pycom01g21670

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000970
Physical Location & Seq
Reverse (-)
19609290 .. 19613302
4013 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21670.7

Sequence Viewer

Length: 1509 bp
ATGGAGGAGCCGCTGCTCGACACAGCCTCCGGCAGCACCACCGAGTATAGCAATAAGCAGCTTATATACGAAGGTGAGAGCGAAGACTATGCCCCGGTGAGGAGCTTTGGTGCGTTGCGGCATATGTTTTGGATAGAGACGGTGAAGCTGTGGCAAATAGGCGGTTCGGCGGTTATCACTATCATGTGTATGTATGGGACTAACTCAGTCATCGTGCTCTTTGCCGGTCATCTCGGAACTATTGAGCTTTCCGCCATCTCCATTTCTCTTTCTGTCATTTCCACCTTCACTTATGGATTCATGCTTGGGATGGGGAGTGCTCTGGAAACCCTGTGTGGACAAGCATTTGGTGCTGGACAAGTTCACATGCTTGGTATCTACATGCAACGATCATGTATAATTCTATTCGTAACCTCCTTCTTACTGTTGCCGATTTACATCTTTGCCACACCGATTCTAAAGTGTCTAGGCCAAGAAGACGACATAGCAAATGAAGCCGGAAAATACACACTCCAAATAATCCCCCAGTTGTTCTCACTTGCCATAAATTTCCCAGCTCAAAAGTTCCTCCAGGCTCAGAGGAAGGTGAAGGTACTGGCATGGATTGCAGTGTTGGCCCTGGTCATACACATTGGAATGCTAGCGCTCTTCATATATGTGTTTGATTGGGGCATATCGGGGGCAGCTGTGTCATTTAACATCACAAGATGGGGGATTTCAATTGCTCAAGTTGTCTATATTATGGGTTGGTGTAAGGAGGGTTGGACTGGATTTTCATGGCTGGCATTCAATGAGATTTGGGCATTTGTGAGACTTTCTCTTGCCTCTGCAGTCATGCTCTGCCTGGAAATTTGGTACATGATGAGTATACTCCTTCTCACAGGCGGTCTTTCTGATGCAGTTATAGCCGTTGGTTCCCTTTCTATTTGCATGAACATCAACGGGTGGGAAGGAATGTTGTTTGTTGGACTAAACGCCGCAATAAGCGTTCGCGTCTCCAATGAGCTTGGGTTAAAACGTCCTAGAGCAGCTAAATACGCTGTTTATGTGACAGTAGCTCAGTCTCTCTTAATTGGGCTTCTTTTCATGGTTATTATTTTAATAACCAAAGACTATTTTGCCGTAATTTTCACAAGTGACAAAGAGTTGCAACAAGCAGTTGCTAAACTTGCATACCTTCTTGGTATAACTATGGTGCTCAACAGTGTTCAACCAGTAATATCAGGTGTTGCAATTGGTGGTGGATGGCAGGCAATGGTAGCTTATATAAACTTGGGTTGTTACTACATCTTTGGACTTCCTCTTGGATACGTTCTTTGCCATGTTGCAAATTTAGGAGTCGCGGGACTTTGGGGGGGCATGATATGTGGAACTGCTCTCCAAACCATCCTTCTGCTAATTATACTTTACAAAACAAACTGGAACAAAGAGGTGGAGCAAGCAACAAATCGTGTCCGGAAGTGGGGAGGGCAAGACATCAAAACTGCCAACGGAGCTAAAAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

503

Amino Acids

54.91

Weight (kDa)

