Rmu_sc0005268.1_g000004

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005268.1
OG0000970
Physical Location & Seq
Forward (+)
8008 .. 10234
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005268.1_g000004.1.cds

Sequence Viewer

Length: 1503 bp
atggaggagccattactcgacgcagctgctgcaaattccgagttcaaccacaaggctttgtggggtgatggcaaagaagactacaagccagtgaggagctttcctgagtttcggtctatgttttggatagagacagtgaagctttggaaactagcaggtccttcaatgatcggattattggccatgtatgggacaaatgccgtcatcagcgtgtttgctggtcatattggaaccattgagctctccgcgatctccatttctctctctgtcatttccaccttctcttatggattcttgctcggcatggggagtgctttggagaccttggctggacaggcgtttggtgcaggacagattcatatgcttgggatatacatgcagcgctcatgtatagtcctacttgtaacttgcttcctcctgttgccattttacatctttgccactcccattttaaagttattaggccaagaagatgacatagccgatctcgctggagagttcacactgcaaataattcctcagttgttctcacttgccattaactttcctgctcagaagttccttcaggctcagagaaagctcaaagtcatgacgtggatttcggtggtggcgttgctcatacacgttgggctacttttcttcttcatttttgggttagattggggtaccttaggtgcagctgtggcatataatattaccagatggggcattgcaattgctcaagttgtatatattatggtctggtgcaatgagggttggactgggttttcatggttggcattcaaggatatgtggtcctttgttaggctttcaattgcctctgctgtcatgctttgccttgagatatggtacttcatgagtatgatgcttcttactggcaacctaaacgatgcagttctagcagttagttccctttctatttgcctcaatattaacgggtgggaaataatggtgtttgttggaataaatattgctataagtgttcgagtatcgaatgagcttggcttaggacgcccaagagcggccaagtatgcagtctatgtgacagtatttcagtctttcctaatagggattcttttcatgattctgatattgttattcaaagatagcttctctgcactttttactactgacaaggagctgcaacaagcggttgcacacctcgcataccttctcggcgcaactatgttgctcaacagtgtccagccagtaatatcaggtgttgcggttggcggtggatggcaggcaaccgtggcatatataaacttgggttgttactacatttttggaattcctctcggatacttgcttagtcacgtcgcagatttcggagtgatgggactttgggggggtatgatatgtggaactgctcttcagacattacttcttttatttgtgctttacaaaaccaattggaacaaagaggtggagcaagcatcaacacgcgtgcagaagtggggtttagataacaaaacagagactacagctgaaagtgctatatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

500

Amino Acids

55.11

Weight (kDa)

