pycom01g16260

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000970
Physical Location & Seq
Reverse (-)
15907579 .. 15918774
11196 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16260.3

Sequence Viewer

Length: 1509 bp
ATGGAGAAGCCGCTGCTCCACACAGCCGGTGACGGTGACGTTGAGTTGAGCAATAAGCCACTTCTTTATGAAGGCACCAATGAAGATTATGCACCGGTGAGGAGCTTTGATGCATTCCGGCGTATGTTTTGGATAGAGACAGTGAAGCTGTGGCAAATAGCCGGTTCGACTGTGATCACTATGATGTGTATGTATGGAAATACCGCAGTTGTTGTGCTCTTTGCTGGTCATCTCGGAACTATTGAGCTCTCTGCCATCTCCATTTCGCTCACCGTCATCTCCATATTCTCTTTTGGAGTCATGCTTGGGATGGGGAGTGCTCTGGAGACCCTATGTGGACAAGCATTTGGTGCTGGACAAGTTCATATGCTTGGAATCTACATGCAGCGCTCATGTATAATCCTATTTGTAACCAGCATCTTCATCTTGCCGACCTACATCTTTGCCACGCCGATTCTAAAGTGGTTGGGCCAAGAAGACGACATAGCTAATCTAGCCGGAAAATTCACACTCCAAATAATCCCCCATCTGTTCTCACTTGCTGTAATTTTCCCAACTCAGAAGTTCCTCCAGTCTCAGAGGAAGGTGAAGGTGCTGGCATGGATTGCTGTTTTGGGTTTGATCGTAGAGATAGGAATGCTAGGGCTCTTCATCTATGTGTTCGATTGGGGCACAACGGGGGCAGCTGTGTCATTTACCGTCACGAGATGGGGGATGGCAATTACTCAAGTTGTGTATGTTATGGGTTGGTGTAAGGAAGGTTGGACTGGATTTTCATGGCTGGCATTCAAGGAGATTTGGGCATTTGTAAGACTTTCCCTTGCCTCTGCTGTGATGCTCTGCCTGGAAATTTGGTACATGATGAGTATTATCATTCTCACAGGACATCTTTCTAATGCAGTTATAGCCGTTGGCTCTCTTTCTATCTGCATGAGCATCAATGGATGGGAAGGAATCTTGTTCGTTGGAATAAACGCCGCAATAAGTGTTCGTGTCTCTAATGAGCTTGGATTAGGACGTCCCAGAGCAGCCAAATACTCTGTCTATGTTACAATATTTCAATCTCTTGTACTTGGGATTCTTTTCATGGTTCTTATTTTAATAACTAGAGACGATTTCGCCGTACTTTATACAAGTGACAAGGAGCTGCAACAAGCAGTCACTAAACTAGCTTACCTTCTTGGTATAACTATGTTGCTTAACAGTGTCCAACCAGTAATATCAGGTGTTGCAGTTGGAGGTGGATGGCAGGCAATGGTAGCTTATATAAACTTGGGTTGTTACTACATTTTTGGACTTCCTCTTGGATACCTTCTTTGCCATGTTGCAAATTTGGGAGTCATGGGCCTTTGGGGAGGCATGATATGTGGAACCGCTCTCCAAACCATACTTCTGCTAATTATACTTTACAAAACCAACTGGAACACAGAGGTGGAGCAAGCAACAAATCGCGTTCGGAAGTGGGGTGGGCAAGACATCAAAACTGAGAATGTAGCTGAAATTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

503

Amino Acids

55.26

Weight (kDa)

