MD01G1206700.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000970
Physical Location & Seq
Reverse (-)
30198198 .. 30201930
3733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1206700.v1.1.491

Sequence Viewer

Length: 1500 bp
ATGGAGGAGCCGCTGCTCGACACAGCCGCCGGCGGCACCACCGAGTATAGCAATAAGCAGCTTATGTACGAAGGTGAGAGCGAAGACTATGCGCCGGTGAGGAGCTTTGGTGAGTTGCGGCAAATGTTTTGGATAGAGACGGTGAAGCTGTGGCAAATAGGCGGTTCGGCAGTTATCACTATCATGTGTATGTATGGGACTAACTCAGTCATCGTGCTCTTTGCCGGTCATCTCGGAACTATTGAGCTTTCCGCCATCTCCATTTCTCTTTCTGTCATTTCCACCTTCACTTATGGATTCATGCTTGGGATGGGGAGTGCTCTGGAAACCCTGTGTGGACAAGCATTTGGTGCTGGACAAGTTCACATGCTTGGTATCTACATGCAACGATCATGTATAATTCTATTCGTAACCTCCTTCTTACTGTTGCCGATTTACATCTTTGCCACACCGGTTCTAAAGTGGCTAGGCCAAGAAGACGACATAGCAAATGAAGCCGGAAAATTCACACTCCAAATCATCCCTCAATTGTTCTCACTTGCCATAAATTTCCCAGCTCAAAAGTTCCTCAAGGCTCAGAGGAAGGTGAAGGTGCTGGCATGGATTGCAGTGTTGGCCCTGCTCATACACATTGGAATGCTAGCGCTCTTCATATATGTGTTTGATTGGGGCACATCGGGGGCAGCTGTGTCATTTAACATCACAAGATGGGGGATTTCAATTGCTCAAGTTGTCTATATTATGGGTTGGTGTTATGAGGGTTGGACTGGATTTTCATGGCTGGCATTCAATGAGATTTGGGCATTTGTGAGACTTTCTCTTGCCTCTGCAGTCATGCTCTGCCTGGAAATTTGGTACATGATGAGTATACTCCTTCTCACAGGTGGTCTTTCTGATGCAGTTATAGCCGTTGATTCTCTTTCTATTTGCATGAACATCAACGGGTGGGAAGGAATGTTGTTTATTGGACTAAACGCCGCAATAAGTGTTCGCGTCTCCAATGAACTTGGGTTAAGACGTCCCAGAGCAGCCAAATACGCCGTTTATGTGACAGTATCTCAGTCTCTCTTAATTGGGCTTCTTTTCATGGTTATAATTTTAATAACCAAAGACTATTTTGCCGTAATTTTCACAAGTGACAAAGTGTTGCAACAAGCAGTCGCTAAGCTTGCATACCTCCTTGGTATAACTATGGTGCTCAATAGTGTTCAACCAGTAATATCAGGTGTTGCAATTGGTGGTGGATGGCAGGCAATGGTAGCTTATATAAACTTGGGTTGTTACTACATCTTTGGGCTTCCTCTTGGATACGTTCTTTGCCATGTTGCAAATTTAGGAGTCGCGGGACTTTGGGAGGGCATGATATGTGGAACTGCTCTCCAAACCATCATTCTGCTAATTATACTTTACAAAACAAACTGGAACAAAGAGGTGGAGCAAGCAACGAATCGTGTCCAGAAGTGGGGAGGGCAAGACATCAAAAATGCCAACGGAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

500

Amino Acids

54.85

Weight (kDa)

5.88

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 58 - 212 5.2e-29 MatE
MatE PF01554 276 - 438 5.2e-24 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1094
AatII GACGTC 1 cut(s) 1021
AccB1I GGYRCC 1 cut(s) 35
AccI GTMKAC 1 cut(s) 868
AccII CGCG 2 cut(s) 993, 1343
AciI CCGC 8 cut(s) 11, 27, 33, 118, 162, 252, 978, 1343
AcsI RAATTY 4 cut(s) 503, 547, 849, 1330
AcyI GRCGYC 1 cut(s) 1018
AfaI GTAC 2 cut(s) 68, 857
AfeI AGCGCT 1 cut(s) 645
AfiI CCNNNNNNNGG 1 cut(s) 1462
AgeI ACCGGT 1 cut(s) 451
