Rroxscaffold_4G00280850

Protein TRANSPARENT TESTA 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000970
Physical Location & Seq
Forward (+)
3315694 .. 3319034
3341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280850.1

Sequence Viewer

Length: 1131 bp
ATGGAGGCGCCGCTGCTGACCAACGGAGCTGTCACGGACTACGAGCCGGTGACAAGCTTTAAGCAGGCTCGGGAGGTGACGTGGAAAGAGACAGTGAAGCTATGGAGGATTGGTGGGCCTATAGCCTTCACCATAATCTGCAACTTCGGCACCAACTCCGTCTCCACCATGTTCGTCGGCCACATCGGAAACCTTGAGCTCTCCGCCGTCGCTATCTCTCTCTCCGTCATCAGTACCTTCTCTTTCGGCTTCATGCTTGGTATGGGAAGTGCACTAGAGACATTATGTGGTCAAGCATTTGGAGCTGGACGAGTTAACATGCTTGGCATCTACATGCAAAGGTCATGGATCATTCTATGGACAACCTGCCTTTTCCTTACTCCACTCTATTGCTTCTCTGCCCCAATCTTAAAGCTTCTTGGCCAAGATGACGATGTAGCGGACCTGGCGGGAACATTCACCGTCTTGTTTATATACTTGGTTGGTGTAAAGAAGGGTGGAGCGGGTTTTTCATGGTTGGCTTTCAAAGACATTTGGGCCTTTGTGAAGCTTTCGCTGGAATCAGCTGTCATGCTTTGCCTTGAGATTTGGTATATGATGAGTATCATCATTCTTACCGGCCATCTTGACAATGCGGTCATTGCTCTTGATTCACTTTCTATTTGCATGAATGTCAATGGATGGGAAGCAATGCTGTTCATTGGAATCAATGCTGCTATTAGTGTTCGAGTTTCTAATGAACTTGGATTGGGGCGTCCTAGAGCAGCTAAATACTCAGTCTATGTGACAGTGGCTCAATCTCTGCTGATTGGAATTTTTTGCATGGTTGTTATCTTGATTACCAAAGACTACTTCTCTGTCATCTTCACAAGCAACGCTGAATTGCAAGCAGCTGTTTCAAAGCTAGCATGGCTTCTCGGAATCACCATGGTTCTCAACAGTGTCCAGCCTGTGATTTCAGGTGTTGCTATCGGAGGGAATTTGGGGAGGCATGATATGTGGAACAGCACTTCAAACCTTGCTGCTCATTATAGTACTTTACAGAACCAACTGGAACAAGGAGGTGGAAGAAAGTTCAGACCGAATGAAGAAATGGGGAGGTCAAGATATAAAAGGCGAGACAATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

376

Amino Acids

41.15

Weight (kDa)