6.6

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1021
AccB1I GGYRCC 1 cut(s) 74
AccBSI CCGCTC 1 cut(s) 1376
AccII CGCG 1 cut(s) 1452
AciI CCGC 4 cut(s) 11, 204, 978, 1374
AcsI RAATTY 3 cut(s) 503, 849, 1330
AcyI GRCGYC 1 cut(s) 1018
AfaI GTAC 3 cut(s) 857, 1071, 1125
AfeI AGCGCT 1 cut(s) 389
AgeI ACCGGT 1 cut(s) 94
AgsI TTSAA 2 cut(s) 790, 1061
AjnI CCWGG 1 cut(s) 843
AjuI GAANNNNNNNTTGG 2 cut(s) 1374, 1406
AleI CACNNNNGTG 1 cut(s) 1430
Alw21I GWGCWC 3 cut(s) 219, 249, 322
Alw26I GTCTC 5 cut(s) 131, 320, 579, 1000, 1104
Aor51HI AGCGCT 1 cut(s) 389
AoxI GGCC 2 cut(s) 469, 1345
ApeKI GCWGC 5 cut(s) 13, 385, 683, 1028, 1147
ApoI RAATTY 3 cut(s) 503, 849, 1330
ArsI GACNNNNNNTTYG 2 cut(s) 1026, 1058
AsiGI ACCGGT 1 cut(s) 94
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 2 cut(s) 469, 1345
AsuHPI GGTGA 5 cut(s) 41, 47, 109, 262, 598
BaeGI GKGCMC 1 cut(s) 674
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 2 cut(s) 249, 648
BauI CACGAG 1 cut(s) 703
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 3 cut(s) 219, 249, 322
BbvI GCAGC 4 cut(s) 397, 695, 1040, 1134
BccI CCATC 7 cut(s) 263, 304, 534, 702, 709, 939, 1239
BceAI ACGGC 2 cut(s) 893, 1106
BciT130I CCWGG 1 cut(s) 845
BciVI GTATCC 1 cut(s) 1301
BclI TGATCA 1 cut(s) 174
BcoDI GTCTC 5 cut(s) 131, 320, 579, 1000, 1104
BfaI CTAG 4 cut(s) 494, 641, 1107, 1169
BfoI RGCGCY 1 cut(s) 391
BfuI GTATCC 1 cut(s) 1301
BisI GCNGC 7 cut(s) 11, 14, 386, 684, 978, 1029, 1148
BlsI GCNGC 7 cut(s) 12, 15, 387, 685, 979, 1030, 1149
Bme1390I CCNGG 1 cut(s) 845
BmgT120I GGNCC 2 cut(s) 469, 1345
BmiI GGNNCC 2 cut(s) 76, 1372
BmrFI CCNGG 1 cut(s) 845
BmsI GCATC 4 cut(s) 100, 426, 825, 945
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 2 cut(s) 344, 554
BpuEI CTTGAG 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 1018
BsaI GGTCTC 1 cut(s) 320
BsaWI WCCGGW 1 cut(s) 94
Bse118I RCCGGY 3 cut(s) 26, 94, 161
Bse1I ACTGG 4 cut(s) 571, 772, 1214, 1424
Bse3DI GCAATG 1 cut(s) 1260
BseBI CCWGG 1 cut(s) 845
BseGI GGATG 4 cut(s) 315, 720, 950, 1250
BseMI GCAATG 1 cut(s) 1260
BseMII CTCAG 3 cut(s) 572, 590, 1476
BseNI ACTGG 4 cut(s) 571, 772, 1214, 1424
BseRI GAGGAG 1 cut(s) 115
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 4 cut(s) 397, 695, 1040, 1134
Bsh1236I CGCG 1 cut(s) 1452
BshFI GGCC 2 cut(s) 471, 1347
BshNI GGYRCC 1 cut(s) 74
