pycom04g11280
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001841
Physical Location & Seq
Forward (+)
14172079 .. 14173024
946 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11280.1

Sequence Viewer

Length: 219 bp
ATGGCCGGTAAAGGAGAAGGTCCGGCCATCGGAATCGATCTCGGCACCACATACTCATGCGTCGGTGTTTGGCAACACGACCGTGTAGAGATCATCGCCAACGACCAGGGTAACAGAACCACCCCGTCGTACGTTGCTTTCACTGATACCGAGCGGTTGATCGGAGATGCCGCCAAGAATCAGGTCGCCATGAACCCCATCAACACCGTCTTCGGCTAA

Protein Analysis

73

Amino Acids

7.65

Weight (kDa)

4.7

Isoelectric Point (pI)

10.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 71 3.4e-28 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 44
AccBSI CCGCTC 1 cut(s) 154
AciI CCGC 2 cut(s) 154, 171
AcoI YGGCCR 2 cut(s) 3, 24
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 29
AjnI CCWGG 1 cut(s) 105
AleI CACNNNNGTG 1 cut(s) 81
AoxI GGCC 2 cut(s) 3, 24
AspS9I GGNCC 1 cut(s) 20
AvaII GGWCC 1 cut(s) 20
BanI GGYRCC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 202
BccI CCATC 2 cut(s) 35, 206
BciT130I CCWGG 1 cut(s) 107
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 202
Bsa29I ATCGAT 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 106
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 5
BseBI CCWGG 1 cut(s) 107
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 2 cut(s) 5, 26
BshNI GGYRCC 1 cut(s) 44
BshVI ATCGAT 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 82
BsiSI CCGG 2 cut(s) 6, 23
BsiWI CGTACG 1 cut(s) 129
BslI CCNNNNNNNGG 1 cut(s) 29
BsnI GGCC 2 cut(s) 5, 26
Bsp143I GATC 3 cut(s) 37, 90, 159
BspACI CCGC 2 cut(s) 154, 171
BspANI GGCC 2 cut(s) 5, 26
BspDI ATCGAT 1 cut(s) 36
BspLI GGNNCC 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 5
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 3 cut(s) 37, 90, 159
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 2 cut(s) 83, 208
BstKTI GATC 3 cut(s) 40, 93, 162
BstMBI GATC 3 cut(s) 37, 90, 159
BstMCI CGRYCG 1 cut(s) 82
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 202
Bsu15I ATCGAT 1 cut(s) 36
BsuRI GGCC 2 cut(s) 5, 26
BsuTUI ATCGAT 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 130
CviAII CATG 2 cut(s) 57, 190
CviJI RGCY 3 cut(s) 5, 26, 216
CviKI_1 RGCY 3 cut(s) 5, 26, 216
CviQI GTAC 1 cut(s) 130
DpnI GATC 3 cut(s) 39, 92, 161
DpnII GATC 3 cut(s) 37, 90, 159
EaeI YGGCCR 2 cut(s) 3, 24
Eco47I GGWCC 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 105
FaeI CATG 2 cut(s) 60, 193
FaiI YATR 3 cut(s) 52, 58, 191
FatI CATG 2 cut(s) 56, 189
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
GluI GCNGC 1 cut(s) 171
HaeIII GGCC 2 cut(s) 5, 26
HapII CCGG 2 cut(s) 6, 23
HgaI GACGC 1 cut(s) 49
Hin1II CATG 2 cut(s) 60, 193
HinfI GANTC 2 cut(s) 33, 178
HpaII CCGG 2 cut(s) 6, 23
Hpy188I TCNGA 2 cut(s) 32, 164
Hpy99I CGWCG 2 cut(s) 65, 130
HpyAV CCTTC 1 cut(s) 11
HpyCH4III ACNGT 2 cut(s) 83, 208
HpyCH4IV ACGT 1 cut(s) 132
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 2 cut(s) 60, 193
Kzo9I GATC 3 cut(s) 37, 90, 159
LpnPI CCDG 5 cut(s) 19, 36, 92, 119, 167
LweI GCATC 1 cut(s) 157
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 110
MalI GATC 3 cut(s) 39, 92, 161
MbiI CCGCTC 1 cut(s) 154
MboI GATC 3 cut(s) 37, 90, 159
MboII GAAGA 1 cut(s) 202
MslI CAYNNNNRTG 2 cut(s) 55, 81
MspI CCGG 2 cut(s) 6, 23
MspR9I CCNGG 1 cut(s) 107
MvaI CCWGG 1 cut(s) 107
NdeII GATC 3 cut(s) 37, 90, 159
NlaIII CATG 2 cut(s) 60, 193
NlaIV GGNNCC 1 cut(s) 46
NmeAIII GCCGAG 1 cut(s) 21
OliI CACNNNNGTG 1 cut(s) 81
PfeI GAWTC 2 cut(s) 33, 178
Pfl23II CGTACG 1 cut(s) 129
PkrI GCNGC 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspLI CGTACG 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 1 cut(s) 20
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
RseI CAYNNNNRTG 2 cut(s) 55, 81
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 3 cut(s) 37, 90, 159
Sau96I GGNCC 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 107
SetI ASST 3 cut(s) 22, 135, 186
SfaNI GCATC 1 cut(s) 157
SinI GGWCC 1 cut(s) 20
SmiMI CAYNNNNRTG 2 cut(s) 55, 81
SsiI CCGC 2 cut(s) 154, 171
StyD4I CCNGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 83, 208
TaiI ACGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 36
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 2 cut(s) 33, 178
TscAI CASTG 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 206
TspRI CASTG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.