MD06G1137300.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000824
Physical Location & Seq
Forward (+)
28237372 .. 28237890
519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1137300.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGATATCATAAAGAGAAAGTGGAAGAAGAATATGATCGCCAACGCATGGAAGAGATGTAGTTTACAAAGAGAGAGCAGCAGCAAAGTACTCAACTCATTGACTAAGAGCAAGTCATGCCACTGCAGTTCCACTACTAGGAAGAGCAAAGGCCAGGTAGCTCCGGACGGGTGTTTTTCAGTCTACGTTGGACCACAAAGACAACGGTTTGTAGTGAAGACGGAGTTTGCAAATCATCCGTTGTTTAAGATGCTGCTGGATGATGCCGAAATGGAATACGGTTACAACTGCGACGGTCCGATTTTGCTTCCTTGTGATGTGGATTTGTTTTATAATGTGTTGGCTGAGATGGAGAGCAGTGAGGAAACTAGTACTCCTAATTGTGGGTTTGTTAAGGGTTATGGTTCGTTGATGCTTTGCAGTCCTTCTCGTCGTCTAAATTCAAGCATCAACGACGGTTATGGTGGTGCTTATAGGCTGCTTAGCCCGTTGAAAATGCTTAAGATAAACCAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

173

Amino Acids

19.39

Weight (kDa)

