Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001585

Physical Location & Seq

Genomic Coordinates Reverse (-)

25504062 .. 25506967

Gene Structure 2906 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1164200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 705 bp

ATGGGTTTTATCAGAAAATCCTTCAGACTCTTCATCTTCTTCTGCCTCCTGGGGATAATGGTAATTCCATCTGCTGCTTCTGAGAAAGTTTCAGTGACTCTCTACTACGAAACTCTGTGTCCGTACTGTGCAGACTTCATTGTCAACCGGCTGGTCAAGATCTTCCAGAACGGTCTCATCTCCGCCGTCAATCTCCGGCTGGTCCCTTGGGGCAACGCCTGGTTCAATCCCGACGGTTCCTTCGCCTGCCAGCATGGATCAGATGAATGTTTGCTCAACACCATTGAGGCCTGCACCATTAGCATCTATCCTGATGTGAATCGGCATTTCACATTTATCCACTGCGTTGAGCGCTTTACATTGGGGGGCAAACACGGTGCTTGGGCCAATTGCTTTGAAATGACAAGATTGGGCACTGAGCCTATCGATTGCTACAACAGTGGAAATGGCTATGTGATTGAAGGAAGCTATGCTAGGGAAACTGCTCAGCTTAAACCTCCACATAGATTTGTGCCATGGGTGATTGTCAATGATAAACCACTTCAAGAGGACTACCAAAACTTTATGGCCTATATTTGTAAGGCTTACAAAGGCACACCTCCACAAGCTTGCGGATCAATTCGTTATAAGACCGAATCAACCGAGCAAGAAAAACCGGTTCCTCAAGTGTGCTTTGCAAACCAAGGAAGGAACTCTTCACATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04612 map04612

Pfam Domains

Protein Families

GILT

Protein Analysis

235

Amino Acids

26.5

Weight (kDa)

7.5

Isoelectric Point (pI)

