTACATTTTTGGAATTCCTCTTGGATACCTTCTTTGCCATGTTGCAAAATGGGGAGTCATGGGACTATGGGGAGGCATGATATGTGGAACTGCTCTCCAAACCATACTTCTGCTAATTATATTTTACAAAACTAATTGGAACAAAGAGGTAATTAATCAATTCTTTATTTCAAATCTCTTCAACTTTGGTTTCTGCATTGCATTGTATATCCTGAAGTTCCTTATTGATTGGAATCGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

490

Amino Acids

54.5

Weight (kDa)

6.52

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 58 - 211 6.5e-29 MatE
MatE PF01554 309 - 419 1.9e-16 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 964
Acc36I ACCTGC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 35
AccI GTMKAC 1 cut(s) 868
AciI CCGC 5 cut(s) 11, 75, 252, 828, 1100
AclWI GGATC 1 cut(s) 383
AcsI RAATTY 4 cut(s) 503, 803, 1020, 1239
AcuI CTGAAG 1 cut(s) 1460
AcyI GRCGYC 2 cut(s) 36, 961
AfaI GTAC 1 cut(s) 857
AfiI CCNNNNNNNGG 1 cut(s) 430
AgeI ACCGGT 1 cut(s) 94
AgsI TTSAA 4 cut(s) 790, 1004, 1398, 1408
AhlI ACTAGT 1 cut(s) 1156
AjnI CCWGG 2 cut(s) 321, 1083
AjuI GAANNNNNNNTTGG 2 cut(s) 1317, 1349
AluBI AGCT 7 cut(s) 26, 105, 148, 247, 557, 575, 686
AluI AGCT 7 cut(s) 26, 105, 148, 247, 557, 575, 686
Alw21I GWGCWC 1 cut(s) 1143
Alw26I GTCTC 3 cut(s) 131, 320, 943
AlwI GGATC 1 cut(s) 383
AlwNI CAGNNNCTG 1 cut(s) 29
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 4 cut(s) 13, 26, 29, 683
ApoI RAATTY 4 cut(s) 503, 803, 1020, 1239
AseI ATTAAT 1 cut(s) 1380
AsiGI ACCGGT 1 cut(s) 94
AspLEI GCGC 3 cut(s) 38, 353, 683
AsuHPI GGTGA 2 cut(s) 109, 871
BaeGI GKGCMC 2 cut(s) 322, 674
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 1143
BbvI GCAGC 3 cut(s) 13, 16, 695
BccI CCATC 6 cut(s) 263, 287, 304, 702, 709, 1182
BceAI ACGGC 1 cut(s) 431
BciT130I CCWGG 2 cut(s) 323, 1085
BciVI GTATCC 1 cut(s) 1244
BclI TGATCA 1 cut(s) 174
BcoDI GTCTC 3 cut(s) 131, 320, 943
BcuI ACTAGT 1 cut(s) 1156
BfaI CTAG 5 cut(s) 467, 494, 641, 1050, 1157
BfoI RGCGCY 2 cut(s) 39, 354
BfuAI ACCTGC 1 cut(s) 390
BfuI GTATCC 1 cut(s) 1244
BisI GCNGC 5 cut(s) 11, 14, 27, 30, 684
BlsI GCNGC 5 cut(s) 12, 15, 28, 31, 685
Bme1390I CCNGG 2 cut(s) 323, 1085
BmiI GGNNCC 2 cut(s) 9, 37
BmrFI CCNGG 2 cut(s) 323, 1085
BmsI GCATC 2 cut(s) 100, 291
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 344
BpuEI CTTGAG 2 cut(s) 711, 866
BsaBI GATNNNNATC 2 cut(s) 179, 1458
BsaHI GRCGYC 2 cut(s) 36, 961
BsaJI CCNNGG 1 cut(s) 321
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse118I RCCGGY 5 cut(s) 32, 94, 224, 430, 881
Bse1I ACTGG 1 cut(s) 772
Bse3DI GCAATG 1 cut(s) 1422
Bse8I GATNNNNATC 2 cut(s) 179, 1458
