pycom07g25180

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000970
Physical Location & Seq
Reverse (-)
25601561 .. 25605152
3592 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25180.7

Sequence Viewer

Length: 1473 bp
ATGGAGACGCCACTGCTTCACGCAGTGAACGGCGGCGAGTTCCATGACTACGAGCCGGTGAGGAACTTCAGGGATGCTCGGTCGGTGGCGTGGAATGAAACCAAGAAGCTGTGGAAGATTGCAGGGCCCATCGCCTTCACTATCATCTGCAACTTTGGCAACAACACAGCTACCACCATGTTTGTCGGCCATCTCGGAAACCTCCAGCTCTCCGCCGTTTCCATCTCTCTCTCCGTCATCTGCACCTTCTCATTCGGCTTCATGCTTGGCATGGGGAGTGCGTTAGAGACGCTTTGTGGCCAAGCTTTCGGGGCCGGACATGTTAACATGCTCGGCGTTTACATGCAAAGATCATGGATCATTCTGTTCACCAGCTGTGTTATCCTCACGCCAATCTATATCTTCTCCGCCCCGATTTTGAAGCTTCTCGGGCAGGATGATGACGTGGCTAATCTTGCTGGAACATTCACTATATATTGCATACCGCAGTTGTTCTCCCTTGCCTTCAACTTCCCTGCCCAGAAGTTCCTTCAGGCTCAAAGCAAAGTTCTCGTGCTTGCGTGGATTGGATTTATCTCGCTTCTTGTACAAATTGGATTGCTTTCTCTCTTCATTTACGTGTTCGATTGGGGTATATATGGTGCAGGCATTGCATTTGGCATCACAGGTTGGGTAATTGTGATTGCTCAAAATATTTACATAATGAAATGGTGCAAGGAAGGGTGGACAGGGTTTTCGTGGTTGGCGTTCAAAGACATATGGGCGTTTGTCCGGCTTTCACTTGAGTCCGCCTTGATGCTTTGCCTGGAGATTTGGTATATGATGACTCTTATCCTTGCTACAGGCCACCTTAGTAATGCCGTACTTTCTATTGACTCGCTCGCTATTTGCTTAAATTTCAACGGTTTGGAAGCAATGTTGTTCATTGGAATCAATGCTGCTATAAGTGTTCGGGTCTCCAACGAACTTGGATTGGCACGTCCAAGAGCAGCTAAATATTCAGTCTACGTGACGGTTGGTCAGTCTCTGCTCATCGGAATCGTTTTCATGGTTGTGATCTTGATAACCAAAGACTACTTTTCTGTCCTGTACACAAACGACGAGGAATTGCAACATTCTGTTTCCAATCTAGCAATTCTTCTCGGAGTGACTATGCTTCTCAATAGCGTCCAACCAGTTATTTCCGGTGTTGCCATCGGAGGCGGATGGCAGGGACTGGTGGCTTATGTAAACTTGGGTTGTTACTACATTATCGGGCTCCCGCTCGGTATCCTTCTTGGCTATGTAGCAAAATGGGGAGTAAAGGGAATTTGGGGAGGCATGATATGCGGGACTGCTCTTCAAACCTTGCTGCTTATGATTGTACTTTACAGAACCAACTGGAACAAGGAGGTTGAACAAACAACGGACCGGATGAAGAAGTGGACAGGGCAAGACTACGGTGACGAGGAGATAGCTGCTGTTAGCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

491

Amino Acids

53.92

Weight (kDa)

6.08

Isoelectric Point (pI)

