RLG00000026475

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000970
Physical Location & Seq
Forward (+)
3247287 .. 3249951
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026475

Sequence Viewer

Length: 1200 bp
ATGGGGAGTGCTTTGGAGACCTTGGCTGGACAGGCGTTTGGCGCAGGACAGATTCATATGCTTGGGATATACATGCAGCGCTCATGTATAGTCCTTCTTGTAACTTGCTTCCTCCTGTTGCCATTTTACATCTTTGCCACTCCCATTTTAAAGTTATTAGGCCAAGAAGATGACATAGCCGATCTCGCTGGAGAGTTCACACTGCAAATAATTCCTCAGTTGTTCTCACTTGCCATTAACTTTCCTGCTCAGAAGTTCCTTCAGGCTCAGAGAAAGCTCAAAGTCATGACGTGGATTTCGGTGGTGGCGTTGATCGTACACGTTGGGCTACTTTTCTTCTTCATTTTTGGGTTAGATTGGGGTACCTTAGGTGCAGCTGTGGCATATAATATTACCAGATGGGGCATTGCAATTGCTCAAGTTGTATATATTATGGTCTGGTGCAATGAGGGTTGGACTGGGTTCTCATGGTTGGCATTCAAGGATATGTGGTCCTTTGTTAGGCTTTCAATTGCCTCTGCTGTCATGCTTTGCCTTGAGATATGGTACTTCATGAGTATGATGCTTCTTACTGGCAACCTAAACGATGCAGTTCTAGCAGTTAGTTCCCTTTCTATTTGCCTCAACATTAACGGGTGGGAAATAATGCTGTTTGTTGGAATAAATATTGCTATAAGTGTTCGAGTATCGAATGAGCTTGGCTTAGGACGCCCAAGAGCGGCCAAATATGCAGTCTATGTGACAGTATTTCAGTCTTTCCTAATGGGGATTTTTTTCATGATTCTTATCTTTTTATTCAAAGATAGCTTCTCTGCACTTTTTACTACTGACAAGGAGCTGCAACAAGCGGTTGCACACCTAGCATACCTTCTCGGCGCAACTATGTTGCTCAACAGTGTCCAGCCAGTAATATCAGGTGTTGCGGTTGGCGGTGGATGGCAGGCAACCGTGGCATATATAAACTTGGGTTGTTACTACATTTTTGGAATTCCTCTCGGATACTTGCTTAGTCACGTCGCAGATTTCGGAGTGATGGGACTTTGGGGGGGTATGATATGTGGAACTGCTCTTCAGACCTTGCTTCTTTTATTTGTGCTTTACAAAACCAACTGGAACAAAGAGGTGGAGCAAGCATCAACACGCGTGCAGAAGTGGGGATTAGCTAACAAAACAGAGACTACAGCAGAAAGTGCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

400

Amino Acids

44.11

Weight (kDa)

6.42

Isoelectric Point (pI)

