Rh1AG425200

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000970
Physical Location & Seq
Forward (+)
65420552 .. 65424015
3464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG425200.1

Sequence Viewer

Length: 1215 bp
ATGGAGGCGCCGCTGCTGACCAACGGAGCTGTCACGGACTACGAGCCGGTGACAAGCTTTAAGCAGGCTCGGGAGGTGACGTGGAAAGAAACAGTGAAGCTATGGAGGATTGGTGGGCCTATAGCCTTCACCATAATCTGCAACTTCGGCACCAACTCCGTCTCCACCATGTTCGTCGGCCACATCGGAAACATTGAGCTCTCCGCCGTCTCTATCTCTCTCTCCGTCATCTGTACCTTCTCTTTCGGCTTCATGCTTGGTATGGGAAGTGCACTAGAGACATTATGTGGTCAAGCATTTGGAGCTGGACGAGTTAACATGCTTGGTATCTACATGCAAAGGTCATGGATCATTCTATGGATAACCTGCCTTTTCCTTACTCCACTCTATTGCTTCTCTGCCCCAATCTTAAAGCTTCTTGGCCAAGATGACGATGTAGCGGACCTGGCGGGAACATTCACCGTGTATGTGATCCCTCAGCTATTCTCACTTGCCATCAATTTCCCTTGCCAAAAGTTCCTGCAGGCACAAAGCAAGGTCATAGTTCTTGCTTGGATCGGATTCATAGCCCTAATGGTTCACATTGGACTTCTTTTCCTCTTCATTTACGTGTTCGATTGGGGGATATATGGTGCAGCTATAGCTTTTGACATTACAGGTTGGGAAATTGTGATTGCTGAGCTTGTTTATATACTTGGTTGGTGTAACGAAGGGTGGAGCGGGTTTTCATGGTTGGCTTTCAAAGACATTTGGGCCTTTGTGAAGCTTTCGCTTGAATCAGCTGTCATGCTTTGCCTTGAGATTTGGTATATGATGAGTATCATCATTCTTACTGGCCATCTTGACAATGCGGTCATTGCTCTTGATTCACTTTCTATTTGCATGAATGTCAATGGATGGGAAGCAATGCTGTTCATTGGAATCAATGCTGCTATTAGTGTTCGAGTTTCTAATGAACTTGGACTAGGGCGTCCGAGAGCAGCTAAGTACTCAGTCTATGTGACAGTGGCTCAATCTCTGCTGATTGGAATTTTTTGCATGGTTGTTATCTTGATTACCAAAGACTACTTCTCTGTCCTCTTCACAAGCAATGCCGAATTGCAAGCAGCTGTTTCAAAGCTAGCATGGCTTCTCGGAATCACCATGGTTCTCAACAGTGTCCAGCCTGTGATTTCAGGTATCAATAAATCGTGTTTGTTGGCTTCCATCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

404

Amino Acids

44.43

Weight (kDa)

