pycom01g21640

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000970
Physical Location & Seq
Reverse (-)
19581192 .. 19586937
5746 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21640.7

Sequence Viewer

Length: 2127 bp
ATGTTCACGATAGTTTTAGAGGAGCCGCTGCTTCACACAGCTGCTGCCGGCGCCGATGAGTTGAGCAATAAACCTCCTCTTTATGAAGGCGGGAACGAAGACTATGCACCGGTGAGGAGCTTTGATGCGTTCCAGCGTATGTTTTGGATAGAGACGGCAAAGCTGTGGCAAATAGGCGTTTCGTCAGTGATCACTATCATCTGTATGTATGGGACTAACGCAGTCATCCTGCTCTTTGCCGGTCATCTCGGAACTATCCAGATCTCCGCCATCTCGATTTCTCTGGCTGTCATTTCCACATTCACTGATGGATTGATGGTACTTAATCTCCTTCCCCTTGCCCTGGAGACCTTGTGCGGGCAGGCGTTTGGCGCTGGACAAGTTCATATGCTTGGGATCTACCTGCAACGATCATGGATAATTCTATTTGTAACCACCCTCTTCCTTTTGCCGATTTACATCTTCGCCGTGCCGATTTTAAAGTGGCTAGGCCAAGAAGACGACATAGCAAACCTAGCCGGAAAATTCACACTCCAAATAATCCCCCAAATATTCTCACTTGCCATATATTTCCCAGCTCAGAAGTTCCTCCAAGCTCAGAGAAAAGTAAAGGTGCTGGCATGGATTGCATTTTTAGGTCTGGTCATACACATTGGAATGCTAGGGCTCTTCATGTATGAGTTTGATTGGGGCACATTGGGCGCAGCTGTGTCATTTAACATCACAAGATGGGGGATTGCAATTGCTCAAGTTGTGTATATTATGGGTTGGTGCAAAGAGGGTTGGACTGGATTTTCATGGCTCGCTTTCAAGGACATTTGGGAATTTGTAAGACTTTCTCTTGCCTCTGCAGTCATGCTCTGCCTTGAGATTTGGTACATGATGAGTATACTCATTCTCACCGGCAGTCTTTCTAACGCTGTTATAGCCGTTGGTTCTCTTTCTGTTTGCATGAACATAAACGGGTGGGAAGCAATGTTGTTCGTTGGAATAAATATTTCAACAAGTATTCGTGTCTCTAATGAACTCGGGTTAGGACGTCCCAGAGCAACAAAATACTCTATCTATGTGACAGTATTTCAATCTTTCTTGATCGGAATTCTTTTCATGATTATTATCTTAATGACTAGAGATGATTTCGCTGTGCTTTTCACAAGTGTTGCAATTGGAGGTGGATGGCAGACAATGGTTGCCTATCTAAACTTGGGTTGTTACTACATTTTTGGAATTCCTCTTGGATACCTTCTTTGCCATGTTGCAAAATGGGGAGTCATGGGACTTTGGGGAGGCATGATATGTGGAACTGCTCTCCAAACAATACTTCTGCTAATTATATTGTACAAAACTAACTGGAACAAAGAGGTAATTAATCAATACTTTATTTCAAATCGCTTCAACTTTGGTTTCTGCATTGTGGAGCAAGCAAAAAATCGCGTCCATAAGTGGGGTGGGCAAGACATCAAAACCGAGAGTGGAGCTGAACTAACCCTTTTGAATCTCTCAATGGAAACACCATTGCTGAAAATCAATGCAGAGGCGGCAGCAGCTGCTGATTATGCTCCAGTGAGGAGCTTTGGGGAGGCCATGGAGGTCTCTAGGAAAGAGGCTGTGGAGAATTGCAGCTCCGGTGGCCTGCACTTCTCTTTTTCAATACCTGATTCAGTCCATAACCACCGTCTTCGTCGGTCATCTCGGAGATGTCGAGCTCACTGCTGTCTCTCTCTCCCTCAGTCAAGGTATGTCAACTGCATTGGGAACCCTTGGAGCAGGCCAAGTAGGGAAGCTAGGAATCTATATGCAGTTCTCATGGATTATGTTATTCATCATCTGCATCATCCTTATGCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCCGATCCTAAAATTTTTAGGCCAAGAACATGGCATAGCGAATCTTTCTGAAGTATACAGCCTAAAGACAATCCCTCAAATGTTTTCCTATGCTATCAATTTGCCCACTAAGAGGTTCCTCCAGGTTCAGAGCAAGGTTATCGTGATCACGCTGATCGCATTCGTGGCTCTGGTCATACAAACCTGGCTACTTATCTCTTCATCAATGTCTTTGGCTGGGGAGCAACTGGTGCAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

709