5.73

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 146
Acc65I GGTACC 1 cut(s) 665
AccB1I GGYRCC 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 1022
AccII CGCG 2 cut(s) 248, 1446
AciI CCGC 5 cut(s) 246, 1022, 1151, 1226, 1233
AcoI YGGCCR 2 cut(s) 180, 1023
AcsI RAATTY 2 cut(s) 34, 1290
AcuI CTGAAG 2 cut(s) 548, 1358
AcyI GRCGYC 1 cut(s) 1012
AfaI GTAC 2 cut(s) 667, 851
AfeI AGCGCT 1 cut(s) 383
AfiI CCNNNNNNNGG 3 cut(s) 189, 804, 1021
AflIII ACRYGT 2 cut(s) 622, 1444
AgsI TTSAA 5 cut(s) 46, 165, 784, 813, 1102
AjiI CACGTC 2 cut(s) 594, 1318
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 3 cut(s) 125, 314, 1472
AlwNI CAGNNNCTG 1 cut(s) 29
Aor51HI AGCGCT 1 cut(s) 383
AoxI GGCC 3 cut(s) 180, 463, 1023
ApeKI GCWGC 6 cut(s) 23, 26, 29, 379, 677, 1141
ApoI RAATTY 2 cut(s) 34, 1290
ArsI GACNNNNNNTTYG 2 cut(s) 583, 615
Asp718I GGTACC 1 cut(s) 665
AspLEI GCGC 2 cut(s) 384, 1181
AspS9I GGNCC 2 cut(s) 158, 795
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 2 cut(s) 158, 795
AxyI CCTNAGG 1 cut(s) 670
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 6 cut(s) 13, 16, 35, 391, 689, 1128
BccI CCATC 4 cut(s) 62, 696, 1233, 1330
BceAI ACGGC 1 cut(s) 185
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BciVI GTATCC 1 cut(s) 1295
BcoDI GTCTC 3 cut(s) 125, 314, 1472
BfaI CTAG 2 cut(s) 152, 899
BfmI CTRYAG 1 cut(s) 1482
BfoI RGCGCY 1 cut(s) 385
BfuAI ACCTGC 1 cut(s) 146
BfuI GTATCC 1 cut(s) 1295
BisI GCNGC 7 cut(s) 24, 27, 30, 380, 678, 1023, 1142
BlsI GCNGC 7 cut(s) 25, 28, 31, 381, 679, 1024, 1143
Bme18I GGWCC 2 cut(s) 158, 795
BmgBI CACGTC 2 cut(s) 594, 1318
BmgT120I GGNCC 2 cut(s) 158, 795
BmiI GGNNCC 3 cut(s) 9, 232, 667
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 3 cut(s) 855, 880, 1445
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 1006
BpuEI CTTGAG 2 cut(s) 705, 860
BsaHI GRCGYC 1 cut(s) 1012
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 324, 1251
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 804, 1021
Bse1I ACTGG 4 cut(s) 89, 766, 880, 1208
Bse21I CCTNAGG 1 cut(s) 670
Bse3DI GCAATG 2 cut(s) 708, 754
BseDI CCNNGG 2 cut(s) 324, 1251
BseGI GGATG 1 cut(s) 1244
BseLI CCNNNNNNNGG 3 cut(s) 189, 804, 1021
BseMI GCAATG 2 cut(s) 708, 754
BseMII CTCAG 4 cut(s) 96, 533, 566, 584
BseNI ACTGG 4 cut(s) 89, 766, 880, 1208
BseRI GAGGAG 2 cut(s) 20, 109
BseXI GCAGC 6 cut(s) 13, 16, 35, 391, 689, 1128
BsgI GTGCAG 4 cut(s) 366, 696, 1101, 1469
Bsh1236I CGCG 2 cut(s) 248, 1446
BshFI GGCC 3 cut(s) 182, 465, 1025
BshNI GGYRCC 1 cut(s) 665
BsiHKAI GWGCWC 1 cut(s) 243
BslFI GGGAC 2 cut(s) 205, 1353
BslI CCNNNNNNNGG 3 cut(s) 189, 804, 1021
BsmAI GTCTC 3 cut(s) 125, 314, 1472
BsmFI GGGAC 2 cut(s) 205, 1353
BsmI GAATGC 1 cut(s) 779
BsnI GGCC 3 cut(s) 182, 465, 1025
Bso31I GGTCTC 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 3 cut(s) 168, 249, 484
BspACI CCGC 5 cut(s) 246, 1022, 1151, 1226, 1233
BspANI GGCC 3 cut(s) 182, 465, 1025
BspCNI CTCAG 4 cut(s) 97, 532, 565, 583
BspFNI CGCG 2 cut(s) 248, 1446
BspHI TCATGA 3 cut(s) 588, 855, 1080
BspLI GGNNCC 3 cut(s) 9, 232, 667
BspMI ACCTGC 1 cut(s) 146
BspQI GCTCTTC 1 cut(s) 1377
BspT107I GGYRCC 1 cut(s) 665
BspTNI GGTCTC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 1022
BsrDI GCAATG 2 cut(s) 708, 754
BsrI ACTGG 4 cut(s) 89, 766, 880, 1208
BssECI CCNNGG 2 cut(s) 324, 1251
BssMI GATC 3 cut(s) 168, 249, 484