5.12

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 40 - 198 9.8e-32 MatE
MatE PF01554 261 - 397 1.1e-18 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 851, 969
Acc36I ACCTGC 1 cut(s) 374
AccB1I GGYRCC 2 cut(s) 7, 149
AccBSI CCGCTC 1 cut(s) 720
AciI CCGC 6 cut(s) 11, 204, 440, 449, 720, 851
AclWI GGATC 3 cut(s) 356, 466, 563
AcoI YGGCCR 3 cut(s) 178, 421, 835
AcsI RAATTY 1 cut(s) 1029
AcyI GRCGYC 2 cut(s) 8, 970
AfaI GTAC 2 cut(s) 235, 989
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 3 cut(s) 742, 776, 1116
AjiI CACGTC 1 cut(s) 81
AjnI CCWGG 1 cut(s) 444
AjuI GAANNNNNNNTTGG 2 cut(s) 1052, 1084
Alw21I GWGCWC 2 cut(s) 201, 274
Alw26I GTCTC 3 cut(s) 166, 214, 272
Alw44I GTGCAC 1 cut(s) 270
AlwI GGATC 3 cut(s) 356, 466, 563
Ama87I CYCGRG 1 cut(s) 69
AoxI GGCC 5 cut(s) 116, 178, 421, 753, 835
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 5 cut(s) 13, 635, 929, 980, 1106
ApoI RAATTY 1 cut(s) 1029
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 3 cut(s) 116, 442, 753
AsuHPI GGTGA 5 cut(s) 61, 88, 121, 451, 1132
AsuNHI GCTAGC 1 cut(s) 1120
AvaI CYCGRG 1 cut(s) 69
AvaII GGWCC 1 cut(s) 442
BaeGI GKGCMC 1 cut(s) 274
BalI TGGCCA 2 cut(s) 423, 837
BanI GGYRCC 2 cut(s) 7, 149
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 201, 274
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 4 cut(s) 647, 916, 992, 1118
BccI CCATC 4 cut(s) 503, 846, 891, 1214
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 1 cut(s) 446
BcoDI GTCTC 3 cut(s) 166, 214, 272
BfaI CTAG 3 cut(s) 275, 965, 1121
BfmI CTRYAG 3 cut(s) 120, 521, 639
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 374
BisI GCNGC 6 cut(s) 11, 14, 636, 930, 981, 1107
BlpI GCTNAGC 1 cut(s) 678
BlsI GCNGC 6 cut(s) 12, 15, 637, 931, 982, 1108
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 1 cut(s) 442
BmeT110I CYCGRG 1 cut(s) 69
BmgBI CACGTC 1 cut(s) 81
BmgT120I GGNCC 3 cut(s) 116, 442, 753
BmiI GGNNCC 2 cut(s) 9, 151
BmrFI CCNGG 1 cut(s) 446
BmtI GCTAGC 1 cut(s) 1124
Bpu10I CCTNAGC 1 cut(s) 477
Bpu1102I GCTNAGC 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 818
BsaAI YACGTR 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 818
BsaHI GRCGYC 2 cut(s) 8, 970
BsaJI CCNNGG 1 cut(s) 1143
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 97, 127, 146, 176
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 838
Bse3DI GCAATG 3 cut(s) 855, 912, 1096
Bse8I GATNNNNATC 1 cut(s) 818
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 1 cut(s) 1143
BseGI GGATG 2 cut(s) 902, 1206
BseJI GATNNNNATC 1 cut(s) 818
BseMI GCAATG 3 cut(s) 855, 912, 1096
BseMII CTCAG 3 cut(s) 491, 669, 1005
BseNI ACTGG 1 cut(s) 838
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 4 cut(s) 647, 916, 992, 1118
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 5 cut(s) 118, 180, 423, 755, 837
BshNI GGYRCC 2 cut(s) 7, 149
BsiHKAI GWGCWC 2 cut(s) 201, 274
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 1 cut(s) 47
BsmAI GTCTC 3 cut(s) 166, 214, 272
BsmBI CGTCTC 2 cut(s) 166, 214
BsnI GGCC 5 cut(s) 118, 180, 423, 755, 837
BsoBI CYCGRG 1 cut(s) 69
Bsp1286I GDGCHC 2 cut(s) 201, 274
Bsp143I GATC 3 cut(s) 348, 471, 555
Bsp1720I GCTNAGC 1 cut(s) 678
Bsp19I CCATGG 1 cut(s) 1143
BspACI CCGC 6 cut(s) 11, 204, 440, 449, 720, 851
BspANI GGCC 5 cut(s) 118, 180, 423, 755, 837
