MD07G1277200.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000970
Physical Location & Seq
Reverse (-)
34250462 .. 34252899
2438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1277200.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGAGGAGCAGGAGCCGCCGCTTCACACAGCTGCTGCCGGCGCCACTGAGTTGAGCAATAAACCTCCTCTTTATGTAGGCGGGAACGAAGACTATGCGCAGGTGAAGAGCTTTGATGCGCTCCGAAGTATGTTTTGGATAGAGACGGTGAAGTTGTGGCAAATGGCCGGTTCGGCAGTGATCACCATCATATGTATGTACGGGACTAACGCAGTCATCCTGCTCTTTGCCGGTCATCTAGGAACTATCCAGCTCTCCGCTATCTCTATTTCTTTAGCCGTGATTTCCACATTCACTGATGGACTGATGCTTGGGATGGCGAGTGCTCTAGAGACCTTGTGTGGACAAGCATTTGGCGCTGGACAAGTTCATATGCTTGGGATTTACCTGCAACGATCCTGGATAATCCTATTTGTAACCACCCTCTTCATTTTGCCGATTTACATCTTCGCGGCACCAATTTTAAAGTTGCTAGGCCAAGAAGACGACATAGCGAATCTAGCAGGAAAATTCACACTCCAAATAATCCCCCAATTGTTCTCACTTGCCATAAATTTCCCAGCTCAGAAGTTCCTCCAGGCTCAGAGGAAGGTGAAGATGCTGGCATGGATTGCAATGTTGGCCCTGGTCATACACATTGGAATGATAGCGCTCTTCATGTATGTGTTTAATTGGGGCACATTGGGGGCAGCTGTGTCATTTAACATCACAAGATGGGCGATTGCAATTGCACAGGTTGTGTATATTATGGGTTGGTGCAAAGAGGGTTGGACTGGATTTTCATGGATGGCATTGAATGAGATGTGGGCGTTTGTGAGACTTTCTCTTGCGTCTGCGGCCATGCTCTGCCTTGAGATTTGGTACATGATGAGTATACTCATTCTCACAGGCCGTCTTCCTAACGCAGTTATAGCTCTTGGTTCCCTTTCTATTTGCATGAACATCAACGGGTGGGAGGCAATGTTGTTTGTTGGAATCTATATTGCAATAAGTGTTCGTGTTTCCAATGAGCTTGGGTCAGGACGTCCTAGGGCAACAAAATACTCTATTTATGTGACAGTACTTCAGTCTCTCCTAATTGGAATTCTTTTCATGACTATTATTTTGATAACTAAAGATGACTTCGCCATGATTTTCACAAGTGACAAGGAGTTGCAACAAGCGGTTTCTAAGTTGGCATATCTTCTTGGTATAACTATGGTGCTCAACAGTGTCCAACTAGTAATCTCAGGTGTTGCAGTTGGATGTGGATGGCAAACAATGGTTGCCTACATAAACTTGGGTTGTTACTACATTTTTGGTCTTCCTTTTGGATACCTTCTTTGCCATGTTGCAAACTGGGGAGTCAAGGGATTCTGGGTGGGCATGATATGTGGAACTGTTCTTCAAACCCTACTTCTGCTAATTATACTTTACAAAACCAACTGGAACAAAGAGGTAATCCATAGTTTGTTTCAAAACTTCAACTTCGGTTTTTGCATTTCATTGTTTGTTCATATCAAAACCAAATCGCAGGTGGAGCAAGCATCAACGCGCGTCCGGAAGTGGGGCGGGCAAGGCATCAAAACTGAGAATGGAACTGAAAGTATAGAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

533

Amino Acids

58.86

Weight (kDa)

8.24

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 60 - 214 6.2e-29 MatE
MatE PF01554 278 - 440 3.1e-25 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 91, 1504
AatII GACGTC 1 cut(s) 1027
Acc16I TGCGCA 1 cut(s) 99
Acc36I ACCTGC 3 cut(s) 91, 396, 1504
AccB1I GGYRCC 2 cut(s) 41, 454
AccI GTMKAC 1 cut(s) 874
