MD14G1152100.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000824
Physical Location & Seq
Forward (+)
24578067 .. 24578540
474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1152100.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGATATCATAAAGGGAAAGTGGAAGAAGAATCTGATCGCCAACGCATGGAAACGATGGAGTTTACAAAGAAAGAGCAGCAGCAAAGTACACAACTCATTGACAAAGAGCAAGTCACGGCACTGCAGCTCCAATACTAGGAAGAGCAAAGGCCAGGTAGCTCCGGACGGGTGTTTTTCGGTCTATGTTGGACCGCAAAGACAACGGTTTGCAGTGAAGACGGAGTTTGCTAATCATCCCTTGTTTAAGATGCTGCTGGATGATGCCGAAATGGAATACGGTTACAATTGCGAAGGTCCGATTTTGCTTCCTTGTGATGTGGATTTGTTTTATAAAGTGTTGGCTGAGATGGAGAGCAGTGAGGAAGTTAGTACTCCTAATTGTGGGTTTGTTAAGGGTTATGGTTCGTTGATGCTTTGCAGCCCGGCACGTCGTCTAAATTCTTGCATCCATGACGCTTGGCGGTGCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

158

Amino Acids

17.84

Weight (kDa)

9.24

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 21 - 115 1.7e-23 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 465
AccB7I CCANNNNNTGG 1 cut(s) 48
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 2 cut(s) 194, 463
AcsI RAATTY 1 cut(s) 439
AfaI GTAC 2 cut(s) 90, 373
AfiI CCNNNNNNNGG 3 cut(s) 48, 138, 383
AjiI CACGTC 1 cut(s) 431
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 2 cut(s) 129, 161
AluI AGCT 2 cut(s) 129, 161
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 5 cut(s) 78, 81, 126, 253, 420
ApoI RAATTY 1 cut(s) 439
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AspS9I GGNCC 2 cut(s) 191, 296
AsuC2I CCSGG 1 cut(s) 425
AvaII GGWCC 2 cut(s) 191, 296
BanI GGYRCC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 224
BbvI GCAGC 5 cut(s) 90, 93, 138, 240, 432
BccI CCATC 2 cut(s) 51, 343
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 1 cut(s) 155
BcnI CCSGG 1 cut(s) 425
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 2 cut(s) 124, 470
BisI GCNGC 5 cut(s) 79, 82, 127, 254, 421
BlsI GCNGC 5 cut(s) 80, 83, 128, 255, 422
BmcAI AGTACT 1 cut(s) 373
Bme1390I CCNGG 2 cut(s) 155, 425
Bme18I GGWCC 2 cut(s) 191, 296
BmgBI CACGTC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 191, 296
BmiI GGNNCC 1 cut(s) 467
BmrFI CCNGG 2 cut(s) 155, 425
BmsI GCATC 4 cut(s) 240, 253, 402, 456
BpiI GAAGAC 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 425
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 138, 383
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 1 cut(s) 155
BseGI GGATG 3 cut(s) 235, 265, 447
BseLI CCNNNNNNNGG 3 cut(s) 48, 138, 383
BseMII CTCAG 1 cut(s) 336
BseXI GCAGC 5 cut(s) 90, 93, 138, 240, 432
BshFI GGCC 1 cut(s) 153
BshNI GGYRCC 1 cut(s) 465
BsiSI CCGG 2 cut(s) 164, 425
BslI CCNNNNNNNGG 3 cut(s) 48, 138, 383
BsnI GGCC 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 2 cut(s) 194, 463
BspANI GGCC 1 cut(s) 153
BspCNI CTCAG 1 cut(s) 337
BspEI TCCGGA 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 467
BspMAI CTGCAG 1 cut(s) 128
BspQI GCTCTTC 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 465
BssMI GATC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 2 cut(s) 207, 281
Bst6I CTCTTC 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 345
BstF5I GGATG 3 cut(s) 235, 265, 447
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 153, 423
BstSFI CTRYAG 2 cut(s) 124, 470
BstV1I GCAGC 5 cut(s) 90, 93, 138, 240, 432
BstV2I GAAGAC 1 cut(s) 224
BsuRI GGCC 1 cut(s) 153
BtrI CACGTC 1 cut(s) 431
BtsCI GGATG 3 cut(s) 235, 265, 447
BtsI GCAGTG 3 cut(s) 121, 219, 364
BtsIMutI CAGTG 3 cut(s) 121, 219, 364
Cfr13I GGNCC 2 cut(s) 191, 296
CseI GACGC 1 cut(s) 464
Csp6I GTAC 2 cut(s) 89, 372