AgsI TTSAA 3 cut(s) 720, 790, 1211
AjnI CCWGG 1 cut(s) 843
AjuI GAANNNNNNNTTGG 2 cut(s) 1374, 1406
AluBI AGCT 9 cut(s) 61, 105, 148, 247, 557, 686, 1168, 1262, 1496
AluI AGCT 9 cut(s) 61, 105, 148, 247, 557, 686, 1168, 1262, 1496
Alw21I GWGCWC 3 cut(s) 219, 322, 1200
Alw26I GTCTC 4 cut(s) 131, 805, 1000, 1068
Aor51HI AGCGCT 1 cut(s) 645
AoxI GGCC 2 cut(s) 469, 615
ApeKI GCWGC 4 cut(s) 13, 58, 683, 1028
ApoI RAATTY 4 cut(s) 503, 547, 849, 1330
AsiGI ACCGGT 1 cut(s) 451
AspLEI GCGC 2 cut(s) 94, 646
AspS9I GGNCC 1 cut(s) 616
AsuHPI GGTGA 5 cut(s) 86, 109, 122, 154, 598
AsuNHI GCTAGC 1 cut(s) 640
BaeGI GKGCMC 1 cut(s) 674
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 2 cut(s) 90, 483
Bbv12I GWGCWC 3 cut(s) 219, 322, 1200
BbvI GCAGC 3 cut(s) 70, 695, 1040
BccI CCATC 5 cut(s) 263, 304, 702, 1239, 1394
BceAI ACGGC 3 cut(s) 893, 1025, 1106
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 1 cut(s) 845
BciVI GTATCC 1 cut(s) 1301
BcoDI GTCTC 4 cut(s) 131, 805, 1000, 1068
BfaI CTAG 2 cut(s) 467, 641
BfmI CTRYAG 1 cut(s) 828
BfoI RGCGCY 1 cut(s) 647
BfuI GTATCC 1 cut(s) 1301
BisI GCNGC 9 cut(s) 11, 14, 27, 34, 59, 119, 684, 978, 1029
BlpI GCTNAGC 1 cut(s) 1164
BlsI GCNGC 9 cut(s) 12, 15, 28, 35, 60, 120, 685, 979, 1030
Bme1390I CCNGG 1 cut(s) 845
BmgT120I GGNCC 1 cut(s) 616
BmiI GGNNCC 2 cut(s) 9, 37
BmrFI CCNGG 1 cut(s) 845
BmsI GCATC 1 cut(s) 886
BmtI GCTAGC 1 cut(s) 644
BpiI GAAGAC 2 cut(s) 90, 483
BplI GAGNNNNNCTC 2 cut(s) 802, 834
Bpu1102I GCTNAGC 1 cut(s) 1164
BpuEI CTTGAG 2 cut(s) 554, 711
BsaBI GATNNNNATC 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 1018
BsaJI CCNNGG 1 cut(s) 1180
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bsc4I CCNNNNNNNGG 1 cut(s) 1462
Bse118I RCCGGY 4 cut(s) 29, 94, 224, 451
Bse1I ACTGG 3 cut(s) 772, 1214, 1424
Bse3DI GCAATG 1 cut(s) 1260
Bse8I GATNNNNATC 1 cut(s) 437
BseBI CCWGG 1 cut(s) 845
BseDI CCNNGG 1 cut(s) 1180
BseGI GGATG 3 cut(s) 315, 519, 1250
BseJI GATNNNNATC 1 cut(s) 437
BseLI CCNNNNNNNGG 1 cut(s) 1462
BseMI GCAATG 1 cut(s) 1260
BseMII CTCAG 3 cut(s) 219, 590, 1073
BseNI ACTGG 3 cut(s) 772, 1214, 1424
BseRI GAGGAG 2 cut(s) 20, 115
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 3 cut(s) 70, 695, 1040
BseYI CCCAGC 1 cut(s) 553
Bsh1236I CGCG 2 cut(s) 993, 1343
BshFI GGCC 2 cut(s) 471, 617
BshNI GGYRCC 1 cut(s) 35