23.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1264
AccI GTMKAC 1 cut(s) 1005
AciI CCGC 8 cut(s) 33, 213, 408, 485, 789, 1203, 1262, 1329
AclWI GGATC 1 cut(s) 365
AcoI YGGCCR 2 cut(s) 187, 298
AcsI RAATTY 2 cut(s) 895, 1308
AcuI CTGAAG 2 cut(s) 52, 515
AcyI GRCGYC 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 25
AfaI GTAC 4 cut(s) 588, 864, 1091, 1365
AflIII ACRYGT 2 cut(s) 319, 618
AgsI TTSAA 6 cut(s) 421, 508, 751, 901, 1343, 1397
AhdI GACNNNNNGTC 1 cut(s) 1017
AjiI CACGTC 2 cut(s) 445, 980
AjnI CCWGG 1 cut(s) 804
AluBI AGCT 8 cut(s) 109, 170, 208, 305, 375, 424, 992, 1457
AluI AGCT 8 cut(s) 109, 170, 208, 305, 375, 424, 992, 1457
Alw26I GTCTC 3 cut(s) 281, 961, 1029
AlwI GGATC 1 cut(s) 365
AlwNI CAGNNNCTG 2 cut(s) 1027, 1216
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 5 cut(s) 125, 187, 298, 312, 844
ApaI GGGCCC 1 cut(s) 129
ApeKI GCWGC 4 cut(s) 938, 989, 1351, 1457
ApoI RAATTY 2 cut(s) 895, 1308
ArsI GACNNNNNNTTYG 2 cut(s) 718, 750
AspS9I GGNCC 4 cut(s) 125, 126, 312, 1408
AsuHPI GGTGA 3 cut(s) 70, 361, 1454
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 1 cut(s) 1408
BaeGI GKGCMC 1 cut(s) 129
BalI TGGCCA 1 cut(s) 300
BanII GRGCYC 2 cut(s) 129, 1260
BauI CACGAG 1 cut(s) 551
BbvI GCAGC 4 cut(s) 925, 1001, 1338, 1444
BccI CCATC 5 cut(s) 137, 198, 230, 1200, 1202
BceAI ACGGC 3 cut(s) 46, 200, 845
BcgI CGANNNNNNTGC 2 cut(s) 532, 566
BciT130I CCWGG 1 cut(s) 806
BciVI GTATCC 1 cut(s) 1280
BcoDI GTCTC 3 cut(s) 281, 961, 1029
BfaI CTAG 1 cut(s) 1130
BfmI CTRYAG 1 cut(s) 840
BfuI GTATCC 1 cut(s) 1280
BisI GCNGC 5 cut(s) 34, 939, 990, 1352, 1458
BlsI GCNGC 5 cut(s) 35, 940, 991, 1353, 1459
Bme1390I CCNGG 1 cut(s) 806
Bme18I GGWCC 1 cut(s) 1408
BmeRI GACNNNNNGTC 1 cut(s) 1017
BmeT110I CYCGRG 1 cut(s) 428
BmgBI CACGTC 2 cut(s) 445, 980
BmgT120I GGNCC 4 cut(s) 125, 126, 312, 1408
BmiI GGNNCC 3 cut(s) 127, 313, 1259
BmrFI CCNGG 1 cut(s) 806
BmsI GCATC 3 cut(s) 64, 669, 786
BpmI CTGGAG 2 cut(s) 188, 827
BpuEI CTTGAG 1 cut(s) 803
BsaAI YACGTR 2 cut(s) 619, 1009
BsaHI GRCGYC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 961
BsaWI WCCGGW 2 cut(s) 1184, 1410
BsaXI ACNNNNNCTCC 2 cut(s) 800, 830
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 1175, 1221, 1385
Bse3DI GCAATG 2 cut(s) 648, 921
BseBI CCWGG 1 cut(s) 806
BseGI GGATG 4 cut(s) 79, 442, 1211, 1419
BseMI GCAATG 2 cut(s) 648, 921
BseNI ACTGG 3 cut(s) 1175, 1221, 1385
BseRI GAGGAG 1 cut(s) 1463
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 4 cut(s) 925, 1001, 1338, 1444
BsgI GTGCAG 2 cut(s) 226, 663