7.49

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 868
AccII CGCG 2 cut(s) 993, 1343
AccIII TCCGGA 1 cut(s) 1455
AciI CCGC 8 cut(s) 11, 118, 162, 170, 252, 885, 978, 1343
AcsI RAATTY 3 cut(s) 547, 849, 1330
AfaI GTAC 3 cut(s) 594, 857, 1504
AfeI AGCGCT 1 cut(s) 645
AfiI CCNNNNNNNGG 2 cut(s) 99, 1462
AgsI TTSAA 3 cut(s) 720, 790, 1211
AjnI CCWGG 3 cut(s) 570, 618, 843
AjuI GAANNNNNNNTTGG 2 cut(s) 1374, 1406
Alw21I GWGCWC 3 cut(s) 219, 322, 1200
Alw26I GTCTC 4 cut(s) 131, 805, 1000, 1068
Aor13HI TCCGGA 1 cut(s) 1455
Aor51HI AGCGCT 1 cut(s) 645
AoxI GGCC 2 cut(s) 469, 615
ApeKI GCWGC 5 cut(s) 13, 33, 58, 683, 1028
ApoI RAATTY 3 cut(s) 547, 849, 1330
AspLEI GCGC 1 cut(s) 646
AspS9I GGNCC 1 cut(s) 616
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 4 cut(s) 86, 109, 154, 598
AsuNHI GCTAGC 1 cut(s) 640
BbsI GAAGAC 2 cut(s) 90, 483
Bbv12I GWGCWC 3 cut(s) 219, 322, 1200
BbvI GCAGC 4 cut(s) 45, 70, 695, 1040
BccI CCATC 5 cut(s) 263, 304, 702, 1239, 1394
BceAI ACGGC 2 cut(s) 893, 1106
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 3 cut(s) 572, 620, 845
BciVI GTATCC 1 cut(s) 1301
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 4 cut(s) 131, 805, 1000, 1068
BfaI CTAG 3 cut(s) 467, 641, 1023
BfmI CTRYAG 1 cut(s) 828
BfoI RGCGCY 1 cut(s) 647
BfuI GTATCC 1 cut(s) 1301
BisI GCNGC 8 cut(s) 11, 14, 34, 59, 119, 684, 978, 1029
BlsI GCNGC 8 cut(s) 12, 15, 35, 60, 120, 685, 979, 1030
BmcAI AGTACT 1 cut(s) 1504
Bme1390I CCNGG 4 cut(s) 95, 572, 620, 845
BmgT120I GGNCC 1 cut(s) 616
BmiI GGNNCC 2 cut(s) 9, 916
BmrFI CCNGG 4 cut(s) 95, 572, 620, 845
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 1 cut(s) 886
BmtI GCTAGC 1 cut(s) 644
BmuI ACTGGG 1 cut(s) 520
BpiI GAAGAC 2 cut(s) 90, 483
BplI GAGNNNNNCTC 2 cut(s) 802, 834
BpmI CTGGAG 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 711
BpuMI CCSGG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 93, 618
BsaWI WCCGGW 1 cut(s) 1455
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 94, 124
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 1462
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 5 cut(s) 526, 600, 772, 1214, 1424
Bse3DI GCAATG 1 cut(s) 1260
Bse8I GATNNNNATC 1 cut(s) 437
BseAI TCCGGA 1 cut(s) 1455
BseBI CCWGG 3 cut(s) 572, 620, 845
BseDI CCNNGG 2 cut(s) 93, 618
BseGI GGATG 3 cut(s) 315, 1250, 1386
BseJI GATNNNNATC 1 cut(s) 437