CviAII CATG 2 cut(s) 48, 452
CviJI RGCY 5 cut(s) 129, 153, 161, 344, 423
CviKI_1 RGCY 5 cut(s) 129, 153, 161, 344, 423
CviQI GTAC 2 cut(s) 89, 372
DdeI CTNAG 1 cut(s) 345
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Eco32I GATATC 1 cut(s) 7
Eco47I GGWCC 2 cut(s) 191, 296
EcoRII CCWGG 1 cut(s) 153
EcoRV GATATC 1 cut(s) 7
FaeI CATG 2 cut(s) 51, 455
FaiI YATR 7 cut(s) 11, 49, 186, 333, 402, 453, 472
FatI CATG 2 cut(s) 47, 451
Fnu4HI GCNGC 5 cut(s) 79, 82, 127, 254, 421
FokI GGATG 3 cut(s) 222, 272, 434
Fsp4HI GCNGC 5 cut(s) 79, 82, 127, 254, 421
FspBI CTAG 1 cut(s) 138
GluI GCNGC 5 cut(s) 79, 82, 127, 254, 421
HaeIII GGCC 1 cut(s) 153
HapII CCGG 2 cut(s) 164, 425
HgaI GACGC 1 cut(s) 464
Hin1II CATG 2 cut(s) 51, 455
HinfI GANTC 1 cut(s) 31
HpaII CCGG 2 cut(s) 164, 425
Hpy166II GTNNAC 2 cut(s) 65, 91
Hpy188I TCNGA 2 cut(s) 36, 300
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 2 cut(s) 65, 91
Hpy99I CGWCG 1 cut(s) 435
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 2 cut(s) 207, 281
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 4 cut(s) 126, 212, 420, 447
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 1 cut(s) 430
Hsp92II CATG 2 cut(s) 51, 455
Kpn2I TCCGGA 1 cut(s) 163
Kzo9I GATC 1 cut(s) 36
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 2 cut(s) 134, 166
LpnPI CCDG 5 cut(s) 140, 167, 177, 242, 438
Lsp1109I GCAGC 5 cut(s) 90, 93, 138, 240, 432
LweI GCATC 4 cut(s) 240, 253, 402, 456
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 430
MaeIII GTNAC 2 cut(s) 114, 281
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 4 cut(s) 37, 40, 154, 229
MfeI CAATTG 1 cut(s) 286
MluCI AATT 3 cut(s) 286, 379, 439
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 1 cut(s) 355
MroI TCCGGA 1 cut(s) 163
MseI TTAA 2 cut(s) 246, 393
MspI CCGG 2 cut(s) 164, 425
MspR9I CCNGG 2 cut(s) 155, 425
MunI CAATTG 1 cut(s) 286
MvaI CCWGG 1 cut(s) 155
NciI CCSGG 1 cut(s) 425
NdeII GATC 1 cut(s) 36
NlaIII CATG 2 cut(s) 51, 455
NlaIV GGNNCC 1 cut(s) 467
NmuCI GTSAC 1 cut(s) 114
PciSI GCTCTTC 1 cut(s) 137
PfeI GAWTC 1 cut(s) 31
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 5 cut(s) 80, 83, 128, 255, 422
PsiI TTATAA 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 153
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 467
PspPI GGNCC 2 cut(s) 191, 296
PstI CTGCAG 1 cut(s) 128
RsaI GTAC 2 cut(s) 90, 373
RsaNI GTAC 2 cut(s) 89, 372
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 2 cut(s) 246, 393
SatI GCNGC 5 cut(s) 79, 82, 127, 254, 421
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 2 cut(s) 191, 296
ScaI AGTACT 1 cut(s) 373
ScrFI CCNGG 2 cut(s) 155, 425
SetI ASST 5 cut(s) 131, 159, 163, 298, 433
SfaNI GCATC 4 cut(s) 240, 253, 402, 456
SfcI CTRYAG 2 cut(s) 124, 470
SinI GGWCC 2 cut(s) 191, 296
Sse9I AATT 3 cut(s) 286, 379, 439
SsiI CCGC 2 cut(s) 194, 463
SspMI CTAG 1 cut(s) 138
StyD4I CCNGG 2 cut(s) 153, 423
TaaI ACNGT 2 cut(s) 207, 281
TaiI ACGT 1 cut(s) 433
TaqII GACCGA 1 cut(s) 169
TasI AATT 3 cut(s) 286, 379, 439
TatI WGTACW 2 cut(s) 88, 371
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 2 cut(s) 246, 393
Tru9I TTAA 2 cut(s) 246, 393
TscAI CASTG 3 cut(s) 128, 219, 364
TseFI GTSAC 1 cut(s) 114
TseI GCWGC 5 cut(s) 78, 81, 126, 253, 420
Tsp45I GTSAC 1 cut(s) 114
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 3 cut(s) 128, 219, 364
Van91I CCANNNNNTGG 1 cut(s) 48
VpaK11BI GGWCC 2 cut(s) 191, 296
XapI RAATTY 1 cut(s) 439
XspI CTAG 1 cut(s) 138
ZrmI AGTACT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.