FvH4_7g30080

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000970
Physical Location & Seq
Reverse (-)
21995481 .. 21998809
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30080.t3

Sequence Viewer

Length: 1506 bp
ATGGAGGAGCCATTACTCGATGCAGCTGCTGCGAATTCTGAGTTCAACCACAAGGCTTTGTTGGGTGATGGCGACGAAGACTACAAGCCGGTGAGGAGCTTTCCGGAGTTTCGGTCTATGTTTTGGATAGAGACGGTGAAGCTGTGGAAAATAGCAGGTCCTTCAGTGATTGGAATCCTGGCCATGTATGGGACTAACGCCATCATCAGCTTGTTTGCTGGTCATATTGGAAGTATTGAGCTCTCAGCAATCTCCATATCTCTCTCCGTCATTTCCACCTTCTCTTATGGATTCTTGCTCGGCATGGGAAGTGCTTTGGAGACCTTGTGTGGACAGGCATTTGGTGCAGGACAGATTCATATGCTTGGCATTTACATGCAGCGCTCATGTATAGTCCTACTTGTAACATGCTTCCTCCTGTTGCCAGTTTACATCTTTGCCACTCCTATTTTAAAGTTCTTAGGCCAAGAAGATGACATAGCCGATCTCGCAGGAGAGTTCACACTGCAAATAATTCCTCAGTTGTTCTCACTTGCCATTAACTTTCCTGCTCAGAAGTTCCTTCAGGCTCAGAGAAAGCTCAAAGTCATGACCTGGATTTCTGTGGTAGCGTTCATCATACATGTTGGCTTACTCTTCTTCTTCATTTTTGGGTTAGGATGGGGTACCTTAGGTGCAGCTGTGGCATATAATATTACCAGATGGGGCATCGCAATTGCTCAAGTTGTGTATATTTCAGTCTGGTGCAATGAGGGTTGGACTGGGTTTTCATGGTTGGCATTCAAGGATATGTGGTCTTTTGTTAGGCTTTCGATTGCATCTGCTGTCATGCTTTGCCTTGAGATATGGTATTTCATGAGTATGATGCTTCTCACTGGAAACCTAAGCGATGCAGTTCTAGCAGTTAGTTCCCTTTCTATTTGCCTCAACATTAACGGGTGGGAAGTAATGCTGTTTGTTGGAATAAATATTGCTATAAGTGTTCGAGTATCGAACGAGCTTGGTTTAGGACGCCCAAGAGCGGCCAAATATGCAGTCTATGTGACAGTATTTCAGTCTTTCCTAATGGGGATATTTTTCATGATTCTGATCTTGTTAACCAAAAATAGCTTCTCTGTGCTTTTTACTACTGACAAGGAGCTGCAACAAGCGGTTGCGCATCTAGCTTACCTTCTCGGCGCAACTATGCTGCTCAACAGTGTCCAGCCAGTAATATCAGGTGTTGCCATTGGCGGTGGATGGCAGGCAACTGTGGCGTATATAAACTTGGGTTGTTACTACATTTTTGGAATTCCTCTCGGATACTTGCTTAGTCACGTTGCAGATTTCGGAGTGATGGGACTTTGGGGCGGTATGATATGTGGAACTGCTCTTCAGACAATACTTCTTTTAATTGTGCTTTACAGAACTAACTGGAACAAAGAGGTAGAGCAAGCAACAACACGCGTGCAGAAGTGGGGTTTAGAAGACCGCAAAACTGAGACTATGGCTGAAAGTGCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

502

Amino Acids

55.19

Weight (kDa)