BseBI CCWGG 2 cut(s) 323, 1085
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 4 cut(s) 210, 315, 720, 1193
BseJI GATNNNNATC 2 cut(s) 179, 1458
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMI GCAATG 1 cut(s) 1422
BseMII CTCAG 2 cut(s) 572, 590
BseNI ACTGG 1 cut(s) 772
BseRI GAGGAG 3 cut(s) 20, 51, 115
BseSI GKGCMC 2 cut(s) 322, 674
BseXI GCAGC 3 cut(s) 13, 16, 695
BseYI CCCAGC 1 cut(s) 553
BshFI GGCC 1 cut(s) 471
BshNI GGYRCC 1 cut(s) 35
BshTI ACCGGT 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 1143
BsiSI CCGG 6 cut(s) 33, 95, 225, 431, 498, 882
BslFI GGGAC 3 cut(s) 211, 948, 1302
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 3 cut(s) 131, 320, 943
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 3 cut(s) 211, 948, 1302
BsmI GAATGC 1 cut(s) 642
BsnI GGCC 1 cut(s) 471
Bsp1286I GDGCHC 3 cut(s) 322, 674, 1143
Bsp143I GATC 4 cut(s) 174, 375, 389, 1014
BspACI CCGC 5 cut(s) 11, 75, 252, 828, 1100
BspANI GGCC 1 cut(s) 471
BspCNI CTCAG 2 cut(s) 571, 589
BspHI TCATGA 1 cut(s) 1029
BspLI GGNNCC 2 cut(s) 9, 37
BspMI ACCTGC 1 cut(s) 390
BspPI GGATC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 1422
BsrFI RCCGGY 5 cut(s) 32, 94, 224, 430, 881
BsrI ACTGG 1 cut(s) 772
BssAI RCCGGY 5 cut(s) 32, 94, 224, 430, 881
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 4 cut(s) 174, 375, 389, 1014
BssNAI GTATAC 1 cut(s) 869
BssNI GRCGYC 2 cut(s) 36, 961
Bst1107I GTATAC 1 cut(s) 869
Bst2UI CCWGG 2 cut(s) 323, 1085
Bst4CI ACNGT 3 cut(s) 142, 997, 1148
Bst6I CTCTTC 2 cut(s) 425, 1409
BstACI GRCGYC 2 cut(s) 36, 961
BstAPI GCANNNNNTGC 1 cut(s) 605
BstC8I GCNNGC 3 cut(s) 34, 597, 783
BstDEI CTNAG 3 cut(s) 558, 576, 1107
BstF5I GGATG 4 cut(s) 210, 315, 720, 1193
BstH2I RGCGCY 2 cut(s) 39, 354
BstHHI GCGC 3 cut(s) 38, 353, 683
BstKTI GATC 4 cut(s) 177, 378, 392, 1017
BstMAI GTCTC 3 cut(s) 131, 320, 943
BstMBI GATC 4 cut(s) 174, 375, 389, 1014
BstMWI GCNNNNNNNGC 7 cut(s) 35, 350, 494, 605, 678, 905, 1112
BstNI CCWGG 2 cut(s) 323, 1085
BstSCI CCNGG 2 cut(s) 321, 1083
BstSLI GKGCMC 2 cut(s) 322, 674
BstV1I GCAGC 3 cut(s) 13, 16, 695
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 375
BstYI RGATCY 1 cut(s) 375
BstZ17I GTATAC 1 cut(s) 869
BsuI GTATCC 1 cut(s) 1244
BsuRI GGCC 1 cut(s) 471
BtsCI GGATG 4 cut(s) 210, 315, 720, 1193
BtsIMutI CAGTG 3 cut(s) 177, 288, 1153
BveI ACCTGC 1 cut(s) 390
Cac8I GCNNGC 3 cut(s) 34, 597, 783
CaiI CAGNNNCTG 1 cut(s) 29
CciI TCATGA 1 cut(s) 1029
CfoI GCGC 3 cut(s) 38, 353, 683
Cfr10I RCCGGY 5 cut(s) 32, 94, 224, 430, 881