AccII CGCG 3 cut(s) 452, 1534, 1536
AccIII TCCGGA 1 cut(s) 1539
AciI CCGC 8 cut(s) 17, 20, 81, 258, 452, 836, 1163, 1551
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 2 cut(s) 165, 837
AcsI RAATTY 3 cut(s) 509, 553, 1083
AcuI CTGAAG 1 cut(s) 1049
AcyI GRCGYC 2 cut(s) 42, 1024
AfaI GTAC 3 cut(s) 200, 863, 1062
AfeI AGCGCT 1 cut(s) 651
AfiI CCNNNNNNNGG 1 cut(s) 1546
AgsI TTSAA 4 cut(s) 796, 1388, 1457, 1465
AhlI ACTAGT 1 cut(s) 1219
AjnI CCWGG 3 cut(s) 398, 576, 624
AjuI GAANNNNNNNTTGG 2 cut(s) 118, 150
AluBI AGCT 7 cut(s) 32, 111, 253, 563, 692, 914, 1012
AluI AGCT 7 cut(s) 32, 111, 253, 563, 692, 914, 1012
Alw21I GWGCWC 2 cut(s) 328, 1206
Alw26I GTCTC 4 cut(s) 137, 326, 811, 1074
AlwI GGATC 1 cut(s) 390
AlwNI CAGNNNCTG 1 cut(s) 35
Aor13HI TCCGGA 1 cut(s) 1539
Aor51HI AGCGCT 1 cut(s) 651
AoxI GGCC 5 cut(s) 165, 475, 621, 837, 889
ApeKI GCWGC 3 cut(s) 32, 35, 689
ApoI RAATTY 3 cut(s) 509, 553, 1083
ArsI GACNNNNNNTTYG 2 cut(s) 1087, 1119
AspA2I CCTAGG 1 cut(s) 1028
AspLEI GCGC 6 cut(s) 44, 100, 121, 359, 652, 1536
AspS9I GGNCC 1 cut(s) 622
AsuHPI GGTGA 4 cut(s) 115, 160, 175, 604
AvrII CCTAGG 1 cut(s) 1028
BaeGI GKGCMC 1 cut(s) 680
BanI GGYRCC 2 cut(s) 41, 454
BbsI GAAGAC 4 cut(s) 96, 489, 887, 1295
Bbv12I GWGCWC 2 cut(s) 328, 1206
BbvI GCAGC 3 cut(s) 19, 22, 701
BccI CCATC 6 cut(s) 194, 293, 310, 708, 781, 1245
BceAI ACGGC 2 cut(s) 263, 876
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 3 cut(s) 400, 578, 626
BciVI GTATCC 1 cut(s) 1307
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 4 cut(s) 137, 326, 811, 1074
BcuI ACTAGT 1 cut(s) 1219
BfaI CTAG 6 cut(s) 239, 329, 473, 500, 1029, 1220
BfoI RGCGCY 3 cut(s) 45, 360, 653
BfuAI ACCTGC 3 cut(s) 91, 396, 1504
BfuI GTATCC 1 cut(s) 1307
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 7 cut(s) 17, 20, 33, 36, 453, 690, 837
BlnI CCTAGG 1 cut(s) 1028
BlsI GCNGC 7 cut(s) 18, 21, 34, 37, 454, 691, 838
BmcAI AGTACT 1 cut(s) 1062
Bme1390I CCNGG 3 cut(s) 400, 578, 626
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 4 cut(s) 15, 43, 456, 922
BmrFI CCNGG 3 cut(s) 400, 578, 626
BmrI ACTGGG 1 cut(s) 1348
BmsI GCATC 5 cut(s) 106, 297, 588, 1535, 1569
BmuI ACTGGG 1 cut(s) 1348
BpiI GAAGAC 4 cut(s) 96, 489, 887, 1295
BplI GAGNNNNNCTC 2 cut(s) 808, 840