BshTI ACCGGT 1 cut(s) 94
BsiHKAI GWGCWC 3 cut(s) 219, 249, 322
BsiSI CCGG 5 cut(s) 27, 95, 118, 162, 498
BslFI GGGAC 1 cut(s) 1005
BsmAI GTCTC 5 cut(s) 131, 320, 579, 1000, 1104
BsmBI CGTCTC 1 cut(s) 1104
BsmFI GGGAC 1 cut(s) 1005
BsmI GAATGC 3 cut(s) 113, 642, 785
BsnI GGCC 2 cut(s) 471, 1347
Bso31I GGTCTC 1 cut(s) 320
Bsp1286I GDGCHC 5 cut(s) 219, 249, 322, 648, 674
Bsp143I GATC 2 cut(s) 174, 621
BspACI CCGC 4 cut(s) 11, 204, 978, 1374
BspANI GGCC 2 cut(s) 471, 1347
BspCNI CTCAG 3 cut(s) 571, 589, 1477
BspFNI CGCG 1 cut(s) 1452
BspLI GGNNCC 2 cut(s) 76, 1372
BspQI GCTCTTC 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 320
BsrBI CCGCTC 1 cut(s) 1376
BsrDI GCAATG 1 cut(s) 1260
BsrFI RCCGGY 3 cut(s) 26, 94, 161
BsrI ACTGG 4 cut(s) 571, 772, 1214, 1424
BssAI RCCGGY 3 cut(s) 26, 94, 161
BssMI GATC 2 cut(s) 174, 621
BssNI GRCGYC 1 cut(s) 1018
BssSI CACGAG 1 cut(s) 703
Bst2BI CACGAG 1 cut(s) 703
Bst2UI CCWGG 1 cut(s) 845
Bst4CI ACNGT 6 cut(s) 35, 142, 172, 274, 700, 1205
Bst6I CTCTTC 1 cut(s) 653
BstACI GRCGYC 1 cut(s) 1018
BstAPI GCANNNNNTGC 2 cut(s) 350, 605
BstC8I GCNNGC 4 cut(s) 597, 783, 1251, 1440
BstDEI CTNAG 3 cut(s) 558, 576, 1485
BstF5I GGATG 4 cut(s) 315, 720, 950, 1250
BstFNI CGCG 1 cut(s) 1452
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 2 cut(s) 177, 624
BstMAI GTCTC 5 cut(s) 131, 320, 579, 1000, 1104
BstMBI GATC 2 cut(s) 174, 621
BstMWI GCNNNNNNNGC 5 cut(s) 350, 494, 605, 905, 1259
BstNI CCWGG 1 cut(s) 845
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 843
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 1 cut(s) 1452
BstV1I GCAGC 4 cut(s) 397, 695, 1040, 1134
BstV2I GAAGAC 1 cut(s) 483
BsuI GTATCC 1 cut(s) 1301
BsuRI GGCC 2 cut(s) 471, 1347
BtsCI GGATG 4 cut(s) 315, 720, 950, 1250
BtsIMutI CAGTG 2 cut(s) 147, 1210
Cac8I GCNNGC 4 cut(s) 597, 783, 1251, 1440
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 3 cut(s) 26, 94, 161
Cfr13I GGNCC 2 cut(s) 469, 1345
Csp6I GTAC 3 cut(s) 856, 1070, 1124
CspAI ACCGGT 1 cut(s) 94
CviQI GTAC 3 cut(s) 856, 1070, 1124
DdeI CTNAG 3 cut(s) 558, 576, 1485
DpnI GATC 2 cut(s) 176, 623
DpnII GATC 2 cut(s) 174, 621
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
Ecl136II GAGCTC 1 cut(s) 247
Eco24I GRGCYC 2 cut(s) 249, 648
Eco31I GGTCTC 1 cut(s) 320
Eco47III AGCGCT 1 cut(s) 389
Eco53kI GAGCTC 1 cut(s) 247