BspCNI CTCAG 3 cut(s) 490, 670, 1004
BspLI GGNNCC 2 cut(s) 9, 151
BspMAI CTGCAG 1 cut(s) 525
BspMI ACCTGC 1 cut(s) 374
BspOI GCTAGC 1 cut(s) 1124
BspPI GGATC 3 cut(s) 356, 466, 563
BspT107I GGYRCC 2 cut(s) 7, 149
BsrBI CCGCTC 1 cut(s) 720
BsrDI GCAATG 3 cut(s) 855, 912, 1096
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 1 cut(s) 838
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 1143
BssMI GATC 3 cut(s) 348, 471, 555
BssNI GRCGYC 2 cut(s) 8, 970
BssT1I CCWWGG 1 cut(s) 1143
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 4 cut(s) 94, 463, 1006, 1157
Bst6I CTCTTC 2 cut(s) 605, 1085
BstACI GRCGYC 2 cut(s) 8, 970
BstBAI YACGTR 1 cut(s) 610
BstC8I GCNNGC 4 cut(s) 66, 525, 1104, 1122
BstDEI CTNAG 4 cut(s) 477, 678, 984, 991
BstDSI CCRYGG 1 cut(s) 1143
BstF5I GGATG 2 cut(s) 902, 1206
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 3 cut(s) 351, 474, 558
BstMAI GTCTC 3 cut(s) 166, 214, 272
BstMBI GATC 3 cut(s) 348, 471, 555
BstMWI GCNNNNNNNGC 6 cut(s) 147, 302, 446, 641, 857, 1126
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 2 cut(s) 322, 337
BstSCI CCNGG 1 cut(s) 444
BstSFI CTRYAG 3 cut(s) 120, 521, 639
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 4 cut(s) 647, 916, 992, 1118
BsuRI GGCC 5 cut(s) 118, 180, 423, 755, 837
BtgI CCRYGG 1 cut(s) 1143
BtrI CACGTC 1 cut(s) 81
BtsCI GGATG 2 cut(s) 902, 1206
BtsIMutI CAGTG 3 cut(s) 99, 1011, 1162
BveI ACCTGC 1 cut(s) 374
Cac8I GCNNGC 4 cut(s) 66, 525, 1104, 1122
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 3 cut(s) 116, 442, 753
CseI GACGC 1 cut(s) 959
Csp6I GTAC 2 cut(s) 234, 988
CviQI GTAC 2 cut(s) 234, 988
DdeI CTNAG 4 cut(s) 477, 678, 984, 991
DinI GGCGCC 1 cut(s) 9
DpnI GATC 3 cut(s) 350, 473, 557
DpnII GATC 3 cut(s) 348, 471, 555
DrdI GACNNNNNNGTC 2 cut(s) 851, 969
DseDI GACNNNNNNGTC 2 cut(s) 851, 969
EaeI YGGCCR 3 cut(s) 178, 421, 835
Eam1104I CTCTTC 2 cut(s) 605, 1085
EarI CTCTTC 2 cut(s) 605, 1085
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 1143
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 442
Eco53kI GAGCTC 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 1143
EcoT38I GRGCYC 1 cut(s) 201
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 1143
Esp3I CGTCTC 2 cut(s) 166, 214
FauI CCCGC 2 cut(s) 442, 713
Fnu4HI GCNGC 6 cut(s) 11, 14, 636, 930, 981, 1107
FokI GGATG 2 cut(s) 909, 1193
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 6 cut(s) 11, 14, 636, 930, 981, 1107
FspBI CTAG 3 cut(s) 275, 965, 1121
GlaI GCGC 1 cut(s) 9
GluI GCNGC 6 cut(s) 11, 14, 636, 930, 981, 1107
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 5 cut(s) 118, 180, 423, 755, 837
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 959
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 2 cut(s) 8, 970
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 3 cut(s) 55, 413, 764
HinfI GANTC 5 cut(s) 561, 776, 866, 921, 1137
HpaI GTTAAC 1 cut(s) 316
HpaII CCGG 1 cut(s) 47
HphI GGTGA 5 cut(s) 61, 88, 121, 451, 1132
Hpy166II GTNNAC 3 cut(s) 272, 316, 580
Hpy188I TCNGA 4 cut(s) 188, 560, 975, 1136
Hpy188III TCNNGA 4 cut(s) 71, 842, 863, 1051
Hpy8I GTNNAC 3 cut(s) 272, 316, 580
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 3 cut(s) 136, 247, 704
HpyCH4III ACNGT 4 cut(s) 94, 463, 1006, 1157
HpyCH4IV ACGT 2 cut(s) 80, 609