BpmI CTGGAG 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 872
BsaBI GATNNNNATC 2 cut(s) 185, 443
BsaHI GRCGYC 2 cut(s) 42, 1024
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 624, 1028
BsaWI WCCGGW 1 cut(s) 1539
BsaXI ACNNNNNCTCC 2 cut(s) 947, 977
Bsc4I CCNNNNNNNGG 1 cut(s) 1546
Bse118I RCCGGY 3 cut(s) 38, 167, 230
Bse1I ACTGG 3 cut(s) 778, 1343, 1430
Bse3DI GCAATG 2 cut(s) 621, 966
Bse8I GATNNNNATC 2 cut(s) 185, 443
BseAI TCCGGA 1 cut(s) 1539
BseBI CCWGG 3 cut(s) 400, 578, 626
BseDI CCNNGG 2 cut(s) 624, 1028
BseGI GGATG 5 cut(s) 216, 321, 792, 1250, 1256
BseJI GATNNNNATC 2 cut(s) 185, 443
BseLI CCNNNNNNNGG 1 cut(s) 1546
BseMI GCAATG 2 cut(s) 621, 966
BseMII CTCAG 5 cut(s) 39, 578, 596, 1242, 1560
BseNI ACTGG 3 cut(s) 778, 1343, 1430
BseRI GAGGAG 2 cut(s) 20, 57
BseSI GKGCMC 1 cut(s) 680
BseXI GCAGC 3 cut(s) 19, 22, 701
BseYI CCCAGC 1 cut(s) 559
Bsh1236I CGCG 3 cut(s) 452, 1534, 1536
BshFI GGCC 5 cut(s) 167, 477, 623, 839, 891
BshNI GGYRCC 2 cut(s) 41, 454
BsiHKAI GWGCWC 2 cut(s) 328, 1206
BsiSI CCGG 4 cut(s) 39, 168, 231, 1540
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 1 cut(s) 1546
BsmAI GTCTC 4 cut(s) 137, 326, 811, 1074
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 5 cut(s) 167, 477, 623, 839, 891
Bso31I GGTCTC 1 cut(s) 326
Bsp1286I GDGCHC 3 cut(s) 328, 680, 1206
Bsp13I TCCGGA 1 cut(s) 1539
Bsp143I GATC 2 cut(s) 180, 395
BspACI CCGC 8 cut(s) 17, 20, 81, 258, 452, 836, 1163, 1551
BspANI GGCC 5 cut(s) 167, 477, 623, 839, 891
BspCNI CTCAG 5 cut(s) 40, 577, 595, 1241, 1561
BspEI TCCGGA 1 cut(s) 1539
BspFNI CGCG 3 cut(s) 452, 1534, 1536
BspHI TCATGA 1 cut(s) 1092
BspLI GGNNCC 4 cut(s) 15, 43, 456, 922
BspMI ACCTGC 3 cut(s) 91, 396, 1504
BspPI GGATC 1 cut(s) 390
BspQI GCTCTTC 2 cut(s) 101, 659
BspT107I GGYRCC 2 cut(s) 41, 454
BspTNI GGTCTC 1 cut(s) 326
BsrDI GCAATG 2 cut(s) 621, 966
BsrFI RCCGGY 3 cut(s) 38, 167, 230
BsrI ACTGG 3 cut(s) 778, 1343, 1430
BssAI RCCGGY 3 cut(s) 38, 167, 230
BssECI CCNNGG 2 cut(s) 624, 1028
BssMI GATC 2 cut(s) 180, 395
BssNAI GTATAC 1 cut(s) 875
BssNI GRCGYC 2 cut(s) 42, 1024
BssT1I CCWWGG 1 cut(s) 1028
Bst1107I GTATAC 1 cut(s) 875
Bst2UI CCWGG 3 cut(s) 400, 578, 626
Bst4CI ACNGT 4 cut(s) 148, 1060, 1211, 1381
Bst6I CTCTTC 3 cut(s) 101, 431, 659