Amino Acids

79.42

Weight (kDa)

8.42

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1042
Acc36I ACCTGC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 50
AccI GTMKAC 2 cut(s) 889, 1941
AccII CGCG 1 cut(s) 1434
AciI CCGC 5 cut(s) 26, 90, 267, 357, 1538
AclWI GGATC 2 cut(s) 404, 1885
AcsI RAATTY 5 cut(s) 524, 824, 1098, 1227, 1898
AcuI CTGAAG 1 cut(s) 1956
AcyI GRCGYC 2 cut(s) 51, 1039
AfaI GTAC 3 cut(s) 321, 878, 1340
AfiI CCNNNNNNNGG 4 cut(s) 343, 357, 1444, 1998
AgeI ACCGGT 1 cut(s) 109
AgsI TTSAA 7 cut(s) 811, 1002, 1082, 1386, 1396, 1495, 1650
AjnI CCWGG 3 cut(s) 342, 2007, 2069
AjuI GAANNNNNNNTTGG 2 cut(s) 1305, 1337
Alw21I GWGCWC 1 cut(s) 1708
Alw26I GTCTC 5 cut(s) 146, 341, 1021, 1597, 1721
AlwI GGATC 2 cut(s) 404, 1885
AlwNI CAGNNNCTG 3 cut(s) 44, 1547, 1550
Ama87I CYCGRG 1 cut(s) 1028
AoxI GGCC 5 cut(s) 490, 1581, 1630, 1769, 1906
ApeKI GCWGC 9 cut(s) 28, 41, 44, 704, 1541, 1544, 1547, 1620, 2119
ApoI RAATTY 5 cut(s) 524, 824, 1098, 1227, 1898
AseI ATTAAT 1 cut(s) 1368
AsiGI ACCGGT 1 cut(s) 109
AspLEI GCGC 3 cut(s) 53, 374, 704
AsuHPI GGTGA 2 cut(s) 124, 892
AvaI CYCGRG 1 cut(s) 1028
BaeGI GKGCMC 1 cut(s) 695
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 2 cut(s) 669, 1708
BbsI GAAGAC 3 cut(s) 105, 504, 1670
Bbv12I GWGCWC 1 cut(s) 1708
BbvI GCAGC 8 cut(s) 15, 28, 31, 716, 1534, 1553, 1556, 1632
BccI CCATC 5 cut(s) 278, 302, 310, 723, 1170
BceAI ACGGC 3 cut(s) 171, 452, 914
BcgI CGANNNNNNTGC 6 cut(s) 86, 120, 1692, 1726, 2008, 2042
BciT130I CCWGG 3 cut(s) 344, 2009, 2071
BciVI GTATCC 1 cut(s) 1232
BclI TGATCA 2 cut(s) 189, 2031
BcoDI GTCTC 5 cut(s) 146, 341, 1021, 1597, 1721
BfaI CTAG 6 cut(s) 488, 515, 662, 1128, 1596, 1785
BfmI CTRYAG 2 cut(s) 849, 2123
BfoI RGCGCY 2 cut(s) 54, 375
BfuAI ACCTGC 1 cut(s) 411
BfuI GTATCC 1 cut(s) 1232
BglII AGATCT 1 cut(s) 261
Bme1390I CCNGG 3 cut(s) 344, 2009, 2071
BmeT110I CYCGRG 1 cut(s) 1028
BmiI GGNNCC 4 cut(s) 24, 52, 1757, 2003
BmrFI CCNGG 3 cut(s) 344, 2009, 2071
BmsI GCATC 2 cut(s) 115, 1840
BpiI GAAGAC 3 cut(s) 105, 504, 1670
BpmI CTGGAG 3 cut(s) 365, 1545, 1991
BpuEI CTTGAG 2 cut(s) 732, 887
BsaBI GATNNNNATC 3 cut(s) 194, 458, 1115
BsaHI GRCGYC 2 cut(s) 51, 1039
BsaI GGTCTC 2 cut(s) 341, 1597
BsaJI CCNNGG 3 cut(s) 342, 1584, 1760
BsaWI WCCGGW 2 cut(s) 109, 1625
BsaXI ACNNNNNCTCC 4 cut(s) 312, 338, 342, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 343, 357, 1444, 1998
Bse118I RCCGGY 4 cut(s) 47, 109, 239, 902
