Rh7DG105200

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000824
Physical Location & Seq
Forward (+)
8990272 .. 8990926
655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG105200.1

Sequence Viewer

Length: 489 bp
ATGGAAATGGTGAAGGCAGAGTCGACAAAGAATCTGATTATCAAGGCGTGGAGGCTATGCACTACTTTACATAAACAAACCAGCAGCAAAGGGTGCACAGCACCCAGTTCAAGGAGTTGCAGTACTAGCAATGGCAAGCGGAAGAAGAACAAGGCCGACGAAGTAACTCCTGTCGGGTGTTTCTCAGTCTACGTCGGACCCGAAAAACAACGGTTTGTGGTGAAGGTGGAGTTTGCTAATCATCCGTTGTTCAAGGTGCTGTTAGAGGATGCAGCATTGGAGTATGGGTACAAAAATGATGGTCCACTGTTGCTTCCTTGTGATGTGGATTTGTTCTACAATGTTTTGGCAGAGATGGAGAGCATGGATAACATCAATGAGGAAATCATAAGTTCCAACCGGTCTTTGATACAAATTTTCTTCAGTCCAAATAGCAGTCATGCATCATTCAAATTCAAGAATGGACAAGGGTTTTGGTGGTGGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

162

Amino Acids

18.35

Weight (kDa)