BstACI GRCGYC 2 cut(s) 42, 1024
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 4 cut(s) 40, 603, 1524, 1553
BstDEI CTNAG 6 cut(s) 48, 564, 582, 1170, 1228, 1569
BstF5I GGATG 5 cut(s) 216, 321, 792, 1250, 1256
BstFNI CGCG 3 cut(s) 452, 1534, 1536
BstH2I RGCGCY 3 cut(s) 45, 360, 653
BstHHI GCGC 6 cut(s) 44, 100, 121, 359, 652, 1536
BstKTI GATC 2 cut(s) 183, 398
BstMAI GTCTC 4 cut(s) 137, 326, 811, 1074
BstMBI GATC 2 cut(s) 180, 395
BstNI CCWGG 3 cut(s) 400, 578, 626
BstSCI CCNGG 3 cut(s) 398, 576, 624
BstSLI GKGCMC 1 cut(s) 680
BstUI CGCG 3 cut(s) 452, 1534, 1536
BstV1I GCAGC 3 cut(s) 19, 22, 701
BstV2I GAAGAC 4 cut(s) 96, 489, 887, 1295
BstZ17I GTATAC 1 cut(s) 875
BsuI GTATCC 1 cut(s) 1307
BsuRI GGCC 5 cut(s) 167, 477, 623, 839, 891
BtsCI GGATG 5 cut(s) 216, 321, 792, 1250, 1256
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 4 cut(s) 45, 183, 294, 1216
BveI ACCTGC 3 cut(s) 91, 396, 1504
Cac8I GCNNGC 4 cut(s) 40, 603, 1524, 1553
CaiI CAGNNNCTG 1 cut(s) 35
CciI TCATGA 1 cut(s) 1092
CfoI GCGC 6 cut(s) 44, 100, 121, 359, 652, 1536
Cfr10I RCCGGY 3 cut(s) 38, 167, 230
Cfr13I GGNCC 1 cut(s) 622
CseI GACGC 2 cut(s) 819, 1525
Csp6I GTAC 3 cut(s) 199, 862, 1061
CviQI GTAC 3 cut(s) 199, 862, 1061
DdeI CTNAG 6 cut(s) 48, 564, 582, 1170, 1228, 1569
DinI GGCGCC 1 cut(s) 43
DpnI GATC 2 cut(s) 182, 397
DpnII GATC 2 cut(s) 180, 395
DraI TTTAAA 1 cut(s) 465
EaeI YGGCCR 2 cut(s) 165, 837
Eam1104I CTCTTC 3 cut(s) 101, 431, 659
EarI CTCTTC 3 cut(s) 101, 431, 659
Eco130I CCWWGG 1 cut(s) 1028
Eco31I GGTCTC 1 cut(s) 326
Eco47III AGCGCT 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 1049
EcoRI GAATTC 1 cut(s) 1083
EcoRII CCWGG 3 cut(s) 398, 576, 624
EcoT14I CCWWGG 1 cut(s) 1028
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 1028
Esp3I CGTCTC 1 cut(s) 137
FaqI GGGAC 1 cut(s) 217
FauI CCCGC 2 cut(s) 74, 1544
FauNDI CATATG 2 cut(s) 191, 372
FbaI TGATCA 1 cut(s) 180
FblI GTMKAC 1 cut(s) 874
Fnu4HI GCNGC 7 cut(s) 17, 20, 33, 36, 453, 690, 837
FokI GGATG 5 cut(s) 203, 328, 799, 1257, 1263
Fsp4HI GCNGC 7 cut(s) 17, 20, 33, 36, 453, 690, 837
FspBI CTAG 6 cut(s) 239, 329, 473, 500, 1029, 1220
FspI TGCGCA 1 cut(s) 99
GlaI GCGC 6 cut(s) 43, 99, 120, 358, 651, 1535
GluI GCNGC 7 cut(s) 17, 20, 33, 36, 453, 690, 837