8.11

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 40 - 157 2.7e-24 MatE
MatE PF01554 189 - 324 3.8e-18 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 374
AccB1I GGYRCC 2 cut(s) 7, 149
AccBSI CCGCTC 1 cut(s) 503
AciI CCGC 6 cut(s) 11, 204, 440, 449, 503, 635
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 3 cut(s) 178, 421, 619
AcsI RAATTY 2 cut(s) 813, 979
AcyI GRCGYC 2 cut(s) 8, 754
AfaI GTAC 2 cut(s) 235, 1036
AgsI TTSAA 3 cut(s) 526, 900, 1014
AjiI CACGTC 1 cut(s) 81
AjnI CCWGG 1 cut(s) 444
AjuI GAANNNNNNNTTGG 2 cut(s) 836, 868
Alw21I GWGCWC 2 cut(s) 201, 274
Alw26I GTCTC 4 cut(s) 83, 166, 272, 1113
Alw44I GTGCAC 1 cut(s) 270
AlwI GGATC 1 cut(s) 356
Ama87I CYCGRG 1 cut(s) 69
AoxI GGCC 5 cut(s) 116, 178, 421, 537, 619
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 5 cut(s) 13, 713, 764, 890, 1022
ApoI RAATTY 2 cut(s) 813, 979
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 3 cut(s) 116, 442, 537
AsuHPI GGTGA 5 cut(s) 61, 88, 121, 451, 916
AsuNHI GCTAGC 1 cut(s) 904
AvaI CYCGRG 1 cut(s) 69
AvaII GGWCC 1 cut(s) 442
BaeGI GKGCMC 1 cut(s) 274
BalI TGGCCA 1 cut(s) 423
BanI GGYRCC 2 cut(s) 7, 149
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 201, 274
BbvI GCAGC 4 cut(s) 700, 776, 902, 1009
BccI CCATC 2 cut(s) 630, 675
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 1 cut(s) 446
BcoDI GTCTC 4 cut(s) 83, 166, 272, 1113
BfaI CTAG 3 cut(s) 275, 759, 905
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 374
BisI GCNGC 6 cut(s) 11, 14, 714, 765, 891, 1023
BlsI GCNGC 6 cut(s) 12, 15, 715, 766, 892, 1024
BmcAI AGTACT 1 cut(s) 1036
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 1 cut(s) 442
BmeT110I CYCGRG 1 cut(s) 69
BmgBI CACGTC 1 cut(s) 81
BmgT120I GGNCC 3 cut(s) 116, 442, 537
BmiI GGNNCC 2 cut(s) 9, 151
BmrFI CCNGG 1 cut(s) 446
BmsI GCATC 1 cut(s) 336
BmtI GCTAGC 1 cut(s) 908
BpuEI CTTGAG 2 cut(s) 215, 602
BsaBI GATNNNNATC 1 cut(s) 602
BsaHI GRCGYC 2 cut(s) 8, 754
BsaJI CCNNGG 1 cut(s) 927
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 97, 127, 146, 176
Bse118I RCCGGY 2 cut(s) 46, 617
Bse1I ACTGG 1 cut(s) 1056
Bse3DI GCAATG 2 cut(s) 639, 696
Bse8I GATNNNNATC 1 cut(s) 602
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 1 cut(s) 927
BseGI GGATG 1 cut(s) 686
BseJI GATNNNNATC 1 cut(s) 602
BseMI GCAATG 2 cut(s) 639, 696
BseMII CTCAG 1 cut(s) 789
BseNI ACTGG 1 cut(s) 1056
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 4 cut(s) 700, 776, 902, 1009
BshFI GGCC 5 cut(s) 118, 180, 423, 539, 621
BshNI GGYRCC 2 cut(s) 7, 149
BsiHKAI GWGCWC 2 cut(s) 201, 274
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 2 cut(s) 47, 618
BsmAI GTCTC 4 cut(s) 83, 166, 272, 1113
BsmBI CGTCTC 1 cut(s) 166
BsnI GGCC 5 cut(s) 118, 180, 423, 539, 621
BsoBI CYCGRG 1 cut(s) 69
Bsp1286I GDGCHC 2 cut(s) 201, 274
Bsp143I GATC 1 cut(s) 348
Bsp19I CCATGG 1 cut(s) 927
BspACI CCGC 6 cut(s) 11, 204, 440, 449, 503, 635
BspANI GGCC 5 cut(s) 118, 180, 423, 539, 621
BspCNI CTCAG 1 cut(s) 788
BspLI GGNNCC 2 cut(s) 9, 151
