MD01G1129300.v1.1
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001841
Physical Location & Seq
Forward (+)
24037191 .. 24037877
687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1129300.v1.1.491

Sequence Viewer

Length: 381 bp
ATGGCAGGAAAAGAAGGCCACGCAATCGGGATTGATCTGGGGACAACGTACTCGTGTGTCGCAGTGTGGGAGCACGAACGTGTGGAAATCATAGTGAATGATCAGGGTAACAGGACCACTCCATCTTGTGTTGCCTTCACTGAGACTGAGCGATTTGTTGGTGATTCAGCCATCAACCAAATCATCAGGAACCCCACCAATTCCATTTTTGATGCAAAGAGGTTAATTGGTAGGAGATTCAGTGATGCCACGGTTCAAAGTGATTTGAAGCTCTGGCCATTTAAAGTCACTGAAGGTCCTGGCGGCAAGCCCTTGATTGTGGTTACCCACGAGGGACAAGAGAAACAGTTCGCTGCCGAAGAAATTGTTACAATTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

127

Amino Acids

13.96

Weight (kDa)

5.34

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 8 - 123 1e-42 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 303
AcoI YGGCCR 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 2 cut(s) 257, 268
AjnI CCWGG 1 cut(s) 298
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 1 cut(s) 271
AluI AGCT 1 cut(s) 271
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 137
AoxI GGCC 2 cut(s) 16, 275
ApeKI GCWGC 1 cut(s) 353
Asp700I GAANNNNTTC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 114, 296
AsuHPI GGTGA 1 cut(s) 173
AvaII GGWCC 2 cut(s) 114, 296
BalI TGGCCA 1 cut(s) 277
BauI CACGAG 2 cut(s) 52, 329
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 340
BccI CCATC 2 cut(s) 130, 179
BciT130I CCWGG 1 cut(s) 300
BclI TGATCA 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 137
BisI GCNGC 2 cut(s) 304, 354
BlsI GCNGC 2 cut(s) 305, 355
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 2 cut(s) 114, 296
BmgT120I GGNCC 2 cut(s) 114, 296
BmiI GGNNCC 1 cut(s) 191
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 2 cut(s) 202, 235
BsaJI CCNNGG 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 249
BseMII CTCAG 2 cut(s) 132, 138
BseXI GCAGC 1 cut(s) 340
BshFI GGCC 2 cut(s) 18, 277
BsiHKAI GWGCWC 1 cut(s) 75
BslFI GGGAC 2 cut(s) 55, 348
BsmAI GTCTC 1 cut(s) 137
BsmFI GGGAC 2 cut(s) 55, 348
BsnI GGCC 2 cut(s) 18, 277
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 34, 100
BspACI CCGC 1 cut(s) 303
BspANI GGCC 2 cut(s) 18, 277
BspCNI CTCAG 2 cut(s) 133, 139
BspLI GGNNCC 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 34, 100
BssSI CACGAG 2 cut(s) 52, 329
Bst2BI CACGAG 2 cut(s) 52, 329
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 253, 348
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 2 cut(s) 141, 147
BstDSI CCRYGG 1 cut(s) 249
BstEII GGTNACC 1 cut(s) 322
BstKTI GATC 2 cut(s) 37, 103
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 2 cut(s) 34, 100
BstNI CCWGG 1 cut(s) 300
BstPI GGTNACC 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 298
BstV1I GCAGC 1 cut(s) 340
BsuRI GGCC 2 cut(s) 18, 277
BtgI CCRYGG 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 4 cut(s) 69, 138, 247, 288
Cac8I GCNNGC 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 114, 296
Csp6I GTAC 1 cut(s) 49
CviJI RGCY 5 cut(s) 18, 170, 271, 277, 310
CviKI_1 RGCY 5 cut(s) 18, 170, 271, 277, 310
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 2 cut(s) 141, 147
DpnI GATC 2 cut(s) 36, 102
DpnII GATC 2 cut(s) 34, 100
DraI TTTAAA 1 cut(s) 283
EaeI YGGCCR 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 114, 296
Eco57I CTGAAG 1 cut(s) 312
Eco91I GGTNACC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 296
EcoO65I GGTNACC 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 298
FaiI YATR 1 cut(s) 92
FaqI GGGAC 2 cut(s) 55, 348
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 2 cut(s) 304, 354
Fsp4HI GCNGC 2 cut(s) 304, 354
GluI GCNGC 2 cut(s) 304, 354
HaeIII GGCC 2 cut(s) 18, 277
HinfI GANTC 2 cut(s) 164, 237
HphI GGTGA 1 cut(s) 173
Hpy188III TCNNGA 2 cut(s) 28, 187
HpyAV CCTTC 3 cut(s) 8, 145, 287
HpyCH4III ACNGT 2 cut(s) 253, 348
HpyCH4IV ACGT 2 cut(s) 47, 79
HpyCH4V TGCA 1 cut(s) 215
HpyF3I CTNAG 2 cut(s) 141, 147
HpySE526I ACGT 2 cut(s) 47, 79
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 2 cut(s) 34, 100
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 7 cut(s) 23, 89, 97, 172, 259, 285, 312
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 2 cut(s) 202, 235
MaeII ACGT 2 cut(s) 47, 79
MaeIII GTNAC 4 cut(s) 107, 286, 322, 367
MalI GATC 2 cut(s) 36, 102
MboI GATC 2 cut(s) 34, 100
MboII GAAGA 1 cut(s) 371
MhlI GDGCHC 1 cut(s) 75
MlsI TGGCCA 1 cut(s) 277
MluCI AATT 4 cut(s) 199, 225, 363, 372
MluNI TGGCCA 1 cut(s) 277
MnlI CCTC 2 cut(s) 213, 325
Mox20I TGGCCA 1 cut(s) 277
MroXI GAANNNNTTC 1 cut(s) 347
MscI TGGCCA 1 cut(s) 277
MseI TTAA 2 cut(s) 224, 282
MslI CAYNNNNRTG 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 277
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
NdeII GATC 2 cut(s) 34, 100
NlaIV GGNNCC 1 cut(s) 191
NmuCI GTSAC 1 cut(s) 286
OliI CACNNNNGTG 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 2 cut(s) 164, 237
PkrI GCNGC 2 cut(s) 305, 355
PpuMI RGGWCCY 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 298
PspEI GGTNACC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 1 cut(s) 191
PspPI GGNCC 2 cut(s) 114, 296
PspPPI RGGWCCY 1 cut(s) 296
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 78
SaqAI TTAA 2 cut(s) 224, 282
SatI GCNGC 2 cut(s) 304, 354
Sau3AI GATC 2 cut(s) 34, 100
Sau96I GGNCC 2 cut(s) 114, 296
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 1 cut(s) 75
SetI ASST 5 cut(s) 50, 82, 224, 273, 298
SfaNI GCATC 2 cut(s) 202, 235
SinI GGWCC 2 cut(s) 114, 296
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 4 cut(s) 199, 225, 363, 372
SsiI CCGC 1 cut(s) 303
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 253, 348
TaiI ACGT 2 cut(s) 50, 82
TasI AATT 4 cut(s) 199, 225, 363, 372
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 2 cut(s) 164, 237
Tru1I TTAA 2 cut(s) 224, 282
Tru9I TTAA 2 cut(s) 224, 282
TscAI CASTG 4 cut(s) 69, 145, 247, 295
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 286
TspRI CASTG 4 cut(s) 69, 145, 247, 295
VpaK11BI GGWCC 2 cut(s) 114, 296
XmnI GAANNNNTTC 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.