Csp6I GTAC 1 cut(s) 856
CspAI ACCGGT 1 cut(s) 94
CviQI GTAC 1 cut(s) 856
DdeI CTNAG 3 cut(s) 558, 576, 1107
DinI GGCGCC 1 cut(s) 37
DpnI GATC 4 cut(s) 176, 377, 391, 1016
DpnII GATC 4 cut(s) 174, 375, 389, 1014
DraI TTTAAA 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 425, 1409
EarI CTCTTC 2 cut(s) 425, 1409
EciI GGCGGA 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 1460
EcoRI GAATTC 2 cut(s) 1020, 1239
EcoRII CCWGG 2 cut(s) 321, 1083
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
Esp3I CGTCTC 1 cut(s) 131
FaqI GGGAC 3 cut(s) 211, 948, 1302
FauI CCCGC 1 cut(s) 68
FauNDI CATATG 1 cut(s) 366
FbaI TGATCA 1 cut(s) 174
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 5 cut(s) 11, 14, 27, 30, 684
FokI GGATG 4 cut(s) 197, 322, 727, 1200
Fsp4HI GCNGC 5 cut(s) 11, 14, 27, 30, 684
FspBI CTAG 5 cut(s) 467, 494, 641, 1050, 1157
GlaI GCGC 3 cut(s) 37, 352, 682
GluI GCNGC 5 cut(s) 11, 14, 27, 30, 684
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 1 cut(s) 344
HaeII RGCGCY 2 cut(s) 39, 354
HaeIII GGCC 1 cut(s) 471
HapII CCGG 6 cut(s) 33, 95, 225, 431, 498, 882
HhaI GCGC 3 cut(s) 38, 353, 683
Hin1I GRCGYC 2 cut(s) 36, 961
Hin6I GCGC 3 cut(s) 36, 351, 681
HinP1I GCGC 3 cut(s) 36, 351, 681
HinfI GANTC 2 cut(s) 1281, 1459
HpaII CCGG 6 cut(s) 33, 95, 225, 431, 498, 882
HphI GGTGA 2 cut(s) 109, 871
Hpy166II GTNNAC 3 cut(s) 338, 436, 869
Hpy188I TCNGA 4 cut(s) 236, 561, 579, 1019
Hpy188III TCNNGA 4 cut(s) 259, 957, 1030, 1438
Hpy8I GTNNAC 3 cut(s) 338, 436, 869
HpyAV CCTTC 3 cut(s) 65, 1130, 1265
HpyCH4III ACNGT 3 cut(s) 142, 997, 1148
HpyCH4IV ACGT 1 cut(s) 961
HpyCH4V TGCA 9 cut(s) 385, 608, 753, 899, 1093, 1175, 1271, 1422, 1427
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 350, 494, 605, 678, 905, 1112
HpyF3I CTNAG 3 cut(s) 558, 576, 1107
HpySE526I ACGT 1 cut(s) 961
Hsp92I GRCGYC 2 cut(s) 36, 961
HspAI GCGC 3 cut(s) 36, 351, 681
KasI GGCGCC 1 cut(s) 35
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 4 cut(s) 174, 375, 389, 1014
LmnI GCTCC 2 cut(s) 7, 102
Lsp1109I GCAGC 3 cut(s) 13, 16, 695
LweI GCATC 2 cut(s) 100, 291
MaeI CTAG 5 cut(s) 467, 494, 641, 1050, 1157
MaeII ACGT 1 cut(s) 961
MaeIII GTNAC 4 cut(s) 409, 991, 1079, 1223
MalI GATC 4 cut(s) 176, 377, 391, 1016
MboI GATC 4 cut(s) 174, 375, 389, 1014
MboII GAAGA 5 cut(s) 95, 412, 433, 488, 1396
MfeI CAATTG 2 cut(s) 720, 1176
MflI RGATCY 1 cut(s) 375
MhlI GDGCHC 3 cut(s) 322, 674, 1143
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 1 cut(s) 1290