EcoICRI GAGCTC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 843
EcoT22I ATGCAT 1 cut(s) 115
EcoT38I GRGCYC 2 cut(s) 249, 648
Esp3I CGTCTC 1 cut(s) 1104
FaqI GGGAC 1 cut(s) 1005
FauNDI CATATG 1 cut(s) 366
FbaI TGATCA 1 cut(s) 174
Fnu4HI GCNGC 7 cut(s) 11, 14, 386, 684, 978, 1029, 1148
FokI GGATG 4 cut(s) 322, 727, 957, 1257
FriOI GRGCYC 2 cut(s) 249, 648
Fsp4HI GCNGC 7 cut(s) 11, 14, 386, 684, 978, 1029, 1148
FspBI CTAG 4 cut(s) 494, 641, 1107, 1169
GlaI GCGC 1 cut(s) 389
GluI GCNGC 7 cut(s) 11, 14, 386, 684, 978, 1029, 1148
GsuI CTGGAG 2 cut(s) 344, 554
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 2 cut(s) 471, 1347
HapII CCGG 5 cut(s) 27, 95, 118, 162, 498
HhaI GCGC 1 cut(s) 390
Hin1I GRCGYC 1 cut(s) 1018
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HinfI GANTC 6 cut(s) 297, 375, 454, 954, 1078, 1338
HpaII CCGG 5 cut(s) 27, 95, 118, 162, 498
HphI GGTGA 5 cut(s) 41, 47, 109, 262, 598
Hpy166II GTNNAC 1 cut(s) 338
Hpy188I TCNGA 4 cut(s) 236, 561, 579, 1458
Hpy188III TCNNGA 2 cut(s) 323, 703
Hpy8I GTNNAC 1 cut(s) 338
HpyAV CCTTC 7 cut(s) 65, 577, 583, 752, 944, 1187, 1322
HpyCH4III ACNGT 6 cut(s) 35, 142, 172, 274, 700, 1205
HpyCH4IV ACGT 2 cut(s) 39, 1018
HpyCH4V TGCA 8 cut(s) 92, 113, 385, 899, 930, 1150, 1232, 1328
HpyF10VI GCNNNNNNNGC 5 cut(s) 350, 494, 605, 905, 1259
HpyF3I CTNAG 3 cut(s) 558, 576, 1485
HpySE526I ACGT 2 cut(s) 39, 1018
Hsp92I GRCGYC 1 cut(s) 1018
HspAI GCGC 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 2 cut(s) 174, 621
LguI GCTCTTC 1 cut(s) 653
LmnI GCTCC 4 cut(s) 21, 102, 1144, 1435
Lsp1109I GCAGC 4 cut(s) 397, 695, 1040, 1134
LweI GCATC 4 cut(s) 100, 426, 825, 945
MaeI CTAG 4 cut(s) 494, 641, 1107, 1169
MaeII ACGT 2 cut(s) 39, 1018
MaeIII GTNAC 8 cut(s) 29, 35, 409, 700, 1048, 1136, 1159, 1280
MalI GATC 2 cut(s) 176, 623
MbiI CCGCTC 1 cut(s) 1376
MboI GATC 2 cut(s) 174, 621
MboII GAAGA 4 cut(s) 95, 412, 488, 640
MhlI GDGCHC 5 cut(s) 219, 249, 322, 648, 674
MluCI AATT 7 cut(s) 503, 546, 720, 849, 1330, 1398, 1500
MlyI GAGTC 2 cut(s) 306, 1347
MmeI TCCRAC 4 cut(s) 743, 946, 1216, 1234
MnlI CCTC 8 cut(s) 93, 573, 578, 835, 1232, 1311, 1349, 1423
Mph1103I ATGCAT 1 cut(s) 115
MseI TTAA 2 cut(s) 1100, 1200
MslI CAYNNNNRTG 3 cut(s) 182, 656, 1430
MspA1I CMGCKG 2 cut(s) 13, 686
MspI CCGG 5 cut(s) 27, 95, 118, 162, 498
MspR9I CCNGG 1 cut(s) 845
Mva1269I GAATGC 3 cut(s) 113, 642, 785