BshTI ACCGGT 1 cut(s) 451
BsiHKAI GWGCWC 3 cut(s) 219, 322, 1200
BsiSI CCGG 5 cut(s) 30, 95, 225, 452, 498
BslFI GGGAC 3 cut(s) 211, 1005, 1359
BslI CCNNNNNNNGG 1 cut(s) 1462
BsmAI GTCTC 4 cut(s) 131, 805, 1000, 1068
BsmBI CGTCTC 2 cut(s) 131, 1000
BsmFI GGGAC 3 cut(s) 211, 1005, 1359
BsmI GAATGC 2 cut(s) 642, 785
BsnI GGCC 2 cut(s) 471, 617
Bsp1286I GDGCHC 4 cut(s) 219, 322, 674, 1200
Bsp143I GATC 1 cut(s) 389
Bsp1720I GCTNAGC 1 cut(s) 1164
BspACI CCGC 8 cut(s) 11, 27, 33, 118, 162, 252, 978, 1343
BspANI GGCC 2 cut(s) 471, 617
BspCNI CTCAG 3 cut(s) 218, 589, 1072
BspFNI CGCG 2 cut(s) 993, 1343
BspLI GGNNCC 2 cut(s) 9, 37
BspMAI CTGCAG 1 cut(s) 832
BspOI GCTAGC 1 cut(s) 644
BspQI GCTCTTC 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 1260
BsrFI RCCGGY 4 cut(s) 29, 94, 224, 451
BsrI ACTGG 3 cut(s) 772, 1214, 1424
BssAI RCCGGY 4 cut(s) 29, 94, 224, 451
BssECI CCNNGG 1 cut(s) 1180
BssMI GATC 1 cut(s) 389
BssNAI GTATAC 1 cut(s) 869
BssNI GRCGYC 1 cut(s) 1018
BssT1I CCWWGG 1 cut(s) 1180
Bst1107I GTATAC 1 cut(s) 869
Bst2UI CCWGG 1 cut(s) 845
Bst4CI ACNGT 3 cut(s) 142, 426, 1054
Bst6I CTCTTC 1 cut(s) 653
BstACI GRCGYC 1 cut(s) 1018
BstAPI GCANNNNNTGC 2 cut(s) 350, 605
BstC8I GCNNGC 7 cut(s) 31, 597, 642, 783, 1170, 1251, 1440
BstDEI CTNAG 4 cut(s) 205, 576, 1059, 1164
BstF5I GGATG 3 cut(s) 315, 519, 1250
BstFNI CGCG 2 cut(s) 993, 1343
BstH2I RGCGCY 1 cut(s) 647
BstHHI GCGC 2 cut(s) 94, 646
BstKTI GATC 1 cut(s) 392
BstMAI GTCTC 4 cut(s) 131, 805, 1000, 1068
BstMBI GATC 1 cut(s) 389
BstMWI GCNNNNNNNGC 9 cut(s) 350, 494, 605, 614, 905, 1037, 1169, 1259, 1493
BstNI CCWGG 1 cut(s) 845
BstNSI RCATGY 2 cut(s) 370, 385
BstSCI CCNGG 1 cut(s) 843
BstSFI CTRYAG 1 cut(s) 828
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 2 cut(s) 993, 1343
BstV1I GCAGC 3 cut(s) 70, 695, 1040
BstV2I GAAGAC 2 cut(s) 90, 483
BstZ17I GTATAC 1 cut(s) 869
BsuI GTATCC 1 cut(s) 1301
BsuRI GGCC 2 cut(s) 471, 617
BtsCI GGATG 3 cut(s) 315, 519, 1250
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 1 cut(s) 615
Cac8I GCNNGC 7 cut(s) 31, 597, 642, 783, 1170, 1251, 1440
CfoI GCGC 2 cut(s) 94, 646
Cfr10I RCCGGY 4 cut(s) 29, 94, 224, 451
Cfr13I GGNCC 1 cut(s) 616
CseI GACGC 1 cut(s) 982
Csp6I GTAC 2 cut(s) 67, 856
CspAI ACCGGT 1 cut(s) 451
CviQI GTAC 2 cut(s) 67, 856
DdeI CTNAG 4 cut(s) 205, 576, 1059, 1164
DpnI GATC 1 cut(s) 391
