Rh3BG070700
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001841
Physical Location & Seq
Reverse (-)
4737164 .. 4737555
392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG070700.1

Sequence Viewer

Length: 294 bp
ATGGAGGTGAAGAATACCAAAGCGGTGTCATCGATATTTCTGCTCGTCTTTGTTTCAGACTTCTTGTGTGGGATGGCATTAGCTGCAAAGGGTTCATCTAATCCTTGGCCAGTGATCGGAATCAGCCTCGGAACCACCTATTCATGTGTGGGTGTGTACATAAATGGCCATGGCAATAGAATCACACCCTCTTGGGTTGCATTCACTGACACCGAGCTTCTAGTGGGAGATGCAGAAAGAATCAAGCGCATATTAACCCAGAACGTACCCTTTTTGATATCCAGCGGCTCATAG

Protein Analysis

97

Amino Acids

10.38

Weight (kDa)

7.8

Isoelectric Point (pI)

19.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 38 - 84 7.5e-09 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 23, 285
AcoI YGGCCR 2 cut(s) 107, 166
AfaI GTAC 2 cut(s) 158, 267
AfiI CCNNNNNNNGG 1 cut(s) 116
AluBI AGCT 2 cut(s) 83, 217
AluI AGCT 2 cut(s) 83, 217
AoxI GGCC 2 cut(s) 107, 166
ApeKI GCWGC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 249
AsuHPI GGTGA 1 cut(s) 19
BalI TGGCCA 2 cut(s) 109, 168
BbvI GCAGC 1 cut(s) 70
BccI CCATC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BfaI CTAG 1 cut(s) 221
BisI GCNGC 2 cut(s) 84, 286
BlsI GCNGC 2 cut(s) 85, 287
BmiI GGNNCC 1 cut(s) 133
BmsI GCATC 1 cut(s) 220
Bsa29I ATCGAT 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 119
BsaJI CCNNGG 3 cut(s) 104, 127, 169
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 110
Bse8I GATNNNNATC 1 cut(s) 119
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 104, 127, 169
BseGI GGATG 1 cut(s) 78
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 110
BseXI GCAGC 1 cut(s) 70
BshFI GGCC 2 cut(s) 109, 168
BshVI ATCGAT 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 116
BsmI GAATGC 1 cut(s) 200
BsnI GGCC 2 cut(s) 109, 168
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 1 cut(s) 114
Bsp19I CCATGG 1 cut(s) 169
BspACI CCGC 2 cut(s) 23, 285
BspANI GGCC 2 cut(s) 109, 168
BspDI ATCGAT 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 133
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 3 cut(s) 104, 127, 169
BssMI GATC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 104, 169
BstAPI GCANNNNNTGC 1 cut(s) 83
BstAUI TGTACA 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 70
Bsu15I ATCGAT 1 cut(s) 32
BsuRI GGCC 2 cut(s) 109, 168
BsuTUI ATCGAT 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 78
BtsIMutI CAGTG 2 cut(s) 117, 204
CfoI GCGC 1 cut(s) 249
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 2 cut(s) 157, 266
CviAII CATG 2 cut(s) 144, 170
CviJI RGCY 6 cut(s) 83, 109, 126, 168, 217, 288
CviKI_1 RGCY 6 cut(s) 83, 109, 126, 168, 217, 288
CviQI GTAC 2 cut(s) 157, 266
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
EaeI YGGCCR 2 cut(s) 107, 166
Eco130I CCWWGG 2 cut(s) 104, 169
Eco32I GATATC 1 cut(s) 279
EcoRV GATATC 1 cut(s) 279
EcoT14I CCWWGG 2 cut(s) 104, 169
ErhI CCWWGG 2 cut(s) 104, 169
FaeI CATG 2 cut(s) 147, 173
FaiI YATR 5 cut(s) 145, 161, 171, 251, 292
FatI CATG 2 cut(s) 143, 169
Fnu4HI GCNGC 2 cut(s) 84, 286
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 2 cut(s) 84, 286
FspBI CTAG 1 cut(s) 221
GlaI GCGC 1 cut(s) 248
GluI GCNGC 2 cut(s) 84, 286
HaeIII GGCC 2 cut(s) 109, 168
HhaI GCGC 1 cut(s) 249
Hin1II CATG 2 cut(s) 147, 173
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HinfI GANTC 3 cut(s) 120, 180, 240
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 3 cut(s) 58, 119, 131
Hpy8I GTNNAC 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 3 cut(s) 86, 200, 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 147, 173
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 1 cut(s) 114
LpnPI CCDG 2 cut(s) 123, 272
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 220
MaeI CTAG 1 cut(s) 221
MaeII ACGT 1 cut(s) 264
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 1 cut(s) 22
MlsI TGGCCA 2 cut(s) 109, 168
MluNI TGGCCA 2 cut(s) 109, 168
MnlI CCTC 2 cut(s) 137, 199
Mox20I TGGCCA 2 cut(s) 109, 168
MscI TGGCCA 2 cut(s) 109, 168
MseI TTAA 1 cut(s) 254
Msp20I TGGCCA 2 cut(s) 109, 168
MspA1I CMGCKG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 169
NdeII GATC 1 cut(s) 114
NlaIII CATG 2 cut(s) 147, 173
NlaIV GGNNCC 1 cut(s) 133
PctI GAATGC 1 cut(s) 200
PfeI GAWTC 3 cut(s) 120, 180, 240
PkrI GCNGC 2 cut(s) 85, 287
PspN4I GGNNCC 1 cut(s) 133
RsaI GTAC 2 cut(s) 158, 267
RsaNI GTAC 2 cut(s) 157, 266
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 2 cut(s) 84, 286
Sau3AI GATC 1 cut(s) 114
SetI ASST 5 cut(s) 9, 85, 140, 219, 267
SfaNI GCATC 1 cut(s) 220
SsiI CCGC 2 cut(s) 23, 285
SspMI CTAG 1 cut(s) 221
StyI CCWWGG 2 cut(s) 104, 169
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 32
TatI WGTACW 1 cut(s) 156
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 3 cut(s) 120, 180, 240
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TscAI CASTG 2 cut(s) 117, 211
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 2 cut(s) 84, 132
TspRI CASTG 2 cut(s) 117, 211
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.