Bse1I ACTGG 4 cut(s) 793, 1355, 1562, 2118
Bse3DI GCAATG 2 cut(s) 981, 1514
Bse8I GATNNNNATC 3 cut(s) 194, 458, 1115
BseBI CCWGG 3 cut(s) 344, 2009, 2071
BseDI CCNNGG 3 cut(s) 342, 1584, 1760
BseGI GGATG 3 cut(s) 225, 1181, 1834
BseJI GATNNNNATC 3 cut(s) 194, 458, 1115
BseLI CCNNNNNNNGG 4 cut(s) 343, 357, 1444, 1998
BseMI GCAATG 2 cut(s) 981, 1514
BseMII CTCAG 3 cut(s) 593, 611, 1742
BseNI ACTGG 4 cut(s) 793, 1355, 1562, 2118
BseRI GAGGAG 4 cut(s) 35, 66, 130, 1582
BseSI GKGCMC 1 cut(s) 695
BseXI GCAGC 8 cut(s) 15, 28, 31, 716, 1534, 1553, 1556, 1632
BseYI CCCAGC 2 cut(s) 574, 2102
BsgI GTGCAG 1 cut(s) 1619
Bsh1236I CGCG 1 cut(s) 1434
BshFI GGCC 5 cut(s) 492, 1583, 1632, 1771, 1908
BshNI GGYRCC 1 cut(s) 50
BshTI ACCGGT 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 1708
BsiHKCI CYCGRG 1 cut(s) 1028
BsiSI CCGG 6 cut(s) 48, 110, 240, 519, 903, 1626
BslFI GGGAC 3 cut(s) 226, 1026, 1290
BslI CCNNNNNNNGG 4 cut(s) 343, 357, 1444, 1998
BsmAI GTCTC 5 cut(s) 146, 341, 1021, 1597, 1721
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 3 cut(s) 226, 1026, 1290
BsmI GAATGC 2 cut(s) 663, 2045
BsnI GGCC 5 cut(s) 492, 1583, 1632, 1771, 1908
Bso31I GGTCTC 2 cut(s) 341, 1597
BsoBI CYCGRG 1 cut(s) 1028
Bsp1286I GDGCHC 3 cut(s) 669, 695, 1708
Bsp1407I TGTACA 1 cut(s) 1338
Bsp143I GATC 8 cut(s) 189, 261, 396, 410, 1092, 1890, 2031, 2040
Bsp19I CCATGG 1 cut(s) 1584
BspACI CCGC 5 cut(s) 26, 90, 267, 357, 1538
BspANI GGCC 5 cut(s) 492, 1583, 1632, 1771, 1908
BspCNI CTCAG 3 cut(s) 592, 610, 1741
BspFNI CGCG 1 cut(s) 1434
BspHI TCATGA 1 cut(s) 1107
BspLI GGNNCC 4 cut(s) 24, 52, 1757, 2003
BspMAI CTGCAG 1 cut(s) 853
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 2 cut(s) 404, 1885
BspQI GCTCTTC 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 50
BspTNI GGTCTC 2 cut(s) 341, 1597
BsrDI GCAATG 2 cut(s) 981, 1514
BsrFI RCCGGY 4 cut(s) 47, 109, 239, 902
BsrGI TGTACA 1 cut(s) 1338
BsrI ACTGG 4 cut(s) 793, 1355, 1562, 2118
BssAI RCCGGY 4 cut(s) 47, 109, 239, 902
BssECI CCNNGG 3 cut(s) 342, 1584, 1760
BssMI GATC 8 cut(s) 189, 261, 396, 410, 1092, 1890, 2031, 2040
BssNAI GTATAC 2 cut(s) 890, 1942
BssNI GRCGYC 2 cut(s) 51, 1039
BssT1I CCWWGG 2 cut(s) 1584, 1760
Bst1107I GTATAC 2 cut(s) 890, 1942
Bst2UI CCWGG 3 cut(s) 344, 2009, 2071
Bst4CI ACNGT 2 cut(s) 1075, 1676
Bst6I CTCTTC 3 cut(s) 446, 674, 2089
BstACI GRCGYC 2 cut(s) 51, 1039
BstAPI GCANNNNNTGC 3 cut(s) 626, 1547, 2116
BstAUI TGTACA 1 cut(s) 1338