DpnII GATC 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EciI GGCGGA 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 1180
Eco47III AGCGCT 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 843
EcoT14I CCWWGG 1 cut(s) 1180
ErhI CCWWGG 1 cut(s) 1180
Esp3I CGTCTC 2 cut(s) 131, 1000
FaqI GGGAC 3 cut(s) 211, 1005, 1359
FauI CCCGC 1 cut(s) 1336
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 9 cut(s) 11, 14, 27, 34, 59, 119, 684, 978, 1029
FokI GGATG 3 cut(s) 322, 506, 1257
Fsp4HI GCNGC 9 cut(s) 11, 14, 27, 34, 59, 119, 684, 978, 1029
FspBI CTAG 2 cut(s) 467, 641
GlaI GCGC 2 cut(s) 93, 645
GluI GCNGC 9 cut(s) 11, 14, 27, 34, 59, 119, 684, 978, 1029
GsaI CCCAGC 1 cut(s) 557
HaeII RGCGCY 1 cut(s) 647
HaeIII GGCC 2 cut(s) 471, 617
HapII CCGG 5 cut(s) 30, 95, 225, 452, 498
HgaI GACGC 1 cut(s) 982
HhaI GCGC 2 cut(s) 94, 646
Hin1I GRCGYC 1 cut(s) 1018
Hin6I GCGC 2 cut(s) 92, 644
HinP1I GCGC 2 cut(s) 92, 644
HindIII AAGCTT 1 cut(s) 1166
HinfI GANTC 4 cut(s) 297, 914, 1338, 1447
HpaII CCGG 5 cut(s) 30, 95, 225, 452, 498
HphI GGTGA 5 cut(s) 86, 109, 122, 154, 598
Hpy166II GTNNAC 3 cut(s) 338, 364, 869
Hpy188I TCNGA 3 cut(s) 236, 579, 895
Hpy188III TCNNGA 2 cut(s) 323, 1456
Hpy8I GTNNAC 3 cut(s) 338, 364, 869
HpyAV CCTTC 7 cut(s) 65, 295, 427, 577, 583, 884, 944
HpyCH4III ACNGT 3 cut(s) 142, 426, 1054
HpyCH4IV ACGT 2 cut(s) 1018, 1311
HpyCH4V TGCA 9 cut(s) 385, 608, 830, 899, 930, 1150, 1172, 1232, 1328
HpyF10VI GCNNNNNNNGC 9 cut(s) 350, 494, 605, 614, 905, 1037, 1169, 1259, 1493
HpyF3I CTNAG 4 cut(s) 205, 576, 1059, 1164
HpySE526I ACGT 2 cut(s) 1018, 1311
Hsp92I GRCGYC 1 cut(s) 1018
HspAI GCGC 2 cut(s) 92, 644
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 389
LguI GCTCTTC 1 cut(s) 653
LmnI GCTCC 4 cut(s) 7, 102, 1435, 1493
Lsp1109I GCAGC 3 cut(s) 70, 695, 1040
LweI GCATC 1 cut(s) 886
MaeI CTAG 2 cut(s) 467, 641
MaeII ACGT 2 cut(s) 1018, 1311
MaeIII GTNAC 4 cut(s) 409, 1048, 1136, 1280
MalI GATC 1 cut(s) 391
MboI GATC 1 cut(s) 389
MboII GAAGA 3 cut(s) 95, 488, 640
MfeI CAATTG 3 cut(s) 527, 720, 1233
MhlI GDGCHC 4 cut(s) 219, 322, 674, 1200
MlyI GAGTC 1 cut(s) 1347
MmeI TCCRAC 1 cut(s) 743
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MseI TTAA 5 cut(s) 696, 1013, 1070, 1100, 1498
MslI CAYNNNNRTG 4 cut(s) 182, 188, 635, 656
MspA1I CMGCKG 2 cut(s) 13, 686