5.74

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 54 - 211 9.3e-31 MatE
MatE PF01554 274 - 436 1.1e-25 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1158
Acc36I ACCTGC 1 cut(s) 146
Acc65I GGTACC 1 cut(s) 665
AccB1I GGYRCC 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 1022
AccII CGCG 1 cut(s) 1446
AccIII TCCGGA 1 cut(s) 103
AciI CCGC 5 cut(s) 1022, 1151, 1233, 1350, 1471
AcoI YGGCCR 2 cut(s) 180, 1023
AcsI RAATTY 2 cut(s) 34, 1290
AcuI CTGAAG 3 cut(s) 147, 548, 1358
AcyI GRCGYC 1 cut(s) 1012
AfaI GTAC 1 cut(s) 667
AfeI AGCGCT 1 cut(s) 383
AfiI CCNNNNNNNGG 2 cut(s) 189, 1021
AflIII ACRYGT 2 cut(s) 622, 1444
AgsI TTSAA 2 cut(s) 46, 784
AjnI CCWGG 2 cut(s) 177, 593
AjuI GAANNNNNNNTTGG 2 cut(s) 1094, 1126
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 3 cut(s) 125, 314, 1475
AlwNI CAGNNNCTG 1 cut(s) 29
Aor13HI TCCGGA 1 cut(s) 103
Aor51HI AGCGCT 1 cut(s) 383
AoxI GGCC 3 cut(s) 180, 463, 1023
ApeKI GCWGC 7 cut(s) 23, 26, 29, 379, 677, 1141, 1189
ApoI RAATTY 2 cut(s) 34, 1290
ArsI GACNNNNNNTTYG 2 cut(s) 1020, 1052
Asp718I GGTACC 1 cut(s) 665
AspLEI GCGC 3 cut(s) 384, 1159, 1181
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 77, 103, 148
AvaII GGWCC 1 cut(s) 158
AxyI CCTNAGG 1 cut(s) 670
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 2 cut(s) 84, 1473
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 7 cut(s) 13, 16, 35, 391, 689, 1128, 1176
BccI CCATC 6 cut(s) 62, 209, 654, 696, 1233, 1330
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BciT130I CCWGG 2 cut(s) 179, 595
BciVI GTATCC 1 cut(s) 1295
BcoDI GTCTC 3 cut(s) 125, 314, 1475
BfaI CTAG 2 cut(s) 899, 1163
BfoI RGCGCY 1 cut(s) 385
BfuAI ACCTGC 1 cut(s) 146
BfuI GTATCC 1 cut(s) 1295
BisI GCNGC 8 cut(s) 24, 27, 30, 380, 678, 1023, 1142, 1190
BlsI GCNGC 8 cut(s) 25, 28, 31, 381, 679, 1024, 1143, 1191
Bme1390I CCNGG 2 cut(s) 179, 595
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 2 cut(s) 9, 667
BmrFI CCNGG 2 cut(s) 179, 595
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 6 cut(s) 10, 717, 827, 855, 880, 1168
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 2 cut(s) 84, 1473
Bpu10I CCTNAGC 1 cut(s) 884
BpuEI CTTGAG 2 cut(s) 705, 860
BsaBI GATNNNNATC 2 cut(s) 173, 1088
BsaHI GRCGYC 1 cut(s) 1012
BsaI GGTCTC 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 103
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 311, 341
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 1021
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 5 cut(s) 425, 766, 880, 1208, 1418
Bse21I CCTNAGG 1 cut(s) 670
Bse3DI GCAATG 1 cut(s) 754
Bse8I GATNNNNATC 2 cut(s) 173, 1088
BseAI TCCGGA 1 cut(s) 103
BseBI CCWGG 2 cut(s) 179, 595
BseGI GGATG 2 cut(s) 665, 1244
BseJI GATNNNNATC 2 cut(s) 173, 1088