28.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 110 2.8e-20 MatE
MatE PF01554 173 - 335 6.5e-28 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 719
AccII CGCG 1 cut(s) 1143
AciI CCGC 4 cut(s) 719, 848, 923, 930
AcoI YGGCCR 1 cut(s) 720
AcsI RAATTY 1 cut(s) 987
AcuI CTGAAG 2 cut(s) 245, 1055
AcyI GRCGYC 1 cut(s) 709
AfaI GTAC 3 cut(s) 318, 364, 548
AfeI AGCGCT 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 501, 718
AflIII ACRYGT 2 cut(s) 319, 1141
AgsI TTSAA 3 cut(s) 481, 510, 799
AjiI CACGTC 2 cut(s) 291, 1015
AluBI AGCT 6 cut(s) 277, 377, 697, 807, 838, 1163
AluI AGCT 6 cut(s) 277, 377, 697, 807, 838, 1163
Alw26I GTCTC 2 cut(s) 11, 1169
Aor51HI AGCGCT 1 cut(s) 80
AoxI GGCC 2 cut(s) 160, 720
ApeKI GCWGC 3 cut(s) 76, 374, 838
ApoI RAATTY 1 cut(s) 987
ArsI GACNNNNNNTTYG 4 cut(s) 280, 312, 717, 749
Asp718I GGTACC 1 cut(s) 362
AspLEI GCGC 3 cut(s) 44, 81, 878
AspS9I GGNCC 1 cut(s) 492
AvaII GGWCC 1 cut(s) 492
AxyI CCTNAGG 1 cut(s) 367
BanI GGYRCC 1 cut(s) 362
BbvI GCAGC 3 cut(s) 88, 386, 825
BccI CCATC 3 cut(s) 393, 930, 1027
BciVI GTATCC 1 cut(s) 992
BcoDI GTCTC 2 cut(s) 11, 1169
BfaI CTAG 2 cut(s) 596, 860
BfmI CTRYAG 1 cut(s) 1179
BfoI RGCGCY 1 cut(s) 82
BfuI GTATCC 1 cut(s) 992
BisI GCNGC 4 cut(s) 77, 375, 720, 839
BlsI GCNGC 4 cut(s) 78, 376, 721, 840
Bme18I GGWCC 1 cut(s) 492
BmgBI CACGTC 2 cut(s) 291, 1015
BmgT120I GGNCC 1 cut(s) 492
BmiI GGNNCC 1 cut(s) 364
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 3 cut(s) 552, 577, 1142
BmuI ACTGGG 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 703
BpuEI CTTGAG 2 cut(s) 402, 557
BsaBI GATNNNNATC 1 cut(s) 785
BsaHI GRCGYC 1 cut(s) 709
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 21, 948
Bsc4I CCNNNNNNNGG 2 cut(s) 501, 718
Bse1I ACTGG 4 cut(s) 463, 577, 905, 1115
Bse21I CCTNAGG 1 cut(s) 367
Bse3DI GCAATG 2 cut(s) 405, 451
Bse8I GATNNNNATC 1 cut(s) 785
BseDI CCNNGG 2 cut(s) 21, 948
BseGI GGATG 1 cut(s) 941
BseJI GATNNNNATC 1 cut(s) 785
BseLI CCNNNNNNNGG 2 cut(s) 501, 718
BseMI GCAATG 2 cut(s) 405, 451
BseMII CTCAG 3 cut(s) 230, 263, 281
BseNI ACTGG 4 cut(s) 463, 577, 905, 1115
BseXI GCAGC 3 cut(s) 88, 386, 825
BsgI GTGCAG 3 cut(s) 393, 798, 1166
Bsh1236I CGCG 1 cut(s) 1143
BshFI GGCC 2 cut(s) 162, 722
BshNI GGYRCC 1 cut(s) 362
BslFI GGGAC 1 cut(s) 1050
BslI CCNNNNNNNGG 2 cut(s) 501, 718
BsmAI GTCTC 2 cut(s) 11, 1169
BsmFI GGGAC 1 cut(s) 1050
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 2 cut(s) 162, 722
Bso31I GGTCTC 1 cut(s) 11
Bsp143I GATC 2 cut(s) 181, 312
BspACI CCGC 4 cut(s) 719, 848, 923, 930
BspANI GGCC 2 cut(s) 162, 722
BspCNI CTCAG 3 cut(s) 229, 262, 280
BspFNI CGCG 1 cut(s) 1143
BspHI TCATGA 3 cut(s) 285, 552, 777
BspLI GGNNCC 1 cut(s) 364
BspQI GCTCTTC 1 cut(s) 1074
BspT107I GGYRCC 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 719