BstC8I GCNNGC 8 cut(s) 49, 359, 363, 618, 804, 1422, 1634, 1769
BstDEI CTNAG 4 cut(s) 579, 597, 1728, 1995
BstDSI CCRYGG 1 cut(s) 1584
BstF5I GGATG 3 cut(s) 225, 1181, 1834
BstFNI CGCG 1 cut(s) 1434
BstH2I RGCGCY 2 cut(s) 54, 375
BstHHI GCGC 3 cut(s) 53, 374, 704
BstKTI GATC 8 cut(s) 192, 264, 399, 413, 1095, 1893, 2034, 2043
BstMAI GTCTC 5 cut(s) 146, 341, 1021, 1597, 1721
BstMBI GATC 8 cut(s) 189, 261, 396, 410, 1092, 1890, 2031, 2040
BstNI CCWGG 3 cut(s) 344, 2009, 2071
BstSCI CCNGG 3 cut(s) 342, 2007, 2069
BstSFI CTRYAG 2 cut(s) 849, 2123
BstSLI GKGCMC 1 cut(s) 695
BstUI CGCG 1 cut(s) 1434
BstV1I GCAGC 8 cut(s) 15, 28, 31, 716, 1534, 1553, 1556, 1632
BstV2I GAAGAC 3 cut(s) 105, 504, 1670
BstX2I RGATCY 2 cut(s) 261, 396
BstXI CCANNNNNNTGG 1 cut(s) 1916
BstYI RGATCY 2 cut(s) 261, 396
BstZ17I GTATAC 2 cut(s) 890, 1942
BsuI GTATCC 1 cut(s) 1232
BsuRI GGCC 5 cut(s) 492, 1583, 1632, 1771, 1908
BtgI CCRYGG 1 cut(s) 1584
BtsCI GGATG 3 cut(s) 225, 1181, 1834
BtsI GCAGTG 1 cut(s) 1708
BtsIMutI CAGTG 4 cut(s) 192, 303, 1569, 1708
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 8 cut(s) 49, 359, 363, 618, 804, 1422, 1634, 1769
CaiI CAGNNNCTG 3 cut(s) 44, 1547, 1550
CciI TCATGA 1 cut(s) 1107
CfoI GCGC 3 cut(s) 53, 374, 704
Cfr10I RCCGGY 4 cut(s) 47, 109, 239, 902
CseI GACGC 1 cut(s) 1423
Csp6I GTAC 3 cut(s) 320, 877, 1339
CspAI ACCGGT 1 cut(s) 109
CviQI GTAC 3 cut(s) 320, 877, 1339
DdeI CTNAG 4 cut(s) 579, 597, 1728, 1995
DinI GGCGCC 1 cut(s) 52
DpnI GATC 8 cut(s) 191, 263, 398, 412, 1094, 1892, 2033, 2042
DpnII GATC 8 cut(s) 189, 261, 396, 410, 1092, 1890, 2031, 2040
DraI TTTAAA 1 cut(s) 480
Eam1104I CTCTTC 3 cut(s) 446, 674, 2089
EarI CTCTTC 3 cut(s) 446, 674, 2089
EciI GGCGGA 1 cut(s) 256
Ecl136II GAGCTC 1 cut(s) 1706
Eco130I CCWWGG 2 cut(s) 1584, 1760
Eco24I GRGCYC 2 cut(s) 669, 1708
Eco31I GGTCTC 2 cut(s) 341, 1597
Eco53kI GAGCTC 1 cut(s) 1706
Eco57I CTGAAG 1 cut(s) 1956
Eco88I CYCGRG 1 cut(s) 1028
EcoICRI GAGCTC 1 cut(s) 1706
EcoRI GAATTC 2 cut(s) 1098, 1227
EcoRII CCWGG 3 cut(s) 342, 2007, 2069
EcoT14I CCWWGG 2 cut(s) 1584, 1760
EcoT38I GRGCYC 2 cut(s) 669, 1708
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 1584, 1760
Esp3I CGTCTC 1 cut(s) 146
FaqI GGGAC 3 cut(s) 226, 1026, 1290
FauI CCCGC 2 cut(s) 83, 350
FauNDI CATATG 1 cut(s) 387
FbaI TGATCA 2 cut(s) 189, 2031
FblI GTMKAC 2 cut(s) 889, 1941
FokI GGATG 3 cut(s) 212, 1188, 1821
FriOI GRGCYC 2 cut(s) 669, 1708