MvaI CCWGG 1 cut(s) 845
MvnI CGCG 1 cut(s) 1452
MwoI GCNNNNNNNGC 5 cut(s) 350, 494, 605, 905, 1259
NdeI CATATG 1 cut(s) 366
NdeII GATC 2 cut(s) 174, 621
NlaIV GGNNCC 2 cut(s) 76, 1372
NmuCI GTSAC 5 cut(s) 29, 35, 700, 1136, 1159
NsiI ATGCAT 1 cut(s) 115
NspI RCATGY 1 cut(s) 385
OliI CACNNNNGTG 1 cut(s) 1430
PciSI GCTCTTC 1 cut(s) 653
PcsI WCGNNNNNNNCGW 1 cut(s) 1119
PctI GAATGC 3 cut(s) 113, 642, 785
PfeI GAWTC 4 cut(s) 375, 454, 954, 1078
PinAI ACCGGT 1 cut(s) 94
PkrI GCNGC 7 cut(s) 12, 15, 387, 685, 979, 1030, 1149
PleI GAGTC 2 cut(s) 305, 1346
PpsI GAGTC 2 cut(s) 305, 1346
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 843
PspGI CCWGG 1 cut(s) 843
PspN4I GGNNCC 2 cut(s) 76, 1372
PspPI GGNCC 2 cut(s) 469, 1345
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 3 cut(s) 857, 1071, 1125
RsaNI GTAC 3 cut(s) 856, 1070, 1124
RseI CAYNNNNRTG 3 cut(s) 182, 656, 1430
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 653
SaqAI TTAA 2 cut(s) 1100, 1200
SatI GCNGC 7 cut(s) 11, 14, 386, 684, 978, 1029, 1148
Sau3AI GATC 2 cut(s) 174, 621
Sau96I GGNCC 2 cut(s) 469, 1345
SchI GAGTC 2 cut(s) 306, 1347
ScrFI CCNGG 1 cut(s) 845
SduI GDGCHC 5 cut(s) 219, 249, 322, 648, 674
SfaNI GCATC 4 cut(s) 100, 426, 825, 945
SgrAI CRCCGGYG 1 cut(s) 94
SmiMI CAYNNNNRTG 3 cut(s) 182, 656, 1430
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
Sse9I AATT 7 cut(s) 503, 546, 720, 849, 1330, 1398, 1500
SsiI CCGC 4 cut(s) 11, 204, 978, 1374
SspI AATATT 1 cut(s) 1056
SspMI CTAG 4 cut(s) 494, 641, 1107, 1169
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 843
TaaI ACNGT 6 cut(s) 35, 142, 172, 274, 700, 1205
TaiI ACGT 2 cut(s) 42, 1021
TaqI TCGA 2 cut(s) 167, 663
TasI AATT 7 cut(s) 503, 546, 720, 849, 1330, 1398, 1500
TatI WGTACW 1 cut(s) 1069
TauI GCSGC 2 cut(s) 13, 980
TfiI GAWTC 4 cut(s) 375, 454, 954, 1078
Tru1I TTAA 2 cut(s) 1100, 1200
Tru9I TTAA 2 cut(s) 1100, 1200
TscAI CASTG 2 cut(s) 147, 1210
TseFI GTSAC 5 cut(s) 29, 35, 700, 1136, 1159
TseI GCWGC 5 cut(s) 13, 385, 683, 1028, 1147
Tsp45I GTSAC 5 cut(s) 29, 35, 700, 1136, 1159
TspDTI ATGAA 7 cut(s) 84, 96, 353, 412, 640, 765, 1075
TspRI CASTG 2 cut(s) 147, 1210
XapI RAATTY 3 cut(s) 503, 849, 1330
XceI RCATGY 1 cut(s) 385
XspI CTAG 4 cut(s) 494, 641, 1107, 1169
ZraI GACGTC 1 cut(s) 1019
Zsp2I ATGCAT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.