MD11G1065000.v1.1
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001585
Physical Location & Seq
Forward (+)
5583477 .. 5585122
1646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1065000.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGCCTCCTCATTTACTCCTTCTTTCCTCCACCTCCACCTCCTCTTCCTCATCTGCTCCGTTTCGCTCTCTCCAACCTCCGCCGCTTATTCCGAGAGCAGAAGCAGTAGCAACAAAGTCAGAGTGGAGCTCTACTATGAAACGCTTTGTCCCGACAGTTATCAATTCATTGTCAATGATCTGATCAAGCTCTTCGAAACCGGAATCATCTCCGTCGCCGATCTGAAGCTCTATCCCTACGGCAACGCCAGGGTTGGATCCAACAGCACCATCACTTGCCAGGATTCACAAATGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

100

Amino Acids

10.95

Weight (kDa)

5.47

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 42 - 96 2.3e-12 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 81, 84
AclWI GGATC 2 cut(s) 252, 265
AcuI CTGAAG 1 cut(s) 245
AjnI CCWGG 2 cut(s) 248, 279
AluBI AGCT 3 cut(s) 130, 190, 229
AluI AGCT 3 cut(s) 130, 190, 229
Alw21I GWGCWC 1 cut(s) 132
AlwI GGATC 2 cut(s) 252, 265
AoxI GGCC 1 cut(s) 3
AsuII TTCGAA 1 cut(s) 195
BamHI GGATCC 1 cut(s) 257
BanII GRGCYC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 132
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 2 cut(s) 250, 281
BclI TGATCA 1 cut(s) 183
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 250, 281
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 2 cut(s) 250, 281
BplI GAGNNNNNCTC 2 cut(s) 114, 146
Bpu14I TTCGAA 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 200
BseBI CCWGG 2 cut(s) 250, 281
BseDI CCNNGG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 32
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 135
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 4 cut(s) 178, 183, 220, 257
BspACI CCGC 2 cut(s) 81, 84
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 2 cut(s) 252, 265
BspQI GCTCTTC 1 cut(s) 197
BspT104I TTCGAA 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 4 cut(s) 178, 183, 220, 257
Bst2UI CCWGG 2 cut(s) 250, 281
Bst4CI ACNGT 1 cut(s) 158
Bst6I CTCTTC 2 cut(s) 50, 197
BstBI TTCGAA 1 cut(s) 195
BstKTI GATC 4 cut(s) 181, 186, 223, 260
BstMBI GATC 4 cut(s) 178, 183, 220, 257
BstNI CCWGG 2 cut(s) 250, 281
BstSCI CCNGG 2 cut(s) 248, 279
BstX2I RGATCY 1 cut(s) 257
BstYI RGATCY 1 cut(s) 257
BsuRI GGCC 1 cut(s) 5
CviJI RGCY 4 cut(s) 5, 130, 190, 229
CviKI_1 RGCY 4 cut(s) 5, 130, 190, 229
DpnI GATC 4 cut(s) 180, 185, 222, 259
DpnII GATC 4 cut(s) 178, 183, 220, 257
Eam1104I CTCTTC 2 cut(s) 50, 197
EarI CTCTTC 2 cut(s) 50, 197
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 248, 279
EcoT38I GRGCYC 1 cut(s) 132
FaiI YATR 1 cut(s) 138
FaqI GGGAC 1 cut(s) 135
FbaI TGATCA 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 84
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 84
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 201
HinfI GANTC 2 cut(s) 204, 284
HpaII CCGG 1 cut(s) 201
Hpy188I TCNGA 4 cut(s) 94, 122, 183, 225
Hpy188III TCNNGA 1 cut(s) 152
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 183
Kzo9I GATC 4 cut(s) 178, 183, 220, 257
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 2 cut(s) 62, 127
LpnPI CCDG 5 cut(s) 214, 235, 262, 266, 293
MalI GATC 4 cut(s) 180, 185, 222, 259
MboI GATC 4 cut(s) 178, 183, 220, 257
MboII GAAGA 2 cut(s) 37, 184
MflI RGATCY 1 cut(s) 257
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 1 cut(s) 164
MmeI TCCRAC 3 cut(s) 98, 235, 285
MnlI CCTC 8 cut(s) 16, 19, 38, 44, 50, 53, 59, 88
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 2 cut(s) 250, 281
MvaI CCWGG 2 cut(s) 250, 281
NdeII GATC 4 cut(s) 178, 183, 220, 257
NlaIV GGNNCC 1 cut(s) 259
NspV TTCGAA 1 cut(s) 195
PciSI GCTCTTC 1 cut(s) 197
PfeI GAWTC 2 cut(s) 204, 284
PkrI GCNGC 1 cut(s) 85
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 2 cut(s) 248, 279
PspGI CCWGG 2 cut(s) 248, 279
PspN4I GGNNCC 1 cut(s) 259
PsuI RGATCY 1 cut(s) 257
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 197
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 4 cut(s) 178, 183, 220, 257
ScrFI CCNGG 2 cut(s) 250, 281
SduI GDGCHC 1 cut(s) 132
SetI ASST 6 cut(s) 36, 42, 80, 132, 192, 231
SfuI TTCGAA 1 cut(s) 195
SgeI CNNG 9 cut(s) 106, 164, 199, 213, 261, 262, 288, 292, 293
Sse9I AATT 1 cut(s) 164
SsiI CCGC 2 cut(s) 81, 84
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 2 cut(s) 248, 279
TaaI ACNGT 1 cut(s) 158
TaqI TCGA 1 cut(s) 195
TasI AATT 1 cut(s) 164
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 2 cut(s) 204, 284
TspDTI ATGAA 2 cut(s) 153, 157
TspGWI ACGGA 2 cut(s) 49, 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.