BseLI CCNNNNNNNGG 2 cut(s) 189, 1021
BseMI GCAATG 1 cut(s) 754
BseMII CTCAG 6 cut(s) 30, 258, 533, 566, 584, 1470
BseNI ACTGG 5 cut(s) 425, 766, 880, 1208, 1418
BseRI GAGGAG 2 cut(s) 20, 109
BseXI GCAGC 7 cut(s) 13, 16, 35, 391, 689, 1128, 1176
BsgI GTGCAG 3 cut(s) 366, 696, 1469
Bsh1236I CGCG 1 cut(s) 1446
BshFI GGCC 3 cut(s) 182, 465, 1025
BshNI GGYRCC 1 cut(s) 665
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 2 cut(s) 89, 104
BslFI GGGAC 2 cut(s) 205, 1353
BslI CCNNNNNNNGG 2 cut(s) 189, 1021
BsmAI GTCTC 3 cut(s) 125, 314, 1475
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 2 cut(s) 205, 1353
BsmI GAATGC 1 cut(s) 779
BsnI GGCC 3 cut(s) 182, 465, 1025
Bso31I GGTCTC 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 243
Bsp13I TCCGGA 1 cut(s) 103
Bsp143I GATC 2 cut(s) 484, 1089
BspACI CCGC 5 cut(s) 1022, 1151, 1233, 1350, 1471
BspANI GGCC 3 cut(s) 182, 465, 1025
BspCNI CTCAG 6 cut(s) 31, 257, 532, 565, 583, 1471
BspEI TCCGGA 1 cut(s) 103
BspFNI CGCG 1 cut(s) 1446
BspHI TCATGA 3 cut(s) 588, 855, 1080
BspLI GGNNCC 2 cut(s) 9, 667
BspMI ACCTGC 1 cut(s) 146
BspQI GCTCTTC 1 cut(s) 1377
BspT107I GGYRCC 1 cut(s) 665
BspTNI GGTCTC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 1022
BsrDI GCAATG 1 cut(s) 754
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 5 cut(s) 425, 766, 880, 1208, 1418
BssAI RCCGGY 1 cut(s) 88
BssMI GATC 2 cut(s) 484, 1089
BssNI GRCGYC 1 cut(s) 1012
Bst2UI CCWGG 2 cut(s) 179, 595
Bst4CI ACNGT 4 cut(s) 136, 1048, 1199, 1252
Bst6I CTCTTC 2 cut(s) 641, 1377
BstACI GRCGYC 1 cut(s) 1012
BstAPI GCANNNNNTGC 2 cut(s) 29, 344
BstC8I GCNNGC 3 cut(s) 1245, 1434, 1448
BstF5I GGATG 2 cut(s) 665, 1244
BstFNI CGCG 1 cut(s) 1446
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 3 cut(s) 384, 1159, 1181
BstKTI GATC 2 cut(s) 487, 1092
BstMAI GTCTC 3 cut(s) 125, 314, 1475
BstMBI GATC 2 cut(s) 484, 1089
BstMWI GCNNNNNNNGC 9 cut(s) 29, 344, 488, 683, 899, 1031, 1163, 1253, 1496
BstNI CCWGG 2 cut(s) 179, 595
BstNSI RCATGY 3 cut(s) 379, 411, 626
BstSCI CCNGG 2 cut(s) 177, 593
BstUI CGCG 1 cut(s) 1446
BstV1I GCAGC 7 cut(s) 13, 16, 35, 391, 689, 1128, 1176
BstV2I GAAGAC 2 cut(s) 84, 1473
Bsu36I CCTNAGG 1 cut(s) 670
BsuI GTATCC 1 cut(s) 1295
BsuRI GGCC 3 cut(s) 182, 465, 1025
BtgZI GCGATG 2 cut(s) 694, 903
BtsCI GGATG 2 cut(s) 665, 1244
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 4 cut(s) 171, 503, 873, 1204
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 3 cut(s) 1245, 1434, 1448
CaiI CAGNNNCTG 1 cut(s) 29
CciI TCATGA 3 cut(s) 588, 855, 1080
CfoI GCGC 3 cut(s) 384, 1159, 1181
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 1020
Csp6I GTAC 1 cut(s) 666
CviQI GTAC 1 cut(s) 666
DpnI GATC 2 cut(s) 486, 1091
DpnII GATC 2 cut(s) 484, 1089
DraI TTTAAA 1 cut(s) 453