BssNI GRCGYC 1 cut(s) 1012
BssT1I CCWWGG 1 cut(s) 324
Bst4CI ACNGT 4 cut(s) 136, 1048, 1199, 1252
Bst6I CTCTTC 1 cut(s) 1377
BstACI GRCGYC 1 cut(s) 1012
BstAPI GCANNNNNTGC 1 cut(s) 29
BstC8I GCNNGC 3 cut(s) 1245, 1434, 1448
BstDEI CTNAG 7 cut(s) 105, 519, 552, 570, 670, 1006, 1310
BstDSI CCRYGG 1 cut(s) 1251
BstENI CCTNNNNNAGG 1 cut(s) 802
BstF5I GGATG 1 cut(s) 1244
BstFNI CGCG 2 cut(s) 248, 1446
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 2 cut(s) 384, 1181
BstKTI GATC 3 cut(s) 171, 252, 487
BstMAI GTCTC 3 cut(s) 125, 314, 1472
BstMBI GATC 3 cut(s) 168, 249, 484
BstNSI RCATGY 1 cut(s) 379
BstSFI CTRYAG 1 cut(s) 1482
BstUI CGCG 2 cut(s) 248, 1446
BstV1I GCAGC 6 cut(s) 13, 16, 35, 391, 689, 1128
BstV2I GAAGAC 1 cut(s) 84
Bsu36I CCTNAGG 1 cut(s) 670
BsuI GTATCC 1 cut(s) 1295
BsuRI GGCC 3 cut(s) 182, 465, 1025
BtgI CCRYGG 1 cut(s) 1251
BtrI CACGTC 2 cut(s) 594, 1318
BtsCI GGATG 1 cut(s) 1244
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 4 cut(s) 96, 141, 503, 1204
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 3 cut(s) 1245, 1434, 1448
CaiI CAGNNNCTG 1 cut(s) 29
CciI TCATGA 3 cut(s) 588, 855, 1080
CfoI GCGC 2 cut(s) 384, 1181
Cfr13I GGNCC 2 cut(s) 158, 795
CseI GACGC 2 cut(s) 29, 1020
Csp6I GTAC 2 cut(s) 666, 850
CviAII CATG 9 cut(s) 184, 304, 376, 387, 589, 771, 829, 856, 1081
CviQI GTAC 2 cut(s) 666, 850
DdeI CTNAG 7 cut(s) 105, 519, 552, 570, 670, 1006, 1310
DpnI GATC 3 cut(s) 170, 251, 486
DpnII GATC 3 cut(s) 168, 249, 484
DraI TTTAAA 1 cut(s) 453
EaeI YGGCCR 2 cut(s) 180, 1023
Eam1104I CTCTTC 1 cut(s) 1377
EarI CTCTTC 1 cut(s) 1377
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 2 cut(s) 158, 795
Eco47III AGCGCT 1 cut(s) 383
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 548, 1358
Eco81I CCTNAGG 1 cut(s) 670
EcoICRI GAGCTC 1 cut(s) 241
EcoNI CCTNNNNNAGG 1 cut(s) 802
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 1290
EcoT14I CCWWGG 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 9 cut(s) 187, 307, 379, 390, 592, 774, 832, 859, 1084
FaqI GGGAC 2 cut(s) 205, 1353
FatI CATG 9 cut(s) 183, 303, 375, 386, 588, 770, 828, 855, 1080
FauNDI CATATG 1 cut(s) 360
Fnu4HI GCNGC 7 cut(s) 24, 27, 30, 380, 678, 1023, 1142
FokI GGATG 1 cut(s) 1251
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 7 cut(s) 24, 27, 30, 380, 678, 1023, 1142
FspBI CTAG 2 cut(s) 152, 899
GlaI GCGC 2 cut(s) 383, 1180
GluI GCNGC 7 cut(s) 24, 27, 30, 380, 678, 1023, 1142
GsuI CTGGAG 1 cut(s) 513
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 3 cut(s) 182, 465, 1025
HgaI GACGC 2 cut(s) 29, 1020
HhaI GCGC 2 cut(s) 384, 1181
Hin1I GRCGYC 1 cut(s) 1012
Hin1II CATG 9 cut(s) 187, 307, 379, 390, 592, 774, 832, 859, 1084
Hin6I GCGC 2 cut(s) 382, 1179
HinP1I GCGC 2 cut(s) 382, 1179
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 4 cut(s) 291, 355, 1072, 1084
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 501
Hpy188I TCNGA 8 cut(s) 40, 173, 555, 573, 1089, 1301, 1331, 1377
Hpy188III TCNNGA 4 cut(s) 104, 589, 856, 1081
Hpy8I GTNNAC 1 cut(s) 501
Hpy99I CGWCG 2 cut(s) 23, 1322
HpyAV CCTTC 4 cut(s) 171, 289, 572, 1181
HpyCH4III ACNGT 4 cut(s) 136, 1048, 1199, 1252
HpyCH4IV ACGT 3 cut(s) 593, 624, 1317
HpyF3I CTNAG 7 cut(s) 105, 519, 552, 570, 670, 1006, 1310
HpySE526I ACGT 3 cut(s) 593, 624, 1317
Hsp92I GRCGYC 1 cut(s) 1012
Hsp92II CATG 9 cut(s) 187, 307, 379, 390, 592, 774, 832, 859, 1084