9.11

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 16 - 115 4.9e-24 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AccB7I CCANNNNNTGG 1 cut(s) 48
AccI GTMKAC 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 163
AcsI RAATTY 1 cut(s) 439
AfaI GTAC 2 cut(s) 90, 373
AfiI CCNNNNNNNGG 3 cut(s) 48, 138, 383
AflII CTTAAG 1 cut(s) 500
AgsI TTSAA 2 cut(s) 444, 493
AhlI ACTAGT 1 cut(s) 368
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 1 cut(s) 161
AluI AGCT 1 cut(s) 161
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 4 cut(s) 78, 81, 253, 478
ApoI RAATTY 1 cut(s) 439
AspS9I GGNCC 2 cut(s) 191, 296
AvaII GGWCC 2 cut(s) 191, 296
BbsI GAAGAC 1 cut(s) 224
BbvI GCAGC 4 cut(s) 90, 93, 240, 465
BccI CCATC 1 cut(s) 343
BciT130I CCWGG 1 cut(s) 155
BcuI ACTAGT 1 cut(s) 368
BfaI CTAG 2 cut(s) 138, 369
BfmI CTRYAG 1 cut(s) 124
BfrI CTTAAG 1 cut(s) 500
BisI GCNGC 4 cut(s) 79, 82, 254, 479
BlpI GCTNAGC 1 cut(s) 482
BlsI GCNGC 4 cut(s) 80, 83, 255, 480
BmcAI AGTACT 2 cut(s) 90, 373
Bme1390I CCNGG 1 cut(s) 155
Bme18I GGWCC 2 cut(s) 191, 296
BmgT120I GGNCC 2 cut(s) 191, 296
BmrFI CCNGG 1 cut(s) 155
BmsI GCATC 4 cut(s) 240, 253, 402, 456
BpiI GAAGAC 1 cut(s) 224
Bpu1102I GCTNAGC 1 cut(s) 482
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 138, 383
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 1 cut(s) 155
BseGI GGATG 2 cut(s) 235, 265
BseLI CCNNNNNNNGG 3 cut(s) 48, 138, 383
BseMII CTCAG 1 cut(s) 336
BseXI GCAGC 4 cut(s) 90, 93, 240, 465
BshFI GGCC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 164
BslI CCNNNNNNNGG 3 cut(s) 48, 138, 383
BsnI GGCC 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 1 cut(s) 36
Bsp1720I GCTNAGC 1 cut(s) 482
BspANI GGCC 1 cut(s) 153
BspCNI CTCAG 1 cut(s) 337
BspEI TCCGGA 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 128
BspQI GCTCTTC 1 cut(s) 137
BspTI CTTAAG 1 cut(s) 500
BssMI GATC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 4 cut(s) 207, 281, 296, 458
Bst6I CTCTTC 2 cut(s) 47, 137
BstAFI CTTAAG 1 cut(s) 500
BstAPI GCANNNNNTGC 1 cut(s) 117
BstDEI CTNAG 3 cut(s) 105, 345, 482
BstF5I GGATG 2 cut(s) 235, 265
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 153
BstSFI CTRYAG 1 cut(s) 124
BstV1I GCAGC 4 cut(s) 90, 93, 240, 465
BstV2I GAAGAC 1 cut(s) 224
BsuRI GGCC 1 cut(s) 153
BtsCI GGATG 2 cut(s) 235, 265
BtsI GCAGTG 2 cut(s) 121, 364
BtsIMutI CAGTG 2 cut(s) 121, 364
Cfr13I GGNCC 2 cut(s) 191, 296
CpoI CGGWCCG 1 cut(s) 296
Csp6I GTAC 2 cut(s) 89, 372
CspI CGGWCCG 1 cut(s) 296
CviAII CATG 2 cut(s) 48, 117
CviJI RGCY 5 cut(s) 153, 161, 344, 478, 486
CviKI_1 RGCY 5 cut(s) 153, 161, 344, 478, 486
CviQI GTAC 2 cut(s) 89, 372
DdeI CTNAG 3 cut(s) 105, 345, 482
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
Eam1104I CTCTTC 2 cut(s) 47, 137
EarI CTCTTC 2 cut(s) 47, 137
Eco32I GATATC 1 cut(s) 7
Eco47I GGWCC 2 cut(s) 191, 296
EcoRII CCWGG 1 cut(s) 153
EcoRV GATATC 1 cut(s) 7
FaeI CATG 2 cut(s) 51, 120
FaiI YATR 8 cut(s) 11, 35, 49, 118, 333, 402, 462, 474
FatI CATG 2 cut(s) 47, 116
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 79, 82, 254, 479
FokI GGATG 2 cut(s) 222, 272
Fsp4HI GCNGC 4 cut(s) 79, 82, 254, 479
FspBI CTAG 2 cut(s) 138, 369
GluI GCNGC 4 cut(s) 79, 82, 254, 479
HaeIII GGCC 1 cut(s) 153
HapII CCGG 1 cut(s) 164
Hin1II CATG 2 cut(s) 51, 120
HpaII CCGG 1 cut(s) 164
Hpy166II GTNNAC 2 cut(s) 65, 184
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 2 cut(s) 65, 184
Hpy99I CGWCG 3 cut(s) 296, 435, 458
HpyAV CCTTC 1 cut(s) 435
HpyCH4III ACNGT 4 cut(s) 207, 281, 296, 458
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 3 cut(s) 126, 230, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 3 cut(s) 105, 345, 482
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 2 cut(s) 51, 120
Kpn2I TCCGGA 1 cut(s) 163
Kzo9I GATC 1 cut(s) 36
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 4 cut(s) 140, 167, 177, 242
Lsp1109I GCAGC 4 cut(s) 90, 93, 240, 465
LweI GCATC 4 cut(s) 240, 253, 402, 456
MaeI CTAG 2 cut(s) 138, 369
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 281
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 5 cut(s) 37, 40, 64, 154, 229
MluCI AATT 3 cut(s) 379, 439, 512
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 1 cut(s) 355
MroI TCCGGA 1 cut(s) 163
MseI TTAA 4 cut(s) 246, 393, 501, 517
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 1 cut(s) 36
NlaIII CATG 2 cut(s) 51, 120
PciSI GCTCTTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 4 cut(s) 80, 83, 255, 480
PsiI TTATAA 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 153
PspGI CCWGG 1 cut(s) 153
PspPI GGNCC 2 cut(s) 191, 296
PstI CTGCAG 1 cut(s) 128
RsaI GTAC 2 cut(s) 90, 373
RsaNI GTAC 2 cut(s) 89, 372
Rsr2I CGGWCCG 1 cut(s) 296
RsrII CGGWCCG 1 cut(s) 296
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 4 cut(s) 246, 393, 501, 517
SatI GCNGC 4 cut(s) 79, 82, 254, 479
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 2 cut(s) 191, 296
ScaI AGTACT 2 cut(s) 90, 373
ScrFI CCNGG 1 cut(s) 155
SetI ASST 3 cut(s) 159, 163, 189
SfaNI GCATC 4 cut(s) 240, 253, 402, 456
SfcI CTRYAG 1 cut(s) 124
SinI GGWCC 2 cut(s) 191, 296
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SpeI ACTAGT 1 cut(s) 368
Sse9I AATT 3 cut(s) 379, 439, 512
SspMI CTAG 2 cut(s) 138, 369
StyD4I CCNGG 1 cut(s) 153
TaaI ACNGT 4 cut(s) 207, 281, 296, 458
TaiI ACGT 1 cut(s) 189
TasI AATT 3 cut(s) 379, 439, 512
TatI WGTACW 2 cut(s) 88, 371
Tru1I TTAA 4 cut(s) 246, 393, 501, 517
Tru9I TTAA 4 cut(s) 246, 393, 501, 517
TscAI CASTG 2 cut(s) 128, 364
TseI GCWGC 4 cut(s) 78, 81, 253, 478
TspGWI ACGGA 2 cut(s) 228, 236
TspRI CASTG 2 cut(s) 128, 364
Van91I CCANNNNNTGG 1 cut(s) 48
Vha464I CTTAAG 1 cut(s) 500
VpaK11BI GGWCC 2 cut(s) 191, 296
XapI RAATTY 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 2 cut(s) 138, 369
ZrmI AGTACT 2 cut(s) 90, 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.