BseLI CCNNNNNNNGG 2 cut(s) 99, 1462
BseMI GCAATG 1 cut(s) 1260
BseMII CTCAG 3 cut(s) 219, 590, 1073
BseNI ACTGG 5 cut(s) 526, 600, 772, 1214, 1424
BseRI GAGGAG 2 cut(s) 20, 115
BseXI GCAGC 4 cut(s) 45, 70, 695, 1040
BseYI CCCAGC 1 cut(s) 553
Bsh1236I CGCG 2 cut(s) 993, 1343
BshFI GGCC 2 cut(s) 471, 617
BsiHKAI GWGCWC 3 cut(s) 219, 322, 1200
BsiSI CCGG 5 cut(s) 30, 95, 225, 498, 1456
BslFI GGGAC 2 cut(s) 211, 1359
BslI CCNNNNNNNGG 2 cut(s) 99, 1462
BsmAI GTCTC 4 cut(s) 131, 805, 1000, 1068
BsmBI CGTCTC 2 cut(s) 131, 1000
BsmFI GGGAC 2 cut(s) 211, 1359
BsmI GAATGC 2 cut(s) 642, 785
BsnI GGCC 2 cut(s) 471, 617
Bsp1286I GDGCHC 3 cut(s) 219, 322, 1200
Bsp13I TCCGGA 1 cut(s) 1455
Bsp143I GATC 1 cut(s) 389
BspACI CCGC 8 cut(s) 11, 118, 162, 170, 252, 885, 978, 1343
BspANI GGCC 2 cut(s) 471, 617
BspCNI CTCAG 3 cut(s) 218, 589, 1072
BspEI TCCGGA 1 cut(s) 1455
BspFNI CGCG 2 cut(s) 993, 1343
BspLI GGNNCC 2 cut(s) 9, 916
BspMAI CTGCAG 1 cut(s) 832
BspOI GCTAGC 1 cut(s) 644
BspQI GCTCTTC 1 cut(s) 653
BsrDI GCAATG 1 cut(s) 1260
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 5 cut(s) 526, 600, 772, 1214, 1424
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 2 cut(s) 93, 618
BssMI GATC 1 cut(s) 389
BssNAI GTATAC 1 cut(s) 869
Bst1107I GTATAC 1 cut(s) 869
Bst2UI CCWGG 3 cut(s) 572, 620, 845
Bst4CI ACNGT 4 cut(s) 142, 426, 1054, 1205
Bst6I CTCTTC 1 cut(s) 653
BstAPI GCANNNNNTGC 2 cut(s) 350, 605
BstC8I GCNNGC 4 cut(s) 642, 783, 1251, 1440
BstDEI CTNAG 3 cut(s) 205, 576, 1059
BstF5I GGATG 3 cut(s) 315, 1250, 1386
BstFNI CGCG 2 cut(s) 993, 1343
BstH2I RGCGCY 1 cut(s) 647
BstHHI GCGC 1 cut(s) 646
BstKTI GATC 1 cut(s) 392
BstMAI GTCTC 4 cut(s) 131, 805, 1000, 1068
BstMBI GATC 1 cut(s) 389
BstMWI GCNNNNNNNGC 9 cut(s) 350, 494, 605, 614, 905, 1037, 1169, 1259, 1493
BstNI CCWGG 3 cut(s) 572, 620, 845
BstNSI RCATGY 2 cut(s) 370, 385
BstSCI CCNGG 4 cut(s) 93, 570, 618, 843
BstSFI CTRYAG 1 cut(s) 828
BstUI CGCG 2 cut(s) 993, 1343
BstV1I GCAGC 4 cut(s) 45, 70, 695, 1040
BstV2I GAAGAC 2 cut(s) 90, 483
BstZ17I GTATAC 1 cut(s) 869
BsuI GTATCC 1 cut(s) 1301
BsuRI GGCC 2 cut(s) 471, 617
BtsCI GGATG 3 cut(s) 315, 1250, 1386
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 2 cut(s) 615, 1210
Cac8I GCNNGC 4 cut(s) 642, 783, 1251, 1440
CfoI GCGC 1 cut(s) 646
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 1 cut(s) 616
CseI GACGC 1 cut(s) 982
Csp6I GTAC 3 cut(s) 593, 856, 1503