BsrDI GCAATG 2 cut(s) 405, 451
BsrI ACTGG 4 cut(s) 463, 577, 905, 1115
BssECI CCNNGG 2 cut(s) 21, 948
BssMI GATC 2 cut(s) 181, 312
BssNI GRCGYC 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 21
Bst4CI ACNGT 3 cut(s) 745, 896, 949
Bst6I CTCTTC 1 cut(s) 1074
BstACI GRCGYC 1 cut(s) 709
BstAPI GCANNNNNTGC 1 cut(s) 1190
BstC8I GCNNGC 3 cut(s) 942, 1131, 1145
BstDEI CTNAG 6 cut(s) 216, 249, 267, 367, 703, 1007
BstDSI CCRYGG 1 cut(s) 948
BstENI CCTNNNNNAGG 1 cut(s) 499
BstF5I GGATG 1 cut(s) 941
BstFNI CGCG 1 cut(s) 1143
BstH2I RGCGCY 1 cut(s) 82
BstHHI GCGC 3 cut(s) 44, 81, 878
BstKTI GATC 2 cut(s) 184, 315
BstMAI GTCTC 2 cut(s) 11, 1169
BstMBI GATC 2 cut(s) 181, 312
BstNSI RCATGY 1 cut(s) 76
BstSFI CTRYAG 1 cut(s) 1179
BstUI CGCG 1 cut(s) 1143
BstV1I GCAGC 3 cut(s) 88, 386, 825
Bsu36I CCTNAGG 1 cut(s) 367
BsuI GTATCC 1 cut(s) 992
BsuRI GGCC 2 cut(s) 162, 722
BtgI CCRYGG 1 cut(s) 948
BtrI CACGTC 2 cut(s) 291, 1015
BtsCI GGATG 1 cut(s) 941
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 2 cut(s) 200, 901
Cac8I GCNNGC 3 cut(s) 942, 1131, 1145
CciI TCATGA 3 cut(s) 285, 552, 777
CfoI GCGC 3 cut(s) 44, 81, 878
Cfr13I GGNCC 1 cut(s) 492
CseI GACGC 1 cut(s) 717
Csp6I GTAC 3 cut(s) 317, 363, 547
CviAII CATG 7 cut(s) 73, 84, 286, 468, 526, 553, 778
CviQI GTAC 3 cut(s) 317, 363, 547
DdeI CTNAG 6 cut(s) 216, 249, 267, 367, 703, 1007
DpnI GATC 2 cut(s) 183, 314
DpnII GATC 2 cut(s) 181, 312
DraI TTTAAA 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 720
Eam1104I CTCTTC 1 cut(s) 1074
EarI CTCTTC 1 cut(s) 1074
Eco130I CCWWGG 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 492
Eco47III AGCGCT 1 cut(s) 80
Eco57I CTGAAG 2 cut(s) 245, 1055
Eco81I CCTNAGG 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 499
EcoRI GAATTC 1 cut(s) 987
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 7 cut(s) 76, 87, 289, 471, 529, 556, 781
FaqI GGGAC 1 cut(s) 1050
FatI CATG 7 cut(s) 72, 83, 285, 467, 525, 552, 777
FauNDI CATATG 1 cut(s) 57
Fnu4HI GCNGC 4 cut(s) 77, 375, 720, 839
FokI GGATG 1 cut(s) 948
Fsp4HI GCNGC 4 cut(s) 77, 375, 720, 839
FspBI CTAG 2 cut(s) 596, 860
GlaI GCGC 3 cut(s) 43, 80, 877
GluI GCNGC 4 cut(s) 77, 375, 720, 839
GsuI CTGGAG 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 82
HaeIII GGCC 2 cut(s) 162, 722
HgaI GACGC 1 cut(s) 717
HhaI GCGC 3 cut(s) 44, 81, 878
Hin1I GRCGYC 1 cut(s) 709
Hin1II CATG 7 cut(s) 76, 87, 289, 471, 529, 556, 781
Hin6I GCGC 3 cut(s) 42, 79, 876
HinP1I GCGC 3 cut(s) 42, 79, 876
HinfI GANTC 2 cut(s) 52, 781
Hpy166II GTNNAC 2 cut(s) 198, 319
Hpy188I TCNGA 5 cut(s) 252, 270, 998, 1028, 1074
Hpy188III TCNNGA 3 cut(s) 286, 553, 778
Hpy8I GTNNAC 2 cut(s) 198, 319
Hpy99I CGWCG 1 cut(s) 1019
HpyAV CCTTC 3 cut(s) 104, 269, 878
HpyCH4III ACNGT 3 cut(s) 745, 896, 949
HpyCH4IV ACGT 3 cut(s) 290, 321, 1014