HspAI GCGC 2 cut(s) 382, 1179
KpnI GGTACC 1 cut(s) 669
Kzo9I GATC 3 cut(s) 168, 249, 484
LguI GCTCTTC 1 cut(s) 1377
LmnI GCTCC 4 cut(s) 7, 96, 1138, 1429
Lsp1109I GCAGC 6 cut(s) 13, 16, 35, 391, 689, 1128
LweI GCATC 3 cut(s) 855, 880, 1445
MaeI CTAG 2 cut(s) 152, 899
MaeII ACGT 3 cut(s) 593, 624, 1317
MaeIII GTNAC 4 cut(s) 403, 1042, 1274, 1313
MalI GATC 3 cut(s) 170, 251, 486
MbiI CCGCTC 1 cut(s) 1022
MboI GATC 3 cut(s) 168, 249, 484
MboII GAAGA 5 cut(s) 89, 482, 631, 634, 1364
MfeI CAATTG 3 cut(s) 714, 813, 1411
MhlI GDGCHC 1 cut(s) 243
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 6 cut(s) 34, 513, 714, 813, 1290, 1411
MluI ACGCGT 1 cut(s) 1444
MluNI TGGCCA 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 737, 940
MnlI CCTC 9 cut(s) 87, 425, 528, 745, 829, 935, 1172, 1305, 1417
Mox20I TGGCCA 1 cut(s) 182
MscI TGGCCA 1 cut(s) 182
MseI TTAA 3 cut(s) 452, 540, 933
MslI CAYNNNNRTG 2 cut(s) 209, 860
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 3 cut(s) 26, 680, 1487
MunI CAATTG 3 cut(s) 714, 813, 1411
Mva1269I GAATGC 1 cut(s) 779
MvnI CGCG 2 cut(s) 248, 1446
NdeI CATATG 1 cut(s) 360
NdeII GATC 3 cut(s) 168, 249, 484
NlaIII CATG 9 cut(s) 187, 307, 379, 390, 592, 774, 832, 859, 1084
NlaIV GGNNCC 3 cut(s) 9, 232, 667
NmeAIII GCCGAG 2 cut(s) 279, 1155
NmuCI GTSAC 2 cut(s) 1042, 1313
NspI RCATGY 1 cut(s) 379
PagI TCATGA 3 cut(s) 588, 855, 1080
PciSI GCTCTTC 1 cut(s) 1377
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PctI GAATGC 1 cut(s) 779
PfeI GAWTC 4 cut(s) 291, 355, 1072, 1084
PkrI GCNGC 7 cut(s) 25, 28, 31, 381, 679, 1024, 1143
PpuMI RGGWCCY 1 cut(s) 158
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 158
PspN4I GGNNCC 3 cut(s) 9, 232, 667
PspPI GGNCC 2 cut(s) 158, 795
PspPPI RGGWCCY 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 3 cut(s) 26, 680, 1487
RsaI GTAC 2 cut(s) 667, 851
RsaNI GTAC 2 cut(s) 666, 850
RseI CAYNNNNRTG 2 cut(s) 209, 860
SacI GAGCTC 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 1377
SaqAI TTAA 3 cut(s) 452, 540, 933
SatI GCNGC 7 cut(s) 24, 27, 30, 380, 678, 1023, 1142
Sau3AI GATC 3 cut(s) 168, 249, 484
Sau96I GGNCC 2 cut(s) 158, 795
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 3 cut(s) 855, 880, 1445
SfcI CTRYAG 1 cut(s) 1482
SinI GGWCC 2 cut(s) 158, 795
SmiMI CAYNNNNRTG 2 cut(s) 209, 860
SmlI CTYRAG 2 cut(s) 720, 839
SmoI CTYRAG 2 cut(s) 720, 839
Sse9I AATT 6 cut(s) 34, 513, 714, 813, 1290, 1411
SsiI CCGC 5 cut(s) 246, 1022, 1151, 1226, 1233
SspI AATATT 3 cut(s) 694, 931, 970
SspMI CTAG 2 cut(s) 152, 899
SstI GAGCTC 1 cut(s) 243
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 4 cut(s) 136, 1048, 1199, 1252
TaiI ACGT 3 cut(s) 596, 627, 1320
TaqI TCGA 3 cut(s) 18, 985, 992
TaqII GACCGA 1 cut(s) 102
TasI AATT 6 cut(s) 34, 513, 714, 813, 1290, 1411
TauI GCSGC 1 cut(s) 1025
TfiI GAWTC 4 cut(s) 291, 355, 1072, 1084
Tru1I TTAA 3 cut(s) 452, 540, 933
Tru9I TTAA 3 cut(s) 452, 540, 933
TscAI CASTG 4 cut(s) 96, 141, 510, 1204
TseFI GTSAC 2 cut(s) 1042, 1313
TseI GCWGC 6 cut(s) 23, 26, 29, 379, 677, 1141
Tsp45I GTSAC 2 cut(s) 1042, 1313
TspDTI ATGAA 5 cut(s) 347, 634, 759, 844, 1069
TspRI CASTG 4 cut(s) 96, 141, 510, 1204
VpaK11BI GGWCC 2 cut(s) 158, 795
XagI CCTNNNNNAGG 1 cut(s) 802
XapI RAATTY 2 cut(s) 34, 1290
XceI RCATGY 1 cut(s) 379
XspI CTAG 2 cut(s) 152, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.