GsaI CCCAGC 1 cut(s) 563
GsuI CTGGAG 1 cut(s) 560
HaeII RGCGCY 3 cut(s) 45, 360, 653
HaeIII GGCC 5 cut(s) 167, 477, 623, 839, 891
HapII CCGG 4 cut(s) 39, 168, 231, 1540
HgaI GACGC 2 cut(s) 819, 1525
HhaI GCGC 6 cut(s) 44, 100, 121, 359, 652, 1536
Hin1I GRCGYC 2 cut(s) 42, 1024
Hin6I GCGC 6 cut(s) 42, 98, 119, 357, 650, 1534
HinP1I GCGC 6 cut(s) 42, 98, 119, 357, 650, 1534
HinfI GANTC 4 cut(s) 496, 975, 1344, 1353
HpaII CCGG 4 cut(s) 39, 168, 231, 1540
HphI GGTGA 4 cut(s) 115, 160, 175, 604
Hpy166II GTNNAC 2 cut(s) 344, 875
Hpy188I TCNGA 3 cut(s) 125, 567, 585
Hpy188III TCNNGA 4 cut(s) 329, 1020, 1093, 1540
Hpy8I GTNNAC 2 cut(s) 344, 875
HpyAV CCTTC 2 cut(s) 583, 1328
HpyCH4III ACNGT 4 cut(s) 148, 1060, 1211, 1381
HpyCH4IV ACGT 1 cut(s) 1024
HpyF3I CTNAG 6 cut(s) 48, 564, 582, 1170, 1228, 1569
HpySE526I ACGT 1 cut(s) 1024
Hsp92I GRCGYC 2 cut(s) 42, 1024
HspAI GCGC 6 cut(s) 42, 98, 119, 357, 650, 1534
KasI GGCGCC 1 cut(s) 41
Kpn2I TCCGGA 1 cut(s) 1539
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 180, 395
LguI GCTCTTC 2 cut(s) 101, 659
LmnI GCTCC 4 cut(s) 7, 13, 126, 1519
Lsp1109I GCAGC 3 cut(s) 19, 22, 701
LweI GCATC 5 cut(s) 106, 297, 588, 1535, 1569
MaeI CTAG 6 cut(s) 239, 329, 473, 500, 1029, 1220
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 4 cut(s) 415, 1054, 1142, 1286
MalI GATC 2 cut(s) 182, 397
MboI GATC 2 cut(s) 180, 395
MfeI CAATTG 2 cut(s) 533, 726
MhlI GDGCHC 3 cut(s) 328, 680, 1206
MluCI AATT 9 cut(s) 459, 509, 533, 553, 670, 726, 1077, 1083, 1404
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 1 cut(s) 1353
MmeI TCCRAC 4 cut(s) 749, 952, 1222, 1240
MnlI CCTC 9 cut(s) 75, 78, 434, 579, 584, 757, 949, 1429, 1585
MroI TCCGGA 1 cut(s) 1539
MroNI GCCGGC 1 cut(s) 38
MseI TTAA 3 cut(s) 464, 669, 702
MslI CAYNNNNRTG 3 cut(s) 194, 641, 662
MspA1I CMGCKG 2 cut(s) 32, 692
MspI CCGG 4 cut(s) 39, 168, 231, 1540
MspR9I CCNGG 3 cut(s) 400, 578, 626
MunI CAATTG 2 cut(s) 533, 726
MvaI CCWGG 3 cut(s) 400, 578, 626
MvnI CGCG 3 cut(s) 452, 1534, 1536
NaeI GCCGGC 1 cut(s) 40
NarI GGCGCC 1 cut(s) 42
NdeI CATATG 2 cut(s) 191, 372
NdeII GATC 2 cut(s) 180, 395
NgoMIV GCCGGC 1 cut(s) 38
NlaIV GGNNCC 4 cut(s) 15, 43, 456, 922
NmuCI GTSAC 2 cut(s) 1054, 1142
NsbI TGCGCA 1 cut(s) 99
PagI TCATGA 1 cut(s) 1092