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 5 cut(s) 127, 189, 300, 314, 846
BsiEI CGRYCG 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 5 cut(s) 56, 315, 772, 1185, 1411
BslFI GGGAC 2 cut(s) 1227, 1345
BsmAI GTCTC 3 cut(s) 281, 961, 1029
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 2 cut(s) 1227, 1345
BsnI GGCC 5 cut(s) 127, 189, 300, 314, 846
Bso31I GGTCTC 1 cut(s) 961
BsoBI CYCGRG 1 cut(s) 428
Bsp120I GGGCCC 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 129, 1260
Bsp1407I TGTACA 2 cut(s) 586, 1089
Bsp143I GATC 3 cut(s) 350, 357, 1056
BspACI CCGC 8 cut(s) 33, 213, 408, 485, 789, 1203, 1262, 1329
BspANI GGCC 5 cut(s) 127, 189, 300, 314, 846
BspLI GGNNCC 3 cut(s) 127, 313, 1259
BspPI GGATC 1 cut(s) 365
BspQI GCTCTTC 1 cut(s) 1344
BspTNI GGTCTC 1 cut(s) 961
BsrBI CCGCTC 1 cut(s) 1264
BsrDI GCAATG 2 cut(s) 648, 921
BsrFI RCCGGY 1 cut(s) 55
BsrGI TGTACA 2 cut(s) 586, 1089
BsrI ACTGG 3 cut(s) 1175, 1221, 1385
BssAI RCCGGY 1 cut(s) 55
BssMI GATC 3 cut(s) 350, 357, 1056
BssNI GRCGYC 1 cut(s) 8
BssSI CACGAG 1 cut(s) 551
Bst2BI CACGAG 1 cut(s) 551
Bst2UI CCWGG 1 cut(s) 806
Bst4CI ACNGT 3 cut(s) 905, 1015, 1442
Bst6I CTCTTC 2 cut(s) 614, 1344
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 2 cut(s) 650, 1326
BstAUI TGTACA 2 cut(s) 586, 1089
BstBAI YACGTR 2 cut(s) 619, 1009
BstC8I GCNNGC 3 cut(s) 558, 646, 882
BstDEI CTNAG 1 cut(s) 851
BstF5I GGATG 4 cut(s) 79, 442, 1211, 1419
BstKTI GATC 3 cut(s) 353, 360, 1059
BstMAI GTCTC 3 cut(s) 281, 961, 1029
BstMBI GATC 3 cut(s) 350, 357, 1056
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 6 cut(s) 156, 311, 430, 455, 650, 1326
BstNI CCWGG 1 cut(s) 806
BstNSI RCATGY 3 cut(s) 323, 331, 346
BstSCI CCNGG 1 cut(s) 804
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 1 cut(s) 129
BstV1I GCAGC 4 cut(s) 925, 1001, 1338, 1444
BsuI GTATCC 1 cut(s) 1280
BsuRI GGCC 5 cut(s) 127, 189, 300, 314, 846
BtgZI GCGATG 1 cut(s) 115
BtrI CACGTC 2 cut(s) 445, 980
BtsCI GGATG 4 cut(s) 79, 442, 1211, 1419
BtsI GCAGTG 2 cut(s) 11, 30
BtsIMutI CAGTG 2 cut(s) 11, 30
Cac8I GCNNGC 3 cut(s) 558, 646, 882
CaiI CAGNNNCTG 2 cut(s) 1027, 1216
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 4 cut(s) 125, 126, 312, 1408
CpoI CGGWCCG 1 cut(s) 1408
CseI GACGC 3 cut(s) 16, 298, 1156
Csp6I GTAC 4 cut(s) 587, 863, 1090, 1364
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CspI CGGWCCG 1 cut(s) 1408
CviQI GTAC 4 cut(s) 587, 863, 1090, 1364
DdeI CTNAG 1 cut(s) 851
DpnI GATC 3 cut(s) 352, 359, 1058
DpnII GATC 3 cut(s) 350, 357, 1056
DraIII CACNNNGTG 1 cut(s) 25
DriI GACNNNNNGTC 1 cut(s) 1017
EaeI YGGCCR 2 cut(s) 187, 298