EaeI YGGCCR 2 cut(s) 180, 1023
Eam1104I CTCTTC 2 cut(s) 641, 1377
EarI CTCTTC 2 cut(s) 641, 1377
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 158
Eco47III AGCGCT 1 cut(s) 383
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 3 cut(s) 147, 548, 1358
Eco81I CCTNAGG 1 cut(s) 670
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 2 cut(s) 34, 1290
EcoRII CCWGG 2 cut(s) 177, 593
EcoT38I GRGCYC 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 125
FaqI GGGAC 2 cut(s) 205, 1353
FauNDI CATATG 1 cut(s) 360
Fnu4HI GCNGC 8 cut(s) 24, 27, 30, 380, 678, 1023, 1142, 1190
FokI GGATG 2 cut(s) 672, 1251
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 8 cut(s) 24, 27, 30, 380, 678, 1023, 1142, 1190
FspBI CTAG 2 cut(s) 899, 1163
FspI TGCGCA 1 cut(s) 1158
GlaI GCGC 3 cut(s) 383, 1158, 1180
GluI GCNGC 8 cut(s) 24, 27, 30, 380, 678, 1023, 1142, 1190
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 3 cut(s) 182, 465, 1025
HapII CCGG 2 cut(s) 89, 104
HgaI GACGC 1 cut(s) 1020
HhaI GCGC 3 cut(s) 384, 1159, 1181
Hin1I GRCGYC 1 cut(s) 1012
Hin6I GCGC 3 cut(s) 382, 1157, 1179
HinP1I GCGC 3 cut(s) 382, 1157, 1179
HincII GTYRAC 1 cut(s) 1098
HindII GTYRAC 1 cut(s) 1098
HinfI GANTC 4 cut(s) 174, 291, 355, 1084
HpaI GTTAAC 1 cut(s) 1098
HpaII CCGG 2 cut(s) 89, 104
HphI GGTGA 3 cut(s) 77, 103, 148
Hpy166II GTNNAC 4 cut(s) 332, 430, 501, 1098
Hpy188I TCNGA 7 cut(s) 40, 555, 573, 1089, 1301, 1331, 1377
Hpy188III TCNNGA 4 cut(s) 104, 589, 856, 1081
Hpy8I GTNNAC 4 cut(s) 332, 430, 501, 1098
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 4 cut(s) 171, 289, 572, 1181
HpyCH4III ACNGT 4 cut(s) 136, 1048, 1199, 1252
HpyCH4IV ACGT 1 cut(s) 1317
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 344, 488, 683, 899, 1031, 1163, 1253, 1496
HpySE526I ACGT 1 cut(s) 1317
Hsp92I GRCGYC 1 cut(s) 1012
HspAI GCGC 3 cut(s) 382, 1157, 1179
Kpn2I TCCGGA 1 cut(s) 103
KpnI GGTACC 1 cut(s) 669
KspAI GTTAAC 1 cut(s) 1098
Kzo9I GATC 2 cut(s) 484, 1089
LguI GCTCTTC 1 cut(s) 1377
LmnI GCTCC 3 cut(s) 7, 96, 1138
Lsp1109I GCAGC 7 cut(s) 13, 16, 35, 391, 689, 1128, 1176
LweI GCATC 6 cut(s) 10, 717, 827, 855, 880, 1168
MaeI CTAG 2 cut(s) 899, 1163
MaeII ACGT 1 cut(s) 1317
MaeIII GTNAC 4 cut(s) 403, 1042, 1274, 1313
MalI GATC 2 cut(s) 486, 1091
MbiI CCGCTC 1 cut(s) 1022
MboI GATC 2 cut(s) 484, 1089
MboII GAAGA 7 cut(s) 89, 482, 628, 631, 634, 1364, 1478
MfeI CAATTG 1 cut(s) 714
MhlI GDGCHC 1 cut(s) 243
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 5 cut(s) 34, 513, 714, 1290, 1392
MluI ACGCGT 1 cut(s) 1444
MluNI TGGCCA 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 737, 940
MnlI CCTC 7 cut(s) 87, 425, 528, 745, 935, 1305, 1417
Mox20I TGGCCA 1 cut(s) 182
MroI TCCGGA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 182