FspBI CTAG 6 cut(s) 488, 515, 662, 1128, 1596, 1785
GlaI GCGC 3 cut(s) 52, 373, 703
GsaI CCCAGC 2 cut(s) 578, 2106
GsuI CTGGAG 3 cut(s) 365, 1545, 1991
HaeII RGCGCY 2 cut(s) 54, 375
HaeIII GGCC 5 cut(s) 492, 1583, 1632, 1771, 1908
HapII CCGG 6 cut(s) 48, 110, 240, 519, 903, 1626
HgaI GACGC 1 cut(s) 1423
HhaI GCGC 3 cut(s) 53, 374, 704
Hin1I GRCGYC 2 cut(s) 51, 1039
Hin6I GCGC 3 cut(s) 51, 372, 702
HinP1I GCGC 3 cut(s) 51, 372, 702
HincII GTYRAC 1 cut(s) 1744
HindII GTYRAC 1 cut(s) 1744
HinfI GANTC 5 cut(s) 1269, 1495, 1658, 1789, 1927
HpaII CCGG 6 cut(s) 48, 110, 240, 519, 903, 1626
HphI GGTGA 2 cut(s) 124, 892
Hpy166II GTNNAC 4 cut(s) 6, 890, 1744, 1942
Hpy188I TCNGA 8 cut(s) 251, 582, 600, 1097, 1695, 1890, 1936, 2016
Hpy188III TCNNGA 6 cut(s) 7, 259, 274, 1090, 1108, 2029
Hpy8I GTNNAC 4 cut(s) 6, 890, 1744, 1942
Hpy99I CGWCG 1 cut(s) 1686
HpyAV CCTTC 3 cut(s) 80, 341, 1253
HpyCH4III ACNGT 2 cut(s) 1075, 1676
HpyCH4IV ACGT 1 cut(s) 1039
HpyF3I CTNAG 4 cut(s) 579, 597, 1728, 1995
HpySE526I ACGT 1 cut(s) 1039
Hsp92I GRCGYC 2 cut(s) 51, 1039
HspAI GCGC 3 cut(s) 51, 372, 702
KasI GGCGCC 1 cut(s) 50
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Ksp22I TGATCA 2 cut(s) 189, 2031
Kzo9I GATC 8 cut(s) 189, 261, 396, 410, 1092, 1890, 2031, 2040
LguI GCTCTTC 1 cut(s) 674
LmnI GCTCC 9 cut(s) 22, 117, 1417, 1475, 1564, 1569, 1628, 1764, 2107
Lsp1109I GCAGC 8 cut(s) 15, 28, 31, 716, 1534, 1553, 1556, 1632
LweI GCATC 2 cut(s) 115, 1840
MaeI CTAG 6 cut(s) 488, 515, 662, 1128, 1596, 1785
MaeII ACGT 1 cut(s) 1039
MaeIII GTNAC 3 cut(s) 430, 1069, 1211
MalI GATC 8 cut(s) 191, 263, 398, 412, 1094, 1892, 2033, 2042
MboI GATC 8 cut(s) 189, 261, 396, 410, 1092, 1890, 2031, 2040
MboII GAAGA 7 cut(s) 110, 433, 454, 509, 661, 1670, 2076
MfeI CAATTG 2 cut(s) 741, 1164
MflI RGATCY 2 cut(s) 261, 396
MhlI GDGCHC 3 cut(s) 669, 695, 1708
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 1 cut(s) 1278
MmeI TCCRAC 2 cut(s) 764, 967
MroNI GCCGGC 1 cut(s) 47
MseI TTAA 5 cut(s) 324, 479, 717, 1121, 1368
MslI CAYNNNNRTG 3 cut(s) 203, 656, 1839
MspA1I CMGCKG 5 cut(s) 28, 41, 707, 1547, 2122
MspI CCGG 6 cut(s) 48, 110, 240, 519, 903, 1626
MspR9I CCNGG 3 cut(s) 344, 2009, 2071
MunI CAATTG 2 cut(s) 741, 1164
Mva1269I GAATGC 2 cut(s) 663, 2045
MvaI CCWGG 3 cut(s) 344, 2009, 2071
MvnI CGCG 1 cut(s) 1434
NaeI GCCGGC 1 cut(s) 49
NarI GGCGCC 1 cut(s) 51
NcoI CCATGG 1 cut(s) 1584
NdeI CATATG 1 cut(s) 387