Eam1104I CTCTTC 2 cut(s) 614, 1344
Eam1105I GACNNNNNGTC 1 cut(s) 1017
EarI CTCTTC 2 cut(s) 614, 1344
EciI GGCGGA 4 cut(s) 202, 397, 778, 1218
Eco24I GRGCYC 2 cut(s) 129, 1260
Eco31I GGTCTC 1 cut(s) 961
Eco47I GGWCC 1 cut(s) 1408
Eco57I CTGAAG 2 cut(s) 52, 515
Eco88I CYCGRG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 804
EcoT38I GRGCYC 2 cut(s) 129, 1260
Esp3I CGTCTC 1 cut(s) 281
FaqI GGGAC 2 cut(s) 1227, 1345
FauI CCCGC 2 cut(s) 1269, 1322
FauNDI CATATG 1 cut(s) 758
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 5 cut(s) 34, 939, 990, 1352, 1458
FokI GGATG 4 cut(s) 86, 449, 1218, 1426
FriOI GRGCYC 2 cut(s) 129, 1260
Fsp4HI GCNGC 5 cut(s) 34, 939, 990, 1352, 1458
FspBI CTAG 1 cut(s) 1130
GluI GCNGC 5 cut(s) 34, 939, 990, 1352, 1458
GsuI CTGGAG 2 cut(s) 188, 827
HaeIII GGCC 5 cut(s) 127, 189, 300, 314, 846
HapII CCGG 5 cut(s) 56, 315, 772, 1185, 1411
HgaI GACGC 3 cut(s) 16, 298, 1156
Hin1I GRCGYC 1 cut(s) 8
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 2 cut(s) 303, 422
HinfI GANTC 5 cut(s) 785, 826, 875, 930, 1038
HpaI GTTAAC 1 cut(s) 325
HpaII CCGG 5 cut(s) 56, 315, 772, 1185, 1411
HphI GGTGA 3 cut(s) 70, 361, 1454
Hpy166II GTNNAC 9 cut(s) 28, 325, 340, 369, 726, 1006, 1092, 1231, 1425
Hpy188I TCNGA 4 cut(s) 197, 1037, 1145, 1199
Hpy188III TCNNGA 1 cut(s) 1060
Hpy8I GTNNAC 9 cut(s) 28, 325, 340, 369, 726, 1006, 1092, 1231, 1425
Hpy99I CGWCG 1 cut(s) 1103
HpyAV CCTTC 6 cut(s) 145, 256, 514, 539, 713, 1283
HpyCH4III ACNGT 3 cut(s) 905, 1015, 1442
HpyCH4IV ACGT 4 cut(s) 444, 618, 979, 1008
HpyCH4V TGCA 9 cut(s) 122, 150, 243, 346, 480, 644, 653, 714, 1111
HpyF10VI GCNNNNNNNGC 6 cut(s) 156, 311, 430, 455, 650, 1326
HpyF3I CTNAG 1 cut(s) 851
HpySE526I ACGT 4 cut(s) 444, 618, 979, 1008
Hsp92I GRCGYC 1 cut(s) 8
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 3 cut(s) 350, 357, 1056
LguI GCTCTTC 1 cut(s) 1344
LmnI GCTCC 1 cut(s) 1263
Lsp1109I GCAGC 4 cut(s) 925, 1001, 1338, 1444
LweI GCATC 3 cut(s) 64, 669, 786
MaeI CTAG 1 cut(s) 1130
MaeII ACGT 4 cut(s) 444, 618, 979, 1008
MaeIII GTNAC 4 cut(s) 1009, 1147, 1241, 1442
MalI GATC 3 cut(s) 352, 359, 1058
MbiI CCGCTC 1 cut(s) 1264
MboI GATC 3 cut(s) 350, 357, 1056
MboII GAAGA 6 cut(s) 127, 394, 601, 1130, 1331, 1429
MhlI GDGCHC 2 cut(s) 129, 1260
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 6 cut(s) 591, 675, 895, 1106, 1134, 1308
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 3 cut(s) 794, 820, 869
MmeI TCCRAC 2 cut(s) 984, 1195
MnlI CCTC 8 cut(s) 54, 212, 395, 1096, 1193, 1310, 1384, 1441
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MseI TTAA 2 cut(s) 324, 893