BspMI ACCTGC 1 cut(s) 374
BspOI GCTAGC 1 cut(s) 908
BspPI GGATC 1 cut(s) 356
BspT107I GGYRCC 2 cut(s) 7, 149
BsrBI CCGCTC 1 cut(s) 503
BsrDI GCAATG 2 cut(s) 639, 696
BsrFI RCCGGY 2 cut(s) 46, 617
BsrI ACTGG 1 cut(s) 1056
BssAI RCCGGY 2 cut(s) 46, 617
BssECI CCNNGG 1 cut(s) 927
BssMI GATC 1 cut(s) 348
BssNI GRCGYC 2 cut(s) 8, 754
BssT1I CCWWGG 1 cut(s) 927
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 4 cut(s) 94, 463, 790, 941
BstACI GRCGYC 2 cut(s) 8, 754
BstC8I GCNNGC 3 cut(s) 66, 888, 906
BstDEI CTNAG 1 cut(s) 775
BstDSI CCRYGG 1 cut(s) 927
BstF5I GGATG 1 cut(s) 686
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 1 cut(s) 351
BstMAI GTCTC 4 cut(s) 83, 166, 272, 1113
BstMBI GATC 1 cut(s) 348
BstMWI GCNNNNNNNGC 5 cut(s) 147, 302, 446, 641, 910
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 2 cut(s) 322, 337
BstSCI CCNGG 1 cut(s) 444
BstSFI CTRYAG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 4 cut(s) 700, 776, 902, 1009
BsuRI GGCC 5 cut(s) 118, 180, 423, 539, 621
BtgI CCRYGG 1 cut(s) 927
BtrI CACGTC 1 cut(s) 81
BtsCI GGATG 1 cut(s) 686
BtsIMutI CAGTG 3 cut(s) 99, 795, 946
BveI ACCTGC 1 cut(s) 374
Cac8I GCNNGC 3 cut(s) 66, 888, 906
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 2 cut(s) 46, 617
Cfr13I GGNCC 3 cut(s) 116, 442, 537
CseI GACGC 1 cut(s) 743
Csp6I GTAC 2 cut(s) 234, 1035
CviQI GTAC 2 cut(s) 234, 1035
DdeI CTNAG 1 cut(s) 775
DinI GGCGCC 1 cut(s) 9
DpnI GATC 1 cut(s) 350
DpnII GATC 1 cut(s) 348
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
EaeI YGGCCR 3 cut(s) 178, 421, 619
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 927
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 442
Eco53kI GAGCTC 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 927
EcoT38I GRGCYC 1 cut(s) 201
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 927
Esp3I CGTCTC 1 cut(s) 166
FauI CCCGC 2 cut(s) 442, 496
Fnu4HI GCNGC 6 cut(s) 11, 14, 714, 765, 891, 1023
FokI GGATG 1 cut(s) 693
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 6 cut(s) 11, 14, 714, 765, 891, 1023
FspBI CTAG 3 cut(s) 275, 759, 905
GlaI GCGC 1 cut(s) 9
GluI GCNGC 6 cut(s) 11, 14, 714, 765, 891, 1023
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 5 cut(s) 118, 180, 423, 539, 621
HapII CCGG 2 cut(s) 47, 618
HgaI GACGC 1 cut(s) 743
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 2 cut(s) 8, 754
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 3 cut(s) 55, 413, 548
HinfI GANTC 4 cut(s) 560, 650, 705, 921
HpaI GTTAAC 1 cut(s) 316
HpaII CCGG 2 cut(s) 47, 618
HphI GGTGA 5 cut(s) 61, 88, 121, 451, 916
Hpy166II GTNNAC 2 cut(s) 272, 316
Hpy188I TCNGA 4 cut(s) 188, 920, 974, 1079
Hpy188III TCNNGA 5 cut(s) 71, 626, 647, 835, 1104
Hpy8I GTNNAC 2 cut(s) 272, 316
Hpy99I CGWCG 2 cut(s) 179, 212
HpyAV CCTTC 3 cut(s) 136, 247, 487
HpyCH4III ACNGT 4 cut(s) 94, 463, 790, 941
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 6 cut(s) 141, 272, 337, 666, 822, 886
HpyF10VI GCNNNNNNNGC 5 cut(s) 147, 302, 446, 641, 910
HpyF3I CTNAG 1 cut(s) 775
HpySE526I ACGT 1 cut(s) 80