PaqCI CACCTGC 2 cut(s) 91, 1504
PciSI GCTCTTC 2 cut(s) 101, 659
PdiI GCCGGC 1 cut(s) 40
PfeI GAWTC 3 cut(s) 496, 975, 1353
PfoI TCCNGGA 1 cut(s) 398
PkrI GCNGC 7 cut(s) 18, 21, 34, 37, 454, 691, 838
PleI GAGTC 1 cut(s) 1352
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 1352
Psp6I CCWGG 3 cut(s) 398, 576, 624
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 3 cut(s) 398, 576, 624
PspN4I GGNNCC 4 cut(s) 15, 43, 456, 922
PspPI GGNCC 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 35
PvuII CAGCTG 2 cut(s) 32, 692
RsaI GTAC 3 cut(s) 200, 863, 1062
RsaNI GTAC 3 cut(s) 199, 862, 1061
RseI CAYNNNNRTG 3 cut(s) 194, 641, 662
SapI GCTCTTC 2 cut(s) 101, 659
SaqAI TTAA 3 cut(s) 464, 669, 702
SatI GCNGC 7 cut(s) 17, 20, 33, 36, 453, 690, 837
Sau3AI GATC 2 cut(s) 180, 395
Sau96I GGNCC 1 cut(s) 622
ScaI AGTACT 1 cut(s) 1062
SchI GAGTC 1 cut(s) 1353
ScrFI CCNGG 3 cut(s) 400, 578, 626
SduI GDGCHC 3 cut(s) 328, 680, 1206
SfaNI GCATC 5 cut(s) 106, 297, 588, 1535, 1569
SfoI GGCGCC 1 cut(s) 43
SmiMI CAYNNNNRTG 3 cut(s) 194, 641, 662
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
SpeI ACTAGT 1 cut(s) 1219
Sse9I AATT 9 cut(s) 459, 509, 533, 553, 670, 726, 1077, 1083, 1404
SsiI CCGC 8 cut(s) 17, 20, 81, 258, 452, 836, 1163, 1551
SspDI GGCGCC 1 cut(s) 41
SspMI CTAG 6 cut(s) 239, 329, 473, 500, 1029, 1220
StyD4I CCNGG 3 cut(s) 398, 576, 624
StyI CCWWGG 1 cut(s) 1028
TaaI ACNGT 4 cut(s) 148, 1060, 1211, 1381
TaiI ACGT 1 cut(s) 1027
TasI AATT 9 cut(s) 459, 509, 533, 553, 670, 726, 1077, 1083, 1404
TatI WGTACW 1 cut(s) 1060
TauI GCSGC 4 cut(s) 19, 22, 455, 839
TfiI GAWTC 3 cut(s) 496, 975, 1353
Tru1I TTAA 3 cut(s) 464, 669, 702
Tru9I TTAA 3 cut(s) 464, 669, 702
TscAI CASTG 4 cut(s) 52, 183, 301, 1216
TseFI GTSAC 2 cut(s) 1054, 1142
TseI GCWGC 3 cut(s) 32, 35, 689
Tsp45I GTSAC 2 cut(s) 1054, 1142
TspDTI ATGAA 8 cut(s) 359, 418, 646, 771, 953, 1081, 1473, 1484
TspRI CASTG 4 cut(s) 52, 183, 301, 1216
XapI RAATTY 3 cut(s) 509, 553, 1083
XbaI TCTAGA 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 1513
XmaJI CCTAGG 1 cut(s) 1028
XmiI GTMKAC 1 cut(s) 874
XspI CTAG 6 cut(s) 239, 329, 473, 500, 1029, 1220
ZraI GACGTC 1 cut(s) 1025
ZrmI AGTACT 1 cut(s) 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.