NdeII GATC 8 cut(s) 189, 261, 396, 410, 1092, 1890, 2031, 2040
NgoMIV GCCGGC 1 cut(s) 47
NlaIV GGNNCC 4 cut(s) 24, 52, 1757, 2003
NmuCI GTSAC 1 cut(s) 1069
PagI TCATGA 1 cut(s) 1107
PciSI GCTCTTC 1 cut(s) 674
PcsI WCGNNNNNNNCGW 1 cut(s) 1699
PctI GAATGC 2 cut(s) 663, 2045
PdiI GCCGGC 1 cut(s) 49
PfeI GAWTC 4 cut(s) 1495, 1658, 1789, 1927
PinAI ACCGGT 1 cut(s) 109
PleI GAGTC 1 cut(s) 1277
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 1277
PshBI ATTAAT 1 cut(s) 1368
Psp124BI GAGCTC 1 cut(s) 1708
Psp6I CCWGG 3 cut(s) 342, 2007, 2069
PspFI CCCAGC 2 cut(s) 574, 2102
PspGI CCWGG 3 cut(s) 342, 2007, 2069
PspN4I GGNNCC 4 cut(s) 24, 52, 1757, 2003
PstI CTGCAG 1 cut(s) 853
PstNI CAGNNNCTG 3 cut(s) 44, 1547, 1550
PsuI RGATCY 2 cut(s) 261, 396
PvuII CAGCTG 4 cut(s) 41, 707, 1547, 2122
RsaI GTAC 3 cut(s) 321, 878, 1340
RsaNI GTAC 3 cut(s) 320, 877, 1339
RseI CAYNNNNRTG 3 cut(s) 203, 656, 1839
SacI GAGCTC 1 cut(s) 1708
SapI GCTCTTC 1 cut(s) 674
SaqAI TTAA 5 cut(s) 324, 479, 717, 1121, 1368
Sau3AI GATC 8 cut(s) 189, 261, 396, 410, 1092, 1890, 2031, 2040
SchI GAGTC 1 cut(s) 1278
ScrFI CCNGG 3 cut(s) 344, 2009, 2071
SduI GDGCHC 3 cut(s) 669, 695, 1708
SfaNI GCATC 2 cut(s) 115, 1840
SfcI CTRYAG 2 cut(s) 849, 2123
SfoI GGCGCC 1 cut(s) 52
SgrAI CRCCGGYG 1 cut(s) 109
SmiMI CAYNNNNRTG 3 cut(s) 203, 656, 1839
SmlI CTYRAG 2 cut(s) 747, 866
SmoI CTYRAG 2 cut(s) 747, 866
SsiI CCGC 5 cut(s) 26, 90, 267, 357, 1538
SspDI GGCGCC 1 cut(s) 50
SspI AATATT 2 cut(s) 552, 997
SspMI CTAG 6 cut(s) 488, 515, 662, 1128, 1596, 1785
SstI GAGCTC 1 cut(s) 1708
StyD4I CCNGG 3 cut(s) 342, 2007, 2069
StyI CCWWGG 2 cut(s) 1584, 1760
TaaI ACNGT 2 cut(s) 1075, 1676
TaiI ACGT 1 cut(s) 1042
TaqI TCGA 2 cut(s) 275, 1702
TaqII GACCGA 1 cut(s) 1674
TatI WGTACW 1 cut(s) 1338
TauI GCSGC 2 cut(s) 28, 1541
TfiI GAWTC 4 cut(s) 1495, 1658, 1789, 1927
Tru1I TTAA 5 cut(s) 324, 479, 717, 1121, 1368
Tru9I TTAA 5 cut(s) 324, 479, 717, 1121, 1368
TscAI CASTG 4 cut(s) 192, 310, 1569, 1715
TseFI GTSAC 1 cut(s) 1069
TseI GCWGC 9 cut(s) 28, 41, 44, 704, 1541, 1544, 1547, 1620, 2119
Tsp45I GTSAC 1 cut(s) 1069
TspDTI ATGAA 9 cut(s) 99, 374, 661, 786, 968, 1038, 1096, 1811, 2076
TspRI CASTG 4 cut(s) 192, 310, 1569, 1715
VspI ATTAAT 1 cut(s) 1368
XapI RAATTY 5 cut(s) 524, 824, 1098, 1227, 1898
XcmI CCANNNNNNNNNTGG 1 cut(s) 1445
XmiI GTMKAC 2 cut(s) 889, 1941
XspI CTAG 6 cut(s) 488, 515, 662, 1128, 1596, 1785
ZraI GACGTC 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.