HpyCH4V TGCA 8 cut(s) 141, 272, 337, 523, 635, 882, 1038, 1102
HpyF10VI GCNNNNNNNGC 6 cut(s) 147, 302, 446, 641, 857, 1126
HpyF3I CTNAG 4 cut(s) 477, 678, 984, 991
HpySE526I ACGT 2 cut(s) 80, 609
Hsp92I GRCGYC 2 cut(s) 8, 970
HspAI GCGC 1 cut(s) 8
KasI GGCGCC 1 cut(s) 7
KspAI GTTAAC 1 cut(s) 316
Kzo9I GATC 3 cut(s) 348, 471, 555
LmnI GCTCC 3 cut(s) 26, 302, 717
Lsp1109I GCAGC 4 cut(s) 647, 916, 992, 1118
MaeI CTAG 3 cut(s) 275, 965, 1121
MaeII ACGT 2 cut(s) 80, 609
MaeIII GTNAC 5 cut(s) 31, 49, 76, 704, 1000
MalI GATC 3 cut(s) 350, 473, 557
MbiI CCGCTC 1 cut(s) 720
MboI GATC 3 cut(s) 348, 471, 555
MboII GAAGA 2 cut(s) 592, 1072
MhlI GDGCHC 2 cut(s) 201, 274
MlsI TGGCCA 2 cut(s) 423, 837
MluCI AATT 4 cut(s) 499, 666, 1029, 1097
MluNI TGGCCA 2 cut(s) 423, 837
Mly113I GGCGCC 1 cut(s) 8
MnlI CCTC 5 cut(s) 67, 99, 486, 608, 1088
Mox20I TGGCCA 2 cut(s) 423, 837
MscI TGGCCA 2 cut(s) 423, 837
MseI TTAA 3 cut(s) 60, 315, 410
MslI CAYNNNNRTG 1 cut(s) 608
Msp20I TGGCCA 2 cut(s) 423, 837
MspA1I CMGCKG 3 cut(s) 13, 782, 1109
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 446
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 6 cut(s) 147, 302, 446, 641, 857, 1126
NarI GGCGCC 1 cut(s) 8
NcoI CCATGG 1 cut(s) 1143
NdeII GATC 3 cut(s) 348, 471, 555
NheI GCTAGC 1 cut(s) 1120
NlaIV GGNNCC 2 cut(s) 9, 151
NmuCI GTSAC 4 cut(s) 31, 49, 76, 1000
NspI RCATGY 2 cut(s) 322, 337
PfeI GAWTC 5 cut(s) 561, 776, 866, 921, 1137
PkrI GCNGC 6 cut(s) 12, 15, 637, 931, 982, 1108
PluTI GGCGCC 1 cut(s) 11
Ppu21I YACGTR 1 cut(s) 610
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 444
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 2 cut(s) 9, 151
PspPI GGNCC 3 cut(s) 116, 442, 753
PstI CTGCAG 1 cut(s) 525
PvuII CAGCTG 2 cut(s) 782, 1109
RsaI GTAC 2 cut(s) 235, 989
RsaNI GTAC 2 cut(s) 234, 988
RseI CAYNNNNRTG 1 cut(s) 608
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 3 cut(s) 60, 315, 410
SatI GCNGC 6 cut(s) 11, 14, 636, 930, 981, 1107
Sau3AI GATC 3 cut(s) 348, 471, 555
Sau96I GGNCC 3 cut(s) 116, 442, 753
SbfI CCTGCAGG 1 cut(s) 525
ScaI AGTACT 1 cut(s) 989
ScrFI CCNGG 1 cut(s) 446
SdaI CCTGCAGG 1 cut(s) 525
SduI GDGCHC 2 cut(s) 201, 274
SfcI CTRYAG 3 cut(s) 120, 521, 639
SfoI GGCGCC 1 cut(s) 9
SinI GGWCC 1 cut(s) 442
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
Sse8387I CCTGCAGG 1 cut(s) 525
Sse9I AATT 4 cut(s) 499, 666, 1029, 1097
SsiI CCGC 6 cut(s) 11, 204, 440, 449, 720, 851
SspDI GGCGCC 1 cut(s) 7
SspMI CTAG 3 cut(s) 275, 965, 1121
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 4 cut(s) 94, 463, 1006, 1157
TaiI ACGT 2 cut(s) 83, 612
TaqI TCGA 2 cut(s) 615, 943
TasI AATT 4 cut(s) 499, 666, 1029, 1097
TatI WGTACW 1 cut(s) 987
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 5 cut(s) 561, 776, 866, 921, 1137
Tru1I TTAA 3 cut(s) 60, 315, 410
Tru9I TTAA 3 cut(s) 60, 315, 410
TscAI CASTG 3 cut(s) 99, 1011, 1162
TseFI GTSAC 4 cut(s) 31, 49, 76, 1000
TseI GCWGC 5 cut(s) 13, 635, 929, 980, 1106
Tsp45I GTSAC 4 cut(s) 31, 49, 76, 1000
TspDTI ATGAA 7 cut(s) 241, 553, 592, 717, 899, 904, 969
TspGWI ACGGA 4 cut(s) 39, 50, 148, 214
TspRI CASTG 3 cut(s) 99, 1011, 1162
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 442
XapI RAATTY 1 cut(s) 1029
XceI RCATGY 2 cut(s) 322, 337
XspI CTAG 3 cut(s) 275, 965, 1121
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.