33.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GILT	PF03227	32 - 134	8.3e-30	Gamma interferon inducible lysosomal thiol reductase (GILT)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001585)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01580
fragaria_vesca	FvH4_6g03531 FvH4_6g07560 FvH4_6g10801
malus_domestica	MD04G1164200.v1.1 MD11G1065000.v1.1
prunus_persica	Prupe.6G283300_v2.0.a1 Prupe.6G283300_v2.0.a1 Prupe.6G283300_v2.0.a1
pyrus_communis	pycom04g14660 pycom11g05510
rosa_chinensis	RchiOBHm_Chr3g0461861 RchiOBHm_Chr3g0461891 RchiOBHm_Chr3g0461941 RchiOBHm_Chr3g0462011 RchiOBHm_Chr5g0071111 RchiOBHm_Chr7g0217861
rosa_laevigata	RLG00000002432
rosa_multiflora	Rmu_sc0000540.1_g000030 Rmu_sc0000791.1_g000011 Rmu_sc0002003.1_g000003 Rmu_sc0003638.1_g000004 Rmu_sc0004530.1_g000001 Rmu_sc0005209.1_g000007 Rmu_sc0034388.1_g000001
rosa_roxburghii	Rroxscaffold_3G00241640 Rroxscaffold_6G00417780
rosa_rugosa	Rorug01G0192300 Rorug07G0171400 Rorug07G0171500 Rorug07G0171600
rosa_samantha	Rh3AG112300 Rh3AG112400 Rh3CG117700 Rh3CG117800 Rh3CG118100 Rh3DG356800 Rh3DG357100 Rh4AG333400 Rh4BG342200 Rh4CG356700 Rh5AG465800 Rh5CG509200 Rh5DG496100 Rh7AG313600 Rh7BG304200 Rh7DG312100
rosa_wichuraiana	Rw0G007880 Rw1G014510 Rw7G026550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	627
AasI	GACNNNNNNGTC	1 cut(s)	140
AciI	CCGC	2 cut(s)	183, 612
AclWI	GGATC	2 cut(s)	265, 622
AcuI	CTGAAG	1 cut(s)	7
AfaI	GTAC	1 cut(s)	125
AfeI	AGCGCT	1 cut(s)	353
AgeI	ACCGGT	1 cut(s)	655
AgsI	TTSAA	4 cut(s)	226, 398, 461, 545
AjnI	CCWGG	2 cut(s)	48, 218
AluBI	AGCT	3 cut(s)	468, 490, 608
AluI	AGCT	3 cut(s)	468, 490, 608
Alw26I	GTCTC	1 cut(s)	179
AlwI	GGATC	2 cut(s)	265, 622
Aor51HI	AGCGCT	1 cut(s)	353
AoxI	GGCC	3 cut(s)	288, 384, 567
ApeKI	GCWGC	1 cut(s)	74
ArsI	GACNNNNNNTTYG	2 cut(s)	224, 256
AsiGI	ACCGGT	1 cut(s)	655
AspLEI	GCGC	1 cut(s)	354
AspS9I	GGNCC	2 cut(s)	202, 384
AsuHPI	GGTGA	1 cut(s)	532
AvaII	GGWCC	1 cut(s)	202
BaeGI	GKGCMC	1 cut(s)	416
BbvI	GCAGC	1 cut(s)	61
BccI	CCATC	1 cut(s)	76
BceAI	ACGGC	1 cut(s)	170
BciT130I	CCWGG	2 cut(s)	50, 220
BcoDI	GTCTC	1 cut(s)	179
BfaI	CTAG	1 cut(s)	474
BfoI	RGCGCY	1 cut(s)	355
BglII	AGATCT	1 cut(s)	159
BisI	GCNGC	1 cut(s)	75
BlpI	GCTNAGC	1 cut(s)	486
BlsI	GCNGC	1 cut(s)	76
Bme1390I	CCNGG	2 cut(s)	50, 220
Bme18I	GGWCC	1 cut(s)	202
BmgT120I	GGNCC	2 cut(s)	202, 384
BmiI	GGNNCC	3 cut(s)	204, 238, 660
BmrFI	CCNGG	2 cut(s)	50, 220
BmsI	GCATC	1 cut(s)	312
Bpu1102I	GCTNAGC	1 cut(s)	486
BpuEI	CTTGAG	1 cut(s)	648
Bsa29I	ATCGAT	1 cut(s)	426
BsaBI	GATNNNNATC	1 cut(s)	318
BsaI	GGTCTC	1 cut(s)	179
BsaJI	CCNNGG	4 cut(s)	49, 206, 515, 682
BsaWI	WCCGGW	1 cut(s)	655
Bse118I	RCCGGY	2 cut(s)	147, 655
Bse8I	GATNNNNATC	1 cut(s)	318
BseBI	CCWGG	2 cut(s)	50, 220
BseCI	ATCGAT	1 cut(s)	426
BseDI	CCNNGG	4 cut(s)	49, 206, 515, 682
BseJI	GATNNNNATC	1 cut(s)	318
BseMII	CTCAG	3 cut(s)	72, 408, 500