Hsp92I GRCGYC 2 cut(s) 8, 754
HspAI GCGC 1 cut(s) 8
KasI GGCGCC 1 cut(s) 7
KspAI GTTAAC 1 cut(s) 316
Kzo9I GATC 1 cut(s) 348
LmnI GCTCC 3 cut(s) 26, 302, 500
Lsp1109I GCAGC 4 cut(s) 700, 776, 902, 1009
LweI GCATC 1 cut(s) 336
MaeI CTAG 3 cut(s) 275, 759, 905
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 4 cut(s) 31, 49, 76, 784
MalI GATC 1 cut(s) 350
MbiI CCGCTC 1 cut(s) 503
MboI GATC 1 cut(s) 348
MboII GAAGA 3 cut(s) 856, 1080, 1100
MhlI GDGCHC 2 cut(s) 201, 274
MlsI TGGCCA 1 cut(s) 423
MluCI AATT 3 cut(s) 813, 881, 979
MluNI TGGCCA 1 cut(s) 423
Mly113I GGCGCC 1 cut(s) 8
MnlI CCTC 6 cut(s) 67, 99, 968, 981, 1055, 1092
Mox20I TGGCCA 1 cut(s) 423
MscI TGGCCA 1 cut(s) 423
MseI TTAA 3 cut(s) 60, 315, 410
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 423
MspA1I CMGCKG 3 cut(s) 13, 566, 893
MspI CCGG 2 cut(s) 47, 618
MspR9I CCNGG 1 cut(s) 446
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 5 cut(s) 147, 302, 446, 641, 910
NarI GGCGCC 1 cut(s) 8
NcoI CCATGG 1 cut(s) 927
NdeII GATC 1 cut(s) 348
NheI GCTAGC 1 cut(s) 904
NlaIV GGNNCC 2 cut(s) 9, 151
NmuCI GTSAC 4 cut(s) 31, 49, 76, 784
NspI RCATGY 2 cut(s) 322, 337
PfeI GAWTC 4 cut(s) 560, 650, 705, 921
PkrI GCNGC 6 cut(s) 12, 15, 715, 766, 892, 1024
PluTI GGCGCC 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 444
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 2 cut(s) 9, 151
PspPI GGNCC 3 cut(s) 116, 442, 537
PvuII CAGCTG 2 cut(s) 566, 893
RsaI GTAC 2 cut(s) 235, 1036
RsaNI GTAC 2 cut(s) 234, 1035
RseI CAYNNNNRTG 1 cut(s) 332
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 3 cut(s) 60, 315, 410
SatI GCNGC 6 cut(s) 11, 14, 714, 765, 891, 1023
Sau3AI GATC 1 cut(s) 348
Sau96I GGNCC 3 cut(s) 116, 442, 537
ScaI AGTACT 1 cut(s) 1036
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 2 cut(s) 201, 274
SfaNI GCATC 1 cut(s) 336
SfcI CTRYAG 1 cut(s) 120
SfoI GGCGCC 1 cut(s) 9
SinI GGWCC 1 cut(s) 442
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 2 cut(s) 194, 581
SmoI CTYRAG 2 cut(s) 194, 581
Sse9I AATT 3 cut(s) 813, 881, 979
SsiI CCGC 6 cut(s) 11, 204, 440, 449, 503, 635
SspDI GGCGCC 1 cut(s) 7
SspMI CTAG 3 cut(s) 275, 759, 905
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 4 cut(s) 94, 463, 790, 941
TaiI ACGT 1 cut(s) 83
TaqI TCGA 1 cut(s) 727
TaqII GACCGA 1 cut(s) 1096
TasI AATT 3 cut(s) 813, 881, 979
TatI WGTACW 1 cut(s) 1034
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 4 cut(s) 560, 650, 705, 921
Tru1I TTAA 3 cut(s) 60, 315, 410
Tru9I TTAA 3 cut(s) 60, 315, 410
TscAI CASTG 3 cut(s) 99, 795, 946
TseFI GTSAC 4 cut(s) 31, 49, 76, 784
TseI GCWGC 5 cut(s) 13, 713, 764, 890, 1022
Tsp45I GTSAC 4 cut(s) 31, 49, 76, 784
TspDTI ATGAA 6 cut(s) 241, 501, 683, 688, 753, 1101
TspGWI ACGGA 4 cut(s) 39, 50, 148, 214
TspRI CASTG 3 cut(s) 99, 795, 946
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 442
XapI RAATTY 2 cut(s) 813, 979
XceI RCATGY 2 cut(s) 322, 337
XspI CTAG 3 cut(s) 275, 759, 905
ZrmI AGTACT 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.