MslI CAYNNNNRTG 2 cut(s) 617, 1052
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 5 cut(s) 56, 315, 772, 1185, 1411
MspR9I CCNGG 1 cut(s) 806
MvaI CCWGG 1 cut(s) 806
MwoI GCNNNNNNNGC 6 cut(s) 156, 311, 430, 455, 650, 1326
NdeI CATATG 1 cut(s) 758
NdeII GATC 3 cut(s) 350, 357, 1056
NlaIV GGNNCC 3 cut(s) 127, 313, 1259
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 3 cut(s) 1009, 1147, 1442
NspI RCATGY 3 cut(s) 323, 331, 346
PciI ACATGT 1 cut(s) 319
PciSI GCTCTTC 1 cut(s) 1344
PcsI WCGNNNNNNNCGW 1 cut(s) 743
PfeI GAWTC 2 cut(s) 930, 1038
PkrI GCNGC 5 cut(s) 35, 940, 991, 1353, 1459
PleI GAGTC 3 cut(s) 793, 820, 869
PpsI GAGTC 3 cut(s) 793, 820, 869
Ppu21I YACGTR 2 cut(s) 619, 1009
PscI ACATGT 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 804
PspGI CCWGG 1 cut(s) 804
PspN4I GGNNCC 3 cut(s) 127, 313, 1259
PspOMI GGGCCC 1 cut(s) 125
PspPI GGNCC 4 cut(s) 125, 126, 312, 1408
PstNI CAGNNNCTG 2 cut(s) 1027, 1216
PvuII CAGCTG 1 cut(s) 375
RsaI GTAC 4 cut(s) 588, 864, 1091, 1365
RsaNI GTAC 4 cut(s) 587, 863, 1090, 1364
RseI CAYNNNNRTG 2 cut(s) 617, 1052
Rsr2I CGGWCCG 1 cut(s) 1408
RsrII CGGWCCG 1 cut(s) 1408
SapI GCTCTTC 1 cut(s) 1344
SaqAI TTAA 2 cut(s) 324, 893
SatI GCNGC 5 cut(s) 34, 939, 990, 1352, 1458
Sau3AI GATC 3 cut(s) 350, 357, 1056
Sau96I GGNCC 4 cut(s) 125, 126, 312, 1408
SchI GAGTC 3 cut(s) 794, 820, 869
ScrFI CCNGG 1 cut(s) 806
SduI GDGCHC 2 cut(s) 129, 1260
SfaNI GCATC 3 cut(s) 64, 669, 786
SfcI CTRYAG 1 cut(s) 840
SinI GGWCC 1 cut(s) 1408
SmiMI CAYNNNNRTG 2 cut(s) 617, 1052
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 6 cut(s) 591, 675, 895, 1106, 1134, 1308
SsiI CCGC 8 cut(s) 33, 213, 408, 485, 789, 1203, 1262, 1329
SspI AATATT 2 cut(s) 694, 998
SspMI CTAG 1 cut(s) 1130
StyD4I CCNGG 1 cut(s) 804
TaaI ACNGT 3 cut(s) 905, 1015, 1442
TaiI ACGT 4 cut(s) 447, 621, 982, 1011
TaqI TCGA 1 cut(s) 624
TaqII GACCGA 1 cut(s) 69
TasI AATT 6 cut(s) 591, 675, 895, 1106, 1134, 1308
TatI WGTACW 3 cut(s) 586, 1089, 1363
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 2 cut(s) 930, 1038
Tru1I TTAA 2 cut(s) 324, 893
Tru9I TTAA 2 cut(s) 324, 893
TscAI CASTG 2 cut(s) 18, 30
TseFI GTSAC 3 cut(s) 1009, 1147, 1442
TseI GCWGC 4 cut(s) 938, 989, 1351, 1457
Tsp45I GTSAC 3 cut(s) 1009, 1147, 1442
TspDTI ATGAA 7 cut(s) 111, 250, 601, 719, 913, 1036, 1430
TspGWI ACGGA 2 cut(s) 223, 1421
TspRI CASTG 2 cut(s) 18, 30
VpaK11BI GGWCC 1 cut(s) 1408
XapI RAATTY 2 cut(s) 895, 1308
XceI RCATGY 3 cut(s) 323, 331, 346
XmiI GTMKAC 1 cut(s) 1005
XspI CTAG 1 cut(s) 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.