HpyF3I CTNAG 6 cut(s) 216, 249, 267, 367, 703, 1007
HpySE526I ACGT 3 cut(s) 290, 321, 1014
Hsp92I GRCGYC 1 cut(s) 709
Hsp92II CATG 7 cut(s) 76, 87, 289, 471, 529, 556, 781
HspAI GCGC 3 cut(s) 42, 79, 876
KpnI GGTACC 1 cut(s) 366
Kzo9I GATC 2 cut(s) 181, 312
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 2 cut(s) 835, 1126
Lsp1109I GCAGC 3 cut(s) 88, 386, 825
LweI GCATC 3 cut(s) 552, 577, 1142
MaeI CTAG 2 cut(s) 596, 860
MaeII ACGT 3 cut(s) 290, 321, 1014
MaeIII GTNAC 4 cut(s) 100, 739, 971, 1010
MalI GATC 2 cut(s) 183, 314
MbiI CCGCTC 1 cut(s) 719
MboI GATC 2 cut(s) 181, 312
MboII GAAGA 4 cut(s) 179, 328, 331, 1061
MfeI CAATTG 2 cut(s) 411, 510
MluCI AATT 4 cut(s) 210, 411, 510, 987
MluI ACGCGT 1 cut(s) 1141
MmeI TCCRAC 2 cut(s) 434, 637
MnlI CCTC 7 cut(s) 122, 225, 442, 526, 632, 1002, 1114
MseI TTAA 3 cut(s) 149, 237, 630
MslI CAYNNNNRTG 1 cut(s) 557
MspA1I CMGCKG 1 cut(s) 377
MunI CAATTG 2 cut(s) 411, 510
Mva1269I GAATGC 1 cut(s) 476
MvnI CGCG 1 cut(s) 1143
NdeI CATATG 1 cut(s) 57
NdeII GATC 2 cut(s) 181, 312
NlaIII CATG 7 cut(s) 76, 87, 289, 471, 529, 556, 781
NlaIV GGNNCC 1 cut(s) 364
NmeAIII GCCGAG 1 cut(s) 852
NmuCI GTSAC 2 cut(s) 739, 1010
NspI RCATGY 1 cut(s) 76
PagI TCATGA 3 cut(s) 285, 552, 777
PciSI GCTCTTC 1 cut(s) 1074
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PctI GAATGC 1 cut(s) 476
PfeI GAWTC 2 cut(s) 52, 781
PkrI GCNGC 4 cut(s) 78, 376, 721, 840
PspN4I GGNNCC 1 cut(s) 364
PspPI GGNCC 1 cut(s) 492
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 3 cut(s) 318, 364, 548
RsaNI GTAC 3 cut(s) 317, 363, 547
RseI CAYNNNNRTG 1 cut(s) 557
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 3 cut(s) 149, 237, 630
SatI GCNGC 4 cut(s) 77, 375, 720, 839
Sau3AI GATC 2 cut(s) 181, 312
Sau96I GGNCC 1 cut(s) 492
SfaNI GCATC 3 cut(s) 552, 577, 1142
SfcI CTRYAG 1 cut(s) 1179
SinI GGWCC 1 cut(s) 492
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 2 cut(s) 417, 536
SmoI CTYRAG 2 cut(s) 417, 536
Sse9I AATT 4 cut(s) 210, 411, 510, 987
SsiI CCGC 4 cut(s) 719, 848, 923, 930
SspI AATATT 2 cut(s) 391, 667
SspMI CTAG 2 cut(s) 596, 860
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 3 cut(s) 745, 896, 949
TaiI ACGT 3 cut(s) 293, 324, 1017
TaqI TCGA 2 cut(s) 682, 689
TasI AATT 4 cut(s) 210, 411, 510, 987
TauI GCSGC 1 cut(s) 722
TfiI GAWTC 2 cut(s) 52, 781
Tru1I TTAA 3 cut(s) 149, 237, 630
Tru9I TTAA 3 cut(s) 149, 237, 630
TscAI CASTG 2 cut(s) 207, 901
TseFI GTSAC 2 cut(s) 739, 1010
TseI GCWGC 3 cut(s) 76, 374, 838
Tsp45I GTSAC 2 cut(s) 739, 1010
TspDTI ATGAA 4 cut(s) 44, 331, 541, 766
TspRI CASTG 2 cut(s) 207, 901
VpaK11BI GGWCC 1 cut(s) 492
XagI CCTNNNNNAGG 1 cut(s) 499
XapI RAATTY 1 cut(s) 987
XceI RCATGY 1 cut(s) 76
XspI CTAG 2 cut(s) 596, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.