CviQI GTAC 3 cut(s) 593, 856, 1503
DdeI CTNAG 3 cut(s) 205, 576, 1059
DpnI GATC 1 cut(s) 391
DpnII GATC 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EciI GGCGGA 1 cut(s) 241
Eco47III AGCGCT 1 cut(s) 645
EcoRII CCWGG 3 cut(s) 570, 618, 843
Esp3I CGTCTC 2 cut(s) 131, 1000
FaqI GGGAC 2 cut(s) 211, 1359
FauI CCCGC 1 cut(s) 1336
FauNDI CATATG 1 cut(s) 123
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 8 cut(s) 11, 14, 34, 59, 119, 684, 978, 1029
FokI GGATG 3 cut(s) 322, 1257, 1373
Fsp4HI GCNGC 8 cut(s) 11, 14, 34, 59, 119, 684, 978, 1029
FspBI CTAG 3 cut(s) 467, 641, 1023
GlaI GCGC 1 cut(s) 645
GluI GCNGC 8 cut(s) 11, 14, 34, 59, 119, 684, 978, 1029
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 1 cut(s) 554
HaeII RGCGCY 1 cut(s) 647
HaeIII GGCC 2 cut(s) 471, 617
HapII CCGG 5 cut(s) 30, 95, 225, 498, 1456
HgaI GACGC 1 cut(s) 982
HhaI GCGC 1 cut(s) 646
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HinfI GANTC 3 cut(s) 297, 454, 1338
HpaII CCGG 5 cut(s) 30, 95, 225, 498, 1456
HphI GGTGA 4 cut(s) 86, 109, 154, 598
Hpy166II GTNNAC 3 cut(s) 338, 364, 869
Hpy188I TCNGA 3 cut(s) 236, 579, 895
Hpy188III TCNNGA 2 cut(s) 323, 1456
Hpy8I GTNNAC 3 cut(s) 338, 364, 869
HpyAV CCTTC 9 cut(s) 65, 295, 427, 577, 583, 884, 944, 1187, 1400
HpyCH4III ACNGT 4 cut(s) 142, 426, 1054, 1205
HpyCH4IV ACGT 2 cut(s) 1018, 1311
HpyCH4V TGCA 9 cut(s) 385, 608, 830, 899, 930, 1150, 1172, 1232, 1328
HpyF10VI GCNNNNNNNGC 9 cut(s) 350, 494, 605, 614, 905, 1037, 1169, 1259, 1493
HpyF3I CTNAG 3 cut(s) 205, 576, 1059
HpySE526I ACGT 2 cut(s) 1018, 1311
HspAI GCGC 1 cut(s) 644
Kpn2I TCCGGA 1 cut(s) 1455
Kzo9I GATC 1 cut(s) 389
LguI GCTCTTC 1 cut(s) 653
LmnI GCTCC 4 cut(s) 7, 102, 1435, 1493
Lsp1109I GCAGC 4 cut(s) 45, 70, 695, 1040
LweI GCATC 1 cut(s) 886
MaeI CTAG 3 cut(s) 467, 641, 1023
MaeII ACGT 2 cut(s) 1018, 1311
MaeIII GTNAC 4 cut(s) 409, 1048, 1136, 1280
MalI GATC 1 cut(s) 391
MboI GATC 1 cut(s) 389
MboII GAAGA 3 cut(s) 95, 488, 640
MfeI CAATTG 2 cut(s) 720, 1233
MhlI GDGCHC 3 cut(s) 219, 322, 1200
MluCI AATT 9 cut(s) 399, 547, 720, 849, 1071, 1125, 1233, 1330, 1398
MlyI GAGTC 1 cut(s) 1347
MmeI TCCRAC 2 cut(s) 743, 946
MroI TCCGGA 1 cut(s) 1455
MseI TTAA 4 cut(s) 696, 1013, 1070, 1100
MslI CAYNNNNRTG 4 cut(s) 182, 188, 635, 656
MspA1I CMGCKG 2 cut(s) 13, 686
MspI CCGG 5 cut(s) 30, 95, 225, 498, 1456
MspR9I CCNGG 4 cut(s) 95, 572, 620, 845