MmeI TCCRAC 2 cut(s) 743, 1177
MroNI GCCGGC 1 cut(s) 32
MseI TTAA 5 cut(s) 458, 696, 1043, 1380, 1468
MslI CAYNNNNRTG 2 cut(s) 188, 635
MspA1I CMGCKG 3 cut(s) 13, 26, 686
MspI CCGG 6 cut(s) 33, 95, 225, 431, 498, 882
MspR9I CCNGG 2 cut(s) 323, 1085
MunI CAATTG 2 cut(s) 720, 1176
Mva1269I GAATGC 1 cut(s) 642
MvaI CCWGG 2 cut(s) 323, 1085
MwoI GCNNNNNNNGC 7 cut(s) 35, 350, 494, 605, 678, 905, 1112
NaeI GCCGGC 1 cut(s) 34
NarI GGCGCC 1 cut(s) 36
NdeI CATATG 1 cut(s) 366
NdeII GATC 4 cut(s) 174, 375, 389, 1014
NgoMIV GCCGGC 1 cut(s) 32
NlaIV GGNNCC 2 cut(s) 9, 37
NmuCI GTSAC 2 cut(s) 991, 1079
PagI TCATGA 1 cut(s) 1029
PctI GAATGC 1 cut(s) 642
PdiI GCCGGC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 1459
PinAI ACCGGT 1 cut(s) 94
PkrI GCNGC 5 cut(s) 12, 15, 28, 31, 685
PleI GAGTC 1 cut(s) 1289
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 1289
PshBI ATTAAT 1 cut(s) 1380
Psp6I CCWGG 2 cut(s) 321, 1083
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 2 cut(s) 321, 1083
PspN4I GGNNCC 2 cut(s) 9, 37
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 375
PvuII CAGCTG 2 cut(s) 26, 686
RsaI GTAC 1 cut(s) 857
RsaNI GTAC 1 cut(s) 856
RseI CAYNNNNRTG 2 cut(s) 188, 635
SaqAI TTAA 5 cut(s) 458, 696, 1043, 1380, 1468
SatI GCNGC 5 cut(s) 11, 14, 27, 30, 684
Sau3AI GATC 4 cut(s) 174, 375, 389, 1014
SchI GAGTC 1 cut(s) 1290
ScrFI CCNGG 2 cut(s) 323, 1085
SduI GDGCHC 3 cut(s) 322, 674, 1143
SfaNI GCATC 2 cut(s) 100, 291
SfoI GGCGCC 1 cut(s) 37
SgrAI CRCCGGYG 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 188, 635
SmlI CTYRAG 2 cut(s) 726, 845
SmoI CTYRAG 2 cut(s) 726, 845
SpeI ACTAGT 1 cut(s) 1156
SsiI CCGC 5 cut(s) 11, 75, 252, 828, 1100
SspDI GGCGCC 1 cut(s) 35
SspI AATATT 1 cut(s) 531
SspMI CTAG 5 cut(s) 467, 494, 641, 1050, 1157
StyD4I CCNGG 2 cut(s) 321, 1083
TaaI ACNGT 3 cut(s) 142, 997, 1148
TaiI ACGT 1 cut(s) 964
TaqI TCGA 1 cut(s) 260
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 1 cut(s) 1459
Tru1I TTAA 5 cut(s) 458, 696, 1043, 1380, 1468
Tru9I TTAA 5 cut(s) 458, 696, 1043, 1380, 1468
TscAI CASTG 3 cut(s) 177, 295, 1153
TseFI GTSAC 2 cut(s) 991, 1079
TseI GCWGC 4 cut(s) 13, 26, 29, 683
Tsp45I GTSAC 2 cut(s) 991, 1079
TspDTI ATGAA 6 cut(s) 84, 353, 640, 765, 960, 1018
TspRI CASTG 3 cut(s) 177, 295, 1153
VspI ATTAAT 1 cut(s) 1380
XapI RAATTY 4 cut(s) 503, 803, 1020, 1239
XmiI GTMKAC 1 cut(s) 868
XspI CTAG 5 cut(s) 467, 494, 641, 1050, 1157
ZraI GACGTC 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.