MseI TTAA 5 cut(s) 452, 540, 933, 1097, 1391
MslI CAYNNNNRTG 2 cut(s) 374, 860
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 2 cut(s) 26, 680
MspI CCGG 2 cut(s) 89, 104
MspR9I CCNGG 2 cut(s) 179, 595
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 779
MvaI CCWGG 2 cut(s) 179, 595
MvnI CGCG 1 cut(s) 1446
MwoI GCNNNNNNNGC 9 cut(s) 29, 344, 488, 683, 899, 1031, 1163, 1253, 1496
NdeI CATATG 1 cut(s) 360
NdeII GATC 2 cut(s) 484, 1089
NlaIV GGNNCC 2 cut(s) 9, 667
NmeAIII GCCGAG 2 cut(s) 279, 1155
NmuCI GTSAC 2 cut(s) 1042, 1313
NsbI TGCGCA 1 cut(s) 1158
NspI RCATGY 3 cut(s) 379, 411, 626
PagI TCATGA 3 cut(s) 588, 855, 1080
PciI ACATGT 1 cut(s) 622
PciSI GCTCTTC 1 cut(s) 1377
PctI GAATGC 1 cut(s) 779
PfeI GAWTC 4 cut(s) 174, 291, 355, 1084
PkrI GCNGC 8 cut(s) 25, 28, 31, 381, 679, 1024, 1143, 1191
PpuMI RGGWCCY 1 cut(s) 158
PscI ACATGT 1 cut(s) 622
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 177, 593
PspGI CCWGG 2 cut(s) 177, 593
PspN4I GGNNCC 2 cut(s) 9, 667
PspPI GGNCC 1 cut(s) 158
PspPPI RGGWCCY 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 2 cut(s) 26, 680
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
RseI CAYNNNNRTG 2 cut(s) 374, 860
SacI GAGCTC 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 1377
SaqAI TTAA 5 cut(s) 452, 540, 933, 1097, 1391
SatI GCNGC 8 cut(s) 24, 27, 30, 380, 678, 1023, 1142, 1190
Sau3AI GATC 2 cut(s) 484, 1089
Sau96I GGNCC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 179, 595
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 6 cut(s) 10, 717, 827, 855, 880, 1168
SinI GGWCC 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 374, 860
SmlI CTYRAG 2 cut(s) 720, 839
SmoI CTYRAG 2 cut(s) 720, 839
Sse9I AATT 5 cut(s) 34, 513, 714, 1290, 1392
SsiI CCGC 5 cut(s) 1022, 1151, 1233, 1350, 1471
SspI AATATT 2 cut(s) 694, 970
SspMI CTAG 2 cut(s) 899, 1163
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 177, 593
TaaI ACNGT 4 cut(s) 136, 1048, 1199, 1252
TaiI ACGT 1 cut(s) 1320
TaqI TCGA 4 cut(s) 18, 812, 985, 992
TaqII GACCGA 1 cut(s) 102
TasI AATT 5 cut(s) 34, 513, 714, 1290, 1392
TauI GCSGC 1 cut(s) 1025
TfiI GAWTC 4 cut(s) 174, 291, 355, 1084
Tru1I TTAA 5 cut(s) 452, 540, 933, 1097, 1391
Tru9I TTAA 5 cut(s) 452, 540, 933, 1097, 1391
TscAI CASTG 4 cut(s) 171, 510, 880, 1204
TseFI GTSAC 2 cut(s) 1042, 1313
TseI GCWGC 7 cut(s) 23, 26, 29, 379, 677, 1141, 1189
Tsp45I GTSAC 2 cut(s) 1042, 1313
TspDTI ATGAA 6 cut(s) 347, 604, 634, 759, 844, 1069
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 4 cut(s) 171, 510, 880, 1204
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 2 cut(s) 34, 1290
XceI RCATGY 3 cut(s) 379, 411, 626
XspI CTAG 2 cut(s) 899, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.