MunI CAATTG 2 cut(s) 720, 1233
Mva1269I GAATGC 2 cut(s) 642, 785
MvaI CCWGG 3 cut(s) 572, 620, 845
MvnI CGCG 2 cut(s) 993, 1343
MwoI GCNNNNNNNGC 9 cut(s) 350, 494, 605, 614, 905, 1037, 1169, 1259, 1493
NciI CCSGG 1 cut(s) 95
NdeI CATATG 1 cut(s) 123
NdeII GATC 1 cut(s) 389
NheI GCTAGC 1 cut(s) 640
NlaIV GGNNCC 2 cut(s) 9, 916
NmuCI GTSAC 2 cut(s) 1048, 1136
NspI RCATGY 2 cut(s) 370, 385
PciSI GCTCTTC 1 cut(s) 653
PctI GAATGC 2 cut(s) 642, 785
PfeI GAWTC 2 cut(s) 297, 454
PkrI GCNGC 8 cut(s) 12, 15, 35, 60, 120, 685, 979, 1030
PleI GAGTC 1 cut(s) 1346
PpsI GAGTC 1 cut(s) 1346
Psp6I CCWGG 3 cut(s) 570, 618, 843
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 3 cut(s) 570, 618, 843
PspN4I GGNNCC 2 cut(s) 9, 916
PspPI GGNCC 1 cut(s) 616
PstI CTGCAG 1 cut(s) 832
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 3 cut(s) 594, 857, 1504
RsaNI GTAC 3 cut(s) 593, 856, 1503
RseI CAYNNNNRTG 4 cut(s) 182, 188, 635, 656
SapI GCTCTTC 1 cut(s) 653
SaqAI TTAA 4 cut(s) 696, 1013, 1070, 1100
SatI GCNGC 8 cut(s) 11, 14, 34, 59, 119, 684, 978, 1029
Sau3AI GATC 1 cut(s) 389
Sau96I GGNCC 1 cut(s) 616
ScaI AGTACT 1 cut(s) 1504
SchI GAGTC 1 cut(s) 1347
ScrFI CCNGG 4 cut(s) 95, 572, 620, 845
SduI GDGCHC 3 cut(s) 219, 322, 1200
SfaNI GCATC 1 cut(s) 886
SfcI CTRYAG 1 cut(s) 828
SmiMI CAYNNNNRTG 4 cut(s) 182, 188, 635, 656
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
Sse9I AATT 9 cut(s) 399, 547, 720, 849, 1071, 1125, 1233, 1330, 1398
SsiI CCGC 8 cut(s) 11, 118, 162, 170, 252, 885, 978, 1343
SspMI CTAG 3 cut(s) 467, 641, 1023
StyD4I CCNGG 4 cut(s) 93, 570, 618, 843
TaaI ACNGT 4 cut(s) 142, 426, 1054, 1205
TaiI ACGT 2 cut(s) 1021, 1314
TaqI TCGA 1 cut(s) 18
TasI AATT 9 cut(s) 399, 547, 720, 849, 1071, 1125, 1233, 1330, 1398
TatI WGTACW 1 cut(s) 1502
TauI GCSGC 3 cut(s) 13, 121, 980
TfiI GAWTC 2 cut(s) 297, 454
Tru1I TTAA 4 cut(s) 696, 1013, 1070, 1100
Tru9I TTAA 4 cut(s) 696, 1013, 1070, 1100
TscAI CASTG 2 cut(s) 615, 1210
TseFI GTSAC 2 cut(s) 1048, 1136
TseI GCWGC 5 cut(s) 13, 33, 58, 683, 1028
Tsp45I GTSAC 2 cut(s) 1048, 1136
TspDTI ATGAA 6 cut(s) 289, 507, 640, 765, 947, 1075
TspGWI ACGGA 1 cut(s) 1506
TspRI CASTG 2 cut(s) 615, 1210
XapI RAATTY 3 cut(s) 547, 849, 1330
XceI RCATGY 2 cut(s) 370, 385
XmiI GTMKAC 1 cut(s) 868
XspI CTAG 3 cut(s) 467, 641, 1023
ZrmI AGTACT 1 cut(s) 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.