BseSI	GKGCMC	1 cut(s)	416
BseXI	GCAGC	1 cut(s)	61
BsgI	GTGCAG	2 cut(s)	150, 277
BshFI	GGCC	3 cut(s)	290, 386, 569
BshTI	ACCGGT	1 cut(s)	655
BshVI	ATCGAT	1 cut(s)	426
BsiSI	CCGG	3 cut(s)	148, 196, 656
BslFI	GGGAC	1 cut(s)	188
BsmAI	GTCTC	1 cut(s)	179
BsmFI	GGGAC	1 cut(s)	188
BsnI	GGCC	3 cut(s)	290, 386, 569
Bso31I	GGTCTC	1 cut(s)	179
Bsp1286I	GDGCHC	1 cut(s)	416
Bsp143I	GATC	3 cut(s)	159, 257, 614
Bsp1720I	GCTNAGC	1 cut(s)	486
Bsp19I	CCATGG	1 cut(s)	515
BspACI	CCGC	2 cut(s)	183, 612
BspANI	GGCC	3 cut(s)	290, 386, 569
BspCNI	CTCAG	3 cut(s)	73, 409, 499
BspDI	ATCGAT	1 cut(s)	426
BspLI	GGNNCC	3 cut(s)	204, 238, 660
BspPI	GGATC	2 cut(s)	265, 622
BspTNI	GGTCTC	1 cut(s)	179
BsrFI	RCCGGY	2 cut(s)	147, 655
BssAI	RCCGGY	2 cut(s)	147, 655
BssECI	CCNNGG	4 cut(s)	49, 206, 515, 682
BssMI	GATC	3 cut(s)	159, 257, 614
BssT1I	CCWWGG	3 cut(s)	206, 515, 682
Bst2UI	CCWGG	2 cut(s)	50, 220
Bst4CI	ACNGT	5 cut(s)	128, 173, 236, 377, 440
Bst6I	CTCTTC	2 cut(s)	35, 700
BstC8I	GCNNGC	4 cut(s)	247, 251, 292, 610
BstDEI	CTNAG	3 cut(s)	81, 417, 486
BstDSI	CCRYGG	1 cut(s)	515
BstH2I	RGCGCY	1 cut(s)	355
BstHHI	GCGC	1 cut(s)	354
BstKTI	GATC	3 cut(s)	162, 260, 617
BstMAI	GTCTC	1 cut(s)	179
BstMBI	GATC	3 cut(s)	159, 257, 614
BstMWI	GCNNNNNNNGC	2 cut(s)	300, 351
BstNI	CCWGG	2 cut(s)	50, 220
BstSCI	CCNGG	2 cut(s)	48, 218
BstSLI	GKGCMC	1 cut(s)	416
BstV1I	GCAGC	1 cut(s)	61
BstX2I	RGATCY	1 cut(s)	159
BstYI	RGATCY	1 cut(s)	159
Bsu15I	ATCGAT	1 cut(s)	426
BsuRI	GGCC	3 cut(s)	290, 386, 569
BsuTUI	ATCGAT	1 cut(s)	426
BtgI	CCRYGG	1 cut(s)	515
BtsI	GCAGTG	1 cut(s)	340
BtsIMutI	CAGTG	4 cut(s)	99, 340, 414, 445
Cac8I	GCNNGC	4 cut(s)	247, 251, 292, 610
CfoI	GCGC	1 cut(s)	354
Cfr10I	RCCGGY	2 cut(s)	147, 655
Cfr13I	GGNCC	2 cut(s)	202, 384
ClaI	ATCGAT	1 cut(s)	426
Csp6I	GTAC	1 cut(s)	124
CspAI	ACCGGT	1 cut(s)	655
CviAII	CATG	2 cut(s)	254, 516
CviQI	GTAC	1 cut(s)	124
DdeI	CTNAG	3 cut(s)	81, 417, 486
DpnI	GATC	3 cut(s)	161, 259, 616
DpnII	GATC	3 cut(s)	159, 257, 614
DrdI	GACNNNNNNGTC	1 cut(s)	140
DseDI	GACNNNNNNGTC	1 cut(s)	140
Eam1104I	CTCTTC	2 cut(s)	35, 700
EarI	CTCTTC	2 cut(s)	35, 700
EciI	GGCGGA	1 cut(s)	172
Eco130I	CCWWGG	3 cut(s)	206, 515, 682
Eco147I	AGGCCT	1 cut(s)	290
Eco31I	GGTCTC	1 cut(s)	179
Eco47I	GGWCC	1 cut(s)	202
Eco47III	AGCGCT	1 cut(s)	353
Eco57I	CTGAAG	1 cut(s)	7
EcoRII	CCWGG	2 cut(s)	48, 218
EcoT14I	CCWWGG	3 cut(s)	206, 515, 682
ErhI	CCWWGG	3 cut(s)	206, 515, 682
FaeI	CATG	2 cut(s)	257, 519
FaiI	YATR	8 cut(s)	255, 453, 471, 504, 517, 566, 573, 627
FalI	AAGNNNNNCTT	1 cut(s)	679
FaqI	GGGAC	1 cut(s)	188
FatI	CATG	2 cut(s)	253, 515
Fnu4HI	GCNGC	1 cut(s)	75
Fsp4HI	GCNGC	1 cut(s)	75
FspBI	CTAG	1 cut(s)	474
GlaI	GCGC	1 cut(s)	353
GluI	GCNGC	1 cut(s)	75
HaeII	RGCGCY	1 cut(s)	355
HaeIII	GGCC	3 cut(s)	290, 386, 569
HapII	CCGG	3 cut(s)	148, 196, 656
HhaI	GCGC	1 cut(s)	354
Hin1II	CATG	2 cut(s)	257, 519
Hin6I	GCGC	1 cut(s)	352