MspI CCGG 5 cut(s) 30, 95, 225, 452, 498
MspR9I CCNGG 1 cut(s) 845
MunI CAATTG 3 cut(s) 527, 720, 1233
Mva1269I GAATGC 2 cut(s) 642, 785
MvaI CCWGG 1 cut(s) 845
MvnI CGCG 2 cut(s) 993, 1343
MwoI GCNNNNNNNGC 9 cut(s) 350, 494, 605, 614, 905, 1037, 1169, 1259, 1493
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 1 cut(s) 389
NgoMIV GCCGGC 1 cut(s) 29
NheI GCTAGC 1 cut(s) 640
NlaIV GGNNCC 2 cut(s) 9, 37
NmuCI GTSAC 2 cut(s) 1048, 1136
NspI RCATGY 2 cut(s) 370, 385
PciSI GCTCTTC 1 cut(s) 653
PctI GAATGC 2 cut(s) 642, 785
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 3 cut(s) 297, 914, 1447
PinAI ACCGGT 1 cut(s) 451
PkrI GCNGC 9 cut(s) 12, 15, 28, 35, 60, 120, 685, 979, 1030
PleI GAGTC 1 cut(s) 1346
PpsI GAGTC 1 cut(s) 1346
PsiI TTATAA 1 cut(s) 1094
Psp6I CCWGG 1 cut(s) 843
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 1 cut(s) 843
PspN4I GGNNCC 2 cut(s) 9, 37
PspPI GGNCC 1 cut(s) 616
PstI CTGCAG 1 cut(s) 832
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 2 cut(s) 68, 857
RsaNI GTAC 2 cut(s) 67, 856
RseI CAYNNNNRTG 4 cut(s) 182, 188, 635, 656
SapI GCTCTTC 1 cut(s) 653
SaqAI TTAA 5 cut(s) 696, 1013, 1070, 1100, 1498
SatI GCNGC 9 cut(s) 11, 14, 27, 34, 59, 119, 684, 978, 1029
Sau3AI GATC 1 cut(s) 389
Sau96I GGNCC 1 cut(s) 616
SchI GAGTC 1 cut(s) 1347
ScrFI CCNGG 1 cut(s) 845
SduI GDGCHC 4 cut(s) 219, 322, 674, 1200
SfaNI GCATC 1 cut(s) 886
SfcI CTRYAG 1 cut(s) 828
SgrAI CRCCGGYG 2 cut(s) 29, 94
SmiMI CAYNNNNRTG 4 cut(s) 182, 188, 635, 656
SmlI CTYRAG 2 cut(s) 569, 726
SmoI CTYRAG 2 cut(s) 569, 726
SsiI CCGC 8 cut(s) 11, 27, 33, 118, 162, 252, 978, 1343
SspMI CTAG 2 cut(s) 467, 641
StyD4I CCNGG 1 cut(s) 843
StyI CCWWGG 1 cut(s) 1180
TaaI ACNGT 3 cut(s) 142, 426, 1054
TaiI ACGT 2 cut(s) 1021, 1314
TaqI TCGA 1 cut(s) 18
TauI GCSGC 5 cut(s) 13, 29, 36, 121, 980
TfiI GAWTC 3 cut(s) 297, 914, 1447
Tru1I TTAA 5 cut(s) 696, 1013, 1070, 1100, 1498
Tru9I TTAA 5 cut(s) 696, 1013, 1070, 1100, 1498
TscAI CASTG 1 cut(s) 615
TseFI GTSAC 2 cut(s) 1048, 1136
TseI GCWGC 4 cut(s) 13, 58, 683, 1028
Tsp45I GTSAC 2 cut(s) 1048, 1136
TspDTI ATGAA 7 cut(s) 289, 507, 640, 765, 947, 1017, 1075
TspRI CASTG 1 cut(s) 615
XapI RAATTY 4 cut(s) 503, 547, 849, 1330
XceI RCATGY 2 cut(s) 370, 385
XmiI GTMKAC 1 cut(s) 868
XspI CTAG 2 cut(s) 467, 641
ZraI GACGTC 1 cut(s) 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.