MD01G1129600.v1.1
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001841
Physical Location & Seq
Forward (+)
24048143 .. 24049160
1018 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1129600.v1.1.491

Sequence Viewer

Length: 546 bp
ATGATAGACGAAGCTCTGAACTCTCTCTTCCCCACTAAACTAGCCAGACTCACTCTTCCCAGTCCCTCACCATTACTCTCACTGAAATCAACTTTCTTCTCTTCTCTTCTTCTTTATCGTGTTCCCGTGCAGAGAATGGCAGGAGAAGAAGGCCACGCAATCGGGATTGATCTGGGGACAACGTACTCTTGTGTCGCAGTGTGGGAGCACGACCGTGTGGAAATCATAGTGAACGATCAGGGTAACAGGACCACTCCATCTTGTGTTGCCTTCACTGAGACTGAGCGATTGGTTGGTGATTCGGCCATCAATCAAATCATCAGGAACCCCACCAACTCCATTTTTGATGCGAAGAGGTTAATTGGTAGGAGATTCAATGACGCCACTGTTCAAAGTGATTTGAAGCTCTGGCCATTTAGAGTCACTGAGGGTCCTGGCGGCAAGCCCTTGATTGTGGTCACCCACGAGGGACAAGAAAAACAGTTTGCTGCCGAAGAAATTTCAGCCATGGTTCTAGTGAAAATGCGGGAGATTGCTGAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

182

Amino Acids

20.04

Weight (kDa)