8.57

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 36 - 117 2.8e-22 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 23, 189
AciI CCGC 1 cut(s) 139
AcsI RAATTY 2 cut(s) 414, 452
AcuI CTGAAG 1 cut(s) 406
AfaI GTAC 2 cut(s) 124, 290
AfiI CCNNNNNNNGG 1 cut(s) 111
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 4 cut(s) 111, 253, 451, 457
Alw21I GWGCWC 1 cut(s) 98
Alw44I GTGCAC 1 cut(s) 94
AoxI GGCC 1 cut(s) 153
ApaLI GTGCAC 1 cut(s) 94
ApeKI GCWGC 2 cut(s) 84, 272
ApoI RAATTY 2 cut(s) 414, 452
ArsI GACNNNNNNTTYG 2 cut(s) 456, 488
AsiGI ACCGGT 1 cut(s) 399
AspS9I GGNCC 2 cut(s) 197, 302
AsuHPI GGTGA 2 cut(s) 22, 232
AvaII GGWCC 2 cut(s) 197, 302
BaeGI GKGCMC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 2 cut(s) 96, 284
BccI CCATC 2 cut(s) 293, 349
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 1 cut(s) 485
BisI GCNGC 2 cut(s) 85, 273
BlsI GCNGC 2 cut(s) 86, 274
BmcAI AGTACT 1 cut(s) 124
Bme18I GGWCC 2 cut(s) 197, 302
BmgT120I GGNCC 2 cut(s) 197, 302
BmiI GGNNCC 1 cut(s) 199
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 2 cut(s) 259, 452
BmuI ACTGGG 1 cut(s) 99
BsaWI WCCGGW 1 cut(s) 399
BsaXI ACNNNNNCTCC 4 cut(s) 106, 136, 272, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse118I RCCGGY 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 136
BseGI GGATG 2 cut(s) 241, 274
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 105
BseSI GKGCMC 1 cut(s) 98
BseXI GCAGC 2 cut(s) 96, 284
BshFI GGCC 1 cut(s) 155
BshTI ACCGGT 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 1 cut(s) 400
BslI CCNNNNNNNGG 1 cut(s) 111
BsnI GGCC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 98
BspACI CCGC 1 cut(s) 139
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 399
BsrI ACTGG 1 cut(s) 105
BssAI RCCGGY 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 213, 309
BstAPI GCANNNNNTGC 1 cut(s) 93
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 241, 274
BstMWI GCNNNNNNNGC 2 cut(s) 93, 126
BstSFI CTRYAG 1 cut(s) 485
BstSLI GKGCMC 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 96, 284
BsuRI GGCC 1 cut(s) 155
BtsCI GGATG 2 cut(s) 241, 274
BtsIMutI CAGTG 1 cut(s) 305
Cac8I GCNNGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 197, 302
Csp6I GTAC 2 cut(s) 123, 289
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 2 cut(s) 364, 440
CviJI RGCY 3 cut(s) 55, 155, 484
CviKI_1 RGCY 3 cut(s) 55, 155, 484
CviQI GTAC 2 cut(s) 123, 289
DdeI CTNAG 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 197, 302
Eco57I CTGAAG 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 445
FaeI CATG 2 cut(s) 367, 443
FaiI YATR 7 cut(s) 58, 72, 285, 365, 389, 441, 487
FatI CATG 2 cut(s) 363, 439
FblI GTMKAC 2 cut(s) 23, 189
Fnu4HI GCNGC 2 cut(s) 85, 273
FokI GGATG 2 cut(s) 228, 281
Fsp4HI GCNGC 2 cut(s) 85, 273
FspBI CTAG 1 cut(s) 126
GluI GCNGC 2 cut(s) 85, 273
HaeIII GGCC 1 cut(s) 155
HapII CCGG 1 cut(s) 400
Hin1II CATG 2 cut(s) 367, 443
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 2 cut(s) 20, 31
HpaII CCGG 1 cut(s) 400
HphI GGTGA 2 cut(s) 22, 232
Hpy166II GTNNAC 4 cut(s) 24, 96, 190, 305
Hpy188I TCNGA 2 cut(s) 36, 197
Hpy188III TCNNGA 1 cut(s) 457
Hpy8I GTNNAC 4 cut(s) 24, 96, 190, 305
Hpy99I CGWCG 2 cut(s) 161, 197
HpyAV CCTTC 2 cut(s) 7, 217
HpyCH4III ACNGT 2 cut(s) 213, 309
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 5 cut(s) 60, 96, 120, 272, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 126
HpyF3I CTNAG 1 cut(s) 184
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 2 cut(s) 367, 443
LpnPI CCDG 4 cut(s) 94, 118, 183, 413
Lsp1109I GCAGC 2 cut(s) 96, 284
LweI GCATC 2 cut(s) 259, 452
MaeI CTAG 1 cut(s) 126
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 163
MboII GAAGA 3 cut(s) 154, 157, 412
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 2 cut(s) 414, 452
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 2 cut(s) 175, 420
MnlI CCTC 3 cut(s) 45, 259, 373
Mph1103I ATGCAT 1 cut(s) 445
MspI CCGG 1 cut(s) 400
MwoI GCNNNNNNNGC 2 cut(s) 93, 126
NlaIII CATG 2 cut(s) 367, 443
NlaIV GGNNCC 1 cut(s) 199
NsiI ATGCAT 1 cut(s) 445
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 1 cut(s) 31
PinAI ACCGGT 1 cut(s) 399
PkrI GCNGC 2 cut(s) 86, 274
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 199
PspPI GGNCC 2 cut(s) 197, 302
RsaI GTAC 2 cut(s) 124, 290
RsaNI GTAC 2 cut(s) 123, 289
SalI GTCGAC 1 cut(s) 22
SatI GCNGC 2 cut(s) 85, 273
Sau96I GGNCC 2 cut(s) 197, 302
ScaI AGTACT 1 cut(s) 124
SchI GAGTC 1 cut(s) 29
SduI GDGCHC 1 cut(s) 98
SetI ASST 3 cut(s) 195, 228, 258
SfaNI GCATC 2 cut(s) 259, 452
SfcI CTRYAG 1 cut(s) 485
SinI GGWCC 2 cut(s) 197, 302
Sse9I AATT 2 cut(s) 414, 452
SsiI CCGC 1 cut(s) 139
SspMI CTAG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 213, 309
TaiI ACGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 23
TasI AATT 2 cut(s) 414, 452
TatI WGTACW 1 cut(s) 122
TfiI GAWTC 1 cut(s) 31
TscAI CASTG 1 cut(s) 312
TseI GCWGC 2 cut(s) 84, 272
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 312
VneI GTGCAC 1 cut(s) 94
VpaK11BI GGWCC 2 cut(s) 197, 302
XapI RAATTY 2 cut(s) 414, 452
XmiI GTMKAC 2 cut(s) 23, 189
XspI CTAG 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 124
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.