HinP1I	GCGC	1 cut(s)	352
HincII	GTYRAC	1 cut(s)	145
HindII	GTYRAC	1 cut(s)	145
HindIII	AAGCTT	1 cut(s)	606
HinfI	GANTC	4 cut(s)	27, 97, 319, 635
HpaII	CCGG	3 cut(s)	148, 196, 656
HphI	GGTGA	1 cut(s)	532
Hpy166II	GTNNAC	1 cut(s)	145
Hpy188I	TCNGA	4 cut(s)	14, 26, 82, 262
Hpy188III	TCNNGA	5 cut(s)	157, 166, 230, 311, 545
Hpy8I	GTNNAC	1 cut(s)	145
Hpy99I	CGWCG	1 cut(s)	236
HpyAV	CCTTC	4 cut(s)	31, 250, 455, 681
HpyCH4III	ACNGT	5 cut(s)	128, 173, 236, 377, 440
HpyCH4V	TGCA	3 cut(s)	131, 294, 677
HpyF10VI	GCNNNNNNNGC	2 cut(s)	300, 351
HpyF3I	CTNAG	3 cut(s)	81, 417, 486
Hsp92II	CATG	2 cut(s)	257, 519
HspAI	GCGC	1 cut(s)	352
Kzo9I	GATC	3 cut(s)	159, 257, 614
Lsp1109I	GCAGC	1 cut(s)	61
LweI	GCATC	1 cut(s)	312
MaeI	CTAG	1 cut(s)	474
MaeIII	GTNAC	1 cut(s)	94
MalI	GATC	3 cut(s)	161, 259, 616
MboI	GATC	3 cut(s)	159, 257, 614
MboII	GAAGA	5 cut(s)	22, 28, 31, 154, 687
MfeI	CAATTG	1 cut(s)	388
MflI	RGATCY	1 cut(s)	159
MhlI	GDGCHC	1 cut(s)	416
MluCI	AATT	3 cut(s)	63, 388, 618
MlyI	GAGTC	2 cut(s)	21, 91
MnlI	CCTC	6 cut(s)	56, 280, 507, 541, 609, 672
MseI	TTAA	2 cut(s)	492, 703
MspI	CCGG	3 cut(s)	148, 196, 656
MspR9I	CCNGG	2 cut(s)	50, 220
MunI	CAATTG	1 cut(s)	388
MvaI	CCWGG	2 cut(s)	50, 220
MwoI	GCNNNNNNNGC	2 cut(s)	300, 351
NcoI	CCATGG	1 cut(s)	515
NdeII	GATC	3 cut(s)	159, 257, 614
NlaIII	CATG	2 cut(s)	257, 519
NlaIV	GGNNCC	3 cut(s)	204, 238, 660
NmuCI	GTSAC	1 cut(s)	94
PceI	AGGCCT	1 cut(s)	290
PfeI	GAWTC	2 cut(s)	319, 635
PinAI	ACCGGT	1 cut(s)	655
PkrI	GCNGC	1 cut(s)	76
PleI	GAGTC	2 cut(s)	21, 91
PpsI	GAGTC	2 cut(s)	21, 91
PsiI	TTATAA	1 cut(s)	627
Psp6I	CCWGG	2 cut(s)	48, 218
PspGI	CCWGG	2 cut(s)	48, 218
PspN4I	GGNNCC	3 cut(s)	204, 238, 660
PspPI	GGNCC	2 cut(s)	202, 384
PsuI	RGATCY	1 cut(s)	159
RsaI	GTAC	1 cut(s)	125
RsaNI	GTAC	1 cut(s)	124
SaqAI	TTAA	2 cut(s)	492, 703
SatI	GCNGC	1 cut(s)	75
Sau3AI	GATC	3 cut(s)	159, 257, 614
Sau96I	GGNCC	2 cut(s)	202, 384
SchI	GAGTC	2 cut(s)	21, 91
ScrFI	CCNGG	2 cut(s)	50, 220
SduI	GDGCHC	1 cut(s)	416
SetI	ASST	5 cut(s)	470, 492, 499, 601, 610
SfaNI	GCATC	1 cut(s)	312
SinI	GGWCC	1 cut(s)	202
SmlI	CTYRAG	1 cut(s)	663
SmoI	CTYRAG	1 cut(s)	663
Sse9I	AATT	3 cut(s)	63, 388, 618
SseBI	AGGCCT	1 cut(s)	290
SsiI	CCGC	2 cut(s)	183, 612
SspMI	CTAG	1 cut(s)	474
StuI	AGGCCT	1 cut(s)	290
StyD4I	CCNGG	2 cut(s)	48, 218
StyI	CCWWGG	3 cut(s)	206, 515, 682
TaaI	ACNGT	5 cut(s)	128, 173, 236, 377, 440
TaqI	TCGA	1 cut(s)	426
TaqII	GACCGA	1 cut(s)	647
TasI	AATT	3 cut(s)	63, 388, 618
TfiI	GAWTC	2 cut(s)	319, 635
Tru1I	TTAA	2 cut(s)	492, 703
Tru9I	TTAA	2 cut(s)	492, 703
TscAI	CASTG	4 cut(s)	99, 347, 421, 445
TseFI	GTSAC	1 cut(s)	94
TseI	GCWGC	1 cut(s)	74
Tsp45I	GTSAC	1 cut(s)	94
TspDTI	ATGAA	3 cut(s)	22, 127, 279
TspGWI	ACGGA	1 cut(s)	111
TspRI	CASTG	4 cut(s)	99, 347, 421, 445
VpaK11BI	GGWCC	1 cut(s)	202
XspI	CTAG	1 cut(s)	474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.