5.66

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 53 - 181 1.2e-48 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 438, 526
AcoI YGGCCR 2 cut(s) 303, 410
AcsI RAATTY 1 cut(s) 498
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 1 cut(s) 185
AgsI TTSAA 3 cut(s) 376, 392, 403
AjnI CCWGG 1 cut(s) 433
AleI CACNNNNGTG 1 cut(s) 213
AluBI AGCT 2 cut(s) 14, 406
AluI AGCT 2 cut(s) 14, 406
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 272
AoxI GGCC 4 cut(s) 151, 303, 410, 540
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 1 cut(s) 498
AspS9I GGNCC 2 cut(s) 249, 431
AsuHPI GGTGA 3 cut(s) 60, 308, 451
AvaII GGWCC 2 cut(s) 249, 431
BalI TGGCCA 1 cut(s) 412
BauI CACGAG 1 cut(s) 464
Bbv12I GWGCWC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 1 cut(s) 475
BccI CCATC 2 cut(s) 265, 314
BciT130I CCWGG 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 3 cut(s) 41, 515, 544
BisI GCNGC 2 cut(s) 439, 489
BlsI GCNGC 2 cut(s) 440, 490
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 2 cut(s) 249, 431
BmgT120I GGNCC 2 cut(s) 249, 431
BmiI GGNNCC 2 cut(s) 326, 432
BmrFI CCNGG 1 cut(s) 435
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 54
Bpu10I CCTNAGC 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 507
Bse1I ACTGG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 507
BseMII CTCAG 4 cut(s) 267, 273, 417, 528
BseNI ACTGG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 475
BsgI GTGCAG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 4 cut(s) 153, 305, 412, 542
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 210
BslFI GGGAC 3 cut(s) 48, 190, 483
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 3 cut(s) 48, 190, 483
BsnI GGCC 4 cut(s) 153, 305, 412, 542
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 169, 235
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 2 cut(s) 438, 526
BspANI GGCC 4 cut(s) 153, 305, 412, 542
BspCNI CTCAG 4 cut(s) 268, 274, 418, 529
BspLI GGNNCC 2 cut(s) 326, 432
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 2 cut(s) 169, 235
BssNI GRCGYC 1 cut(s) 381
BssSI CACGAG 1 cut(s) 464
BssT1I CCWWGG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 464
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 3 cut(s) 215, 388, 483
Bst6I CTCTTC 5 cut(s) 32, 60, 106, 111, 347
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 443
BstDEI CTNAG 4 cut(s) 276, 282, 426, 537
BstDSI CCRYGG 1 cut(s) 507
BstEII GGTNACC 1 cut(s) 457
BstKTI GATC 2 cut(s) 172, 238
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 2 cut(s) 169, 235
BstMCI CGRYCG 1 cut(s) 214
BstNI CCWGG 1 cut(s) 435
BstPI GGTNACC 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 433
BstV1I GCAGC 1 cut(s) 475
BsuRI GGCC 4 cut(s) 153, 305, 412, 542
BtgI CCRYGG 1 cut(s) 507
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 5 cut(s) 80, 204, 273, 384, 423
Cac8I GCNNGC 1 cut(s) 443
Cfr13I GGNCC 2 cut(s) 249, 431
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 184
CviAII CATG 1 cut(s) 508
CviJI RGCY 9 cut(s) 14, 44, 153, 305, 406, 412, 445, 506, 542
CviKI_1 RGCY 9 cut(s) 14, 44, 153, 305, 406, 412, 445, 506, 542
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 4 cut(s) 276, 282, 426, 537
DpnI GATC 2 cut(s) 171, 237
DpnII GATC 2 cut(s) 169, 235
EaeI YGGCCR 2 cut(s) 303, 410
Eam1104I CTCTTC 5 cut(s) 32, 60, 106, 111, 347
EarI CTCTTC 5 cut(s) 32, 60, 106, 111, 347
Eco130I CCWWGG 1 cut(s) 507
Eco147I AGGCCT 1 cut(s) 542
Eco47I GGWCC 2 cut(s) 249, 431
Eco91I GGTNACC 1 cut(s) 457
EcoO109I RGGNCCY 1 cut(s) 431
EcoO65I GGTNACC 1 cut(s) 457
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 1 cut(s) 511
FaiI YATR 2 cut(s) 227, 509
FaqI GGGAC 3 cut(s) 48, 190, 483
FatI CATG 1 cut(s) 507
FauI CCCGC 1 cut(s) 519
Fnu4HI GCNGC 2 cut(s) 439, 489
Fsp4HI GCNGC 2 cut(s) 439, 489
FspBI CTAG 3 cut(s) 41, 515, 544
GluI GCNGC 2 cut(s) 439, 489
HaeIII GGCC 4 cut(s) 153, 305, 412, 542
HgaI GACGC 1 cut(s) 389
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 1 cut(s) 511
HinfI GANTC 4 cut(s) 48, 299, 372, 420
HphI GGTGA 3 cut(s) 60, 308, 451
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 2 cut(s) 163, 322
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 2 cut(s) 143, 280
HpyCH4III ACNGT 3 cut(s) 215, 388, 483
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 1 cut(s) 130
HpyF3I CTNAG 4 cut(s) 276, 282, 426, 537
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 1 cut(s) 511
Kzo9I GATC 2 cut(s) 169, 235
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 1 cut(s) 337
MaeI CTAG 3 cut(s) 41, 515, 544
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 3 cut(s) 242, 421, 457
MalI GATC 2 cut(s) 171, 237
MboI GATC 2 cut(s) 169, 235
MboII GAAGA 9 cut(s) 19, 47, 88, 93, 98, 101, 158, 364, 506
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 1 cut(s) 412
MluCI AATT 2 cut(s) 360, 498
MluNI TGGCCA 1 cut(s) 412
MlyI GAGTC 2 cut(s) 42, 429
MnlI CCTC 5 cut(s) 76, 348, 421, 460, 532
Mox20I TGGCCA 1 cut(s) 412
MscI TGGCCA 1 cut(s) 412
MseI TTAA 1 cut(s) 359
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 412
MspR9I CCNGG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 435
NcoI CCATGG 1 cut(s) 507
NdeII GATC 2 cut(s) 169, 235
NlaIII CATG 1 cut(s) 511
NlaIV GGNNCC 2 cut(s) 326, 432
NmuCI GTSAC 2 cut(s) 421, 457
OliI CACNNNNGTG 1 cut(s) 213
PceI AGGCCT 1 cut(s) 542
PfeI GAWTC 2 cut(s) 299, 372
PkrI GCNGC 2 cut(s) 440, 490
PleI GAGTC 2 cut(s) 42, 428
PpsI GAGTC 2 cut(s) 42, 428
PpuMI RGGWCCY 1 cut(s) 431
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 433
PspEI GGTNACC 1 cut(s) 457
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 326, 432
PspPI GGNCC 2 cut(s) 249, 431
PspPPI RGGWCCY 1 cut(s) 431
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 1 cut(s) 359
SatI GCNGC 2 cut(s) 439, 489
Sau3AI GATC 2 cut(s) 169, 235
Sau96I GGNCC 2 cut(s) 249, 431
SchI GAGTC 2 cut(s) 42, 429
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 210
SetI ASST 4 cut(s) 16, 185, 359, 408
SfaNI GCATC 1 cut(s) 337
SinI GGWCC 2 cut(s) 249, 431
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 2 cut(s) 360, 498
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 2 cut(s) 438, 526
SspMI CTAG 3 cut(s) 41, 515, 544
StuI AGGCCT 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 3 cut(s) 215, 388, 483
TaiI ACGT 1 cut(s) 185
TasI AATT 2 cut(s) 360, 498
TauI GCSGC 1 cut(s) 441
TfiI GAWTC 2 cut(s) 299, 372
Tru1I TTAA 1 cut(s) 359
Tru9I TTAA 1 cut(s) 359
TscAI CASTG 5 cut(s) 87, 204, 280, 391, 430
TseFI GTSAC 2 cut(s) 421, 457
TseI GCWGC 1 cut(s) 488
Tsp45I GTSAC 2 cut(s) 421, 457
TspRI CASTG 5 cut(s) 87, 204, 280, 391, 430
VpaK11BI GGWCC 2 cut(s) 249, 431
XapI RAATTY 1 cut(s) 498
XspI CTAG 3 cut(s) 41, 515, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.